Auriculariopsis ampla

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes; Agaricomycetidae; Agaricales; Schizophyllaceae; Auriculariopsis

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 15404 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A550CAU4|A0A550CAU4_9AGAR Asparagine synthase (glutamine-hydrolyzing) OS=Auriculariopsis ampla OX=97359 GN=BD626DRAFT_500924 PE=4 SV=1
MM1 pKa = 7.59PSEE4 pKa = 4.24STNLFNLARR13 pKa = 11.84NKK15 pKa = 10.36LHH17 pKa = 6.98LSLGSRR23 pKa = 11.84SDD25 pKa = 3.69CSLHH29 pKa = 6.71RR30 pKa = 11.84WVLLKK35 pKa = 10.97NSIVHH40 pKa = 5.35STPHH44 pKa = 6.4SSSAPDD50 pKa = 3.42VAASDD55 pKa = 3.52AAADD59 pKa = 3.72VRR61 pKa = 11.84NAPDD65 pKa = 3.0TDD67 pKa = 3.81YY68 pKa = 11.1TVEE71 pKa = 4.09EE72 pKa = 4.37EE73 pKa = 4.12VDD75 pKa = 3.59SFMFPDD81 pKa = 4.52ADD83 pKa = 3.9QLIDD87 pKa = 4.01DD88 pKa = 5.13RR89 pKa = 11.84AAEE92 pKa = 4.38GNGSEE97 pKa = 4.29EE98 pKa = 3.86QWLDD102 pKa = 3.44SLLEE106 pKa = 4.2TLSHH110 pKa = 6.57EE111 pKa = 4.4EE112 pKa = 3.84EE113 pKa = 5.21DD114 pKa = 4.48DD115 pKa = 4.0FGMDD119 pKa = 3.54SDD121 pKa = 4.24VHH123 pKa = 8.03SMLAEE128 pKa = 5.02DD129 pKa = 5.84DD130 pKa = 5.35DD131 pKa = 6.2DD132 pKa = 6.74DD133 pKa = 5.66SLLSPSISPMSSSDD147 pKa = 3.59DD148 pKa = 3.46LTRR151 pKa = 11.84PPVYY155 pKa = 9.86YY156 pKa = 9.28PPPIAVPYY164 pKa = 7.79PVPYY168 pKa = 9.89PPYY171 pKa = 9.23QPPPARR177 pKa = 11.84ADD179 pKa = 3.58ALPSPFDD186 pKa = 3.25IHH188 pKa = 7.9RR189 pKa = 11.84MSPYY193 pKa = 10.52LDD195 pKa = 3.85PLPYY199 pKa = 9.94HH200 pKa = 6.88DD201 pKa = 6.14AEE203 pKa = 6.61DD204 pKa = 4.0EE205 pKa = 4.58DD206 pKa = 5.38EE207 pKa = 4.75DD208 pKa = 6.17LSMPDD213 pKa = 4.23AIEE216 pKa = 4.43DD217 pKa = 3.91TSDD220 pKa = 4.21DD221 pKa = 4.09EE222 pKa = 5.09SDD224 pKa = 3.86SPLTPSIGHH233 pKa = 6.17SGSLSQDD240 pKa = 3.48PAQVPLPGEE249 pKa = 3.73RR250 pKa = 11.84SGLRR254 pKa = 11.84EE255 pKa = 3.96HH256 pKa = 6.35VHH258 pKa = 7.2ILVDD262 pKa = 3.45TDD264 pKa = 4.0DD265 pKa = 3.99SYY267 pKa = 11.9FYY269 pKa = 10.58PFAVDD274 pKa = 4.29PLPFPDD280 pKa = 5.36DD281 pKa = 3.63QLQNPYY287 pKa = 8.53NTYY290 pKa = 9.51QEE292 pKa = 4.51CC293 pKa = 3.52

Molecular weight: 32.4 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A550C2I8|A0A550C2I8_9AGAR Protein kinase domain-containing protein OS=Auriculariopsis ampla OX=97359 GN=BD626DRAFT_573039 PE=4 SV=1
MM1 pKa = 7.08SQSTNLTRR9 pKa = 11.84PSSARR14 pKa = 11.84TTHH17 pKa = 6.93TIQLTTAASHH27 pKa = 6.25GAPRR31 pKa = 11.84MTASARR37 pKa = 11.84ALTGKK42 pKa = 10.12SSARR46 pKa = 11.84STPTTTIAASLSKK59 pKa = 10.47RR60 pKa = 11.84RR61 pKa = 11.84NN62 pKa = 3.24

Molecular weight: 6.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

15404

0

15404

6453724

49

6535

419.0

46.16

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.93 ± 0.021

1.42 ± 0.009

5.914 ± 0.016

5.736 ± 0.019

3.384 ± 0.013

6.519 ± 0.022

2.615 ± 0.01

4.203 ± 0.014

3.948 ± 0.018

9.038 ± 0.023

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.256 ± 0.008

2.909 ± 0.009

6.777 ± 0.026

3.578 ± 0.012

7.093 ± 0.02

8.265 ± 0.027

5.944 ± 0.012

6.377 ± 0.015

1.429 ± 0.008

2.663 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski