Vibrio phage CKB-S2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae

Average proteome isoelectric point is 6.55

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1E1GEA8|A0A1E1GEA8_9CAUD Uncharacterized protein OS=Vibrio phage CKB-S2 OX=1903260 PE=4 SV=1
MM1 pKa = 6.89TKK3 pKa = 10.85SMFHH7 pKa = 6.82LLLTLFLSVFFAFMNATKK25 pKa = 10.58ALASEE30 pKa = 4.66EE31 pKa = 3.95EE32 pKa = 4.31TTAVFVGGWSYY43 pKa = 11.5HH44 pKa = 5.81FDD46 pKa = 3.29QEE48 pKa = 3.77YY49 pKa = 10.49DD50 pKa = 3.79YY51 pKa = 11.5NEE53 pKa = 4.03SHH55 pKa = 7.08AMFAFQYY62 pKa = 9.9DD63 pKa = 3.83KK64 pKa = 11.41VVAGTFINSYY74 pKa = 9.24SDD76 pKa = 2.83RR77 pKa = 11.84GYY79 pKa = 11.62LLGYY83 pKa = 10.14DD84 pKa = 3.02SWKK87 pKa = 10.44IEE89 pKa = 4.03NEE91 pKa = 3.71YY92 pKa = 10.75GAIGTYY98 pKa = 7.68ITLMTGYY105 pKa = 10.39EE106 pKa = 4.15NFRR109 pKa = 11.84VLPMILPYY117 pKa = 10.63AQLNAFEE124 pKa = 4.69VNDD127 pKa = 3.78IEE129 pKa = 4.85IKK131 pKa = 9.36PTVLLWGKK139 pKa = 10.1AVALTINVEE148 pKa = 4.2FF149 pKa = 4.78

Molecular weight: 17.1 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1E1GE66|A0A1E1GE66_9CAUD Uncharacterized protein OS=Vibrio phage CKB-S2 OX=1903260 PE=4 SV=1
MM1 pKa = 7.6GNLPQYY7 pKa = 8.75CHH9 pKa = 6.23KK10 pKa = 10.23HH11 pKa = 4.84DD12 pKa = 4.6RR13 pKa = 11.84EE14 pKa = 4.46WIRR17 pKa = 11.84QQLGKK22 pKa = 10.44LPHH25 pKa = 6.33HH26 pKa = 6.69WRR28 pKa = 11.84EE29 pKa = 4.01QVAKK33 pKa = 10.46VYY35 pKa = 10.38SDD37 pKa = 3.35KK38 pKa = 11.35YY39 pKa = 10.15KK40 pKa = 9.85EE41 pKa = 3.96IYY43 pKa = 9.91YY44 pKa = 10.02SEE46 pKa = 4.64HH47 pKa = 6.84EE48 pKa = 4.48SPKK51 pKa = 10.07SEE53 pKa = 3.51NRR55 pKa = 11.84ARR57 pKa = 11.84NAANAWLRR65 pKa = 11.84GYY67 pKa = 7.04VTKK70 pKa = 10.91YY71 pKa = 9.96INRR74 pKa = 11.84KK75 pKa = 7.48FSKK78 pKa = 10.96VNVKK82 pKa = 10.5HH83 pKa = 5.71NN84 pKa = 3.58

Molecular weight: 10.26 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

10273

58

1059

209.7

23.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.355 ± 0.632

0.818 ± 0.191

6.084 ± 0.325

6.999 ± 0.341

3.534 ± 0.231

7.369 ± 0.383

1.84 ± 0.232

5.5 ± 0.186

6.979 ± 0.348

7.651 ± 0.329

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.93 ± 0.231

4.994 ± 0.332

3.631 ± 0.191

4.906 ± 0.392

5.14 ± 0.324

5.48 ± 0.28

5.675 ± 0.284

6.23 ± 0.343

1.441 ± 0.106

3.446 ± 0.19
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski