Vigna radiata var. radiata (Mung bean) (Phaseolus aureus)

Taxonomy: cellular organisms; Eukaryota; Viridiplantae; Streptophyta; Streptophytina; Embryophyta; Tracheophyta; Euphyllophyta; Spermatophyta; Magnoliopsida; Mesangiospermae; eudicotyledons; Gunneridae; Pentapetalae; rosids; fabids; Fabales; Fabaceae; Papilionoideae;

Average proteome isoelectric point is 6.71

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 35035 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S3UFN9|A0A1S3UFN9_VIGRR cyclic nucleotide-gated ion channel 1 OS=Vigna radiata var. radiata OX=3916 GN=LOC106764917 PE=4 SV=1
MM1 pKa = 7.66DD2 pKa = 3.28QRR4 pKa = 11.84EE5 pKa = 4.13EE6 pKa = 4.53GKK8 pKa = 9.28QQPSQKK14 pKa = 9.19TPSDD18 pKa = 3.76NQLDD22 pKa = 4.19PVDD25 pKa = 4.48SSDD28 pKa = 5.05NIAPEE33 pKa = 3.81NGYY36 pKa = 9.09QEE38 pKa = 4.02QSGTFTTFASLEE50 pKa = 4.26SGTEE54 pKa = 4.16SEE56 pKa = 5.51SSLDD60 pKa = 4.08DD61 pKa = 3.96HH62 pKa = 8.45DD63 pKa = 4.69EE64 pKa = 4.48DD65 pKa = 5.49SDD67 pKa = 4.06FFLSQEE73 pKa = 4.46FEE75 pKa = 4.43SEE77 pKa = 4.25LQFLEE82 pKa = 4.76SEE84 pKa = 4.62GSNDD88 pKa = 5.21DD89 pKa = 4.94DD90 pKa = 6.9DD91 pKa = 7.01DD92 pKa = 6.07DD93 pKa = 5.56EE94 pKa = 5.29EE95 pKa = 4.45MEE97 pKa = 4.31EE98 pKa = 5.15DD99 pKa = 4.39EE100 pKa = 5.5IDD102 pKa = 3.81VDD104 pKa = 3.66EE105 pKa = 4.51LTYY108 pKa = 11.18EE109 pKa = 4.08EE110 pKa = 5.6LIEE113 pKa = 4.68LGDD116 pKa = 3.8FIGRR120 pKa = 11.84EE121 pKa = 4.02KK122 pKa = 10.75RR123 pKa = 11.84GLSGSEE129 pKa = 3.57ICSCLHH135 pKa = 6.36SYY137 pKa = 10.46SFHH140 pKa = 6.04SAEE143 pKa = 4.43SKK145 pKa = 10.82SGVDD149 pKa = 3.3LCVICQVEE157 pKa = 4.35YY158 pKa = 11.2EE159 pKa = 4.13EE160 pKa = 6.23GEE162 pKa = 4.04ALVAIQCEE170 pKa = 4.57HH171 pKa = 7.54PYY173 pKa = 8.9HH174 pKa = 6.63TDD176 pKa = 4.64CISKK180 pKa = 9.12WLQIKK185 pKa = 9.1KK186 pKa = 9.74VCPICNTEE194 pKa = 3.21ISAPKK199 pKa = 9.88VVSS202 pKa = 3.42

Molecular weight: 22.64 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1S3ULA6|A0A1S3ULA6_VIGRR putative F-box protein At3g16210 OS=Vigna radiata var. radiata OX=3916 GN=LOC106766574 PE=4 SV=1
MM1 pKa = 7.32GNLWFRR7 pKa = 11.84SRR9 pKa = 11.84ICPVLLSTTARR20 pKa = 11.84CCSPPAVTCSLRR32 pKa = 11.84PPSPTTFSGHH42 pKa = 5.96RR43 pKa = 11.84LRR45 pKa = 11.84PPSPAPSPATVSGRR59 pKa = 11.84RR60 pKa = 11.84LRR62 pKa = 11.84PPSPTTVSGRR72 pKa = 11.84RR73 pKa = 11.84LRR75 pKa = 11.84PPSQATAAGTISGHH89 pKa = 5.56HH90 pKa = 6.52LRR92 pKa = 11.84LSSPATVFGHH102 pKa = 5.94RR103 pKa = 11.84RR104 pKa = 11.84RR105 pKa = 11.84HH106 pKa = 5.5RR107 pKa = 11.84LQPPSLAVISGHH119 pKa = 5.75RR120 pKa = 11.84LRR122 pKa = 11.84PSSPTVVSGHH132 pKa = 5.98RR133 pKa = 11.84LRR135 pKa = 11.84PLSPAPPPATVSSRR149 pKa = 11.84HH150 pKa = 5.08FRR152 pKa = 11.84RR153 pKa = 11.84SSS155 pKa = 2.81

Molecular weight: 16.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

26968

8067

35035

16406447

29

5502

468.3

52.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.398 ± 0.011

1.888 ± 0.007

5.303 ± 0.009

6.554 ± 0.018

4.254 ± 0.009

6.333 ± 0.015

2.515 ± 0.006

5.348 ± 0.01

6.186 ± 0.013

9.768 ± 0.017

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.38 ± 0.005

4.639 ± 0.011

4.788 ± 0.014

3.749 ± 0.009

5.22 ± 0.011

9.044 ± 0.015

4.934 ± 0.008

6.592 ± 0.009

1.266 ± 0.004

2.805 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski