Spiroplasma virus SpV1-C74 (SpV1)

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Plectroviridae; Vespertiliovirus; Spiroplasma virus C74

Average proteome isoelectric point is 8.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 13 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q88427|ORF2_SPV1C Uncharacterized protein ORF2 OS=Spiroplasma virus SpV1-C74 OX=185959 GN=ORF2 PE=3 SV=1
MM1 pKa = 7.09QNDD4 pKa = 3.16WQEE7 pKa = 3.94FKK9 pKa = 11.24EE10 pKa = 3.91FFIYY14 pKa = 10.26IFLFIDD20 pKa = 3.43KK21 pKa = 10.52ANVEE25 pKa = 4.75SIIMWNLTQNEE36 pKa = 4.89YY37 pKa = 9.22LTLMVGVWVVILFLTWFFLWMVFKK61 pKa = 10.77IVGYY65 pKa = 9.59FKK67 pKa = 11.09

Molecular weight: 8.39 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q88417|ORF15_SPV1C Uncharacterized protein ORF15 OS=Spiroplasma virus SpV1-C74 OX=185959 GN=ORF15 PE=4 SV=1
MM1 pKa = 7.51VKK3 pKa = 9.98MINLLVVEE11 pKa = 4.84NNNSNWDD18 pKa = 3.76KK19 pKa = 10.97IFSFVFDD26 pKa = 3.42IFLFIFDD33 pKa = 4.91VIWNTKK39 pKa = 10.34LPMTNTSIAYY49 pKa = 8.33FLVFFMVIKK58 pKa = 10.42LSIYY62 pKa = 9.85AIHH65 pKa = 6.33GTSTQYY71 pKa = 11.67NNLGSTVNNGVSQVYY86 pKa = 10.36SSTVRR91 pKa = 11.84KK92 pKa = 9.62GINVGKK98 pKa = 10.05NVYY101 pKa = 10.03QNSNKK106 pKa = 9.42QQVKK110 pKa = 10.36KK111 pKa = 9.78EE112 pKa = 4.23LKK114 pKa = 8.82RR115 pKa = 11.84QSIRR119 pKa = 11.84YY120 pKa = 5.3QAKK123 pKa = 9.81NIRR126 pKa = 11.84STKK129 pKa = 10.22FKK131 pKa = 10.76GDD133 pKa = 3.12KK134 pKa = 10.15KK135 pKa = 10.98

Molecular weight: 15.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

13

0

13

2197

36

713

169.0

20.13

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.868 ± 0.301

0.546 ± 0.257

4.461 ± 0.651

4.324 ± 0.481

8.739 ± 0.509

4.188 ± 0.379

0.91 ± 0.211

9.422 ± 0.875

10.332 ± 1.022

9.968 ± 0.72

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.185 ± 0.499

8.557 ± 1.233

2.094 ± 0.257

3.459 ± 0.515

2.822 ± 0.345

5.872 ± 0.449

4.506 ± 0.419

5.963 ± 0.568

2.594 ± 0.443

6.19 ± 0.662
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski