Amino acid dipepetide frequency for Lactococcus phage 51701

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
0.923AlaAla: 0.923 ± 0.379
0.103AlaCys: 0.103 ± 0.107
2.975AlaAsp: 2.975 ± 0.568
4.719AlaGlu: 4.719 ± 0.676
4.309AlaPhe: 4.309 ± 0.72
4.001AlaGly: 4.001 ± 0.705
0.821AlaHis: 0.821 ± 0.328
4.514AlaIle: 4.514 ± 1.07
5.848AlaLys: 5.848 ± 0.843
5.54AlaLeu: 5.54 ± 0.862
2.667AlaMet: 2.667 ± 0.587
4.514AlaAsn: 4.514 ± 0.926
0.923AlaPro: 0.923 ± 0.267
2.565AlaGln: 2.565 ± 0.634
1.949AlaArg: 1.949 ± 0.441
3.591AlaSer: 3.591 ± 0.767
4.822AlaThr: 4.822 ± 0.884
3.693AlaVal: 3.693 ± 0.849
1.642AlaTrp: 1.642 ± 0.506
2.052AlaTyr: 2.052 ± 0.363
0.0AlaXaa: 0.0 ± 0.0
Cys
0.308CysAla: 0.308 ± 0.182
0.0CysCys: 0.0 ± 0.0
0.205CysAsp: 0.205 ± 0.151
0.205CysGlu: 0.205 ± 0.129
0.103CysPhe: 0.103 ± 0.114
0.821CysGly: 0.821 ± 0.318
0.103CysHis: 0.103 ± 0.094
0.41CysIle: 0.41 ± 0.217
0.923CysLys: 0.923 ± 0.352
0.513CysLeu: 0.513 ± 0.222
0.0CysMet: 0.0 ± 0.0
0.513CysAsn: 0.513 ± 0.234
0.205CysPro: 0.205 ± 0.141
0.308CysGln: 0.308 ± 0.183
0.41CysArg: 0.41 ± 0.231
0.205CysSer: 0.205 ± 0.138
0.0CysThr: 0.0 ± 0.0
0.308CysVal: 0.308 ± 0.159
0.205CysTrp: 0.205 ± 0.144
0.41CysTyr: 0.41 ± 0.301
0.0CysXaa: 0.0 ± 0.0
Asp
1.334AspAla: 1.334 ± 0.402
0.308AspCys: 0.308 ± 0.167
2.975AspAsp: 2.975 ± 0.567
3.899AspGlu: 3.899 ± 0.788
4.001AspPhe: 4.001 ± 0.531
4.514AspGly: 4.514 ± 0.764
0.923AspHis: 0.923 ± 0.281
4.206AspIle: 4.206 ± 0.789
6.053AspLys: 6.053 ± 0.642
5.643AspLeu: 5.643 ± 0.79
0.923AspMet: 0.923 ± 0.301
3.899AspAsn: 3.899 ± 0.773
1.539AspPro: 1.539 ± 0.4
0.513AspGln: 0.513 ± 0.223
1.744AspArg: 1.744 ± 0.467
2.257AspSer: 2.257 ± 0.547
3.693AspThr: 3.693 ± 0.587
3.283AspVal: 3.283 ± 0.612
0.718AspTrp: 0.718 ± 0.284
2.873AspTyr: 2.873 ± 0.557
0.0AspXaa: 0.0 ± 0.0
Glu
3.693GluAla: 3.693 ± 0.481
0.308GluCys: 0.308 ± 0.164
2.975GluAsp: 2.975 ± 0.61
4.719GluGlu: 4.719 ± 1.136
3.796GluPhe: 3.796 ± 0.529
2.052GluGly: 2.052 ± 0.448
1.129GluHis: 1.129 ± 0.363
5.54GluIle: 5.54 ± 0.719
6.156GluLys: 6.156 ± 1.141
9.131GluLeu: 9.131 ± 1.372
2.667GluMet: 2.667 ± 0.401
5.54GluAsn: 5.54 ± 0.817
1.129GluPro: 1.129 ± 0.403
4.206GluGln: 4.206 ± 0.816
3.283GluArg: 3.283 ± 0.651
4.104GluSer: 4.104 ± 0.537
3.693GluThr: 3.693 ± 0.676
4.309GluVal: 4.309 ± 0.624
0.923GluTrp: 0.923 ± 0.271
2.975GluTyr: 2.975 ± 0.644
0.0GluXaa: 0.0 ± 0.0
Phe
2.77PheAla: 2.77 ± 0.71
0.205PheCys: 0.205 ± 0.14
3.283PheAsp: 3.283 ± 0.545
2.36PheGlu: 2.36 ± 0.474
2.155PhePhe: 2.155 ± 0.863
2.667PheGly: 2.667 ± 0.625
0.513PheHis: 0.513 ± 0.289
3.18PheIle: 3.18 ± 0.581
4.309PheLys: 4.309 ± 0.676
2.462PheLeu: 2.462 ± 0.42
1.026PheMet: 1.026 ± 0.285
3.591PheAsn: 3.591 ± 0.698
0.513PhePro: 0.513 ± 0.221
1.334PheGln: 1.334 ± 0.331
1.744PheArg: 1.744 ± 0.365
4.309PheSer: 4.309 ± 0.902
3.488PheThr: 3.488 ± 0.512
2.155PheVal: 2.155 ± 0.409
0.41PheTrp: 0.41 ± 0.196
1.744PheTyr: 1.744 ± 0.493
0.0PheXaa: 0.0 ± 0.0
Gly
3.899GlyAla: 3.899 ± 0.783
0.513GlyCys: 0.513 ± 0.236
3.078GlyAsp: 3.078 ± 0.604
4.104GlyGlu: 4.104 ± 0.656
2.462GlyPhe: 2.462 ± 0.545
4.206GlyGly: 4.206 ± 0.866
0.821GlyHis: 0.821 ± 0.295
3.796GlyIle: 3.796 ± 0.946
6.156GlyLys: 6.156 ± 0.787
5.951GlyLeu: 5.951 ± 1.182
1.129GlyMet: 1.129 ± 0.404
3.899GlyAsn: 3.899 ± 0.572
0.205GlyPro: 0.205 ± 0.153
2.257GlyGln: 2.257 ± 0.431
1.642GlyArg: 1.642 ± 0.295
4.514GlySer: 4.514 ± 0.727
4.206GlyThr: 4.206 ± 0.73
6.156GlyVal: 6.156 ± 1.361
1.231GlyTrp: 1.231 ± 0.336
3.18GlyTyr: 3.18 ± 0.517
0.0GlyXaa: 0.0 ± 0.0
His
0.821HisAla: 0.821 ± 0.265
0.308HisCys: 0.308 ± 0.195
1.129HisAsp: 1.129 ± 0.309
0.616HisGlu: 0.616 ± 0.287
0.308HisPhe: 0.308 ± 0.176
1.334HisGly: 1.334 ± 0.34
0.0HisHis: 0.0 ± 0.0
1.231HisIle: 1.231 ± 0.411
0.718HisLys: 0.718 ± 0.259
0.821HisLeu: 0.821 ± 0.27
0.0HisMet: 0.0 ± 0.0
1.231HisAsn: 1.231 ± 0.404
0.308HisPro: 0.308 ± 0.139
0.308HisGln: 0.308 ± 0.184
0.718HisArg: 0.718 ± 0.27
0.205HisSer: 0.205 ± 0.209
1.026HisThr: 1.026 ± 0.304
0.718HisVal: 0.718 ± 0.283
0.103HisTrp: 0.103 ± 0.098
0.41HisTyr: 0.41 ± 0.219
0.0HisXaa: 0.0 ± 0.0
Ile
4.001IleAla: 4.001 ± 0.511
0.41IleCys: 0.41 ± 0.194
4.412IleAsp: 4.412 ± 0.606
6.156IleGlu: 6.156 ± 0.922
2.873IlePhe: 2.873 ± 0.59
4.309IleGly: 4.309 ± 0.71
0.718IleHis: 0.718 ± 0.24
5.13IleIle: 5.13 ± 0.833
6.771IleLys: 6.771 ± 0.733
5.438IleLeu: 5.438 ± 0.782
1.847IleMet: 1.847 ± 0.512
4.617IleAsn: 4.617 ± 0.484
1.744IlePro: 1.744 ± 0.463
1.949IleGln: 1.949 ± 0.419
1.334IleArg: 1.334 ± 0.371
4.514IleSer: 4.514 ± 0.945
5.232IleThr: 5.232 ± 0.742
4.206IleVal: 4.206 ± 0.752
1.026IleTrp: 1.026 ± 0.408
2.667IleTyr: 2.667 ± 0.428
0.0IleXaa: 0.0 ± 0.0
Lys
7.387LysAla: 7.387 ± 1.137
0.308LysCys: 0.308 ± 0.157
4.822LysAsp: 4.822 ± 0.663
9.131LysGlu: 9.131 ± 1.642
2.155LysPhe: 2.155 ± 0.544
5.951LysGly: 5.951 ± 1.035
1.026LysHis: 1.026 ± 0.358
6.156LysIle: 6.156 ± 0.804
7.797LysLys: 7.797 ± 1.09
7.695LysLeu: 7.695 ± 0.846
2.77LysMet: 2.77 ± 0.453
5.643LysAsn: 5.643 ± 0.795
2.155LysPro: 2.155 ± 0.525
3.488LysGln: 3.488 ± 0.573
3.899LysArg: 3.899 ± 0.661
5.027LysSer: 5.027 ± 0.76
5.745LysThr: 5.745 ± 0.872
5.438LysVal: 5.438 ± 0.635
1.436LysTrp: 1.436 ± 0.333
3.693LysTyr: 3.693 ± 0.751
0.0LysXaa: 0.0 ± 0.0
Leu
5.027LeuAla: 5.027 ± 0.7
0.103LeuCys: 0.103 ± 0.094
5.13LeuAsp: 5.13 ± 0.682
5.027LeuGlu: 5.027 ± 0.749
3.591LeuPhe: 3.591 ± 0.636
4.514LeuGly: 4.514 ± 0.878
1.334LeuHis: 1.334 ± 0.362
7.079LeuIle: 7.079 ± 0.933
8.105LeuLys: 8.105 ± 0.862
6.156LeuLeu: 6.156 ± 1.025
1.436LeuMet: 1.436 ± 0.326
5.232LeuAsn: 5.232 ± 0.913
3.283LeuPro: 3.283 ± 0.556
3.283LeuGln: 3.283 ± 0.513
2.155LeuArg: 2.155 ± 0.492
4.822LeuSer: 4.822 ± 0.672
5.951LeuThr: 5.951 ± 0.741
5.54LeuVal: 5.54 ± 0.606
1.436LeuTrp: 1.436 ± 0.376
4.822LeuTyr: 4.822 ± 0.788
0.0LeuXaa: 0.0 ± 0.0
Met
2.36MetAla: 2.36 ± 0.45
0.103MetCys: 0.103 ± 0.101
1.129MetAsp: 1.129 ± 0.281
1.949MetGlu: 1.949 ± 0.503
1.026MetPhe: 1.026 ± 0.342
0.821MetGly: 0.821 ± 0.256
0.308MetHis: 0.308 ± 0.188
2.052MetIle: 2.052 ± 0.449
2.462MetLys: 2.462 ± 0.526
1.436MetLeu: 1.436 ± 0.37
0.41MetMet: 0.41 ± 0.195
1.847MetAsn: 1.847 ± 0.395
0.718MetPro: 0.718 ± 0.328
1.642MetGln: 1.642 ± 0.349
0.616MetArg: 0.616 ± 0.251
1.539MetSer: 1.539 ± 0.359
1.539MetThr: 1.539 ± 0.386
1.744MetVal: 1.744 ± 0.298
0.205MetTrp: 0.205 ± 0.159
1.026MetTyr: 1.026 ± 0.336
0.0MetXaa: 0.0 ± 0.0
Asn
4.617AsnAla: 4.617 ± 0.85
0.205AsnCys: 0.205 ± 0.14
3.899AsnAsp: 3.899 ± 0.672
5.232AsnGlu: 5.232 ± 0.851
1.949AsnPhe: 1.949 ± 0.47
6.361AsnGly: 6.361 ± 0.686
0.718AsnHis: 0.718 ± 0.249
4.001AsnIle: 4.001 ± 0.624
5.745AsnLys: 5.745 ± 1.148
6.464AsnLeu: 6.464 ± 0.984
1.334AsnMet: 1.334 ± 0.378
4.206AsnAsn: 4.206 ± 0.699
2.257AsnPro: 2.257 ± 0.472
2.155AsnGln: 2.155 ± 0.496
1.847AsnArg: 1.847 ± 0.452
5.438AsnSer: 5.438 ± 0.816
4.412AsnThr: 4.412 ± 0.691
3.693AsnVal: 3.693 ± 0.576
1.334AsnTrp: 1.334 ± 0.337
2.155AsnTyr: 2.155 ± 0.589
0.0AsnXaa: 0.0 ± 0.0
Pro
1.642ProAla: 1.642 ± 0.442
0.103ProCys: 0.103 ± 0.113
1.231ProAsp: 1.231 ± 0.367
2.052ProGlu: 2.052 ± 0.512
0.821ProPhe: 0.821 ± 0.26
0.205ProGly: 0.205 ± 0.131
0.103ProHis: 0.103 ± 0.102
1.744ProIle: 1.744 ± 0.389
2.667ProLys: 2.667 ± 0.459
1.642ProLeu: 1.642 ± 0.377
0.718ProMet: 0.718 ± 0.246
2.257ProAsn: 2.257 ± 0.828
0.616ProPro: 0.616 ± 0.296
0.616ProGln: 0.616 ± 0.228
0.41ProArg: 0.41 ± 0.219
1.026ProSer: 1.026 ± 0.39
2.565ProThr: 2.565 ± 0.444
1.231ProVal: 1.231 ± 0.374
0.205ProTrp: 0.205 ± 0.144
0.923ProTyr: 0.923 ± 0.306
0.0ProXaa: 0.0 ± 0.0
Gln
2.975GlnAla: 2.975 ± 0.582
0.205GlnCys: 0.205 ± 0.151
1.744GlnAsp: 1.744 ± 0.393
2.36GlnGlu: 2.36 ± 0.482
1.129GlnPhe: 1.129 ± 0.345
2.667GlnGly: 2.667 ± 0.476
0.513GlnHis: 0.513 ± 0.202
1.231GlnIle: 1.231 ± 0.256
2.77GlnLys: 2.77 ± 0.524
3.18GlnLeu: 3.18 ± 0.616
1.026GlnMet: 1.026 ± 0.266
1.949GlnAsn: 1.949 ± 0.473
0.821GlnPro: 0.821 ± 0.209
1.436GlnGln: 1.436 ± 0.399
1.847GlnArg: 1.847 ± 0.49
2.257GlnSer: 2.257 ± 0.383
2.77GlnThr: 2.77 ± 0.507
2.462GlnVal: 2.462 ± 0.495
0.513GlnTrp: 0.513 ± 0.199
1.231GlnTyr: 1.231 ± 0.308
0.0GlnXaa: 0.0 ± 0.0
Arg
2.565ArgAla: 2.565 ± 0.686
0.513ArgCys: 0.513 ± 0.203
1.436ArgAsp: 1.436 ± 0.366
2.155ArgGlu: 2.155 ± 0.492
1.129ArgPhe: 1.129 ± 0.293
1.949ArgGly: 1.949 ± 0.48
0.616ArgHis: 0.616 ± 0.259
1.847ArgIle: 1.847 ± 0.461
3.796ArgLys: 3.796 ± 0.667
3.078ArgLeu: 3.078 ± 0.617
0.821ArgMet: 0.821 ± 0.288
2.155ArgAsn: 2.155 ± 0.485
0.616ArgPro: 0.616 ± 0.231
1.231ArgGln: 1.231 ± 0.324
1.744ArgArg: 1.744 ± 0.387
1.847ArgSer: 1.847 ± 0.473
1.539ArgThr: 1.539 ± 0.355
2.052ArgVal: 2.052 ± 0.375
0.308ArgTrp: 0.308 ± 0.208
1.847ArgTyr: 1.847 ± 0.475
0.0ArgXaa: 0.0 ± 0.0
Ser
5.745SerAla: 5.745 ± 1.61
0.718SerCys: 0.718 ± 0.348
4.412SerAsp: 4.412 ± 0.673
3.693SerGlu: 3.693 ± 0.661
3.18SerPhe: 3.18 ± 0.623
5.745SerGly: 5.745 ± 1.486
0.616SerHis: 0.616 ± 0.232
3.796SerIle: 3.796 ± 0.497
5.027SerLys: 5.027 ± 0.76
5.335SerLeu: 5.335 ± 0.831
1.744SerMet: 1.744 ± 0.412
3.283SerAsn: 3.283 ± 0.75
1.539SerPro: 1.539 ± 0.369
1.744SerGln: 1.744 ± 0.491
1.949SerArg: 1.949 ± 0.343
6.053SerSer: 6.053 ± 1.223
3.283SerThr: 3.283 ± 0.803
3.899SerVal: 3.899 ± 0.654
1.026SerTrp: 1.026 ± 0.393
2.462SerTyr: 2.462 ± 0.682
0.0SerXaa: 0.0 ± 0.0
Thr
5.13ThrAla: 5.13 ± 0.665
0.205ThrCys: 0.205 ± 0.148
3.488ThrAsp: 3.488 ± 0.588
5.027ThrGlu: 5.027 ± 0.615
3.18ThrPhe: 3.18 ± 0.525
4.309ThrGly: 4.309 ± 0.671
0.308ThrHis: 0.308 ± 0.172
4.925ThrIle: 4.925 ± 0.848
5.438ThrLys: 5.438 ± 0.692
5.745ThrLeu: 5.745 ± 0.76
1.129ThrMet: 1.129 ± 0.307
5.13ThrAsn: 5.13 ± 0.656
1.744ThrPro: 1.744 ± 0.382
2.462ThrGln: 2.462 ± 0.539
2.155ThrArg: 2.155 ± 0.546
4.617ThrSer: 4.617 ± 0.747
4.104ThrThr: 4.104 ± 0.648
5.438ThrVal: 5.438 ± 0.939
1.334ThrTrp: 1.334 ± 0.38
2.257ThrTyr: 2.257 ± 0.54
0.0ThrXaa: 0.0 ± 0.0
Val
4.001ValAla: 4.001 ± 0.553
0.616ValCys: 0.616 ± 0.262
3.899ValAsp: 3.899 ± 0.64
4.412ValGlu: 4.412 ± 0.506
3.283ValPhe: 3.283 ± 0.567
2.975ValGly: 2.975 ± 0.499
0.513ValHis: 0.513 ± 0.214
4.206ValIle: 4.206 ± 0.735
6.669ValLys: 6.669 ± 0.645
3.591ValLeu: 3.591 ± 0.572
1.744ValMet: 1.744 ± 0.379
3.18ValAsn: 3.18 ± 0.619
1.334ValPro: 1.334 ± 0.361
1.744ValGln: 1.744 ± 0.373
2.257ValArg: 2.257 ± 0.627
5.643ValSer: 5.643 ± 0.984
5.951ValThr: 5.951 ± 0.802
3.899ValVal: 3.899 ± 1.238
0.718ValTrp: 0.718 ± 0.355
2.77ValTyr: 2.77 ± 0.617
0.0ValXaa: 0.0 ± 0.0
Trp
1.436TrpAla: 1.436 ± 0.432
0.205TrpCys: 0.205 ± 0.133
0.718TrpAsp: 0.718 ± 0.263
0.923TrpGlu: 0.923 ± 0.281
0.821TrpPhe: 0.821 ± 0.363
1.129TrpGly: 1.129 ± 0.435
0.41TrpHis: 0.41 ± 0.205
0.923TrpIle: 0.923 ± 0.33
0.923TrpLys: 0.923 ± 0.303
1.129TrpLeu: 1.129 ± 0.364
0.513TrpMet: 0.513 ± 0.206
1.847TrpAsn: 1.847 ± 0.507
0.103TrpPro: 0.103 ± 0.102
0.718TrpGln: 0.718 ± 0.235
0.513TrpArg: 0.513 ± 0.282
1.026TrpSer: 1.026 ± 0.297
0.513TrpThr: 0.513 ± 0.23
0.41TrpVal: 0.41 ± 0.213
0.0TrpTrp: 0.0 ± 0.0
0.923TrpTyr: 0.923 ± 0.259
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.539TyrAla: 1.539 ± 0.483
0.718TyrCys: 0.718 ± 0.387
2.36TyrAsp: 2.36 ± 0.624
3.591TyrGlu: 3.591 ± 0.727
1.949TyrPhe: 1.949 ± 0.457
2.873TyrGly: 2.873 ± 0.549
0.821TyrHis: 0.821 ± 0.249
3.386TyrIle: 3.386 ± 0.738
3.386TyrLys: 3.386 ± 0.737
3.078TyrLeu: 3.078 ± 0.664
0.923TyrMet: 0.923 ± 0.406
3.591TyrAsn: 3.591 ± 0.487
1.026TyrPro: 1.026 ± 0.406
1.129TyrGln: 1.129 ± 0.383
1.026TyrArg: 1.026 ± 0.269
2.257TyrSer: 2.257 ± 0.524
3.591TyrThr: 3.591 ± 0.876
2.77TyrVal: 2.77 ± 0.467
0.41TyrTrp: 0.41 ± 0.211
2.565TyrTyr: 2.565 ± 0.635
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 55 proteins (9748 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski