Eimeria maxima (Coccidian parasite)

Taxonomy: cellular organisms; Eukaryota; Sar; Alveolata; Apicomplexa; Conoidasida; Coccidia; Eucoccidiorida; Eimeriorina; Eimeriidae; Eimeria

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6057 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|U6M8M7|U6M8M7_EIMMA Ectonucleoside triphosphate diphosphohydrolase putative OS=Eimeria maxima OX=5804 GN=EMWEY_00006300 PE=4 SV=1
MM1 pKa = 7.88GDD3 pKa = 3.49GLEE6 pKa = 4.05QQEE9 pKa = 5.35DD10 pKa = 3.71KK11 pKa = 11.05QQQQQQQQQQQQQQQQQQQQQQQQQQQHH39 pKa = 6.91DD40 pKa = 4.07DD41 pKa = 3.68DD42 pKa = 6.24DD43 pKa = 4.42FVLDD47 pKa = 4.78LDD49 pKa = 5.04EE50 pKa = 6.59DD51 pKa = 3.87EE52 pKa = 4.95QDD54 pKa = 3.7NSKK57 pKa = 9.51QQQQQQQQQQQQQQQQEE74 pKa = 4.28QQQEE78 pKa = 4.25CSNNGSICVSDD89 pKa = 4.34AEE91 pKa = 4.47SSVAASAAPASSAATAAAAATAAAAAAWDD120 pKa = 4.04RR121 pKa = 11.84EE122 pKa = 4.32SQEE125 pKa = 4.05EE126 pKa = 4.51GLVDD130 pKa = 4.15MWNMLHH136 pKa = 7.48PDD138 pKa = 4.34GPQQQQQQQQQQQQQQQQQQQQQGSEE164 pKa = 4.43KK165 pKa = 10.47YY166 pKa = 9.33PLSPTDD172 pKa = 3.09TSYY175 pKa = 10.71TYY177 pKa = 10.17PHH179 pKa = 6.78HH180 pKa = 6.31QQQQQQQQQEE190 pKa = 4.28QQQQQQQHH198 pKa = 4.78QSHH201 pKa = 6.46NNDD204 pKa = 2.65IPFDD208 pKa = 3.94FFNINLFNPLPAAMVSNNN226 pKa = 3.11

Molecular weight: 26.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|U6M688|U6M688_EIMMA Uncharacterized protein OS=Eimeria maxima OX=5804 GN=EMWEY_00046210 PE=4 SV=1
MM1 pKa = 7.43SRR3 pKa = 11.84LGLSKK8 pKa = 10.9ALLRR12 pKa = 11.84FSLARR17 pKa = 11.84LRR19 pKa = 11.84PAATAAAAAATAATTAPAPSRR40 pKa = 11.84RR41 pKa = 11.84LLSSLSAAAPAAAAAGAAAAPAAAAGTEE69 pKa = 4.17TSPGVRR75 pKa = 11.84PPSLEE80 pKa = 3.74GKK82 pKa = 9.92FFTPAKK88 pKa = 9.09PWGGEE93 pKa = 3.77QQQQQQQQQHH103 pKa = 5.7LQQQQHH109 pKa = 5.59LHH111 pKa = 4.86QQQQHH116 pKa = 5.29LHH118 pKa = 4.74QQQQHH123 pKa = 5.27FHH125 pKa = 5.05QQQHH129 pKa = 5.48LHH131 pKa = 5.15QQQQLHH137 pKa = 5.12QQQQHH142 pKa = 5.13LHH144 pKa = 5.42HH145 pKa = 6.71HH146 pKa = 5.28QQQLHH151 pKa = 5.94HH152 pKa = 5.79QQQLHH157 pKa = 4.55QQQHH161 pKa = 5.23LHH163 pKa = 5.08QQQQLHH169 pKa = 6.12HH170 pKa = 5.63QQQLHH175 pKa = 4.81QQQQHH180 pKa = 5.34LHH182 pKa = 4.7QQQHH186 pKa = 5.41LHH188 pKa = 4.98QQQHH192 pKa = 5.81LQQQLQQQAQQQQQRR207 pKa = 11.84GRR209 pKa = 11.84GAFLPADD216 pKa = 4.31DD217 pKa = 4.56PWYY220 pKa = 9.76SSSYY224 pKa = 10.26EE225 pKa = 4.04PVSPPPRR232 pKa = 11.84QQQQQQQQQQQQQHH246 pKa = 5.16WRR248 pKa = 11.84PSAATAAAGGPGAGLRR264 pKa = 11.84PIDD267 pKa = 3.63WSKK270 pKa = 11.82KK271 pKa = 7.87NLPPIYY277 pKa = 9.33PVAPPPPPAAAAAAAAPGAAAAAAAAAATSAARR310 pKa = 11.84MKK312 pKa = 10.54EE313 pKa = 3.81LGIKK317 pKa = 9.91IEE319 pKa = 4.48GNAPLPLLLPASLPAAFAAAGLPDD343 pKa = 4.77LLLHH347 pKa = 7.04ALQHH351 pKa = 6.73LPQQQQQQQQQQQQQQQQISTLFPIQQIGWSCCFTGRR388 pKa = 11.84DD389 pKa = 4.19LIAIAQTGSGKK400 pKa = 9.22TIGYY404 pKa = 7.78LIPALIHH411 pKa = 6.94LLRR414 pKa = 11.84QQQQQQLQQQQQQQQGNSSSSSSSSSSRR442 pKa = 11.84GPTVLIVGPTRR453 pKa = 11.84EE454 pKa = 4.32LVQQIHH460 pKa = 6.5AEE462 pKa = 4.06CQRR465 pKa = 11.84ILKK468 pKa = 9.82AAAAAAAARR477 pKa = 11.84ASAAAAGGTATAAAAAAAAAAANTPLARR505 pKa = 11.84CVAVYY510 pKa = 10.29GGASRR515 pKa = 11.84QQQILLLRR523 pKa = 11.84SACDD527 pKa = 3.61IIVCTPGRR535 pKa = 3.69

Molecular weight: 57.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6057

0

6057

4025948

9

9976

664.7

71.45

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.095 ± 0.084

1.781 ± 0.02

3.887 ± 0.022

6.32 ± 0.04

2.384 ± 0.017

6.412 ± 0.042

2.17 ± 0.014

2.733 ± 0.019

3.647 ± 0.027

9.215 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.43 ± 0.012

2.65 ± 0.018

5.725 ± 0.035

10.399 ± 0.1

5.638 ± 0.028

9.449 ± 0.049

4.819 ± 0.021

4.808 ± 0.027

0.913 ± 0.008

1.525 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski