Amino acid dipepetide frequency for Mycobacterium phage Bluefalcon

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
11.761AlaAla: 11.761 ± 1.187
0.316AlaCys: 0.316 ± 0.125
5.754AlaAsp: 5.754 ± 0.683
6.829AlaGlu: 6.829 ± 0.844
2.845AlaPhe: 2.845 ± 0.518
7.019AlaGly: 7.019 ± 0.642
1.328AlaHis: 1.328 ± 0.323
4.932AlaIle: 4.932 ± 0.491
5.438AlaLys: 5.438 ± 0.686
7.588AlaLeu: 7.588 ± 0.816
2.023AlaMet: 2.023 ± 0.402
3.225AlaAsn: 3.225 ± 0.364
4.489AlaPro: 4.489 ± 0.481
3.035AlaGln: 3.035 ± 0.532
5.564AlaArg: 5.564 ± 0.677
4.679AlaSer: 4.679 ± 0.563
5.754AlaThr: 5.754 ± 0.642
7.082AlaVal: 7.082 ± 0.913
1.897AlaTrp: 1.897 ± 0.344
2.023AlaTyr: 2.023 ± 0.355
0.0AlaXaa: 0.0 ± 0.0
Cys
0.569CysAla: 0.569 ± 0.17
0.126CysCys: 0.126 ± 0.095
0.696CysAsp: 0.696 ± 0.196
0.569CysGlu: 0.569 ± 0.203
0.316CysPhe: 0.316 ± 0.132
1.075CysGly: 1.075 ± 0.248
0.126CysHis: 0.126 ± 0.086
0.443CysIle: 0.443 ± 0.173
0.379CysLys: 0.379 ± 0.143
0.822CysLeu: 0.822 ± 0.231
0.126CysMet: 0.126 ± 0.096
0.443CysAsn: 0.443 ± 0.155
0.253CysPro: 0.253 ± 0.11
0.443CysGln: 0.443 ± 0.132
0.506CysArg: 0.506 ± 0.196
0.632CysSer: 0.632 ± 0.218
0.19CysThr: 0.19 ± 0.105
0.506CysVal: 0.506 ± 0.153
0.253CysTrp: 0.253 ± 0.118
0.19CysTyr: 0.19 ± 0.108
0.0CysXaa: 0.0 ± 0.0
Asp
6.323AspAla: 6.323 ± 0.696
1.012AspCys: 1.012 ± 0.246
4.679AspAsp: 4.679 ± 0.536
4.3AspGlu: 4.3 ± 0.617
2.529AspPhe: 2.529 ± 0.449
6.007AspGly: 6.007 ± 0.696
1.391AspHis: 1.391 ± 0.316
2.529AspIle: 2.529 ± 0.386
3.035AspLys: 3.035 ± 0.471
6.892AspLeu: 6.892 ± 0.694
1.77AspMet: 1.77 ± 0.35
1.518AspAsn: 1.518 ± 0.319
4.616AspPro: 4.616 ± 0.699
3.098AspGln: 3.098 ± 0.536
3.478AspArg: 3.478 ± 0.496
2.909AspSer: 2.909 ± 0.419
3.667AspThr: 3.667 ± 0.523
6.133AspVal: 6.133 ± 0.593
1.518AspTrp: 1.518 ± 0.322
3.288AspTyr: 3.288 ± 0.452
0.0AspXaa: 0.0 ± 0.0
Glu
7.461GluAla: 7.461 ± 0.741
0.506GluCys: 0.506 ± 0.212
5.185GluAsp: 5.185 ± 0.629
5.564GluGlu: 5.564 ± 0.89
1.96GluPhe: 1.96 ± 0.315
5.311GluGly: 5.311 ± 0.645
1.644GluHis: 1.644 ± 0.353
4.047GluIle: 4.047 ± 0.539
3.541GluLys: 3.541 ± 0.425
7.461GluLeu: 7.461 ± 0.757
2.087GluMet: 2.087 ± 0.381
2.403GluAsn: 2.403 ± 0.341
2.972GluPro: 2.972 ± 0.498
2.592GluGln: 2.592 ± 0.346
4.932GluArg: 4.932 ± 0.586
2.972GluSer: 2.972 ± 0.383
3.604GluThr: 3.604 ± 0.45
6.197GluVal: 6.197 ± 0.574
1.581GluTrp: 1.581 ± 0.389
2.592GluTyr: 2.592 ± 0.524
0.0GluXaa: 0.0 ± 0.0
Phe
2.972PheAla: 2.972 ± 0.345
0.19PheCys: 0.19 ± 0.102
2.909PheAsp: 2.909 ± 0.384
1.897PheGlu: 1.897 ± 0.319
0.569PhePhe: 0.569 ± 0.214
2.845PheGly: 2.845 ± 0.389
0.632PheHis: 0.632 ± 0.203
1.454PheIle: 1.454 ± 0.28
1.707PheLys: 1.707 ± 0.34
2.719PheLeu: 2.719 ± 0.441
0.822PheMet: 0.822 ± 0.195
1.644PheAsn: 1.644 ± 0.422
1.581PhePro: 1.581 ± 0.28
1.201PheGln: 1.201 ± 0.311
2.087PheArg: 2.087 ± 0.37
2.023PheSer: 2.023 ± 0.394
2.023PheThr: 2.023 ± 0.421
2.34PheVal: 2.34 ± 0.395
0.948PheTrp: 0.948 ± 0.261
1.075PheTyr: 1.075 ± 0.251
0.0PheXaa: 0.0 ± 0.0
Gly
5.501GlyAla: 5.501 ± 0.766
1.012GlyCys: 1.012 ± 0.318
6.007GlyAsp: 6.007 ± 0.765
5.691GlyGlu: 5.691 ± 0.583
2.782GlyPhe: 2.782 ± 0.499
9.864GlyGly: 9.864 ± 2.151
1.897GlyHis: 1.897 ± 0.252
3.92GlyIle: 3.92 ± 0.506
4.616GlyLys: 4.616 ± 0.652
7.208GlyLeu: 7.208 ± 0.832
2.15GlyMet: 2.15 ± 0.335
3.288GlyAsn: 3.288 ± 0.436
3.731GlyPro: 3.731 ± 0.565
2.592GlyGln: 2.592 ± 0.466
4.869GlyArg: 4.869 ± 0.523
4.932GlySer: 4.932 ± 0.806
4.742GlyThr: 4.742 ± 0.766
4.995GlyVal: 4.995 ± 0.669
1.897GlyTrp: 1.897 ± 0.321
2.782GlyTyr: 2.782 ± 0.552
0.0GlyXaa: 0.0 ± 0.0
His
1.454HisAla: 1.454 ± 0.304
0.126HisCys: 0.126 ± 0.093
1.581HisAsp: 1.581 ± 0.321
1.644HisGlu: 1.644 ± 0.373
0.569HisPhe: 0.569 ± 0.15
1.644HisGly: 1.644 ± 0.3
0.506HisHis: 0.506 ± 0.176
1.265HisIle: 1.265 ± 0.294
0.885HisLys: 0.885 ± 0.296
1.581HisLeu: 1.581 ± 0.357
0.126HisMet: 0.126 ± 0.095
0.379HisAsn: 0.379 ± 0.146
0.696HisPro: 0.696 ± 0.238
0.759HisGln: 0.759 ± 0.223
1.518HisArg: 1.518 ± 0.348
0.759HisSer: 0.759 ± 0.206
0.759HisThr: 0.759 ± 0.231
1.707HisVal: 1.707 ± 0.316
0.316HisTrp: 0.316 ± 0.119
0.506HisTyr: 0.506 ± 0.2
0.0HisXaa: 0.0 ± 0.0
Ile
5.501IleAla: 5.501 ± 0.591
0.379IleCys: 0.379 ± 0.144
4.553IleAsp: 4.553 ± 0.462
4.553IleGlu: 4.553 ± 0.564
1.391IlePhe: 1.391 ± 0.42
3.478IleGly: 3.478 ± 0.452
0.822IleHis: 0.822 ± 0.228
1.96IleIle: 1.96 ± 0.367
2.15IleLys: 2.15 ± 0.361
3.414IleLeu: 3.414 ± 0.387
0.759IleMet: 0.759 ± 0.227
2.087IleAsn: 2.087 ± 0.402
3.794IlePro: 3.794 ± 0.424
1.644IleGln: 1.644 ± 0.287
3.225IleArg: 3.225 ± 0.398
2.403IleSer: 2.403 ± 0.416
2.845IleThr: 2.845 ± 0.471
3.035IleVal: 3.035 ± 0.467
0.506IleTrp: 0.506 ± 0.181
1.581IleTyr: 1.581 ± 0.339
0.0IleXaa: 0.0 ± 0.0
Lys
5.817LysAla: 5.817 ± 0.794
0.316LysCys: 0.316 ± 0.13
2.845LysAsp: 2.845 ± 0.423
2.276LysGlu: 2.276 ± 0.464
1.518LysPhe: 1.518 ± 0.406
3.225LysGly: 3.225 ± 0.475
1.012LysHis: 1.012 ± 0.255
2.276LysIle: 2.276 ± 0.513
2.529LysLys: 2.529 ± 0.422
3.478LysLeu: 3.478 ± 0.498
1.138LysMet: 1.138 ± 0.278
1.201LysAsn: 1.201 ± 0.241
1.96LysPro: 1.96 ± 0.409
1.265LysGln: 1.265 ± 0.249
2.529LysArg: 2.529 ± 0.494
2.213LysSer: 2.213 ± 0.39
3.035LysThr: 3.035 ± 0.506
4.173LysVal: 4.173 ± 0.53
0.696LysTrp: 0.696 ± 0.19
1.201LysTyr: 1.201 ± 0.275
0.0LysXaa: 0.0 ± 0.0
Leu
7.841LeuAla: 7.841 ± 0.836
0.696LeuCys: 0.696 ± 0.182
6.133LeuAsp: 6.133 ± 0.699
7.208LeuGlu: 7.208 ± 0.735
1.897LeuPhe: 1.897 ± 0.442
5.754LeuGly: 5.754 ± 0.656
0.885LeuHis: 0.885 ± 0.32
3.794LeuIle: 3.794 ± 0.482
3.92LeuLys: 3.92 ± 0.648
5.691LeuLeu: 5.691 ± 0.612
2.087LeuMet: 2.087 ± 0.361
2.529LeuAsn: 2.529 ± 0.537
4.363LeuPro: 4.363 ± 0.728
2.276LeuGln: 2.276 ± 0.45
5.311LeuArg: 5.311 ± 0.591
5.122LeuSer: 5.122 ± 0.615
5.817LeuThr: 5.817 ± 0.697
5.501LeuVal: 5.501 ± 0.68
1.328LeuTrp: 1.328 ± 0.338
2.719LeuTyr: 2.719 ± 0.448
0.0LeuXaa: 0.0 ± 0.0
Met
1.897MetAla: 1.897 ± 0.444
0.253MetCys: 0.253 ± 0.126
1.201MetAsp: 1.201 ± 0.307
0.885MetGlu: 0.885 ± 0.232
0.759MetPhe: 0.759 ± 0.231
1.518MetGly: 1.518 ± 0.339
0.253MetHis: 0.253 ± 0.136
0.632MetIle: 0.632 ± 0.201
1.328MetLys: 1.328 ± 0.299
2.023MetLeu: 2.023 ± 0.349
0.316MetMet: 0.316 ± 0.156
0.885MetAsn: 0.885 ± 0.25
1.138MetPro: 1.138 ± 0.238
0.569MetGln: 0.569 ± 0.275
1.581MetArg: 1.581 ± 0.383
2.403MetSer: 2.403 ± 0.368
3.098MetThr: 3.098 ± 0.432
1.138MetVal: 1.138 ± 0.312
0.316MetTrp: 0.316 ± 0.122
0.632MetTyr: 0.632 ± 0.247
0.0MetXaa: 0.0 ± 0.0
Asn
2.909AsnAla: 2.909 ± 0.477
0.379AsnCys: 0.379 ± 0.137
2.087AsnAsp: 2.087 ± 0.393
1.707AsnGlu: 1.707 ± 0.314
2.213AsnPhe: 2.213 ± 0.448
3.857AsnGly: 3.857 ± 0.642
0.822AsnHis: 0.822 ± 0.242
1.644AsnIle: 1.644 ± 0.459
0.759AsnLys: 0.759 ± 0.197
2.592AsnLeu: 2.592 ± 0.374
0.632AsnMet: 0.632 ± 0.199
1.075AsnAsn: 1.075 ± 0.257
2.592AsnPro: 2.592 ± 0.547
1.075AsnGln: 1.075 ± 0.293
1.834AsnArg: 1.834 ± 0.328
1.581AsnSer: 1.581 ± 0.286
2.087AsnThr: 2.087 ± 0.411
2.466AsnVal: 2.466 ± 0.347
0.696AsnTrp: 0.696 ± 0.229
0.632AsnTyr: 0.632 ± 0.187
0.0AsnXaa: 0.0 ± 0.0
Pro
4.426ProAla: 4.426 ± 0.657
0.443ProCys: 0.443 ± 0.187
2.972ProAsp: 2.972 ± 0.529
4.363ProGlu: 4.363 ± 0.524
1.581ProPhe: 1.581 ± 0.365
4.236ProGly: 4.236 ± 0.631
0.885ProHis: 0.885 ± 0.229
2.529ProIle: 2.529 ± 0.388
1.454ProLys: 1.454 ± 0.366
3.351ProLeu: 3.351 ± 0.494
0.885ProMet: 0.885 ± 0.225
1.77ProAsn: 1.77 ± 0.362
3.098ProPro: 3.098 ± 0.492
1.834ProGln: 1.834 ± 0.317
2.087ProArg: 2.087 ± 0.377
3.162ProSer: 3.162 ± 0.443
3.857ProThr: 3.857 ± 0.603
4.869ProVal: 4.869 ± 0.613
1.138ProTrp: 1.138 ± 0.369
1.644ProTyr: 1.644 ± 0.355
0.0ProXaa: 0.0 ± 0.0
Gln
3.162GlnAla: 3.162 ± 0.564
0.126GlnCys: 0.126 ± 0.083
1.77GlnAsp: 1.77 ± 0.361
2.656GlnGlu: 2.656 ± 0.358
1.834GlnPhe: 1.834 ± 0.392
2.656GlnGly: 2.656 ± 0.55
1.075GlnHis: 1.075 ± 0.248
2.213GlnIle: 2.213 ± 0.401
1.265GlnLys: 1.265 ± 0.316
3.667GlnLeu: 3.667 ± 0.514
0.569GlnMet: 0.569 ± 0.202
0.885GlnAsn: 0.885 ± 0.2
1.075GlnPro: 1.075 ± 0.274
1.201GlnGln: 1.201 ± 0.324
1.77GlnArg: 1.77 ± 0.323
1.77GlnSer: 1.77 ± 0.363
1.454GlnThr: 1.454 ± 0.262
2.909GlnVal: 2.909 ± 0.393
0.632GlnTrp: 0.632 ± 0.199
1.138GlnTyr: 1.138 ± 0.252
0.0GlnXaa: 0.0 ± 0.0
Arg
5.501ArgAla: 5.501 ± 0.664
0.569ArgCys: 0.569 ± 0.236
3.414ArgAsp: 3.414 ± 0.4
5.122ArgGlu: 5.122 ± 0.674
3.288ArgPhe: 3.288 ± 0.428
4.426ArgGly: 4.426 ± 0.581
1.265ArgHis: 1.265 ± 0.313
3.288ArgIle: 3.288 ± 0.487
2.909ArgLys: 2.909 ± 0.418
5.438ArgLeu: 5.438 ± 0.515
1.454ArgMet: 1.454 ± 0.276
1.96ArgAsn: 1.96 ± 0.37
2.592ArgPro: 2.592 ± 0.429
2.087ArgGln: 2.087 ± 0.368
4.869ArgArg: 4.869 ± 0.566
2.719ArgSer: 2.719 ± 0.435
3.225ArgThr: 3.225 ± 0.525
3.351ArgVal: 3.351 ± 0.478
1.454ArgTrp: 1.454 ± 0.348
1.518ArgTyr: 1.518 ± 0.28
0.0ArgXaa: 0.0 ± 0.0
Ser
3.794SerAla: 3.794 ± 0.468
0.443SerCys: 0.443 ± 0.158
4.489SerAsp: 4.489 ± 0.488
4.679SerGlu: 4.679 ± 0.613
2.466SerPhe: 2.466 ± 0.4
5.501SerGly: 5.501 ± 0.697
0.632SerHis: 0.632 ± 0.21
2.34SerIle: 2.34 ± 0.375
2.023SerLys: 2.023 ± 0.436
2.972SerLeu: 2.972 ± 0.488
1.454SerMet: 1.454 ± 0.299
1.77SerAsn: 1.77 ± 0.497
2.213SerPro: 2.213 ± 0.308
2.15SerGln: 2.15 ± 0.451
3.92SerArg: 3.92 ± 0.607
2.592SerSer: 2.592 ± 0.454
2.782SerThr: 2.782 ± 0.624
3.414SerVal: 3.414 ± 0.428
1.328SerTrp: 1.328 ± 0.25
1.77SerTyr: 1.77 ± 0.332
0.0SerXaa: 0.0 ± 0.0
Thr
5.628ThrAla: 5.628 ± 0.965
0.379ThrCys: 0.379 ± 0.15
3.984ThrAsp: 3.984 ± 0.429
4.806ThrGlu: 4.806 ± 0.492
1.96ThrPhe: 1.96 ± 0.389
5.628ThrGly: 5.628 ± 0.879
1.265ThrHis: 1.265 ± 0.319
3.035ThrIle: 3.035 ± 0.395
2.403ThrLys: 2.403 ± 0.396
4.869ThrLeu: 4.869 ± 0.689
1.707ThrMet: 1.707 ± 0.37
2.023ThrAsn: 2.023 ± 0.351
4.3ThrPro: 4.3 ± 0.618
1.897ThrGln: 1.897 ± 0.38
2.845ThrArg: 2.845 ± 0.376
2.592ThrSer: 2.592 ± 0.413
3.92ThrThr: 3.92 ± 0.766
4.236ThrVal: 4.236 ± 0.473
1.201ThrTrp: 1.201 ± 0.254
1.834ThrTyr: 1.834 ± 0.323
0.0ThrXaa: 0.0 ± 0.0
Val
6.197ValAla: 6.197 ± 0.642
1.075ValCys: 1.075 ± 0.258
6.07ValAsp: 6.07 ± 0.713
6.133ValGlu: 6.133 ± 0.705
1.897ValPhe: 1.897 ± 0.321
6.007ValGly: 6.007 ± 0.588
1.075ValHis: 1.075 ± 0.254
4.553ValIle: 4.553 ± 0.657
3.098ValLys: 3.098 ± 0.432
5.122ValLeu: 5.122 ± 0.728
1.391ValMet: 1.391 ± 0.276
2.719ValAsn: 2.719 ± 0.544
2.972ValPro: 2.972 ± 0.449
2.15ValGln: 2.15 ± 0.323
4.047ValArg: 4.047 ± 0.519
4.616ValSer: 4.616 ± 0.506
4.932ValThr: 4.932 ± 0.524
5.817ValVal: 5.817 ± 0.692
1.581ValTrp: 1.581 ± 0.274
2.466ValTyr: 2.466 ± 0.506
0.0ValXaa: 0.0 ± 0.0
Trp
2.023TrpAla: 2.023 ± 0.428
0.253TrpCys: 0.253 ± 0.128
1.834TrpAsp: 1.834 ± 0.32
1.328TrpGlu: 1.328 ± 0.304
0.506TrpPhe: 0.506 ± 0.179
1.644TrpGly: 1.644 ± 0.346
0.506TrpHis: 0.506 ± 0.202
1.328TrpIle: 1.328 ± 0.368
0.632TrpLys: 0.632 ± 0.198
1.265TrpLeu: 1.265 ± 0.287
0.632TrpMet: 0.632 ± 0.183
0.759TrpAsn: 0.759 ± 0.252
0.696TrpPro: 0.696 ± 0.219
0.885TrpGln: 0.885 ± 0.233
1.075TrpArg: 1.075 ± 0.285
1.518TrpSer: 1.518 ± 0.264
1.012TrpThr: 1.012 ± 0.261
1.454TrpVal: 1.454 ± 0.301
0.759TrpTrp: 0.759 ± 0.219
0.443TrpTyr: 0.443 ± 0.152
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.845TyrAla: 2.845 ± 0.477
0.063TyrCys: 0.063 ± 0.067
2.529TyrAsp: 2.529 ± 0.489
2.403TyrGlu: 2.403 ± 0.447
0.759TyrPhe: 0.759 ± 0.264
2.782TyrGly: 2.782 ± 0.501
0.759TyrHis: 0.759 ± 0.239
1.96TyrIle: 1.96 ± 0.332
0.632TyrLys: 0.632 ± 0.223
2.719TyrLeu: 2.719 ± 0.397
0.632TyrMet: 0.632 ± 0.192
1.075TyrAsn: 1.075 ± 0.289
1.454TyrPro: 1.454 ± 0.272
1.075TyrGln: 1.075 ± 0.244
2.403TyrArg: 2.403 ± 0.466
1.138TyrSer: 1.138 ± 0.312
1.707TyrThr: 1.707 ± 0.349
2.592TyrVal: 2.592 ± 0.415
0.506TyrTrp: 0.506 ± 0.19
0.822TyrTyr: 0.822 ± 0.21
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 82 proteins (15816 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski