Lactobacillus prophage Lj928

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 50 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q9AZA2|Q9AZA2_9CAUD Orf309 OS=Lactobacillus prophage Lj928 OX=139872 GN=orf309 PE=4 SV=1
MM1 pKa = 7.53SFNNHH6 pKa = 4.07VFEE9 pKa = 5.09YY10 pKa = 10.61KK11 pKa = 10.8CFDD14 pKa = 3.38NKK16 pKa = 10.21IEE18 pKa = 3.99YY19 pKa = 9.16LACKK23 pKa = 10.08DD24 pKa = 5.08PEE26 pKa = 4.4YY27 pKa = 10.6EE28 pKa = 3.73WKK30 pKa = 10.75NRR32 pKa = 11.84INYY35 pKa = 9.48LFDD38 pKa = 4.52RR39 pKa = 11.84SSSTLSISGDD49 pKa = 3.04FGFAVFCWYY58 pKa = 10.57SSQNTLRR65 pKa = 11.84DD66 pKa = 3.14IAEE69 pKa = 3.98YY70 pKa = 10.88SKK72 pKa = 11.34DD73 pKa = 2.95LGYY76 pKa = 10.46FVSKK80 pKa = 10.54VKK82 pKa = 10.41AAEE85 pKa = 4.11EE86 pKa = 4.0LWSYY90 pKa = 11.37DD91 pKa = 3.27YY92 pKa = 11.8SLLDD96 pKa = 3.6EE97 pKa = 4.43QLNDD101 pKa = 3.58YY102 pKa = 11.03LLLSDD107 pKa = 5.51EE108 pKa = 5.41NDD110 pKa = 3.25DD111 pKa = 4.5CYY113 pKa = 11.25LSKK116 pKa = 10.78SEE118 pKa = 3.97RR119 pKa = 11.84QEE121 pKa = 3.87LKK123 pKa = 11.08NSILEE128 pKa = 4.17SWDD131 pKa = 3.35DD132 pKa = 3.27RR133 pKa = 11.84TGYY136 pKa = 10.77HH137 pKa = 5.93MSSDD141 pKa = 4.49LEE143 pKa = 4.1TKK145 pKa = 10.19IAVEE149 pKa = 4.14FDD151 pKa = 3.15PDD153 pKa = 3.31YY154 pKa = 11.16WEE156 pKa = 4.64NLPDD160 pKa = 3.91GKK162 pKa = 11.05VISDD166 pKa = 4.52WIRR169 pKa = 11.84DD170 pKa = 3.85YY171 pKa = 11.88ASGLQKK177 pKa = 10.22WLEE180 pKa = 3.81QDD182 pKa = 3.73TII184 pKa = 3.82

Molecular weight: 21.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6SEG0|Q6SEG0_9CAUD Uncharacterized protein OS=Lactobacillus prophage Lj928 OX=139872 GN=Ljo_1461 PE=4 SV=1
MM1 pKa = 7.81RR2 pKa = 11.84SFIRR6 pKa = 11.84KK7 pKa = 8.69PSLKK11 pKa = 10.08KK12 pKa = 10.67SFSARR17 pKa = 11.84TKK19 pKa = 10.3GRR21 pKa = 11.84ATRR24 pKa = 11.84SMKK27 pKa = 10.64RR28 pKa = 11.84MINPTYY34 pKa = 9.87GKK36 pKa = 9.93KK37 pKa = 8.82GTGMLTNPKK46 pKa = 10.01KK47 pKa = 10.74SMYY50 pKa = 10.01NKK52 pKa = 9.65VYY54 pKa = 10.3SHH56 pKa = 6.44TSISAMPKK64 pKa = 9.18GSKK67 pKa = 10.07KK68 pKa = 10.39KK69 pKa = 10.09ISRR72 pKa = 11.84SHH74 pKa = 7.71SDD76 pKa = 3.42DD77 pKa = 3.86GCWGCGCLIIFMIIVFIVCLLVV99 pKa = 3.31

Molecular weight: 11.13 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

50

0

50

11552

48

1544

231.0

26.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.15 ± 0.72

0.545 ± 0.133

6.83 ± 0.415

5.809 ± 0.301

3.636 ± 0.263

5.878 ± 0.437

1.679 ± 0.218

6.795 ± 0.335

9.401 ± 0.472

7.973 ± 0.3

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.45 ± 0.163

6.44 ± 0.235

2.961 ± 0.268

4.597 ± 0.237

3.618 ± 0.259

7.306 ± 0.615

6.224 ± 0.282

5.549 ± 0.233

1.229 ± 0.165

3.93 ± 0.379
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski