Acinetobacter phage vB_AbaM_IME284

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Obolenskvirus

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 84 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3BXS3|A0A3G3BXS3_9CAUD Uncharacterized protein OS=Acinetobacter phage vB_AbaM_IME284 OX=2419625 PE=4 SV=1
MM1 pKa = 7.25AQYY4 pKa = 9.85DD5 pKa = 3.73IPLAQVPNQFFTTSLNGVTWSITLEE30 pKa = 3.98TRR32 pKa = 11.84LNNLYY37 pKa = 10.27ISLSNNNDD45 pKa = 2.72GDD47 pKa = 3.93VLLNRR52 pKa = 11.84ICLNRR57 pKa = 11.84TYY59 pKa = 10.65LGYY62 pKa = 11.24GFIFVDD68 pKa = 3.2IDD70 pKa = 3.72GNNDD74 pKa = 3.33PEE76 pKa = 4.4YY77 pKa = 9.83TGLGTRR83 pKa = 11.84YY84 pKa = 10.14LLIWTDD90 pKa = 3.71EE91 pKa = 4.13VV92 pKa = 3.51

Molecular weight: 10.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3BXS6|A0A3G3BXS6_9CAUD Uncharacterized protein OS=Acinetobacter phage vB_AbaM_IME284 OX=2419625 PE=4 SV=1
MM1 pKa = 7.27NPIAMILLSFALSFVFVVVLYY22 pKa = 10.63LYY24 pKa = 9.64EE25 pKa = 4.25AYY27 pKa = 9.53KK28 pKa = 10.26RR29 pKa = 11.84KK30 pKa = 10.12RR31 pKa = 11.84YY32 pKa = 7.46VQRR35 pKa = 11.84KK36 pKa = 8.76QDD38 pKa = 3.67DD39 pKa = 3.52QRR41 pKa = 11.84KK42 pKa = 8.64FNPIRR47 pKa = 11.84EE48 pKa = 4.3KK49 pKa = 10.66QWWKK53 pKa = 10.98

Molecular weight: 6.66 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

84

0

84

13134

37

682

156.4

17.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.02 ± 0.418

1.203 ± 0.133

6.061 ± 0.274

6.693 ± 0.392

4.584 ± 0.259

7.035 ± 0.305

1.705 ± 0.152

6.868 ± 0.273

7.637 ± 0.403

8.17 ± 0.29

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.81 ± 0.162

5.68 ± 0.28

3.19 ± 0.176

3.99 ± 0.234

3.571 ± 0.241

6.51 ± 0.213

5.497 ± 0.319

6.495 ± 0.281

1.515 ± 0.122

3.769 ± 0.247
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski