Amino acid dipepetide frequency for Synechococcus phage S-B05

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.188AlaAla: 4.188 ± 0.362
0.452AlaCys: 0.452 ± 0.09
3.737AlaAsp: 3.737 ± 0.256
3.052AlaGlu: 3.052 ± 0.281
2.273AlaPhe: 2.273 ± 0.186
5.589AlaGly: 5.589 ± 0.443
0.934AlaHis: 0.934 ± 0.131
3.192AlaIle: 3.192 ± 0.186
3.379AlaLys: 3.379 ± 0.356
4.266AlaLeu: 4.266 ± 0.304
1.074AlaMet: 1.074 ± 0.165
3.924AlaAsn: 3.924 ± 0.279
2.678AlaPro: 2.678 ± 0.172
2.538AlaGln: 2.538 ± 0.188
2.351AlaArg: 2.351 ± 0.221
4.593AlaSer: 4.593 ± 0.275
4.951AlaThr: 4.951 ± 0.346
3.441AlaVal: 3.441 ± 0.267
0.498AlaTrp: 0.498 ± 0.091
2.476AlaTyr: 2.476 ± 0.186
0.0AlaXaa: 0.0 ± 0.0
Cys
0.607CysAla: 0.607 ± 0.125
0.109CysCys: 0.109 ± 0.045
0.592CysAsp: 0.592 ± 0.106
0.732CysGlu: 0.732 ± 0.127
0.389CysPhe: 0.389 ± 0.072
0.669CysGly: 0.669 ± 0.124
0.187CysHis: 0.187 ± 0.055
0.576CysIle: 0.576 ± 0.094
0.576CysLys: 0.576 ± 0.135
0.623CysLeu: 0.623 ± 0.111
0.28CysMet: 0.28 ± 0.076
0.389CysAsn: 0.389 ± 0.086
0.327CysPro: 0.327 ± 0.087
0.311CysGln: 0.311 ± 0.078
0.452CysArg: 0.452 ± 0.096
0.576CysSer: 0.576 ± 0.117
0.405CysThr: 0.405 ± 0.073
0.654CysVal: 0.654 ± 0.125
0.125CysTrp: 0.125 ± 0.046
0.374CysTyr: 0.374 ± 0.083
0.0CysXaa: 0.0 ± 0.0
Asp
3.892AspAla: 3.892 ± 0.269
0.638AspCys: 0.638 ± 0.113
4.219AspAsp: 4.219 ± 0.322
3.55AspGlu: 3.55 ± 0.278
3.316AspPhe: 3.316 ± 0.204
5.512AspGly: 5.512 ± 0.265
1.121AspHis: 1.121 ± 0.155
4.313AspIle: 4.313 ± 0.222
3.659AspLys: 3.659 ± 0.28
4.967AspLeu: 4.967 ± 0.304
1.666AspMet: 1.666 ± 0.19
3.69AspAsn: 3.69 ± 0.234
2.444AspPro: 2.444 ± 0.216
1.604AspGln: 1.604 ± 0.148
2.834AspArg: 2.834 ± 0.231
3.939AspSer: 3.939 ± 0.291
3.908AspThr: 3.908 ± 0.291
4.982AspVal: 4.982 ± 0.297
0.825AspTrp: 0.825 ± 0.109
3.285AspTyr: 3.285 ± 0.222
0.0AspXaa: 0.0 ± 0.0
Glu
3.145GluAla: 3.145 ± 0.231
0.561GluCys: 0.561 ± 0.092
4.048GluAsp: 4.048 ± 0.294
5.371GluGlu: 5.371 ± 0.528
2.834GluPhe: 2.834 ± 0.226
3.908GluGly: 3.908 ± 0.26
0.856GluHis: 0.856 ± 0.145
4.033GluIle: 4.033 ± 0.27
4.001GluLys: 4.001 ± 0.377
4.936GluLeu: 4.936 ± 0.298
1.619GluMet: 1.619 ± 0.186
3.55GluAsn: 3.55 ± 0.249
1.744GluPro: 1.744 ± 0.147
2.476GluGln: 2.476 ± 0.302
2.834GluArg: 2.834 ± 0.249
3.643GluSer: 3.643 ± 0.25
3.643GluThr: 3.643 ± 0.253
4.749GluVal: 4.749 ± 0.296
0.732GluTrp: 0.732 ± 0.12
2.6GluTyr: 2.6 ± 0.2
0.0GluXaa: 0.0 ± 0.0
Phe
2.226PheAla: 2.226 ± 0.187
0.545PheCys: 0.545 ± 0.106
3.519PheAsp: 3.519 ± 0.249
2.787PheGlu: 2.787 ± 0.23
1.464PhePhe: 1.464 ± 0.154
3.098PheGly: 3.098 ± 0.222
0.685PheHis: 0.685 ± 0.11
2.507PheIle: 2.507 ± 0.19
2.912PheLys: 2.912 ± 0.228
3.02PheLeu: 3.02 ± 0.214
1.09PheMet: 1.09 ± 0.137
2.771PheAsn: 2.771 ± 0.246
1.355PhePro: 1.355 ± 0.152
1.666PheGln: 1.666 ± 0.148
1.899PheArg: 1.899 ± 0.152
3.41PheSer: 3.41 ± 0.249
3.456PheThr: 3.456 ± 0.231
3.114PheVal: 3.114 ± 0.271
0.483PheTrp: 0.483 ± 0.082
1.775PheTyr: 1.775 ± 0.165
0.0PheXaa: 0.0 ± 0.0
Gly
5.403GlyAla: 5.403 ± 0.39
0.467GlyCys: 0.467 ± 0.093
4.78GlyAsp: 4.78 ± 0.299
3.861GlyGlu: 3.861 ± 0.213
3.472GlyPhe: 3.472 ± 0.34
7.115GlyGly: 7.115 ± 0.431
1.028GlyHis: 1.028 ± 0.167
6.057GlyIle: 6.057 ± 0.438
3.737GlyLys: 3.737 ± 0.424
5.107GlyLeu: 5.107 ± 0.291
1.759GlyMet: 1.759 ± 0.16
4.811GlyAsn: 4.811 ± 0.3
2.04GlyPro: 2.04 ± 0.179
2.662GlyGln: 2.662 ± 0.24
3.145GlyArg: 3.145 ± 0.205
7.022GlySer: 7.022 ± 0.413
7.302GlyThr: 7.302 ± 0.576
6.088GlyVal: 6.088 ± 0.505
0.996GlyTrp: 0.996 ± 0.12
3.456GlyTyr: 3.456 ± 0.307
0.0GlyXaa: 0.0 ± 0.0
His
0.638HisAla: 0.638 ± 0.106
0.389HisCys: 0.389 ± 0.092
0.701HisAsp: 0.701 ± 0.118
0.81HisGlu: 0.81 ± 0.136
1.012HisPhe: 1.012 ± 0.12
0.996HisGly: 0.996 ± 0.155
0.405HisHis: 0.405 ± 0.091
0.965HisIle: 0.965 ± 0.132
1.09HisLys: 1.09 ± 0.143
1.137HisLeu: 1.137 ± 0.168
0.249HisMet: 0.249 ± 0.073
0.856HisAsn: 0.856 ± 0.123
0.81HisPro: 0.81 ± 0.145
0.592HisGln: 0.592 ± 0.097
0.716HisArg: 0.716 ± 0.129
1.059HisSer: 1.059 ± 0.125
1.121HisThr: 1.121 ± 0.174
0.81HisVal: 0.81 ± 0.119
0.218HisTrp: 0.218 ± 0.072
0.856HisTyr: 0.856 ± 0.13
0.0HisXaa: 0.0 ± 0.0
Ile
3.721IleAla: 3.721 ± 0.261
0.592IleCys: 0.592 ± 0.085
5.013IleAsp: 5.013 ± 0.263
4.609IleGlu: 4.609 ± 0.295
2.398IlePhe: 2.398 ± 0.17
5.278IleGly: 5.278 ± 0.289
0.965IleHis: 0.965 ± 0.131
3.332IleIle: 3.332 ± 0.277
3.986IleLys: 3.986 ± 0.334
4.359IleLeu: 4.359 ± 0.254
1.246IleMet: 1.246 ± 0.155
3.97IleAsn: 3.97 ± 0.233
2.709IlePro: 2.709 ± 0.199
2.149IleGln: 2.149 ± 0.182
2.709IleArg: 2.709 ± 0.217
4.655IleSer: 4.655 ± 0.32
5.231IleThr: 5.231 ± 0.379
3.986IleVal: 3.986 ± 0.373
0.592IleTrp: 0.592 ± 0.098
2.616IleTyr: 2.616 ± 0.191
0.0IleXaa: 0.0 ± 0.0
Lys
3.347LysAla: 3.347 ± 0.44
0.623LysCys: 0.623 ± 0.118
3.908LysAsp: 3.908 ± 0.326
4.11LysGlu: 4.11 ± 0.394
2.787LysPhe: 2.787 ± 0.222
3.472LysGly: 3.472 ± 0.316
0.919LysHis: 0.919 ± 0.14
4.282LysIle: 4.282 ± 0.318
5.294LysLys: 5.294 ± 0.562
4.577LysLeu: 4.577 ± 0.35
1.65LysMet: 1.65 ± 0.262
3.005LysAsn: 3.005 ± 0.228
2.117LysPro: 2.117 ± 0.212
1.962LysGln: 1.962 ± 0.219
2.585LysArg: 2.585 ± 0.261
4.048LysSer: 4.048 ± 0.286
3.752LysThr: 3.752 ± 0.284
3.892LysVal: 3.892 ± 0.283
0.638LysTrp: 0.638 ± 0.123
2.834LysTyr: 2.834 ± 0.253
0.0LysXaa: 0.0 ± 0.0
Leu
4.033LeuAla: 4.033 ± 0.278
0.592LeuCys: 0.592 ± 0.101
5.496LeuAsp: 5.496 ± 0.301
4.718LeuGlu: 4.718 ± 0.361
2.585LeuPhe: 2.585 ± 0.167
5.138LeuGly: 5.138 ± 0.268
1.199LeuHis: 1.199 ± 0.15
3.55LeuIle: 3.55 ± 0.241
4.546LeuLys: 4.546 ± 0.294
4.406LeuLeu: 4.406 ± 0.296
1.573LeuMet: 1.573 ± 0.223
4.624LeuAsn: 4.624 ± 0.265
3.02LeuPro: 3.02 ± 0.189
3.083LeuGln: 3.083 ± 0.171
3.363LeuArg: 3.363 ± 0.222
5.574LeuSer: 5.574 ± 0.311
5.294LeuThr: 5.294 ± 0.4
4.437LeuVal: 4.437 ± 0.237
0.607LeuTrp: 0.607 ± 0.11
2.88LeuTyr: 2.88 ± 0.192
0.0LeuXaa: 0.0 ± 0.0
Met
1.448MetAla: 1.448 ± 0.166
0.265MetCys: 0.265 ± 0.065
1.308MetAsp: 1.308 ± 0.171
1.09MetGlu: 1.09 ± 0.173
0.778MetPhe: 0.778 ± 0.126
1.323MetGly: 1.323 ± 0.199
0.234MetHis: 0.234 ± 0.076
1.573MetIle: 1.573 ± 0.184
1.79MetLys: 1.79 ± 0.27
1.355MetLeu: 1.355 ± 0.156
0.685MetMet: 0.685 ± 0.131
1.137MetAsn: 1.137 ± 0.139
0.95MetPro: 0.95 ± 0.136
0.592MetGln: 0.592 ± 0.086
1.105MetArg: 1.105 ± 0.153
1.588MetSer: 1.588 ± 0.158
1.822MetThr: 1.822 ± 0.188
1.199MetVal: 1.199 ± 0.139
0.28MetTrp: 0.28 ± 0.061
0.841MetTyr: 0.841 ± 0.142
0.0MetXaa: 0.0 ± 0.0
Asn
3.238AsnAla: 3.238 ± 0.21
0.436AsnCys: 0.436 ± 0.089
3.379AsnAsp: 3.379 ± 0.251
3.114AsnGlu: 3.114 ± 0.217
3.238AsnPhe: 3.238 ± 0.282
4.453AsnGly: 4.453 ± 0.357
1.323AsnHis: 1.323 ± 0.167
4.671AsnIle: 4.671 ± 0.372
3.285AsnLys: 3.285 ± 0.271
4.92AsnLeu: 4.92 ± 0.31
0.996AsnMet: 0.996 ± 0.12
4.157AsnAsn: 4.157 ± 0.321
3.114AsnPro: 3.114 ± 0.234
2.398AsnGln: 2.398 ± 0.191
2.024AsnArg: 2.024 ± 0.164
4.282AsnSer: 4.282 ± 0.342
4.033AsnThr: 4.033 ± 0.29
4.624AsnVal: 4.624 ± 0.31
0.701AsnTrp: 0.701 ± 0.111
2.709AsnTyr: 2.709 ± 0.206
0.0AsnXaa: 0.0 ± 0.0
Pro
2.149ProAla: 2.149 ± 0.24
0.296ProCys: 0.296 ± 0.084
2.709ProAsp: 2.709 ± 0.2
2.631ProGlu: 2.631 ± 0.206
1.635ProPhe: 1.635 ± 0.156
3.129ProGly: 3.129 ± 0.239
0.669ProHis: 0.669 ± 0.106
2.086ProIle: 2.086 ± 0.165
2.398ProLys: 2.398 ± 0.222
2.413ProLeu: 2.413 ± 0.198
0.638ProMet: 0.638 ± 0.092
1.962ProAsn: 1.962 ± 0.167
1.355ProPro: 1.355 ± 0.164
1.401ProGln: 1.401 ± 0.177
1.339ProArg: 1.339 ± 0.143
3.129ProSer: 3.129 ± 0.25
2.989ProThr: 2.989 ± 0.175
2.398ProVal: 2.398 ± 0.243
0.483ProTrp: 0.483 ± 0.084
1.79ProTyr: 1.79 ± 0.167
0.0ProXaa: 0.0 ± 0.0
Gln
1.853GlnAla: 1.853 ± 0.215
0.358GlnCys: 0.358 ± 0.091
1.915GlnAsp: 1.915 ± 0.145
2.709GlnGlu: 2.709 ± 0.214
1.713GlnPhe: 1.713 ± 0.175
2.585GlnGly: 2.585 ± 0.213
0.436GlnHis: 0.436 ± 0.085
2.678GlnIle: 2.678 ± 0.192
1.993GlnLys: 1.993 ± 0.181
3.129GlnLeu: 3.129 ± 0.191
0.981GlnMet: 0.981 ± 0.126
2.273GlnAsn: 2.273 ± 0.205
1.51GlnPro: 1.51 ± 0.136
1.51GlnGln: 1.51 ± 0.165
1.728GlnArg: 1.728 ± 0.19
2.569GlnSer: 2.569 ± 0.176
2.195GlnThr: 2.195 ± 0.172
2.46GlnVal: 2.46 ± 0.198
0.343GlnTrp: 0.343 ± 0.072
1.65GlnTyr: 1.65 ± 0.155
0.0GlnXaa: 0.0 ± 0.0
Arg
2.444ArgAla: 2.444 ± 0.223
0.467ArgCys: 0.467 ± 0.104
2.071ArgAsp: 2.071 ± 0.194
2.569ArgGlu: 2.569 ± 0.214
1.868ArgPhe: 1.868 ± 0.146
3.207ArgGly: 3.207 ± 0.239
0.592ArgHis: 0.592 ± 0.103
2.896ArgIle: 2.896 ± 0.202
3.005ArgLys: 3.005 ± 0.341
2.927ArgLeu: 2.927 ± 0.246
1.09ArgMet: 1.09 ± 0.139
2.818ArgAsn: 2.818 ± 0.247
1.214ArgPro: 1.214 ± 0.173
1.619ArgGln: 1.619 ± 0.186
1.79ArgArg: 1.79 ± 0.205
2.211ArgSer: 2.211 ± 0.209
2.008ArgThr: 2.008 ± 0.208
3.41ArgVal: 3.41 ± 0.359
0.436ArgTrp: 0.436 ± 0.081
2.304ArgTyr: 2.304 ± 0.213
0.0ArgXaa: 0.0 ± 0.0
Ser
5.2SerAla: 5.2 ± 0.296
0.483SerCys: 0.483 ± 0.09
4.437SerAsp: 4.437 ± 0.336
3.924SerGlu: 3.924 ± 0.247
3.441SerPhe: 3.441 ± 0.255
7.676SerGly: 7.676 ± 0.504
1.012SerHis: 1.012 ± 0.131
4.78SerIle: 4.78 ± 0.309
3.363SerLys: 3.363 ± 0.247
5.2SerLeu: 5.2 ± 0.314
1.105SerMet: 1.105 ± 0.155
4.749SerAsn: 4.749 ± 0.291
2.32SerPro: 2.32 ± 0.142
2.725SerGln: 2.725 ± 0.231
2.865SerArg: 2.865 ± 0.298
6.181SerSer: 6.181 ± 0.45
6.275SerThr: 6.275 ± 0.492
5.013SerVal: 5.013 ± 0.354
0.701SerTrp: 0.701 ± 0.125
3.067SerTyr: 3.067 ± 0.305
0.0SerXaa: 0.0 ± 0.0
Thr
5.076ThrAla: 5.076 ± 0.394
0.42ThrCys: 0.42 ± 0.08
3.768ThrAsp: 3.768 ± 0.252
3.815ThrGlu: 3.815 ± 0.243
3.768ThrPhe: 3.768 ± 0.285
7.38ThrGly: 7.38 ± 0.567
1.059ThrHis: 1.059 ± 0.116
5.34ThrIle: 5.34 ± 0.392
3.441ThrLys: 3.441 ± 0.247
5.512ThrLeu: 5.512 ± 0.335
1.028ThrMet: 1.028 ± 0.145
4.702ThrAsn: 4.702 ± 0.425
3.285ThrPro: 3.285 ± 0.269
2.662ThrGln: 2.662 ± 0.195
2.367ThrArg: 2.367 ± 0.173
6.461ThrSer: 6.461 ± 0.528
6.819ThrThr: 6.819 ± 0.634
5.356ThrVal: 5.356 ± 0.495
0.872ThrTrp: 0.872 ± 0.097
2.771ThrTyr: 2.771 ± 0.201
0.0ThrXaa: 0.0 ± 0.0
Val
4.141ValAla: 4.141 ± 0.292
0.623ValCys: 0.623 ± 0.116
4.858ValAsp: 4.858 ± 0.283
4.282ValGlu: 4.282 ± 0.255
2.553ValPhe: 2.553 ± 0.232
6.166ValGly: 6.166 ± 0.609
0.887ValHis: 0.887 ± 0.136
3.955ValIle: 3.955 ± 0.261
3.908ValLys: 3.908 ± 0.266
3.768ValLeu: 3.768 ± 0.227
1.355ValMet: 1.355 ± 0.156
3.955ValAsn: 3.955 ± 0.285
2.585ValPro: 2.585 ± 0.183
2.444ValGln: 2.444 ± 0.187
2.943ValArg: 2.943 ± 0.246
5.916ValSer: 5.916 ± 0.467
6.96ValThr: 6.96 ± 0.604
5.06ValVal: 5.06 ± 0.36
0.669ValTrp: 0.669 ± 0.106
2.538ValTyr: 2.538 ± 0.188
0.0ValXaa: 0.0 ± 0.0
Trp
0.623TrpAla: 0.623 ± 0.116
0.14TrpCys: 0.14 ± 0.048
0.747TrpAsp: 0.747 ± 0.126
0.732TrpGlu: 0.732 ± 0.122
0.42TrpPhe: 0.42 ± 0.076
0.747TrpGly: 0.747 ± 0.111
0.156TrpHis: 0.156 ± 0.047
0.81TrpIle: 0.81 ± 0.114
0.794TrpLys: 0.794 ± 0.11
0.763TrpLeu: 0.763 ± 0.125
0.374TrpMet: 0.374 ± 0.074
0.763TrpAsn: 0.763 ± 0.121
0.296TrpPro: 0.296 ± 0.072
0.296TrpGln: 0.296 ± 0.069
0.452TrpArg: 0.452 ± 0.086
0.778TrpSer: 0.778 ± 0.127
0.763TrpThr: 0.763 ± 0.127
0.607TrpVal: 0.607 ± 0.101
0.109TrpTrp: 0.109 ± 0.04
0.436TrpTyr: 0.436 ± 0.087
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.211TyrAla: 2.211 ± 0.2
0.498TyrCys: 0.498 ± 0.122
3.161TyrAsp: 3.161 ± 0.213
2.818TyrGlu: 2.818 ± 0.202
1.837TyrPhe: 1.837 ± 0.224
2.865TyrGly: 2.865 ± 0.251
0.763TyrHis: 0.763 ± 0.1
2.522TyrIle: 2.522 ± 0.186
2.647TyrLys: 2.647 ± 0.207
3.332TyrLeu: 3.332 ± 0.225
0.887TyrMet: 0.887 ± 0.152
2.989TyrAsn: 2.989 ± 0.25
1.728TyrPro: 1.728 ± 0.149
1.915TyrGln: 1.915 ± 0.214
1.448TyrArg: 1.448 ± 0.16
2.88TyrSer: 2.88 ± 0.187
2.974TyrThr: 2.974 ± 0.193
3.285TyrVal: 3.285 ± 0.236
0.498TyrTrp: 0.498 ± 0.114
2.024TyrTyr: 2.024 ± 0.196
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 268 proteins (64229 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski