Anopheles sinensis (Mosquito)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Protostomia; Ecdysozoa; Panarthropoda; Arthropoda; Mandibulata; Pancrustacea; Hexapoda; Insecta; Dicondylia; Pterygota; Neoptera; Endopterygota; Diptera; Nematocera; Culicomorpha; Culicoidea; Culicidae;

Average proteome isoelectric point is 7.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 19343 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A084W8K2|A0A084W8K2_ANOSI Biotin-protein ligase OS=Anopheles sinensis OX=74873 GN=ZHAS_00014545 PE=4 SV=1
MM1 pKa = 7.38RR2 pKa = 11.84TFGFAVATVLCAIQLTAALPRR23 pKa = 11.84PDD25 pKa = 4.38FGLSIDD31 pKa = 4.27VYY33 pKa = 11.11SADD36 pKa = 3.69KK37 pKa = 10.81TSDD40 pKa = 3.32VADD43 pKa = 3.88AANNDD48 pKa = 4.24LVTLWSTSTITLNANYY64 pKa = 7.7DD65 pKa = 3.82TLGEE69 pKa = 4.06ILTEE73 pKa = 3.79ITAIGTDD80 pKa = 3.13ASANIKK86 pKa = 10.43AVFDD90 pKa = 4.88AIIDD94 pKa = 4.04LADD97 pKa = 3.6ATTDD101 pKa = 3.17PTAAFDD107 pKa = 3.89AAIDD111 pKa = 3.93AVDD114 pKa = 4.0ALITYY119 pKa = 7.73ITSEE123 pKa = 4.38TVGLSVNYY131 pKa = 10.46GNLDD135 pKa = 3.51TALSTNEE142 pKa = 3.79IVVQLEE148 pKa = 4.07DD149 pKa = 4.16AFGEE153 pKa = 4.29LADD156 pKa = 3.9GLARR160 pKa = 11.84LGAALTALKK169 pKa = 10.36AQVLAAVTTAGSGTVTKK186 pKa = 10.73AILRR190 pKa = 11.84QKK192 pKa = 10.93LSIADD197 pKa = 3.82TSDD200 pKa = 3.14LTVSVVDD207 pKa = 4.06VSSSLQLIFFILEE220 pKa = 3.66QSKK223 pKa = 10.44EE224 pKa = 3.95FLQLADD230 pKa = 4.27AYY232 pKa = 9.68IISSGVAGQDD242 pKa = 2.85AVDD245 pKa = 4.29YY246 pKa = 10.9INEE249 pKa = 3.96QLQVLSGEE257 pKa = 4.23VEE259 pKa = 4.4SYY261 pKa = 11.2KK262 pKa = 10.92DD263 pKa = 3.19AADD266 pKa = 4.16TIKK269 pKa = 8.15TTVTDD274 pKa = 3.83TFDD277 pKa = 3.32GSTDD281 pKa = 3.11LGARR285 pKa = 11.84DD286 pKa = 4.22LTPIAAIKK294 pKa = 9.72TEE296 pKa = 3.68VDD298 pKa = 3.06ATVTTFGANIDD309 pKa = 3.94TAVTAIEE316 pKa = 4.18ASFTGYY322 pKa = 9.97QADD325 pKa = 3.5ILLVSTGYY333 pKa = 9.17ATFYY337 pKa = 10.96SDD339 pKa = 3.71NACGHH344 pKa = 6.35IFDD347 pKa = 5.46LVTVLINQGPYY358 pKa = 10.54AEE360 pKa = 4.34YY361 pKa = 10.37CYY363 pKa = 10.79EE364 pKa = 4.15KK365 pKa = 11.26YY366 pKa = 11.02GDD368 pKa = 3.65AALALFDD375 pKa = 4.39SNARR379 pKa = 11.84VAGEE383 pKa = 4.12CVDD386 pKa = 4.57RR387 pKa = 11.84EE388 pKa = 4.18ITRR391 pKa = 11.84LLTLQDD397 pKa = 3.62VLISIAEE404 pKa = 3.94QIAFSVEE411 pKa = 3.9DD412 pKa = 4.27LLATLDD418 pKa = 3.68FCLNSVTEE426 pKa = 4.77CNDD429 pKa = 2.88SDD431 pKa = 3.49IDD433 pKa = 3.82MYY435 pKa = 11.29LGAIHH440 pKa = 7.13AEE442 pKa = 3.69IDD444 pKa = 3.48ASHH447 pKa = 6.97LEE449 pKa = 4.09VLTDD453 pKa = 4.3LVDD456 pKa = 4.95DD457 pKa = 4.19EE458 pKa = 4.9TDD460 pKa = 2.93AGLARR465 pKa = 11.84LQACFVAARR474 pKa = 11.84YY475 pKa = 9.46DD476 pKa = 3.35ILRR479 pKa = 11.84EE480 pKa = 3.82IDD482 pKa = 4.62GMTTDD487 pKa = 4.3IAACEE492 pKa = 4.3TNGPNPPSPRR502 pKa = 3.46

Molecular weight: 53.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A084VFU9|A0A084VFU9_ANOSI Probable U3 small nucleolar RNA-associated protein 11 OS=Anopheles sinensis OX=74873 GN=ZHAS_00004010 PE=3 SV=1
MM1 pKa = 7.16MNAAILIGLGMISSPNSHH19 pKa = 7.23LKK21 pKa = 10.32RR22 pKa = 11.84SRR24 pKa = 11.84NSLPSRR30 pKa = 11.84GLPFLFLPRR39 pKa = 11.84RR40 pKa = 11.84LNKK43 pKa = 9.4QANNGKK49 pKa = 8.31GTVFQGRR56 pKa = 11.84RR57 pKa = 11.84SAPGGKK63 pKa = 9.54GSKK66 pKa = 9.68KK67 pKa = 9.88RR68 pKa = 11.84ALL70 pKa = 3.66

Molecular weight: 7.56 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

19343

0

19343

7280472

47

11741

376.4

41.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.327 ± 0.018

1.919 ± 0.017

5.214 ± 0.015

6.241 ± 0.025

3.571 ± 0.015

6.893 ± 0.03

2.682 ± 0.014

4.744 ± 0.018

5.248 ± 0.022

8.738 ± 0.03

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.289 ± 0.009

4.403 ± 0.013

5.656 ± 0.025

4.373 ± 0.018

6.076 ± 0.02

8.23 ± 0.033

6.079 ± 0.02

6.341 ± 0.015

1.038 ± 0.007

2.886 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski