Micromonospora narathiwatensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micromonosporales; Micromonosporaceae; Micromonospora

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5762 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1A9ACK7|A0A1A9ACK7_9ACTN Threonine dehydratase OS=Micromonospora narathiwatensis OX=299146 GN=GA0070621_5019 PE=4 SV=1
MM1 pKa = 6.64VTTMYY6 pKa = 10.11QVQGMTCGHH15 pKa = 6.59CVNSVNAEE23 pKa = 3.72VGAIPGVSDD32 pKa = 3.61VQVDD36 pKa = 4.18LASGRR41 pKa = 11.84VTVTSEE47 pKa = 4.19SPLDD51 pKa = 3.66TDD53 pKa = 3.8TVRR56 pKa = 11.84AAVDD60 pKa = 3.1EE61 pKa = 4.62AGYY64 pKa = 11.0DD65 pKa = 3.52LVGAA69 pKa = 4.97

Molecular weight: 7.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1A8ZYG8|A0A1A8ZYG8_9ACTN Predicted arabinose efflux permease MFS family OS=Micromonospora narathiwatensis OX=299146 GN=GA0070621_3295 PE=4 SV=1
MM1 pKa = 7.91AEE3 pKa = 4.14AQQATTRR10 pKa = 11.84PAAAPRR16 pKa = 11.84TRR18 pKa = 11.84KK19 pKa = 7.83QTSAARR25 pKa = 11.84RR26 pKa = 11.84ITGAAGTTTVTKK38 pKa = 10.59ASPNASGAGAGRR50 pKa = 11.84VPATKK55 pKa = 10.3AVAKK59 pKa = 10.08KK60 pKa = 9.72AAAKK64 pKa = 10.03KK65 pKa = 9.75VVSAARR71 pKa = 11.84KK72 pKa = 9.25APAKK76 pKa = 9.92AAPATKK82 pKa = 8.32TTAKK86 pKa = 10.12RR87 pKa = 11.84APATRR92 pKa = 11.84ATAKK96 pKa = 10.16KK97 pKa = 10.5APATAKK103 pKa = 10.21KK104 pKa = 10.56APATKK109 pKa = 9.25ATAKK113 pKa = 9.11KK114 pKa = 6.69TTAKK118 pKa = 9.81QAAAGRR124 pKa = 11.84TTAAAKK130 pKa = 9.41KK131 pKa = 10.22APAKK135 pKa = 7.31TTAAKK140 pKa = 9.92KK141 pKa = 10.18APARR145 pKa = 11.84KK146 pKa = 5.82TTAAATGAPAKK157 pKa = 9.45RR158 pKa = 11.84TTTAAAKK165 pKa = 9.25KK166 pKa = 9.97APARR170 pKa = 11.84ATAAARR176 pKa = 11.84KK177 pKa = 7.71ATSTTTKK184 pKa = 9.43ATAAAKK190 pKa = 10.28KK191 pKa = 10.37AGTAAKK197 pKa = 9.78KK198 pKa = 10.04AAAKK202 pKa = 6.76TTTKK206 pKa = 10.37RR207 pKa = 11.84APAKK211 pKa = 9.21RR212 pKa = 11.84TTAAAKK218 pKa = 9.35KK219 pKa = 10.38APAAKK224 pKa = 9.86KK225 pKa = 10.41APAKK229 pKa = 9.31KK230 pKa = 6.73TTAAAKK236 pKa = 7.44TAPAKK241 pKa = 9.47KK242 pKa = 6.79TTAAKK247 pKa = 9.96KK248 pKa = 10.45APAKK252 pKa = 10.32KK253 pKa = 9.09ATAVKK258 pKa = 10.3AAGTRR263 pKa = 11.84PAGGARR269 pKa = 11.84KK270 pKa = 8.75AAAKK274 pKa = 9.28KK275 pKa = 10.11APARR279 pKa = 11.84KK280 pKa = 6.22TTATSSTAARR290 pKa = 11.84KK291 pKa = 8.84AAGGRR296 pKa = 11.84KK297 pKa = 7.88ATSAASGVSAIEE309 pKa = 4.09ARR311 pKa = 11.84TSTSTGTHH319 pKa = 5.61KK320 pKa = 10.95RR321 pKa = 11.84LTAARR326 pKa = 11.84KK327 pKa = 7.84PAGTRR332 pKa = 11.84VVAGRR337 pKa = 11.84STAKK341 pKa = 10.08PGSARR346 pKa = 11.84RR347 pKa = 11.84ATGG350 pKa = 2.87

Molecular weight: 33.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5762

0

5762

1962101

31

5911

340.5

36.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.137 ± 0.047

0.748 ± 0.008

5.976 ± 0.027

5.037 ± 0.032

2.616 ± 0.018

9.428 ± 0.03

2.113 ± 0.022

3.076 ± 0.022

1.669 ± 0.025

10.582 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.557 ± 0.012

1.837 ± 0.023

6.502 ± 0.033

2.678 ± 0.018

8.58 ± 0.041

4.58 ± 0.025

6.13 ± 0.037

9.021 ± 0.034

1.613 ± 0.012

2.12 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski