Pseudorhodobacter sp. PARRP1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Pseudorhodobacter; unclassified Pseudorhodobacter

Average proteome isoelectric point is 6.48

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4111 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A257GIE0|A0A257GIE0_9RHOB ABC transporter permease OS=Pseudorhodobacter sp. PARRP1 OX=2015560 GN=CFE33_16535 PE=3 SV=1
MM1 pKa = 7.51PVISSSVSVSAINAHH16 pKa = 6.6ALTIAVAGNYY26 pKa = 9.97LITGPTYY33 pKa = 10.8LEE35 pKa = 4.55SEE37 pKa = 4.01SSYY40 pKa = 11.58AVGVNAGIGPVTINLAGSIVASLSYY65 pKa = 10.72YY66 pKa = 10.74GIYY69 pKa = 9.96QPGATDD75 pKa = 3.91PLTVNILSSGSIFGGAVVFATSQLALTNDD104 pKa = 3.71GYY106 pKa = 11.65LYY108 pKa = 10.88GQDD111 pKa = 3.61SGIASAPTATTNDD124 pKa = 3.32SVINRR129 pKa = 11.84GEE131 pKa = 3.92IFTTAGLAIDD141 pKa = 4.7LGGGADD147 pKa = 3.36VLVNSGHH154 pKa = 5.83ITGDD158 pKa = 3.32VNLGVGDD165 pKa = 3.67DD166 pKa = 3.48RR167 pKa = 11.84FYY169 pKa = 11.63GGGGSVLGTLDD180 pKa = 4.36LSTGNDD186 pKa = 3.89LIDD189 pKa = 4.38LRR191 pKa = 11.84GAQIDD196 pKa = 3.97GSVYY200 pKa = 10.64GGDD203 pKa = 3.7GNDD206 pKa = 3.21VFIVDD211 pKa = 4.53DD212 pKa = 4.45ASVDD216 pKa = 4.36LIEE219 pKa = 5.0YY220 pKa = 9.18SAQGTDD226 pKa = 3.45LVKK229 pKa = 10.4STVSYY234 pKa = 9.58EE235 pKa = 3.72LRR237 pKa = 11.84VNFEE241 pKa = 3.99NLTLLGAGDD250 pKa = 3.76INGTGNAAANTLTGNAGDD268 pKa = 4.2NRR270 pKa = 11.84LHH272 pKa = 7.32GYY274 pKa = 7.71TGNDD278 pKa = 3.06KK279 pKa = 11.02AYY281 pKa = 9.8GGSGDD286 pKa = 3.75DD287 pKa = 5.02VIYY290 pKa = 10.7GDD292 pKa = 3.56QGNDD296 pKa = 3.0VLYY299 pKa = 11.05GGIGVDD305 pKa = 4.1LLYY308 pKa = 11.16GGAGNDD314 pKa = 3.15ILRR317 pKa = 11.84GEE319 pKa = 4.27TGEE322 pKa = 4.28DD323 pKa = 3.31RR324 pKa = 11.84LIGGAGADD332 pKa = 3.83TLTGDD337 pKa = 3.12VGTAGGYY344 pKa = 10.69DD345 pKa = 3.47DD346 pKa = 5.57VFVFQRR352 pKa = 11.84VADD355 pKa = 3.97MPNAGSLDD363 pKa = 4.9LITDD367 pKa = 3.64FHH369 pKa = 8.26IGEE372 pKa = 4.47DD373 pKa = 4.18KK374 pKa = 10.77IDD376 pKa = 4.02LSAIDD381 pKa = 4.39AKK383 pKa = 11.11AGTLANDD390 pKa = 3.58AFSFVASFTSVAGQLIKK407 pKa = 10.44QASGLDD413 pKa = 3.55TLILGDD419 pKa = 3.68INGDD423 pKa = 3.26GVADD427 pKa = 4.37FRR429 pKa = 11.84ILLAGNVAVTAADD442 pKa = 4.89FILL445 pKa = 4.91

Molecular weight: 44.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A257GLE2|A0A257GLE2_9RHOB Flagellar hook-basal body protein FliE OS=Pseudorhodobacter sp. PARRP1 OX=2015560 GN=CFE33_13180 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84TRR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.63GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.34GGRR28 pKa = 11.84LVLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 8.83KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4111

0

4111

1288030

28

2742

313.3

33.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.526 ± 0.06

0.871 ± 0.011

5.705 ± 0.028

5.035 ± 0.033

3.648 ± 0.023

8.804 ± 0.048

2.062 ± 0.023

5.124 ± 0.027

3.279 ± 0.033

10.284 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.783 ± 0.019

2.62 ± 0.022

5.14 ± 0.027

3.392 ± 0.021

6.33 ± 0.038

5.022 ± 0.029

5.476 ± 0.032

7.276 ± 0.034

1.421 ± 0.018

2.201 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski