Streptococcus phage Javan562

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6BFI0|A0A4D6BFI0_9CAUD Minor tail protein OS=Streptococcus phage Javan562 OX=2548258 GN=Javan562_0046 PE=4 SV=1
MM1 pKa = 7.73TEE3 pKa = 4.06TTNNTLLDD11 pKa = 4.22LSTITEE17 pKa = 4.24PFDD20 pKa = 3.42LATALVYY27 pKa = 10.15MKK29 pKa = 10.63EE30 pKa = 3.65NGEE33 pKa = 4.45FIRR36 pKa = 11.84CKK38 pKa = 10.46SATQDD43 pKa = 2.84FYY45 pKa = 11.15MYY47 pKa = 10.54RR48 pKa = 11.84DD49 pKa = 3.67VQKK52 pKa = 10.76RR53 pKa = 11.84PAVVDD58 pKa = 3.4GRR60 pKa = 11.84RR61 pKa = 11.84QYY63 pKa = 11.8VDD65 pKa = 3.67VEE67 pKa = 4.74SVWAYY72 pKa = 6.06TQWGGISTSINVADD86 pKa = 4.35LFKK89 pKa = 11.03ADD91 pKa = 4.18FYY93 pKa = 11.3IMKK96 pKa = 9.78FDD98 pKa = 4.43EE99 pKa = 4.87NGNPDD104 pKa = 3.05WSDD107 pKa = 3.32PTVEE111 pKa = 3.9AA112 pKa = 4.97

Molecular weight: 12.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6BEJ0|A0A4D6BEJ0_9CAUD Uncharacterized protein OS=Streptococcus phage Javan562 OX=2548258 GN=Javan562_0015 PE=4 SV=1
MM1 pKa = 7.54RR2 pKa = 11.84PKK4 pKa = 10.2RR5 pKa = 11.84YY6 pKa = 9.0PYY8 pKa = 9.64SGQMKK13 pKa = 9.46KK14 pKa = 9.94QPHH17 pKa = 6.49DD18 pKa = 4.43EE19 pKa = 4.06IAKK22 pKa = 9.54LRR24 pKa = 11.84CEE26 pKa = 4.18VAVNTSEE33 pKa = 4.51LSNIKK38 pKa = 10.26RR39 pKa = 11.84KK40 pKa = 10.05LSSNN44 pKa = 3.49

Molecular weight: 5.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

10381

44

1399

195.9

22.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.388 ± 0.472

0.578 ± 0.081

6.396 ± 0.315

7.205 ± 0.43

3.766 ± 0.256

6.473 ± 0.438

1.474 ± 0.147

6.56 ± 0.27

8.188 ± 0.437

8.275 ± 0.288

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.514 ± 0.278

5.587 ± 0.245

2.928 ± 0.228

3.988 ± 0.227

4.479 ± 0.236

6.348 ± 0.377

6.127 ± 0.444

7.071 ± 0.317

1.204 ± 0.12

3.449 ± 0.246
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski