Amino acid dipepetide frequency for Sinomonas sp. R1AF57

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
23.733AlaAla: 23.733 ± 0.243
0.858AlaCys: 0.858 ± 0.029
7.689AlaAsp: 7.689 ± 0.088
9.155AlaGlu: 9.155 ± 0.117
4.178AlaPhe: 4.178 ± 0.058
14.111AlaGly: 14.111 ± 0.163
2.772AlaHis: 2.772 ± 0.057
4.776AlaIle: 4.776 ± 0.079
3.306AlaLys: 3.306 ± 0.068
14.491AlaLeu: 14.491 ± 0.155
2.681AlaMet: 2.681 ± 0.045
2.395AlaAsn: 2.395 ± 0.047
7.282AlaPro: 7.282 ± 0.107
4.355AlaGln: 4.355 ± 0.078
9.697AlaArg: 9.697 ± 0.134
7.622AlaSer: 7.622 ± 0.088
6.537AlaThr: 6.537 ± 0.081
12.913AlaVal: 12.913 ± 0.129
1.936AlaTrp: 1.936 ± 0.046
2.573AlaTyr: 2.573 ± 0.055
0.0AlaXaa: 0.0 ± 0.0
Cys
0.771CysAla: 0.771 ± 0.025
0.067CysCys: 0.067 ± 0.009
0.327CysAsp: 0.327 ± 0.019
0.303CysGlu: 0.303 ± 0.018
0.208CysPhe: 0.208 ± 0.014
0.727CysGly: 0.727 ± 0.025
0.142CysHis: 0.142 ± 0.01
0.204CysIle: 0.204 ± 0.016
0.075CysLys: 0.075 ± 0.009
0.526CysLeu: 0.526 ± 0.022
0.089CysMet: 0.089 ± 0.009
0.115CysAsn: 0.115 ± 0.01
0.357CysPro: 0.357 ± 0.023
0.15CysGln: 0.15 ± 0.009
0.472CysArg: 0.472 ± 0.02
0.398CysSer: 0.398 ± 0.019
0.386CysThr: 0.386 ± 0.021
0.439CysVal: 0.439 ± 0.019
0.081CysTrp: 0.081 ± 0.009
0.129CysTyr: 0.129 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
8.117AspAla: 8.117 ± 0.108
0.275AspCys: 0.275 ± 0.016
3.1AspAsp: 3.1 ± 0.063
3.661AspGlu: 3.661 ± 0.069
1.748AspPhe: 1.748 ± 0.041
5.984AspGly: 5.984 ± 0.095
1.21AspHis: 1.21 ± 0.03
1.996AspIle: 1.996 ± 0.044
1.023AspLys: 1.023 ± 0.039
5.668AspLeu: 5.668 ± 0.067
0.815AspMet: 0.815 ± 0.027
0.827AspAsn: 0.827 ± 0.027
3.851AspPro: 3.851 ± 0.059
1.422AspGln: 1.422 ± 0.032
3.918AspArg: 3.918 ± 0.065
2.56AspSer: 2.56 ± 0.048
2.501AspThr: 2.501 ± 0.047
4.823AspVal: 4.823 ± 0.069
0.849AspTrp: 0.849 ± 0.031
1.25AspTyr: 1.25 ± 0.029
0.0AspXaa: 0.0 ± 0.0
Glu
8.295GluAla: 8.295 ± 0.116
0.299GluCys: 0.299 ± 0.016
3.2GluAsp: 3.2 ± 0.063
3.426GluGlu: 3.426 ± 0.064
1.651GluPhe: 1.651 ± 0.037
4.692GluGly: 4.692 ± 0.069
1.597GluHis: 1.597 ± 0.042
2.352GluIle: 2.352 ± 0.056
1.568GluLys: 1.568 ± 0.045
6.508GluLeu: 6.508 ± 0.091
0.894GluMet: 0.894 ± 0.029
1.15GluAsn: 1.15 ± 0.036
3.086GluPro: 3.086 ± 0.058
2.086GluGln: 2.086 ± 0.049
5.098GluArg: 5.098 ± 0.084
2.719GluSer: 2.719 ± 0.055
2.854GluThr: 2.854 ± 0.05
4.643GluVal: 4.643 ± 0.071
0.866GluTrp: 0.866 ± 0.028
1.15GluTyr: 1.15 ± 0.034
0.0GluXaa: 0.0 ± 0.0
Phe
4.376PheAla: 4.376 ± 0.071
0.224PheCys: 0.224 ± 0.015
2.014PheAsp: 2.014 ± 0.044
1.689PheGlu: 1.689 ± 0.042
1.066PhePhe: 1.066 ± 0.037
3.526PheGly: 3.526 ± 0.061
0.622PheHis: 0.622 ± 0.025
1.06PheIle: 1.06 ± 0.03
0.539PheLys: 0.539 ± 0.025
2.906PheLeu: 2.906 ± 0.063
0.51PheMet: 0.51 ± 0.02
0.699PheAsn: 0.699 ± 0.028
1.484PhePro: 1.484 ± 0.035
0.754PheGln: 0.754 ± 0.026
1.89PheArg: 1.89 ± 0.04
1.813PheSer: 1.813 ± 0.042
1.978PheThr: 1.978 ± 0.046
2.57PheVal: 2.57 ± 0.046
0.445PheTrp: 0.445 ± 0.022
0.678PheTyr: 0.678 ± 0.028
0.0PheXaa: 0.0 ± 0.0
Gly
12.15GlyAla: 12.15 ± 0.148
0.622GlyCys: 0.622 ± 0.026
4.647GlyAsp: 4.647 ± 0.071
5.015GlyGlu: 5.015 ± 0.08
3.241GlyPhe: 3.241 ± 0.055
8.577GlyGly: 8.577 ± 0.108
2.144GlyHis: 2.144 ± 0.051
4.29GlyIle: 4.29 ± 0.063
2.46GlyLys: 2.46 ± 0.061
9.772GlyLeu: 9.772 ± 0.119
2.05GlyMet: 2.05 ± 0.043
1.796GlyAsn: 1.796 ± 0.046
4.788GlyPro: 4.788 ± 0.07
2.968GlyGln: 2.968 ± 0.055
7.289GlyArg: 7.289 ± 0.087
5.83GlySer: 5.83 ± 0.084
6.094GlyThr: 6.094 ± 0.092
7.888GlyVal: 7.888 ± 0.085
1.777GlyTrp: 1.777 ± 0.048
2.319GlyTyr: 2.319 ± 0.06
0.0GlyXaa: 0.0 ± 0.0
His
2.638HisAla: 2.638 ± 0.057
0.166HisCys: 0.166 ± 0.012
1.395HisAsp: 1.395 ± 0.037
1.317HisGlu: 1.317 ± 0.035
0.604HisPhe: 0.604 ± 0.021
2.249HisGly: 2.249 ± 0.052
0.596HisHis: 0.596 ± 0.028
0.693HisIle: 0.693 ± 0.026
0.299HisLys: 0.299 ± 0.015
2.104HisLeu: 2.104 ± 0.047
0.363HisMet: 0.363 ± 0.017
0.348HisAsn: 0.348 ± 0.018
1.556HisPro: 1.556 ± 0.035
0.522HisGln: 0.522 ± 0.024
1.773HisArg: 1.773 ± 0.037
1.049HisSer: 1.049 ± 0.026
1.069HisThr: 1.069 ± 0.029
1.715HisVal: 1.715 ± 0.04
0.345HisTrp: 0.345 ± 0.019
0.424HisTyr: 0.424 ± 0.018
0.0HisXaa: 0.0 ± 0.0
Ile
5.711IleAla: 5.711 ± 0.083
0.243IleCys: 0.243 ± 0.013
2.303IleAsp: 2.303 ± 0.055
2.397IleGlu: 2.397 ± 0.053
1.01IlePhe: 1.01 ± 0.034
3.983IleGly: 3.983 ± 0.065
0.666IleHis: 0.666 ± 0.025
1.351IleIle: 1.351 ± 0.038
0.811IleLys: 0.811 ± 0.028
3.448IleLeu: 3.448 ± 0.056
0.638IleMet: 0.638 ± 0.023
0.852IleAsn: 0.852 ± 0.034
2.164IlePro: 2.164 ± 0.042
0.955IleGln: 0.955 ± 0.03
2.385IleArg: 2.385 ± 0.042
1.928IleSer: 1.928 ± 0.041
2.244IleThr: 2.244 ± 0.044
3.611IleVal: 3.611 ± 0.064
0.398IleTrp: 0.398 ± 0.018
0.627IleTyr: 0.627 ± 0.025
0.0IleXaa: 0.0 ± 0.0
Lys
3.198LysAla: 3.198 ± 0.063
0.085LysCys: 0.085 ± 0.008
1.483LysAsp: 1.483 ± 0.045
1.221LysGlu: 1.221 ± 0.039
0.593LysPhe: 0.593 ± 0.024
1.856LysGly: 1.856 ± 0.049
0.476LysHis: 0.476 ± 0.02
0.953LysIle: 0.953 ± 0.031
0.748LysLys: 0.748 ± 0.036
1.987LysLeu: 1.987 ± 0.04
0.444LysMet: 0.444 ± 0.021
0.551LysAsn: 0.551 ± 0.023
1.287LysPro: 1.287 ± 0.035
0.64LysGln: 0.64 ± 0.028
1.461LysArg: 1.461 ± 0.04
1.129LysSer: 1.129 ± 0.037
1.274LysThr: 1.274 ± 0.041
2.048LysVal: 2.048 ± 0.044
0.229LysTrp: 0.229 ± 0.014
0.53LysTyr: 0.53 ± 0.022
0.0LysXaa: 0.0 ± 0.0
Leu
16.337LeuAla: 16.337 ± 0.182
0.617LeuCys: 0.617 ± 0.023
6.11LeuAsp: 6.11 ± 0.078
5.669LeuGlu: 5.669 ± 0.084
2.805LeuPhe: 2.805 ± 0.059
10.063LeuGly: 10.063 ± 0.116
1.937LeuHis: 1.937 ± 0.038
3.526LeuIle: 3.526 ± 0.065
2.097LeuLys: 2.097 ± 0.044
9.939LeuLeu: 9.939 ± 0.12
1.726LeuMet: 1.726 ± 0.038
1.898LeuAsn: 1.898 ± 0.046
5.758LeuPro: 5.758 ± 0.067
2.237LeuGln: 2.237 ± 0.044
7.436LeuArg: 7.436 ± 0.104
5.619LeuSer: 5.619 ± 0.067
5.975LeuThr: 5.975 ± 0.065
8.993LeuVal: 8.993 ± 0.117
1.283LeuTrp: 1.283 ± 0.039
1.719LeuTyr: 1.719 ± 0.039
0.0LeuXaa: 0.0 ± 0.0
Met
2.567MetAla: 2.567 ± 0.042
0.113MetCys: 0.113 ± 0.011
1.01MetAsp: 1.01 ± 0.031
0.785MetGlu: 0.785 ± 0.03
0.536MetPhe: 0.536 ± 0.024
1.57MetGly: 1.57 ± 0.039
0.351MetHis: 0.351 ± 0.017
0.673MetIle: 0.673 ± 0.025
0.489MetLys: 0.489 ± 0.024
1.695MetLeu: 1.695 ± 0.038
0.335MetMet: 0.335 ± 0.019
0.438MetAsn: 0.438 ± 0.019
1.074MetPro: 1.074 ± 0.037
0.451MetGln: 0.451 ± 0.02
1.219MetArg: 1.219 ± 0.033
1.373MetSer: 1.373 ± 0.033
1.39MetThr: 1.39 ± 0.039
1.525MetVal: 1.525 ± 0.039
0.221MetTrp: 0.221 ± 0.014
0.297MetTyr: 0.297 ± 0.018
0.0MetXaa: 0.0 ± 0.0
Asn
2.454AsnAla: 2.454 ± 0.055
0.133AsnCys: 0.133 ± 0.01
1.006AsnAsp: 1.006 ± 0.035
0.929AsnGlu: 0.929 ± 0.03
0.585AsnPhe: 0.585 ± 0.023
1.977AsnGly: 1.977 ± 0.051
0.404AsnHis: 0.404 ± 0.019
0.786AsnIle: 0.786 ± 0.031
0.405AsnLys: 0.405 ± 0.019
1.889AsnLeu: 1.889 ± 0.046
0.337AsnMet: 0.337 ± 0.017
0.465AsnAsn: 0.465 ± 0.021
1.575AsnPro: 1.575 ± 0.037
0.565AsnGln: 0.565 ± 0.023
1.296AsnArg: 1.296 ± 0.034
0.995AsnSer: 0.995 ± 0.035
1.057AsnThr: 1.057 ± 0.031
1.576AsnVal: 1.576 ± 0.037
0.296AsnTrp: 0.296 ± 0.014
0.473AsnTyr: 0.473 ± 0.018
0.0AsnXaa: 0.0 ± 0.0
Pro
8.433ProAla: 8.433 ± 0.112
0.245ProCys: 0.245 ± 0.015
3.681ProAsp: 3.681 ± 0.066
4.334ProGlu: 4.334 ± 0.071
1.713ProPhe: 1.713 ± 0.036
5.976ProGly: 5.976 ± 0.086
1.197ProHis: 1.197 ± 0.035
1.746ProIle: 1.746 ± 0.05
1.144ProLys: 1.144 ± 0.035
5.153ProLeu: 5.153 ± 0.067
0.892ProMet: 0.892 ± 0.03
1.03ProAsn: 1.03 ± 0.035
2.5ProPro: 2.5 ± 0.06
1.679ProGln: 1.679 ± 0.041
3.556ProArg: 3.556 ± 0.06
3.6ProSer: 3.6 ± 0.061
3.066ProThr: 3.066 ± 0.06
4.808ProVal: 4.808 ± 0.064
0.88ProTrp: 0.88 ± 0.027
1.102ProTyr: 1.102 ± 0.032
0.0ProXaa: 0.0 ± 0.0
Gln
3.796GlnAla: 3.796 ± 0.07
0.145GlnCys: 0.145 ± 0.012
1.377GlnAsp: 1.377 ± 0.035
1.425GlnGlu: 1.425 ± 0.038
0.846GlnPhe: 0.846 ± 0.026
2.286GlnGly: 2.286 ± 0.061
0.667GlnHis: 0.667 ± 0.023
1.289GlnIle: 1.289 ± 0.035
0.738GlnLys: 0.738 ± 0.03
3.273GlnLeu: 3.273 ± 0.064
0.536GlnMet: 0.536 ± 0.02
0.624GlnAsn: 0.624 ± 0.025
1.649GlnPro: 1.649 ± 0.045
1.164GlnGln: 1.164 ± 0.042
2.33GlnArg: 2.33 ± 0.044
1.401GlnSer: 1.401 ± 0.035
1.504GlnThr: 1.504 ± 0.044
2.142GlnVal: 2.142 ± 0.043
0.464GlnTrp: 0.464 ± 0.021
0.661GlnTyr: 0.661 ± 0.025
0.0GlnXaa: 0.0 ± 0.0
Arg
9.146ArgAla: 9.146 ± 0.111
0.414ArgCys: 0.414 ± 0.022
3.824ArgAsp: 3.824 ± 0.056
4.532ArgGlu: 4.532 ± 0.067
2.337ArgPhe: 2.337 ± 0.048
5.888ArgGly: 5.888 ± 0.088
1.731ArgHis: 1.731 ± 0.037
3.257ArgIle: 3.257 ± 0.054
1.499ArgLys: 1.499 ± 0.037
7.979ArgLeu: 7.979 ± 0.098
1.503ArgMet: 1.503 ± 0.034
1.307ArgAsn: 1.307 ± 0.037
4.101ArgPro: 4.101 ± 0.072
2.101ArgGln: 2.101 ± 0.038
7.008ArgArg: 7.008 ± 0.121
3.979ArgSer: 3.979 ± 0.06
4.105ArgThr: 4.105 ± 0.06
5.575ArgVal: 5.575 ± 0.072
1.214ArgTrp: 1.214 ± 0.032
1.473ArgTyr: 1.473 ± 0.038
0.0ArgXaa: 0.0 ± 0.0
Ser
7.544SerAla: 7.544 ± 0.091
0.322SerCys: 0.322 ± 0.019
2.533SerAsp: 2.533 ± 0.05
2.742SerGlu: 2.742 ± 0.048
1.84SerPhe: 1.84 ± 0.044
6.035SerGly: 6.035 ± 0.08
1.087SerHis: 1.087 ± 0.029
2.086SerIle: 2.086 ± 0.039
1.247SerLys: 1.247 ± 0.037
5.416SerLeu: 5.416 ± 0.07
1.183SerMet: 1.183 ± 0.035
1.031SerAsn: 1.031 ± 0.032
3.464SerPro: 3.464 ± 0.058
1.489SerGln: 1.489 ± 0.04
3.824SerArg: 3.824 ± 0.064
3.513SerSer: 3.513 ± 0.073
3.191SerThr: 3.191 ± 0.063
4.531SerVal: 4.531 ± 0.056
0.86SerTrp: 0.86 ± 0.031
1.171SerTyr: 1.171 ± 0.035
0.0SerXaa: 0.0 ± 0.0
Thr
7.947ThrAla: 7.947 ± 0.099
0.325ThrCys: 0.325 ± 0.015
2.937ThrAsp: 2.937 ± 0.053
2.879ThrGlu: 2.879 ± 0.054
1.773ThrPhe: 1.773 ± 0.042
5.692ThrGly: 5.692 ± 0.074
1.143ThrHis: 1.143 ± 0.033
2.037ThrIle: 2.037 ± 0.044
1.203ThrLys: 1.203 ± 0.032
5.474ThrLeu: 5.474 ± 0.069
0.972ThrMet: 0.972 ± 0.028
1.022ThrAsn: 1.022 ± 0.032
3.693ThrPro: 3.693 ± 0.064
1.461ThrGln: 1.461 ± 0.04
3.264ThrArg: 3.264 ± 0.052
3.044ThrSer: 3.044 ± 0.058
3.294ThrThr: 3.294 ± 0.063
5.578ThrVal: 5.578 ± 0.075
0.766ThrTrp: 0.766 ± 0.03
1.129ThrTyr: 1.129 ± 0.037
0.0ThrXaa: 0.0 ± 0.0
Val
11.641ValAla: 11.641 ± 0.129
0.557ValCys: 0.557 ± 0.024
5.025ValAsp: 5.025 ± 0.059
4.847ValGlu: 4.847 ± 0.087
2.822ValPhe: 2.822 ± 0.054
7.356ValGly: 7.356 ± 0.081
1.796ValHis: 1.796 ± 0.043
3.29ValIle: 3.29 ± 0.064
1.808ValLys: 1.808 ± 0.048
9.58ValLeu: 9.58 ± 0.106
1.494ValMet: 1.494 ± 0.04
1.816ValAsn: 1.816 ± 0.05
5.202ValPro: 5.202 ± 0.078
2.216ValGln: 2.216 ± 0.047
6.253ValArg: 6.253 ± 0.08
4.542ValSer: 4.542 ± 0.063
5.08ValThr: 5.08 ± 0.072
8.834ValVal: 8.834 ± 0.107
1.162ValTrp: 1.162 ± 0.034
1.566ValTyr: 1.566 ± 0.035
0.0ValXaa: 0.0 ± 0.0
Trp
1.741TrpAla: 1.741 ± 0.048
0.106TrpCys: 0.106 ± 0.009
0.824TrpAsp: 0.824 ± 0.026
0.691TrpGlu: 0.691 ± 0.025
0.531TrpPhe: 0.531 ± 0.022
1.159TrpGly: 1.159 ± 0.03
0.333TrpHis: 0.333 ± 0.019
0.683TrpIle: 0.683 ± 0.024
0.357TrpLys: 0.357 ± 0.019
1.801TrpLeu: 1.801 ± 0.044
0.333TrpMet: 0.333 ± 0.018
0.389TrpAsn: 0.389 ± 0.019
0.723TrpPro: 0.723 ± 0.027
0.504TrpGln: 0.504 ± 0.018
1.177TrpArg: 1.177 ± 0.036
0.828TrpSer: 0.828 ± 0.03
0.852TrpThr: 0.852 ± 0.028
1.108TrpVal: 1.108 ± 0.033
0.329TrpTrp: 0.329 ± 0.018
0.282TrpTyr: 0.282 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.466TyrAla: 2.466 ± 0.054
0.147TyrCys: 0.147 ± 0.01
1.113TyrAsp: 1.113 ± 0.029
1.119TyrGlu: 1.119 ± 0.033
0.758TyrPhe: 0.758 ± 0.029
2.013TyrGly: 2.013 ± 0.048
0.354TyrHis: 0.354 ± 0.019
0.677TyrIle: 0.677 ± 0.027
0.374TyrLys: 0.374 ± 0.018
2.134TyrLeu: 2.134 ± 0.044
0.316TyrMet: 0.316 ± 0.018
0.45TyrAsn: 0.45 ± 0.021
1.14TyrPro: 1.14 ± 0.034
0.599TyrGln: 0.599 ± 0.025
1.624TyrArg: 1.624 ± 0.038
1.17TyrSer: 1.17 ± 0.037
1.182TyrThr: 1.182 ± 0.041
1.568TyrVal: 1.568 ± 0.038
0.35TyrTrp: 0.35 ± 0.02
0.529TyrTyr: 0.529 ± 0.022
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3510 proteins (1169589 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski