Amino acid dipepetide frequency for Enterococcus phage VFW

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.777AlaAla: 4.777 ± 0.606
0.578AlaCys: 0.578 ± 0.132
3.351AlaAsp: 3.351 ± 0.341
4.392AlaGlu: 4.392 ± 0.504
2.62AlaPhe: 2.62 ± 0.333
3.775AlaGly: 3.775 ± 0.468
1.002AlaHis: 1.002 ± 0.2
4.237AlaIle: 4.237 ± 0.43
5.123AlaLys: 5.123 ± 0.551
4.892AlaLeu: 4.892 ± 0.487
1.656AlaMet: 1.656 ± 0.244
3.544AlaAsn: 3.544 ± 0.424
1.695AlaPro: 1.695 ± 0.316
2.62AlaGln: 2.62 ± 0.318
2.273AlaArg: 2.273 ± 0.292
2.774AlaSer: 2.774 ± 0.462
3.159AlaThr: 3.159 ± 0.395
2.658AlaVal: 2.658 ± 0.419
0.655AlaTrp: 0.655 ± 0.195
2.658AlaTyr: 2.658 ± 0.304
0.0AlaXaa: 0.0 ± 0.0
Cys
0.231CysAla: 0.231 ± 0.093
0.039CysCys: 0.039 ± 0.041
0.539CysAsp: 0.539 ± 0.14
0.616CysGlu: 0.616 ± 0.128
0.347CysPhe: 0.347 ± 0.109
0.424CysGly: 0.424 ± 0.139
0.154CysHis: 0.154 ± 0.102
0.385CysIle: 0.385 ± 0.121
0.693CysLys: 0.693 ± 0.172
0.732CysLeu: 0.732 ± 0.17
0.039CysMet: 0.039 ± 0.037
0.231CysAsn: 0.231 ± 0.096
0.231CysPro: 0.231 ± 0.097
0.27CysGln: 0.27 ± 0.123
0.424CysArg: 0.424 ± 0.126
0.193CysSer: 0.193 ± 0.104
0.193CysThr: 0.193 ± 0.088
0.616CysVal: 0.616 ± 0.138
0.193CysTrp: 0.193 ± 0.091
0.27CysTyr: 0.27 ± 0.104
0.0CysXaa: 0.0 ± 0.0
Asp
3.66AspAla: 3.66 ± 0.359
0.347AspCys: 0.347 ± 0.117
3.66AspAsp: 3.66 ± 0.506
6.703AspGlu: 6.703 ± 0.427
2.928AspPhe: 2.928 ± 0.427
4.931AspGly: 4.931 ± 0.446
0.925AspHis: 0.925 ± 0.203
4.969AspIle: 4.969 ± 0.459
5.547AspLys: 5.547 ± 0.445
5.663AspLeu: 5.663 ± 0.449
2.003AspMet: 2.003 ± 0.312
4.122AspAsn: 4.122 ± 0.514
1.425AspPro: 1.425 ± 0.287
1.695AspGln: 1.695 ± 0.276
2.851AspArg: 2.851 ± 0.33
3.236AspSer: 3.236 ± 0.343
2.62AspThr: 2.62 ± 0.417
3.814AspVal: 3.814 ± 0.421
1.002AspTrp: 1.002 ± 0.199
3.005AspTyr: 3.005 ± 0.453
0.0AspXaa: 0.0 ± 0.0
Glu
4.738GluAla: 4.738 ± 0.463
0.385GluCys: 0.385 ± 0.113
5.393GluAsp: 5.393 ± 0.455
7.204GluGlu: 7.204 ± 0.568
3.082GluPhe: 3.082 ± 0.305
3.506GluGly: 3.506 ± 0.349
0.847GluHis: 0.847 ± 0.18
7.743GluIle: 7.743 ± 0.616
10.17GluLys: 10.17 ± 0.658
8.552GluLeu: 8.552 ± 0.681
2.735GluMet: 2.735 ± 0.304
6.472GluAsn: 6.472 ± 0.561
1.656GluPro: 1.656 ± 0.253
3.467GluGln: 3.467 ± 0.455
2.735GluArg: 2.735 ± 0.32
3.506GluSer: 3.506 ± 0.325
3.814GluThr: 3.814 ± 0.393
5.509GluVal: 5.509 ± 0.569
0.77GluTrp: 0.77 ± 0.205
3.12GluTyr: 3.12 ± 0.4
0.0GluXaa: 0.0 ± 0.0
Phe
2.35PheAla: 2.35 ± 0.236
0.424PheCys: 0.424 ± 0.156
3.159PheAsp: 3.159 ± 0.353
3.775PheGlu: 3.775 ± 0.409
2.08PhePhe: 2.08 ± 0.355
3.043PheGly: 3.043 ± 0.372
0.809PheHis: 0.809 ± 0.141
2.774PheIle: 2.774 ± 0.304
3.583PheLys: 3.583 ± 0.351
3.737PheLeu: 3.737 ± 0.496
1.425PheMet: 1.425 ± 0.281
3.082PheAsn: 3.082 ± 0.322
1.348PhePro: 1.348 ± 0.251
1.541PheGln: 1.541 ± 0.219
1.541PheArg: 1.541 ± 0.228
2.62PheSer: 2.62 ± 0.342
2.465PheThr: 2.465 ± 0.365
2.35PheVal: 2.35 ± 0.307
0.385PheTrp: 0.385 ± 0.108
1.233PheTyr: 1.233 ± 0.251
0.0PheXaa: 0.0 ± 0.0
Gly
3.39GlyAla: 3.39 ± 0.424
0.385GlyCys: 0.385 ± 0.12
3.159GlyAsp: 3.159 ± 0.361
4.584GlyGlu: 4.584 ± 0.547
2.504GlyPhe: 2.504 ± 0.305
2.928GlyGly: 2.928 ± 0.339
1.156GlyHis: 1.156 ± 0.263
4.969GlyIle: 4.969 ± 0.602
6.087GlyLys: 6.087 ± 0.391
5.162GlyLeu: 5.162 ± 0.459
1.579GlyMet: 1.579 ± 0.257
3.274GlyAsn: 3.274 ± 0.344
0.809GlyPro: 0.809 ± 0.165
2.042GlyGln: 2.042 ± 0.242
2.196GlyArg: 2.196 ± 0.299
3.005GlySer: 3.005 ± 0.343
3.737GlyThr: 3.737 ± 0.441
3.929GlyVal: 3.929 ± 0.386
0.693GlyTrp: 0.693 ± 0.183
2.697GlyTyr: 2.697 ± 0.323
0.0GlyXaa: 0.0 ± 0.0
His
0.578HisAla: 0.578 ± 0.128
0.154HisCys: 0.154 ± 0.086
0.963HisAsp: 0.963 ± 0.228
1.079HisGlu: 1.079 ± 0.192
0.963HisPhe: 0.963 ± 0.197
1.425HisGly: 1.425 ± 0.358
0.308HisHis: 0.308 ± 0.123
1.464HisIle: 1.464 ± 0.222
1.194HisLys: 1.194 ± 0.18
1.233HisLeu: 1.233 ± 0.248
0.424HisMet: 0.424 ± 0.127
1.002HisAsn: 1.002 ± 0.206
0.231HisPro: 0.231 ± 0.078
0.231HisGln: 0.231 ± 0.079
0.385HisArg: 0.385 ± 0.101
0.693HisSer: 0.693 ± 0.19
0.424HisThr: 0.424 ± 0.138
1.271HisVal: 1.271 ± 0.244
0.154HisTrp: 0.154 ± 0.074
0.77HisTyr: 0.77 ± 0.166
0.0HisXaa: 0.0 ± 0.0
Ile
4.623IleAla: 4.623 ± 0.547
0.693IleCys: 0.693 ± 0.196
6.895IleAsp: 6.895 ± 0.459
7.782IleGlu: 7.782 ± 0.749
3.197IlePhe: 3.197 ± 0.424
4.777IleGly: 4.777 ± 0.528
1.079IleHis: 1.079 ± 0.177
5.393IleIle: 5.393 ± 0.45
6.741IleLys: 6.741 ± 0.594
5.778IleLeu: 5.778 ± 0.573
2.157IleMet: 2.157 ± 0.277
5.046IleAsn: 5.046 ± 0.386
2.273IlePro: 2.273 ± 0.326
1.541IleGln: 1.541 ± 0.278
2.966IleArg: 2.966 ± 0.329
3.583IleSer: 3.583 ± 0.452
4.237IleThr: 4.237 ± 0.427
5.046IleVal: 5.046 ± 0.45
0.963IleTrp: 0.963 ± 0.433
2.774IleTyr: 2.774 ± 0.307
0.0IleXaa: 0.0 ± 0.0
Lys
5.316LysAla: 5.316 ± 0.603
0.308LysCys: 0.308 ± 0.104
5.663LysAsp: 5.663 ± 0.454
8.706LysGlu: 8.706 ± 0.652
3.428LysPhe: 3.428 ± 0.366
4.353LysGly: 4.353 ± 0.472
1.502LysHis: 1.502 ± 0.208
7.473LysIle: 7.473 ± 0.538
8.937LysLys: 8.937 ± 0.61
7.319LysLeu: 7.319 ± 0.668
3.814LysMet: 3.814 ± 0.401
6.626LysAsn: 6.626 ± 0.563
1.464LysPro: 1.464 ± 0.263
3.197LysGln: 3.197 ± 0.406
2.658LysArg: 2.658 ± 0.323
4.122LysSer: 4.122 ± 0.531
4.661LysThr: 4.661 ± 0.57
6.549LysVal: 6.549 ± 0.413
1.348LysTrp: 1.348 ± 0.206
5.278LysTyr: 5.278 ± 0.598
0.0LysXaa: 0.0 ± 0.0
Leu
5.47LeuAla: 5.47 ± 0.523
0.693LeuCys: 0.693 ± 0.189
5.432LeuAsp: 5.432 ± 0.39
7.242LeuGlu: 7.242 ± 0.533
3.621LeuPhe: 3.621 ± 0.408
4.045LeuGly: 4.045 ± 0.522
1.271LeuHis: 1.271 ± 0.237
6.279LeuIle: 6.279 ± 0.445
7.743LeuLys: 7.743 ± 0.475
6.51LeuLeu: 6.51 ± 0.484
2.311LeuMet: 2.311 ± 0.315
6.356LeuAsn: 6.356 ± 0.521
2.119LeuPro: 2.119 ± 0.307
3.66LeuGln: 3.66 ± 0.466
2.735LeuArg: 2.735 ± 0.39
4.584LeuSer: 4.584 ± 0.379
4.43LeuThr: 4.43 ± 0.443
4.815LeuVal: 4.815 ± 0.551
1.002LeuTrp: 1.002 ± 0.241
3.621LeuTyr: 3.621 ± 0.439
0.0LeuXaa: 0.0 ± 0.0
Met
1.965MetAla: 1.965 ± 0.261
0.116MetCys: 0.116 ± 0.061
2.35MetAsp: 2.35 ± 0.317
3.351MetGlu: 3.351 ± 0.36
0.847MetPhe: 0.847 ± 0.171
1.117MetGly: 1.117 ± 0.217
0.308MetHis: 0.308 ± 0.118
2.465MetIle: 2.465 ± 0.284
3.737MetLys: 3.737 ± 0.377
2.427MetLeu: 2.427 ± 0.245
0.925MetMet: 0.925 ± 0.202
1.811MetAsn: 1.811 ± 0.268
0.693MetPro: 0.693 ± 0.145
0.963MetGln: 0.963 ± 0.201
1.04MetArg: 1.04 ± 0.251
1.464MetSer: 1.464 ± 0.216
1.965MetThr: 1.965 ± 0.283
1.579MetVal: 1.579 ± 0.276
0.385MetTrp: 0.385 ± 0.115
0.963MetTyr: 0.963 ± 0.178
0.0MetXaa: 0.0 ± 0.0
Asn
3.351AsnAla: 3.351 ± 0.384
0.308AsnCys: 0.308 ± 0.112
4.738AsnAsp: 4.738 ± 0.469
5.239AsnGlu: 5.239 ± 0.491
3.005AsnPhe: 3.005 ± 0.314
5.624AsnGly: 5.624 ± 0.396
0.77AsnHis: 0.77 ± 0.167
5.046AsnIle: 5.046 ± 0.419
6.895AsnLys: 6.895 ± 0.528
4.815AsnLeu: 4.815 ± 0.45
1.618AsnMet: 1.618 ± 0.249
4.353AsnAsn: 4.353 ± 0.513
2.003AsnPro: 2.003 ± 0.28
1.888AsnGln: 1.888 ± 0.258
2.388AsnArg: 2.388 ± 0.306
3.351AsnSer: 3.351 ± 0.359
2.928AsnThr: 2.928 ± 0.311
4.045AsnVal: 4.045 ± 0.454
0.732AsnTrp: 0.732 ± 0.161
2.658AsnTyr: 2.658 ± 0.415
0.0AsnXaa: 0.0 ± 0.0
Pro
1.425ProAla: 1.425 ± 0.367
0.116ProCys: 0.116 ± 0.066
1.734ProAsp: 1.734 ± 0.273
1.502ProGlu: 1.502 ± 0.3
1.425ProPhe: 1.425 ± 0.224
1.233ProGly: 1.233 ± 0.198
0.385ProHis: 0.385 ± 0.117
2.311ProIle: 2.311 ± 0.274
2.042ProLys: 2.042 ± 0.298
2.003ProLeu: 2.003 ± 0.258
0.732ProMet: 0.732 ± 0.167
1.656ProAsn: 1.656 ± 0.279
0.385ProPro: 0.385 ± 0.148
0.925ProGln: 0.925 ± 0.224
0.963ProArg: 0.963 ± 0.217
1.502ProSer: 1.502 ± 0.215
1.464ProThr: 1.464 ± 0.225
1.695ProVal: 1.695 ± 0.257
0.462ProTrp: 0.462 ± 0.163
1.31ProTyr: 1.31 ± 0.233
0.0ProXaa: 0.0 ± 0.0
Gln
2.119GlnAla: 2.119 ± 0.409
0.424GlnCys: 0.424 ± 0.124
1.387GlnAsp: 1.387 ± 0.293
2.966GlnGlu: 2.966 ± 0.455
1.579GlnPhe: 1.579 ± 0.252
1.156GlnGly: 1.156 ± 0.191
0.501GlnHis: 0.501 ± 0.154
2.928GlnIle: 2.928 ± 0.298
2.889GlnLys: 2.889 ± 0.303
3.929GlnLeu: 3.929 ± 0.429
1.04GlnMet: 1.04 ± 0.228
1.772GlnAsn: 1.772 ± 0.277
1.348GlnPro: 1.348 ± 0.206
1.811GlnGln: 1.811 ± 0.451
0.963GlnArg: 0.963 ± 0.171
1.656GlnSer: 1.656 ± 0.276
2.119GlnThr: 2.119 ± 0.296
2.234GlnVal: 2.234 ± 0.283
0.501GlnTrp: 0.501 ± 0.137
1.656GlnTyr: 1.656 ± 0.251
0.0GlnXaa: 0.0 ± 0.0
Arg
2.311ArgAla: 2.311 ± 0.311
0.308ArgCys: 0.308 ± 0.115
2.234ArgAsp: 2.234 ± 0.327
3.005ArgGlu: 3.005 ± 0.34
1.849ArgPhe: 1.849 ± 0.292
2.196ArgGly: 2.196 ± 0.313
0.385ArgHis: 0.385 ± 0.104
2.697ArgIle: 2.697 ± 0.315
3.313ArgLys: 3.313 ± 0.351
2.966ArgLeu: 2.966 ± 0.349
1.156ArgMet: 1.156 ± 0.268
2.504ArgAsn: 2.504 ± 0.28
0.925ArgPro: 0.925 ± 0.18
1.387ArgGln: 1.387 ± 0.253
1.464ArgArg: 1.464 ± 0.25
1.772ArgSer: 1.772 ± 0.23
1.117ArgThr: 1.117 ± 0.241
2.196ArgVal: 2.196 ± 0.277
0.308ArgTrp: 0.308 ± 0.109
1.579ArgTyr: 1.579 ± 0.243
0.0ArgXaa: 0.0 ± 0.0
Ser
2.889SerAla: 2.889 ± 0.317
0.385SerCys: 0.385 ± 0.125
3.197SerAsp: 3.197 ± 0.377
3.852SerGlu: 3.852 ± 0.426
2.427SerPhe: 2.427 ± 0.277
3.583SerGly: 3.583 ± 0.34
0.847SerHis: 0.847 ± 0.175
3.814SerIle: 3.814 ± 0.401
4.314SerLys: 4.314 ± 0.391
4.469SerLeu: 4.469 ± 0.404
1.734SerMet: 1.734 ± 0.286
3.082SerAsn: 3.082 ± 0.373
1.656SerPro: 1.656 ± 0.238
1.811SerGln: 1.811 ± 0.243
1.618SerArg: 1.618 ± 0.296
3.159SerSer: 3.159 ± 0.543
3.12SerThr: 3.12 ± 0.333
2.658SerVal: 2.658 ± 0.357
0.462SerTrp: 0.462 ± 0.136
2.08SerTyr: 2.08 ± 0.274
0.0SerXaa: 0.0 ± 0.0
Thr
2.851ThrAla: 2.851 ± 0.414
0.231ThrCys: 0.231 ± 0.091
2.658ThrAsp: 2.658 ± 0.256
4.199ThrGlu: 4.199 ± 0.368
2.504ThrPhe: 2.504 ± 0.297
3.506ThrGly: 3.506 ± 0.426
0.847ThrHis: 0.847 ± 0.178
4.661ThrIle: 4.661 ± 0.447
3.544ThrLys: 3.544 ± 0.409
4.738ThrLeu: 4.738 ± 0.393
1.888ThrMet: 1.888 ± 0.299
3.351ThrAsn: 3.351 ± 0.39
1.541ThrPro: 1.541 ± 0.3
1.464ThrGln: 1.464 ± 0.241
1.772ThrArg: 1.772 ± 0.326
2.928ThrSer: 2.928 ± 0.393
3.621ThrThr: 3.621 ± 0.714
4.006ThrVal: 4.006 ± 0.467
0.578ThrTrp: 0.578 ± 0.139
2.042ThrTyr: 2.042 ± 0.28
0.0ThrXaa: 0.0 ± 0.0
Val
3.852ValAla: 3.852 ± 0.388
0.347ValCys: 0.347 ± 0.124
4.584ValAsp: 4.584 ± 0.447
4.738ValGlu: 4.738 ± 0.4
2.658ValPhe: 2.658 ± 0.275
3.005ValGly: 3.005 ± 0.371
0.963ValHis: 0.963 ± 0.163
4.43ValIle: 4.43 ± 0.409
5.894ValLys: 5.894 ± 0.517
4.469ValLeu: 4.469 ± 0.406
1.926ValMet: 1.926 ± 0.269
2.812ValAsn: 2.812 ± 0.35
1.888ValPro: 1.888 ± 0.264
2.35ValGln: 2.35 ± 0.34
2.388ValArg: 2.388 ± 0.289
4.083ValSer: 4.083 ± 0.396
4.122ValThr: 4.122 ± 0.381
3.698ValVal: 3.698 ± 0.5
0.925ValTrp: 0.925 ± 0.274
2.812ValTyr: 2.812 ± 0.351
0.0ValXaa: 0.0 ± 0.0
Trp
0.462TrpAla: 0.462 ± 0.114
0.116TrpCys: 0.116 ± 0.072
0.886TrpAsp: 0.886 ± 0.381
1.156TrpGlu: 1.156 ± 0.282
0.501TrpPhe: 0.501 ± 0.149
0.501TrpGly: 0.501 ± 0.233
0.077TrpHis: 0.077 ± 0.053
0.77TrpIle: 0.77 ± 0.167
0.963TrpLys: 0.963 ± 0.223
1.002TrpLeu: 1.002 ± 0.209
0.27TrpMet: 0.27 ± 0.088
1.541TrpAsn: 1.541 ± 0.528
0.27TrpPro: 0.27 ± 0.109
0.347TrpGln: 0.347 ± 0.124
0.539TrpArg: 0.539 ± 0.124
0.578TrpSer: 0.578 ± 0.164
0.809TrpThr: 0.809 ± 0.158
0.578TrpVal: 0.578 ± 0.147
0.27TrpTrp: 0.27 ± 0.1
0.501TrpTyr: 0.501 ± 0.14
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.08TyrAla: 2.08 ± 0.239
0.462TyrCys: 0.462 ± 0.14
3.159TyrAsp: 3.159 ± 0.374
3.313TyrGlu: 3.313 ± 0.371
2.157TyrPhe: 2.157 ± 0.291
3.159TyrGly: 3.159 ± 0.312
0.809TyrHis: 0.809 ± 0.199
2.735TyrIle: 2.735 ± 0.328
3.313TyrLys: 3.313 ± 0.353
3.544TyrLeu: 3.544 ± 0.462
1.002TyrMet: 1.002 ± 0.209
3.12TyrAsn: 3.12 ± 0.334
1.31TyrPro: 1.31 ± 0.253
1.772TyrGln: 1.772 ± 0.293
1.772TyrArg: 1.772 ± 0.248
2.35TyrSer: 2.35 ± 0.308
1.926TyrThr: 1.926 ± 0.375
2.697TyrVal: 2.697 ± 0.335
0.347TyrTrp: 0.347 ± 0.14
1.734TyrTyr: 1.734 ± 0.253
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 129 proteins (25960 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski