Neisseria subflava

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales;

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1949 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D7WJF4|A0A4D7WJF4_NEISU Extensin OS=Neisseria subflava OX=28449 GN=FAH66_09380 PE=4 SV=1
MM1 pKa = 7.95RR2 pKa = 11.84GTDD5 pKa = 3.63AKK7 pKa = 10.51DD8 pKa = 3.47TIYY11 pKa = 9.58GTAGEE16 pKa = 4.42DD17 pKa = 3.25IIYY20 pKa = 10.02GGNGADD26 pKa = 3.88VIYY29 pKa = 10.75AGAGNDD35 pKa = 4.11TIYY38 pKa = 11.41GDD40 pKa = 3.98ADD42 pKa = 3.53GDD44 pKa = 4.03SLYY47 pKa = 11.32GEE49 pKa = 4.37EE50 pKa = 5.01GKK52 pKa = 10.39DD53 pKa = 3.51YY54 pKa = 11.25LQGGDD59 pKa = 3.68GNDD62 pKa = 3.64YY63 pKa = 11.39LNGGSGADD71 pKa = 3.23IMRR74 pKa = 11.84GGDD77 pKa = 3.48GNDD80 pKa = 2.92VYY82 pKa = 11.05FVDD85 pKa = 4.17NADD88 pKa = 3.89DD89 pKa = 3.82QVIEE93 pKa = 4.02YY94 pKa = 10.71GNLNGGIDD102 pKa = 3.74TVRR105 pKa = 11.84TVIDD109 pKa = 3.42YY110 pKa = 8.21TLTDD114 pKa = 3.31NVEE117 pKa = 3.96NLFLQGMQNLNGTGNSLNNNIEE139 pKa = 4.15GNGGNNHH146 pKa = 7.22LYY148 pKa = 10.76GLAGDD153 pKa = 4.33DD154 pKa = 4.14CLVGKK159 pKa = 10.11DD160 pKa = 3.69GNDD163 pKa = 3.49YY164 pKa = 11.3LDD166 pKa = 3.98GGVGNDD172 pKa = 3.34ILIGGTGNDD181 pKa = 3.15TYY183 pKa = 11.21FFDD186 pKa = 4.76KK187 pKa = 11.04GYY189 pKa = 10.19GHH191 pKa = 6.69DD192 pKa = 4.01TIRR195 pKa = 11.84EE196 pKa = 3.93EE197 pKa = 4.61SGNDD201 pKa = 3.02TLLFGKK207 pKa = 10.37GIAASDD213 pKa = 3.69VLLSKK218 pKa = 10.79SGANLTVSVGADD230 pKa = 3.82RR231 pKa = 11.84ITIDD235 pKa = 4.98DD236 pKa = 3.91WFTGNNHH243 pKa = 5.67KK244 pKa = 10.75VEE246 pKa = 4.14NFKK249 pKa = 10.87FADD252 pKa = 3.81GSTYY256 pKa = 10.61QVTGHH261 pKa = 6.57GDD263 pKa = 3.79YY264 pKa = 11.28YY265 pKa = 11.3SLSAVNHH272 pKa = 5.8IQEE275 pKa = 4.25QTQVSGWW282 pKa = 3.33

Molecular weight: 29.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D7WFU3|A0A4D7WFU3_NEISU Uroporphyrinogen-III synthase OS=Neisseria subflava OX=28449 GN=FAH66_03085 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.14QPSVTKK11 pKa = 10.56RR12 pKa = 11.84KK13 pKa = 7.91RR14 pKa = 11.84THH16 pKa = 5.89GFLVRR21 pKa = 11.84SKK23 pKa = 9.38TRR25 pKa = 11.84GGRR28 pKa = 11.84AVLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.87GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1949

0

1949

600927

30

1578

308.3

34.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.984 ± 0.077

0.984 ± 0.017

5.294 ± 0.047

6.248 ± 0.06

4.061 ± 0.04

7.313 ± 0.065

2.265 ± 0.029

5.91 ± 0.044

5.634 ± 0.053

10.08 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.523 ± 0.03

4.192 ± 0.047

4.164 ± 0.036

4.255 ± 0.049

5.051 ± 0.052

5.607 ± 0.045

5.314 ± 0.043

6.826 ± 0.051

1.269 ± 0.025

3.028 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski