Amino acid dipepetide frequency for Ralstonia phage Anchaing

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.394AlaAla: 18.394 ± 1.872
1.409AlaCys: 1.409 ± 0.291
6.675AlaAsp: 6.675 ± 0.767
6.823AlaGlu: 6.823 ± 0.929
2.967AlaPhe: 2.967 ± 0.522
7.565AlaGly: 7.565 ± 0.944
2.151AlaHis: 2.151 ± 0.488
5.118AlaIle: 5.118 ± 0.619
5.34AlaLys: 5.34 ± 0.649
11.348AlaLeu: 11.348 ± 1.048
3.931AlaMet: 3.931 ± 0.53
4.895AlaAsn: 4.895 ± 0.611
3.56AlaPro: 3.56 ± 0.628
6.527AlaGln: 6.527 ± 0.852
7.046AlaArg: 7.046 ± 0.803
6.304AlaSer: 6.304 ± 0.507
5.266AlaThr: 5.266 ± 0.918
7.268AlaVal: 7.268 ± 0.666
1.483AlaTrp: 1.483 ± 0.285
3.115AlaTyr: 3.115 ± 0.526
0.0AlaXaa: 0.0 ± 0.0
Cys
0.964CysAla: 0.964 ± 0.298
0.0CysCys: 0.0 ± 0.0
0.593CysAsp: 0.593 ± 0.226
0.297CysGlu: 0.297 ± 0.148
0.148CysPhe: 0.148 ± 0.093
0.593CysGly: 0.593 ± 0.209
0.148CysHis: 0.148 ± 0.113
0.593CysIle: 0.593 ± 0.261
0.371CysLys: 0.371 ± 0.212
0.964CysLeu: 0.964 ± 0.254
0.148CysMet: 0.148 ± 0.139
0.519CysAsn: 0.519 ± 0.204
0.371CysPro: 0.371 ± 0.181
0.297CysGln: 0.297 ± 0.182
0.519CysArg: 0.519 ± 0.208
0.593CysSer: 0.593 ± 0.199
0.223CysThr: 0.223 ± 0.118
1.038CysVal: 1.038 ± 0.299
0.074CysTrp: 0.074 ± 0.072
0.148CysTyr: 0.148 ± 0.107
0.0CysXaa: 0.0 ± 0.0
Asp
6.304AspAla: 6.304 ± 0.742
0.445AspCys: 0.445 ± 0.166
3.857AspAsp: 3.857 ± 0.543
3.486AspGlu: 3.486 ± 0.485
2.818AspPhe: 2.818 ± 0.468
4.302AspGly: 4.302 ± 0.572
0.742AspHis: 0.742 ± 0.264
3.189AspIle: 3.189 ± 0.425
1.928AspLys: 1.928 ± 0.435
4.079AspLeu: 4.079 ± 0.726
2.003AspMet: 2.003 ± 0.384
2.151AspAsn: 2.151 ± 0.408
4.45AspPro: 4.45 ± 0.458
1.706AspGln: 1.706 ± 0.301
3.783AspArg: 3.783 ± 0.458
2.077AspSer: 2.077 ± 0.433
3.412AspThr: 3.412 ± 0.429
3.634AspVal: 3.634 ± 0.487
1.261AspTrp: 1.261 ± 0.252
2.077AspTyr: 2.077 ± 0.32
0.0AspXaa: 0.0 ± 0.0
Glu
7.194GluAla: 7.194 ± 0.821
0.445GluCys: 0.445 ± 0.176
2.893GluAsp: 2.893 ± 0.429
3.041GluGlu: 3.041 ± 0.586
1.706GluPhe: 1.706 ± 0.315
2.967GluGly: 2.967 ± 0.487
1.261GluHis: 1.261 ± 0.278
2.077GluIle: 2.077 ± 0.274
3.189GluLys: 3.189 ± 0.442
5.192GluLeu: 5.192 ± 0.842
1.038GluMet: 1.038 ± 0.259
1.854GluAsn: 1.854 ± 0.339
2.151GluPro: 2.151 ± 0.44
3.857GluGln: 3.857 ± 0.461
4.228GluArg: 4.228 ± 0.722
2.893GluSer: 2.893 ± 0.473
3.189GluThr: 3.189 ± 0.395
3.857GluVal: 3.857 ± 0.544
1.261GluTrp: 1.261 ± 0.25
1.78GluTyr: 1.78 ± 0.312
0.0GluXaa: 0.0 ± 0.0
Phe
3.041PheAla: 3.041 ± 0.437
0.074PheCys: 0.074 ± 0.063
3.115PheAsp: 3.115 ± 0.52
1.558PheGlu: 1.558 ± 0.414
1.187PhePhe: 1.187 ± 0.334
2.818PheGly: 2.818 ± 0.468
0.816PheHis: 0.816 ± 0.21
1.632PheIle: 1.632 ± 0.271
1.928PheLys: 1.928 ± 0.352
2.67PheLeu: 2.67 ± 0.438
0.593PheMet: 0.593 ± 0.202
1.928PheAsn: 1.928 ± 0.319
1.409PhePro: 1.409 ± 0.52
1.409PheGln: 1.409 ± 0.269
2.225PheArg: 2.225 ± 0.366
1.632PheSer: 1.632 ± 0.464
1.854PheThr: 1.854 ± 0.345
2.077PheVal: 2.077 ± 0.393
0.593PheTrp: 0.593 ± 0.173
0.816PheTyr: 0.816 ± 0.262
0.0PheXaa: 0.0 ± 0.0
Gly
7.194GlyAla: 7.194 ± 0.883
0.445GlyCys: 0.445 ± 0.239
4.673GlyAsp: 4.673 ± 0.585
3.708GlyGlu: 3.708 ± 0.561
2.818GlyPhe: 2.818 ± 0.499
7.639GlyGly: 7.639 ± 1.029
1.706GlyHis: 1.706 ± 0.355
3.115GlyIle: 3.115 ± 0.436
5.34GlyLys: 5.34 ± 0.525
6.008GlyLeu: 6.008 ± 0.69
2.522GlyMet: 2.522 ± 0.431
3.189GlyAsn: 3.189 ± 0.627
2.67GlyPro: 2.67 ± 0.539
2.448GlyGln: 2.448 ± 0.435
4.821GlyArg: 4.821 ± 0.64
4.376GlySer: 4.376 ± 0.56
7.046GlyThr: 7.046 ± 0.918
6.527GlyVal: 6.527 ± 0.761
1.409GlyTrp: 1.409 ± 0.346
3.041GlyTyr: 3.041 ± 0.593
0.0GlyXaa: 0.0 ± 0.0
His
2.299HisAla: 2.299 ± 0.328
0.445HisCys: 0.445 ± 0.201
1.483HisAsp: 1.483 ± 0.358
1.483HisGlu: 1.483 ± 0.259
0.742HisPhe: 0.742 ± 0.235
1.038HisGly: 1.038 ± 0.249
0.593HisHis: 0.593 ± 0.225
1.187HisIle: 1.187 ± 0.273
1.038HisLys: 1.038 ± 0.274
1.632HisLeu: 1.632 ± 0.445
0.742HisMet: 0.742 ± 0.198
0.445HisAsn: 0.445 ± 0.204
0.964HisPro: 0.964 ± 0.303
0.89HisGln: 0.89 ± 0.317
0.964HisArg: 0.964 ± 0.221
1.038HisSer: 1.038 ± 0.263
1.335HisThr: 1.335 ± 0.339
1.558HisVal: 1.558 ± 0.353
0.371HisTrp: 0.371 ± 0.153
0.668HisTyr: 0.668 ± 0.2
0.0HisXaa: 0.0 ± 0.0
Ile
5.043IleAla: 5.043 ± 0.575
0.742IleCys: 0.742 ± 0.247
2.893IleAsp: 2.893 ± 0.435
3.189IleGlu: 3.189 ± 0.595
0.89IlePhe: 0.89 ± 0.237
3.189IleGly: 3.189 ± 0.604
1.261IleHis: 1.261 ± 0.24
2.077IleIle: 2.077 ± 0.342
2.151IleLys: 2.151 ± 0.393
2.67IleLeu: 2.67 ± 0.568
0.668IleMet: 0.668 ± 0.242
2.151IleAsn: 2.151 ± 0.47
1.706IlePro: 1.706 ± 0.371
2.003IleGln: 2.003 ± 0.429
3.338IleArg: 3.338 ± 0.642
2.225IleSer: 2.225 ± 0.408
2.596IleThr: 2.596 ± 0.531
3.115IleVal: 3.115 ± 0.53
0.297IleTrp: 0.297 ± 0.145
0.89IleTyr: 0.89 ± 0.242
0.0IleXaa: 0.0 ± 0.0
Lys
5.34LysAla: 5.34 ± 0.657
0.297LysCys: 0.297 ± 0.161
2.299LysAsp: 2.299 ± 0.358
3.189LysGlu: 3.189 ± 0.471
1.409LysPhe: 1.409 ± 0.328
4.079LysGly: 4.079 ± 0.668
0.816LysHis: 0.816 ± 0.202
2.003LysIle: 2.003 ± 0.35
2.225LysLys: 2.225 ± 0.381
5.414LysLeu: 5.414 ± 0.487
0.816LysMet: 0.816 ± 0.266
2.225LysAsn: 2.225 ± 0.428
1.409LysPro: 1.409 ± 0.393
1.854LysGln: 1.854 ± 0.312
3.486LysArg: 3.486 ± 0.537
2.744LysSer: 2.744 ± 0.375
2.373LysThr: 2.373 ± 0.472
3.486LysVal: 3.486 ± 0.475
0.742LysTrp: 0.742 ± 0.192
1.928LysTyr: 1.928 ± 0.352
0.0LysXaa: 0.0 ± 0.0
Leu
10.903LeuAla: 10.903 ± 0.86
0.89LeuCys: 0.89 ± 0.292
5.637LeuAsp: 5.637 ± 0.74
4.376LeuGlu: 4.376 ± 0.67
2.448LeuPhe: 2.448 ± 0.442
6.601LeuGly: 6.601 ± 0.953
2.077LeuHis: 2.077 ± 0.32
3.634LeuIle: 3.634 ± 0.572
3.486LeuLys: 3.486 ± 0.461
6.156LeuLeu: 6.156 ± 0.9
2.744LeuMet: 2.744 ± 0.405
3.412LeuAsn: 3.412 ± 0.598
3.486LeuPro: 3.486 ± 0.45
3.708LeuGln: 3.708 ± 0.598
6.898LeuArg: 6.898 ± 0.955
4.45LeuSer: 4.45 ± 0.446
4.45LeuThr: 4.45 ± 0.476
5.488LeuVal: 5.488 ± 0.724
1.483LeuTrp: 1.483 ± 0.372
2.151LeuTyr: 2.151 ± 0.383
0.0LeuXaa: 0.0 ± 0.0
Met
3.189MetAla: 3.189 ± 0.347
0.0MetCys: 0.0 ± 0.0
1.335MetAsp: 1.335 ± 0.289
1.187MetGlu: 1.187 ± 0.384
0.593MetPhe: 0.593 ± 0.18
1.706MetGly: 1.706 ± 0.417
0.297MetHis: 0.297 ± 0.144
0.668MetIle: 0.668 ± 0.182
1.113MetLys: 1.113 ± 0.248
3.115MetLeu: 3.115 ± 0.438
0.668MetMet: 0.668 ± 0.308
0.964MetAsn: 0.964 ± 0.243
0.816MetPro: 0.816 ± 0.242
1.335MetGln: 1.335 ± 0.353
2.522MetArg: 2.522 ± 0.399
1.854MetSer: 1.854 ± 0.271
2.225MetThr: 2.225 ± 0.475
0.816MetVal: 0.816 ± 0.345
0.297MetTrp: 0.297 ± 0.145
0.668MetTyr: 0.668 ± 0.203
0.0MetXaa: 0.0 ± 0.0
Asn
4.969AsnAla: 4.969 ± 0.913
0.445AsnCys: 0.445 ± 0.26
2.67AsnAsp: 2.67 ± 0.59
2.077AsnGlu: 2.077 ± 0.375
1.335AsnPhe: 1.335 ± 0.319
4.376AsnGly: 4.376 ± 0.532
0.593AsnHis: 0.593 ± 0.239
1.409AsnIle: 1.409 ± 0.276
1.409AsnLys: 1.409 ± 0.349
2.967AsnLeu: 2.967 ± 0.486
0.742AsnMet: 0.742 ± 0.218
2.299AsnAsn: 2.299 ± 0.578
2.67AsnPro: 2.67 ± 0.351
1.113AsnGln: 1.113 ± 0.244
2.151AsnArg: 2.151 ± 0.467
2.522AsnSer: 2.522 ± 0.554
2.373AsnThr: 2.373 ± 0.63
2.744AsnVal: 2.744 ± 0.43
0.668AsnTrp: 0.668 ± 0.198
0.89AsnTyr: 0.89 ± 0.262
0.0AsnXaa: 0.0 ± 0.0
Pro
5.563ProAla: 5.563 ± 0.716
0.223ProCys: 0.223 ± 0.111
2.299ProAsp: 2.299 ± 0.387
2.077ProGlu: 2.077 ± 0.372
1.261ProPhe: 1.261 ± 0.29
4.228ProGly: 4.228 ± 0.5
0.593ProHis: 0.593 ± 0.199
1.335ProIle: 1.335 ± 0.275
2.596ProLys: 2.596 ± 0.506
3.041ProLeu: 3.041 ± 0.446
1.335ProMet: 1.335 ± 0.243
2.003ProAsn: 2.003 ± 0.43
1.78ProPro: 1.78 ± 0.457
1.335ProGln: 1.335 ± 0.328
1.706ProArg: 1.706 ± 0.44
3.486ProSer: 3.486 ± 0.517
2.893ProThr: 2.893 ± 0.461
2.818ProVal: 2.818 ± 0.386
0.816ProTrp: 0.816 ± 0.279
1.78ProTyr: 1.78 ± 0.404
0.0ProXaa: 0.0 ± 0.0
Gln
6.675GlnAla: 6.675 ± 0.722
0.223GlnCys: 0.223 ± 0.135
1.928GlnAsp: 1.928 ± 0.344
2.151GlnGlu: 2.151 ± 0.37
1.928GlnPhe: 1.928 ± 0.421
3.189GlnGly: 3.189 ± 0.563
1.409GlnHis: 1.409 ± 0.345
1.187GlnIle: 1.187 ± 0.318
2.003GlnLys: 2.003 ± 0.305
2.522GlnLeu: 2.522 ± 0.488
1.113GlnMet: 1.113 ± 0.331
0.89GlnAsn: 0.89 ± 0.24
2.151GlnPro: 2.151 ± 0.415
3.338GlnGln: 3.338 ± 0.866
3.041GlnArg: 3.041 ± 0.414
2.67GlnSer: 2.67 ± 0.375
2.448GlnThr: 2.448 ± 0.438
2.522GlnVal: 2.522 ± 0.498
0.89GlnTrp: 0.89 ± 0.266
0.964GlnTyr: 0.964 ± 0.312
0.0GlnXaa: 0.0 ± 0.0
Arg
6.675ArgAla: 6.675 ± 0.707
0.816ArgCys: 0.816 ± 0.295
3.338ArgAsp: 3.338 ± 0.721
4.005ArgGlu: 4.005 ± 0.572
2.299ArgPhe: 2.299 ± 0.431
4.673ArgGly: 4.673 ± 0.566
1.706ArgHis: 1.706 ± 0.421
3.708ArgIle: 3.708 ± 0.693
2.67ArgLys: 2.67 ± 0.437
6.156ArgLeu: 6.156 ± 0.646
1.928ArgMet: 1.928 ± 0.311
2.744ArgAsn: 2.744 ± 0.378
2.448ArgPro: 2.448 ± 0.355
1.632ArgGln: 1.632 ± 0.348
5.266ArgArg: 5.266 ± 0.753
4.302ArgSer: 4.302 ± 0.696
3.412ArgThr: 3.412 ± 0.456
4.153ArgVal: 4.153 ± 0.64
1.483ArgTrp: 1.483 ± 0.356
2.003ArgTyr: 2.003 ± 0.405
0.0ArgXaa: 0.0 ± 0.0
Ser
5.563SerAla: 5.563 ± 0.546
0.445SerCys: 0.445 ± 0.19
2.967SerAsp: 2.967 ± 0.471
2.744SerGlu: 2.744 ± 0.346
2.151SerPhe: 2.151 ± 0.388
6.082SerGly: 6.082 ± 0.858
1.038SerHis: 1.038 ± 0.223
2.744SerIle: 2.744 ± 0.421
3.263SerLys: 3.263 ± 0.579
5.563SerLeu: 5.563 ± 0.659
1.483SerMet: 1.483 ± 0.292
2.003SerAsn: 2.003 ± 0.466
2.596SerPro: 2.596 ± 0.33
2.373SerGln: 2.373 ± 0.408
3.115SerArg: 3.115 ± 0.554
3.486SerSer: 3.486 ± 0.542
4.005SerThr: 4.005 ± 0.617
3.486SerVal: 3.486 ± 0.513
0.593SerTrp: 0.593 ± 0.255
1.558SerTyr: 1.558 ± 0.352
0.0SerXaa: 0.0 ± 0.0
Thr
6.527ThrAla: 6.527 ± 1.327
0.371ThrCys: 0.371 ± 0.158
2.967ThrAsp: 2.967 ± 0.495
4.079ThrGlu: 4.079 ± 0.462
2.299ThrPhe: 2.299 ± 0.274
6.156ThrGly: 6.156 ± 0.543
1.187ThrHis: 1.187 ± 0.262
2.744ThrIle: 2.744 ± 0.517
2.744ThrLys: 2.744 ± 0.615
5.711ThrLeu: 5.711 ± 0.602
0.816ThrMet: 0.816 ± 0.198
1.558ThrAsn: 1.558 ± 0.499
3.634ThrPro: 3.634 ± 0.513
2.077ThrGln: 2.077 ± 0.357
3.56ThrArg: 3.56 ± 0.628
3.041ThrSer: 3.041 ± 0.487
5.043ThrThr: 5.043 ± 0.598
3.931ThrVal: 3.931 ± 0.629
0.593ThrTrp: 0.593 ± 0.186
2.151ThrTyr: 2.151 ± 0.38
0.0ThrXaa: 0.0 ± 0.0
Val
7.268ValAla: 7.268 ± 0.901
0.445ValCys: 0.445 ± 0.183
3.708ValAsp: 3.708 ± 0.475
3.486ValGlu: 3.486 ± 0.662
1.854ValPhe: 1.854 ± 0.422
6.156ValGly: 6.156 ± 0.834
1.706ValHis: 1.706 ± 0.324
3.189ValIle: 3.189 ± 0.5
3.486ValLys: 3.486 ± 0.462
4.969ValLeu: 4.969 ± 0.559
1.113ValMet: 1.113 ± 0.273
3.115ValAsn: 3.115 ± 0.512
3.115ValPro: 3.115 ± 0.4
2.67ValGln: 2.67 ± 0.495
3.783ValArg: 3.783 ± 0.49
4.302ValSer: 4.302 ± 0.731
3.857ValThr: 3.857 ± 0.572
4.45ValVal: 4.45 ± 0.586
1.409ValTrp: 1.409 ± 0.336
2.818ValTyr: 2.818 ± 0.565
0.0ValXaa: 0.0 ± 0.0
Trp
1.706TrpAla: 1.706 ± 0.373
0.148TrpCys: 0.148 ± 0.115
0.964TrpAsp: 0.964 ± 0.372
1.261TrpGlu: 1.261 ± 0.386
1.038TrpPhe: 1.038 ± 0.275
0.668TrpGly: 0.668 ± 0.195
0.223TrpHis: 0.223 ± 0.14
0.445TrpIle: 0.445 ± 0.162
0.445TrpLys: 0.445 ± 0.189
1.706TrpLeu: 1.706 ± 0.506
0.519TrpMet: 0.519 ± 0.182
0.964TrpAsn: 0.964 ± 0.296
0.297TrpPro: 0.297 ± 0.141
0.964TrpGln: 0.964 ± 0.23
0.816TrpArg: 0.816 ± 0.275
0.89TrpSer: 0.89 ± 0.243
0.89TrpThr: 0.89 ± 0.253
1.409TrpVal: 1.409 ± 0.323
0.297TrpTrp: 0.297 ± 0.131
0.668TrpTyr: 0.668 ± 0.203
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.373TyrAla: 2.373 ± 0.603
0.297TyrCys: 0.297 ± 0.153
1.409TyrAsp: 1.409 ± 0.327
1.854TyrGlu: 1.854 ± 0.388
1.483TyrPhe: 1.483 ± 0.378
2.67TyrGly: 2.67 ± 0.365
0.593TyrHis: 0.593 ± 0.175
1.038TyrIle: 1.038 ± 0.305
1.483TyrLys: 1.483 ± 0.282
2.596TyrLeu: 2.596 ± 0.406
0.371TyrMet: 0.371 ± 0.171
1.113TyrAsn: 1.113 ± 0.278
1.483TyrPro: 1.483 ± 0.373
1.706TyrGln: 1.706 ± 0.337
2.151TyrArg: 2.151 ± 0.461
2.373TyrSer: 2.373 ± 0.535
2.299TyrThr: 2.299 ± 0.402
2.522TyrVal: 2.522 ± 0.386
0.297TyrTrp: 0.297 ± 0.175
0.371TyrTyr: 0.371 ± 0.184
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 53 proteins (13484 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski