Amino acid dipepetide frequency for Microbulbifer sp. A4B17

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.796AlaAla: 8.796 ± 0.124
1.021AlaCys: 1.021 ± 0.028
5.057AlaAsp: 5.057 ± 0.11
6.431AlaGlu: 6.431 ± 0.097
3.254AlaPhe: 3.254 ± 0.057
6.971AlaGly: 6.971 ± 0.084
1.656AlaHis: 1.656 ± 0.037
5.412AlaIle: 5.412 ± 0.062
3.704AlaLys: 3.704 ± 0.059
9.814AlaLeu: 9.814 ± 0.12
2.251AlaMet: 2.251 ± 0.047
3.071AlaAsn: 3.071 ± 0.047
3.439AlaPro: 3.439 ± 0.059
3.746AlaGln: 3.746 ± 0.064
4.657AlaArg: 4.657 ± 0.064
5.454AlaSer: 5.454 ± 0.074
4.28AlaThr: 4.28 ± 0.077
6.195AlaVal: 6.195 ± 0.082
1.045AlaTrp: 1.045 ± 0.034
2.475AlaTyr: 2.475 ± 0.05
0.0AlaXaa: 0.0 ± 0.0
Cys
0.975CysAla: 0.975 ± 0.03
0.154CysCys: 0.154 ± 0.012
0.593CysAsp: 0.593 ± 0.028
0.652CysGlu: 0.652 ± 0.021
0.414CysPhe: 0.414 ± 0.016
0.991CysGly: 0.991 ± 0.031
0.338CysHis: 0.338 ± 0.019
0.553CysIle: 0.553 ± 0.02
0.391CysLys: 0.391 ± 0.016
1.019CysLeu: 1.019 ± 0.03
0.202CysMet: 0.202 ± 0.013
0.395CysAsn: 0.395 ± 0.015
0.514CysPro: 0.514 ± 0.025
0.364CysGln: 0.364 ± 0.016
0.593CysArg: 0.593 ± 0.023
0.808CysSer: 0.808 ± 0.023
0.516CysThr: 0.516 ± 0.02
0.606CysVal: 0.606 ± 0.021
0.151CysTrp: 0.151 ± 0.012
0.35CysTyr: 0.35 ± 0.017
0.0CysXaa: 0.0 ± 0.0
Asp
4.351AspAla: 4.351 ± 0.084
0.59AspCys: 0.59 ± 0.023
2.949AspAsp: 2.949 ± 0.079
3.56AspGlu: 3.56 ± 0.061
2.488AspPhe: 2.488 ± 0.05
4.535AspGly: 4.535 ± 0.104
1.108AspHis: 1.108 ± 0.03
3.817AspIle: 3.817 ± 0.064
2.67AspLys: 2.67 ± 0.049
5.71AspLeu: 5.71 ± 0.074
1.311AspMet: 1.311 ± 0.034
2.483AspAsn: 2.483 ± 0.07
2.481AspPro: 2.481 ± 0.05
2.147AspGln: 2.147 ± 0.044
2.915AspArg: 2.915 ± 0.048
4.085AspSer: 4.085 ± 0.105
2.946AspThr: 2.946 ± 0.071
3.292AspVal: 3.292 ± 0.064
0.998AspTrp: 0.998 ± 0.032
2.174AspTyr: 2.174 ± 0.047
0.0AspXaa: 0.0 ± 0.0
Glu
5.704GluAla: 5.704 ± 0.086
0.51GluCys: 0.51 ± 0.021
3.391GluAsp: 3.391 ± 0.055
4.608GluGlu: 4.608 ± 0.08
2.469GluPhe: 2.469 ± 0.049
4.451GluGly: 4.451 ± 0.061
1.372GluHis: 1.372 ± 0.032
3.968GluIle: 3.968 ± 0.059
3.606GluLys: 3.606 ± 0.067
7.041GluLeu: 7.041 ± 0.088
1.563GluMet: 1.563 ± 0.036
2.665GluAsn: 2.665 ± 0.048
2.38GluPro: 2.38 ± 0.042
3.486GluGln: 3.486 ± 0.065
4.074GluArg: 4.074 ± 0.065
4.167GluSer: 4.167 ± 0.059
3.05GluThr: 3.05 ± 0.059
4.652GluVal: 4.652 ± 0.073
0.866GluTrp: 0.866 ± 0.024
1.986GluTyr: 1.986 ± 0.038
0.0GluXaa: 0.0 ± 0.0
Phe
3.491PheAla: 3.491 ± 0.051
0.51PheCys: 0.51 ± 0.02
2.603PheAsp: 2.603 ± 0.044
2.364PheGlu: 2.364 ± 0.045
1.803PhePhe: 1.803 ± 0.047
3.182PheGly: 3.182 ± 0.056
0.818PheHis: 0.818 ± 0.028
2.36PheIle: 2.36 ± 0.047
1.556PheLys: 1.556 ± 0.037
3.71PheLeu: 3.71 ± 0.061
0.839PheMet: 0.839 ± 0.027
1.735PheAsn: 1.735 ± 0.036
1.621PhePro: 1.621 ± 0.036
1.26PheGln: 1.26 ± 0.031
1.935PheArg: 1.935 ± 0.043
3.57PheSer: 3.57 ± 0.07
2.264PheThr: 2.264 ± 0.049
2.48PheVal: 2.48 ± 0.045
0.574PheTrp: 0.574 ± 0.02
1.401PheTyr: 1.401 ± 0.038
0.0PheXaa: 0.0 ± 0.0
Gly
6.258GlyAla: 6.258 ± 0.083
0.943GlyCys: 0.943 ± 0.025
4.553GlyAsp: 4.553 ± 0.102
5.128GlyGlu: 5.128 ± 0.08
3.293GlyPhe: 3.293 ± 0.046
6.006GlyGly: 6.006 ± 0.112
1.64GlyHis: 1.64 ± 0.035
4.738GlyIle: 4.738 ± 0.069
3.542GlyLys: 3.542 ± 0.057
7.227GlyLeu: 7.227 ± 0.098
1.867GlyMet: 1.867 ± 0.04
3.24GlyAsn: 3.24 ± 0.074
2.261GlyPro: 2.261 ± 0.047
2.773GlyGln: 2.773 ± 0.047
4.188GlyArg: 4.188 ± 0.066
5.129GlySer: 5.129 ± 0.097
3.903GlyThr: 3.903 ± 0.107
5.44GlyVal: 5.44 ± 0.078
1.111GlyTrp: 1.111 ± 0.03
2.711GlyTyr: 2.711 ± 0.045
0.0GlyXaa: 0.0 ± 0.0
His
1.426HisAla: 1.426 ± 0.032
0.334HisCys: 0.334 ± 0.016
0.874HisAsp: 0.874 ± 0.026
0.997HisGlu: 0.997 ± 0.026
1.096HisPhe: 1.096 ± 0.029
1.562HisGly: 1.562 ± 0.034
0.568HisHis: 0.568 ± 0.023
1.181HisIle: 1.181 ± 0.03
0.91HisLys: 0.91 ± 0.027
2.289HisLeu: 2.289 ± 0.044
0.464HisMet: 0.464 ± 0.021
0.818HisAsn: 0.818 ± 0.028
1.146HisPro: 1.146 ± 0.03
0.941HisGln: 0.941 ± 0.028
1.318HisArg: 1.318 ± 0.033
1.493HisSer: 1.493 ± 0.033
1.016HisThr: 1.016 ± 0.028
1.037HisVal: 1.037 ± 0.027
0.446HisTrp: 0.446 ± 0.018
0.783HisTyr: 0.783 ± 0.025
0.0HisXaa: 0.0 ± 0.0
Ile
5.886IleAla: 5.886 ± 0.072
0.716IleCys: 0.716 ± 0.021
3.83IleAsp: 3.83 ± 0.063
4.259IleGlu: 4.259 ± 0.065
2.266IlePhe: 2.266 ± 0.048
4.408IleGly: 4.408 ± 0.06
1.18IleHis: 1.18 ± 0.031
2.866IleIle: 2.866 ± 0.054
2.48IleLys: 2.48 ± 0.052
5.052IleLeu: 5.052 ± 0.077
1.004IleMet: 1.004 ± 0.028
2.593IleAsn: 2.593 ± 0.05
2.676IlePro: 2.676 ± 0.052
2.02IleGln: 2.02 ± 0.04
2.854IleArg: 2.854 ± 0.045
4.456IleSer: 4.456 ± 0.062
3.266IleThr: 3.266 ± 0.067
3.682IleVal: 3.682 ± 0.071
0.728IleTrp: 0.728 ± 0.024
1.935IleTyr: 1.935 ± 0.036
0.0IleXaa: 0.0 ± 0.0
Lys
3.913LysAla: 3.913 ± 0.069
0.292LysCys: 0.292 ± 0.015
2.277LysAsp: 2.277 ± 0.046
2.76LysGlu: 2.76 ± 0.056
1.423LysPhe: 1.423 ± 0.032
2.984LysGly: 2.984 ± 0.05
0.891LysHis: 0.891 ± 0.027
2.657LysIle: 2.657 ± 0.05
2.496LysLys: 2.496 ± 0.064
4.729LysLeu: 4.729 ± 0.071
1.056LysMet: 1.056 ± 0.031
1.833LysAsn: 1.833 ± 0.043
2.113LysPro: 2.113 ± 0.041
1.893LysGln: 1.893 ± 0.042
2.572LysArg: 2.572 ± 0.049
2.882LysSer: 2.882 ± 0.052
2.283LysThr: 2.283 ± 0.047
3.319LysVal: 3.319 ± 0.048
0.476LysTrp: 0.476 ± 0.02
1.298LysTyr: 1.298 ± 0.036
0.0LysXaa: 0.0 ± 0.0
Leu
10.119LeuAla: 10.119 ± 0.114
1.126LeuCys: 1.126 ± 0.028
5.767LeuAsp: 5.767 ± 0.081
6.65LeuGlu: 6.65 ± 0.087
3.986LeuPhe: 3.986 ± 0.063
7.466LeuGly: 7.466 ± 0.094
2.057LeuHis: 2.057 ± 0.045
5.791LeuIle: 5.791 ± 0.08
4.57LeuLys: 4.57 ± 0.066
11.11LeuLeu: 11.11 ± 0.169
2.336LeuMet: 2.336 ± 0.045
4.029LeuAsn: 4.029 ± 0.052
5.099LeuPro: 5.099 ± 0.074
4.39LeuGln: 4.39 ± 0.072
5.765LeuArg: 5.765 ± 0.086
7.674LeuSer: 7.674 ± 0.08
5.341LeuThr: 5.341 ± 0.113
6.973LeuVal: 6.973 ± 0.082
1.221LeuTrp: 1.221 ± 0.031
2.686LeuTyr: 2.686 ± 0.047
0.0LeuXaa: 0.0 ± 0.0
Met
2.404MetAla: 2.404 ± 0.047
0.184MetCys: 0.184 ± 0.013
1.189MetAsp: 1.189 ± 0.031
1.387MetGlu: 1.387 ± 0.036
0.674MetPhe: 0.674 ± 0.022
1.68MetGly: 1.68 ± 0.039
0.451MetHis: 0.451 ± 0.018
1.171MetIle: 1.171 ± 0.031
1.108MetLys: 1.108 ± 0.029
2.28MetLeu: 2.28 ± 0.041
0.517MetMet: 0.517 ± 0.019
0.858MetAsn: 0.858 ± 0.028
1.197MetPro: 1.197 ± 0.034
0.999MetGln: 0.999 ± 0.028
1.349MetArg: 1.349 ± 0.032
1.63MetSer: 1.63 ± 0.033
1.188MetThr: 1.188 ± 0.033
1.59MetVal: 1.59 ± 0.037
0.214MetTrp: 0.214 ± 0.013
0.459MetTyr: 0.459 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
2.981AsnAla: 2.981 ± 0.054
0.465AsnCys: 0.465 ± 0.023
1.995AsnAsp: 1.995 ± 0.06
1.994AsnGlu: 1.994 ± 0.048
1.726AsnPhe: 1.726 ± 0.038
3.106AsnGly: 3.106 ± 0.063
0.85AsnHis: 0.85 ± 0.028
2.427AsnIle: 2.427 ± 0.049
1.64AsnLys: 1.64 ± 0.04
4.063AsnLeu: 4.063 ± 0.058
0.854AsnMet: 0.854 ± 0.026
1.866AsnAsn: 1.866 ± 0.05
2.177AsnPro: 2.177 ± 0.043
1.602AsnGln: 1.602 ± 0.042
2.236AsnArg: 2.236 ± 0.041
2.914AsnSer: 2.914 ± 0.062
2.266AsnThr: 2.266 ± 0.053
2.083AsnVal: 2.083 ± 0.037
0.661AsnTrp: 0.661 ± 0.023
1.49AsnTyr: 1.49 ± 0.04
0.0AsnXaa: 0.0 ± 0.0
Pro
3.673ProAla: 3.673 ± 0.059
0.324ProCys: 0.324 ± 0.014
2.686ProAsp: 2.686 ± 0.043
3.635ProGlu: 3.635 ± 0.065
1.681ProPhe: 1.681 ± 0.038
3.256ProGly: 3.256 ± 0.062
0.875ProHis: 0.875 ± 0.025
2.315ProIle: 2.315 ± 0.048
1.943ProLys: 1.943 ± 0.045
4.531ProLeu: 4.531 ± 0.068
0.95ProMet: 0.95 ± 0.026
1.573ProAsn: 1.573 ± 0.038
1.606ProPro: 1.606 ± 0.044
1.999ProGln: 1.999 ± 0.043
1.855ProArg: 1.855 ± 0.044
2.709ProSer: 2.709 ± 0.05
2.094ProThr: 2.094 ± 0.049
3.377ProVal: 3.377 ± 0.054
0.574ProTrp: 0.574 ± 0.021
1.263ProTyr: 1.263 ± 0.031
0.0ProXaa: 0.0 ± 0.0
Gln
3.707GlnAla: 3.707 ± 0.061
0.365GlnCys: 0.365 ± 0.02
1.821GlnAsp: 1.821 ± 0.038
2.554GlnGlu: 2.554 ± 0.05
1.59GlnPhe: 1.59 ± 0.039
2.805GlnGly: 2.805 ± 0.041
0.845GlnHis: 0.845 ± 0.026
2.192GlnIle: 2.192 ± 0.043
1.834GlnLys: 1.834 ± 0.042
5.286GlnLeu: 5.286 ± 0.083
1.009GlnMet: 1.009 ± 0.028
1.351GlnAsn: 1.351 ± 0.034
1.755GlnPro: 1.755 ± 0.036
2.434GlnGln: 2.434 ± 0.053
2.644GlnArg: 2.644 ± 0.054
2.585GlnSer: 2.585 ± 0.052
1.776GlnThr: 1.776 ± 0.039
3.274GlnVal: 3.274 ± 0.056
0.717GlnTrp: 0.717 ± 0.023
1.214GlnTyr: 1.214 ± 0.033
0.0GlnXaa: 0.0 ± 0.0
Arg
4.663ArgAla: 4.663 ± 0.071
0.538ArgCys: 0.538 ± 0.019
3.182ArgAsp: 3.182 ± 0.051
4.167ArgGlu: 4.167 ± 0.068
2.404ArgPhe: 2.404 ± 0.044
3.602ArgGly: 3.602 ± 0.063
1.258ArgHis: 1.258 ± 0.029
3.209ArgIle: 3.209 ± 0.048
2.604ArgLys: 2.604 ± 0.051
5.688ArgLeu: 5.688 ± 0.078
1.26ArgMet: 1.26 ± 0.036
2.122ArgAsn: 2.122 ± 0.038
2.137ArgPro: 2.137 ± 0.037
2.418ArgGln: 2.418 ± 0.046
3.396ArgArg: 3.396 ± 0.065
3.341ArgSer: 3.341 ± 0.052
2.412ArgThr: 2.412 ± 0.046
3.647ArgVal: 3.647 ± 0.061
0.899ArgTrp: 0.899 ± 0.029
1.965ArgTyr: 1.965 ± 0.04
0.0ArgXaa: 0.0 ± 0.0
Ser
6.217SerAla: 6.217 ± 0.101
0.725SerCys: 0.725 ± 0.028
4.086SerAsp: 4.086 ± 0.091
4.456SerGlu: 4.456 ± 0.063
2.851SerPhe: 2.851 ± 0.056
6.353SerGly: 6.353 ± 0.094
1.448SerHis: 1.448 ± 0.033
3.958SerIle: 3.958 ± 0.052
2.746SerLys: 2.746 ± 0.056
7.175SerLeu: 7.175 ± 0.083
1.531SerMet: 1.531 ± 0.03
2.655SerAsn: 2.655 ± 0.058
2.859SerPro: 2.859 ± 0.054
2.63SerGln: 2.63 ± 0.049
3.554SerArg: 3.554 ± 0.053
5.2SerSer: 5.2 ± 0.105
3.432SerThr: 3.432 ± 0.058
4.489SerVal: 4.489 ± 0.077
1.004SerTrp: 1.004 ± 0.031
2.29SerTyr: 2.29 ± 0.051
0.0SerXaa: 0.0 ± 0.0
Thr
4.644ThrAla: 4.644 ± 0.082
0.485ThrCys: 0.485 ± 0.02
3.032ThrAsp: 3.032 ± 0.077
3.191ThrGlu: 3.191 ± 0.052
1.98ThrPhe: 1.98 ± 0.055
4.437ThrGly: 4.437 ± 0.123
0.98ThrHis: 0.98 ± 0.026
2.928ThrIle: 2.928 ± 0.057
1.727ThrLys: 1.727 ± 0.039
5.834ThrLeu: 5.834 ± 0.077
0.967ThrMet: 0.967 ± 0.027
1.805ThrAsn: 1.805 ± 0.045
2.591ThrPro: 2.591 ± 0.047
1.948ThrGln: 1.948 ± 0.04
2.308ThrArg: 2.308 ± 0.043
3.391ThrSer: 3.391 ± 0.086
2.72ThrThr: 2.72 ± 0.058
3.696ThrVal: 3.696 ± 0.068
0.654ThrTrp: 0.654 ± 0.026
1.575ThrTyr: 1.575 ± 0.044
0.0ThrXaa: 0.0 ± 0.0
Val
6.336ValAla: 6.336 ± 0.078
0.737ValCys: 0.737 ± 0.024
4.202ValAsp: 4.202 ± 0.061
4.804ValGlu: 4.804 ± 0.063
2.636ValPhe: 2.636 ± 0.052
4.722ValGly: 4.722 ± 0.068
1.27ValHis: 1.27 ± 0.031
4.188ValIle: 4.188 ± 0.064
2.825ValLys: 2.825 ± 0.05
6.4ValLeu: 6.4 ± 0.088
1.609ValMet: 1.609 ± 0.034
2.616ValAsn: 2.616 ± 0.049
2.917ValPro: 2.917 ± 0.054
2.287ValGln: 2.287 ± 0.044
3.462ValArg: 3.462 ± 0.057
4.91ValSer: 4.91 ± 0.069
3.894ValThr: 3.894 ± 0.088
4.986ValVal: 4.986 ± 0.068
0.793ValTrp: 0.793 ± 0.026
1.823ValTyr: 1.823 ± 0.04
0.0ValXaa: 0.0 ± 0.0
Trp
0.93TrpAla: 0.93 ± 0.026
0.161TrpCys: 0.161 ± 0.011
0.739TrpAsp: 0.739 ± 0.029
0.721TrpGlu: 0.721 ± 0.027
0.571TrpPhe: 0.571 ± 0.02
0.97TrpGly: 0.97 ± 0.029
0.385TrpHis: 0.385 ± 0.018
0.771TrpIle: 0.771 ± 0.025
0.579TrpLys: 0.579 ± 0.021
1.827TrpLeu: 1.827 ± 0.039
0.347TrpMet: 0.347 ± 0.015
0.513TrpAsn: 0.513 ± 0.023
0.543TrpPro: 0.543 ± 0.02
0.842TrpGln: 0.842 ± 0.027
0.948TrpArg: 0.948 ± 0.027
0.926TrpSer: 0.926 ± 0.031
0.565TrpThr: 0.565 ± 0.026
0.918TrpVal: 0.918 ± 0.026
0.239TrpTrp: 0.239 ± 0.014
0.447TrpTyr: 0.447 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.257TyrAla: 2.257 ± 0.043
0.396TyrCys: 0.396 ± 0.018
1.783TyrAsp: 1.783 ± 0.058
1.695TyrGlu: 1.695 ± 0.04
1.471TyrPhe: 1.471 ± 0.032
2.462TyrGly: 2.462 ± 0.063
0.713TyrHis: 0.713 ± 0.025
1.656TyrIle: 1.656 ± 0.037
1.217TyrLys: 1.217 ± 0.029
3.371TyrLeu: 3.371 ± 0.058
0.59TyrMet: 0.59 ± 0.023
1.176TyrAsn: 1.176 ± 0.032
1.406TyrPro: 1.406 ± 0.031
1.576TyrGln: 1.576 ± 0.033
2.286TyrArg: 2.286 ± 0.046
2.337TyrSer: 2.337 ± 0.047
1.681TyrThr: 1.681 ± 0.039
1.68TyrVal: 1.68 ± 0.034
0.572TyrTrp: 0.572 ± 0.022
1.132TyrTyr: 1.132 ± 0.036
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4240 proteins (1368767 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski