Amino acid dipepetide frequency for Microbacterium phage BubbaBear

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.595AlaAla: 13.595 ± 1.305
0.228AlaCys: 0.228 ± 0.136
7.823AlaAsp: 7.823 ± 0.595
6.683AlaGlu: 6.683 ± 0.83
3.721AlaPhe: 3.721 ± 0.437
10.633AlaGly: 10.633 ± 1.143
1.975AlaHis: 1.975 ± 0.417
6.456AlaIle: 6.456 ± 1.013
5.392AlaLys: 5.392 ± 0.852
9.493AlaLeu: 9.493 ± 1.164
2.43AlaMet: 2.43 ± 0.471
3.949AlaAsn: 3.949 ± 0.53
5.24AlaPro: 5.24 ± 0.578
5.772AlaGln: 5.772 ± 0.652
7.139AlaArg: 7.139 ± 0.92
5.392AlaSer: 5.392 ± 0.625
6.911AlaThr: 6.911 ± 1.131
8.658AlaVal: 8.658 ± 0.74
2.886AlaTrp: 2.886 ± 0.464
2.658AlaTyr: 2.658 ± 0.516
0.0AlaXaa: 0.0 ± 0.0
Cys
0.152CysAla: 0.152 ± 0.107
0.0CysCys: 0.0 ± 0.0
0.38CysAsp: 0.38 ± 0.171
0.076CysGlu: 0.076 ± 0.081
0.0CysPhe: 0.0 ± 0.0
0.759CysGly: 0.759 ± 0.304
0.152CysHis: 0.152 ± 0.123
0.076CysIle: 0.076 ± 0.077
0.076CysLys: 0.076 ± 0.079
0.152CysLeu: 0.152 ± 0.096
0.228CysMet: 0.228 ± 0.14
0.304CysAsn: 0.304 ± 0.159
0.911CysPro: 0.911 ± 0.345
0.152CysGln: 0.152 ± 0.109
0.38CysArg: 0.38 ± 0.205
0.304CysSer: 0.304 ± 0.153
0.228CysThr: 0.228 ± 0.144
0.228CysVal: 0.228 ± 0.125
0.0CysTrp: 0.0 ± 0.0
0.076CysTyr: 0.076 ± 0.1
0.0CysXaa: 0.0 ± 0.0
Asp
7.215AspAla: 7.215 ± 0.7
0.152AspCys: 0.152 ± 0.118
4.785AspAsp: 4.785 ± 0.87
3.266AspGlu: 3.266 ± 0.56
2.127AspPhe: 2.127 ± 0.361
6.304AspGly: 6.304 ± 0.636
1.747AspHis: 1.747 ± 0.39
3.418AspIle: 3.418 ± 0.467
1.747AspLys: 1.747 ± 0.354
6.304AspLeu: 6.304 ± 0.712
1.671AspMet: 1.671 ± 0.365
1.975AspAsn: 1.975 ± 0.347
4.481AspPro: 4.481 ± 0.571
2.354AspGln: 2.354 ± 0.365
3.114AspArg: 3.114 ± 0.605
3.645AspSer: 3.645 ± 0.446
3.494AspThr: 3.494 ± 0.512
3.873AspVal: 3.873 ± 0.63
1.595AspTrp: 1.595 ± 0.353
2.81AspTyr: 2.81 ± 0.424
0.0AspXaa: 0.0 ± 0.0
Glu
6.911GluAla: 6.911 ± 0.719
0.152GluCys: 0.152 ± 0.114
4.177GluAsp: 4.177 ± 0.689
4.177GluGlu: 4.177 ± 0.58
2.354GluPhe: 2.354 ± 0.362
3.949GluGly: 3.949 ± 0.658
1.975GluHis: 1.975 ± 0.426
0.911GluIle: 0.911 ± 0.237
2.127GluLys: 2.127 ± 0.396
7.063GluLeu: 7.063 ± 0.609
1.519GluMet: 1.519 ± 0.361
1.367GluAsn: 1.367 ± 0.365
3.038GluPro: 3.038 ± 0.529
2.43GluGln: 2.43 ± 0.374
2.962GluArg: 2.962 ± 0.508
2.506GluSer: 2.506 ± 0.428
3.418GluThr: 3.418 ± 0.472
6.076GluVal: 6.076 ± 0.704
1.519GluTrp: 1.519 ± 0.397
1.215GluTyr: 1.215 ± 0.264
0.0GluXaa: 0.0 ± 0.0
Phe
3.494PheAla: 3.494 ± 0.601
0.076PheCys: 0.076 ± 0.076
1.747PheAsp: 1.747 ± 0.331
2.658PheGlu: 2.658 ± 0.377
1.063PhePhe: 1.063 ± 0.303
2.658PheGly: 2.658 ± 0.449
0.152PheHis: 0.152 ± 0.115
1.671PheIle: 1.671 ± 0.402
1.899PheLys: 1.899 ± 0.309
2.354PheLeu: 2.354 ± 0.509
0.911PheMet: 0.911 ± 0.232
1.063PheAsn: 1.063 ± 0.27
1.443PhePro: 1.443 ± 0.304
0.835PheGln: 0.835 ± 0.283
3.342PheArg: 3.342 ± 0.592
1.823PheSer: 1.823 ± 0.32
2.354PheThr: 2.354 ± 0.435
1.519PheVal: 1.519 ± 0.337
0.532PheTrp: 0.532 ± 0.275
0.532PheTyr: 0.532 ± 0.168
0.0PheXaa: 0.0 ± 0.0
Gly
7.823GlyAla: 7.823 ± 0.781
0.759GlyCys: 0.759 ± 0.239
6.228GlyAsp: 6.228 ± 0.651
4.101GlyGlu: 4.101 ± 0.579
2.962GlyPhe: 2.962 ± 0.416
6.076GlyGly: 6.076 ± 1.048
1.443GlyHis: 1.443 ± 0.376
4.329GlyIle: 4.329 ± 0.588
3.645GlyLys: 3.645 ± 0.52
6.38GlyLeu: 6.38 ± 0.617
1.975GlyMet: 1.975 ± 0.422
2.81GlyAsn: 2.81 ± 0.596
3.114GlyPro: 3.114 ± 0.492
3.19GlyGln: 3.19 ± 0.644
4.937GlyArg: 4.937 ± 0.534
3.721GlySer: 3.721 ± 0.471
6.531GlyThr: 6.531 ± 1.038
6.076GlyVal: 6.076 ± 0.75
1.899GlyTrp: 1.899 ± 0.321
2.962GlyTyr: 2.962 ± 0.401
0.0GlyXaa: 0.0 ± 0.0
His
2.127HisAla: 2.127 ± 0.47
0.304HisCys: 0.304 ± 0.159
1.063HisAsp: 1.063 ± 0.29
0.835HisGlu: 0.835 ± 0.253
0.684HisPhe: 0.684 ± 0.251
1.747HisGly: 1.747 ± 0.394
0.38HisHis: 0.38 ± 0.172
0.911HisIle: 0.911 ± 0.24
0.911HisLys: 0.911 ± 0.284
2.127HisLeu: 2.127 ± 0.467
0.532HisMet: 0.532 ± 0.167
0.304HisAsn: 0.304 ± 0.147
1.291HisPro: 1.291 ± 0.378
0.456HisGln: 0.456 ± 0.165
1.215HisArg: 1.215 ± 0.338
1.063HisSer: 1.063 ± 0.281
0.532HisThr: 0.532 ± 0.182
1.519HisVal: 1.519 ± 0.316
0.076HisTrp: 0.076 ± 0.073
0.684HisTyr: 0.684 ± 0.194
0.0HisXaa: 0.0 ± 0.0
Ile
5.468IleAla: 5.468 ± 0.588
0.0IleCys: 0.0 ± 0.0
3.645IleAsp: 3.645 ± 0.492
3.114IleGlu: 3.114 ± 0.45
0.456IlePhe: 0.456 ± 0.178
2.886IleGly: 2.886 ± 0.476
1.139IleHis: 1.139 ± 0.393
2.278IleIle: 2.278 ± 0.378
2.354IleLys: 2.354 ± 0.559
4.329IleLeu: 4.329 ± 0.46
0.608IleMet: 0.608 ± 0.249
1.823IleAsn: 1.823 ± 0.446
2.354IlePro: 2.354 ± 0.489
0.987IleGln: 0.987 ± 0.235
3.266IleArg: 3.266 ± 0.531
1.975IleSer: 1.975 ± 0.349
4.253IleThr: 4.253 ± 0.699
3.645IleVal: 3.645 ± 0.624
0.684IleTrp: 0.684 ± 0.242
1.291IleTyr: 1.291 ± 0.277
0.0IleXaa: 0.0 ± 0.0
Lys
6.076LysAla: 6.076 ± 0.831
0.304LysCys: 0.304 ± 0.143
2.354LysAsp: 2.354 ± 0.531
2.202LysGlu: 2.202 ± 0.336
1.139LysPhe: 1.139 ± 0.304
2.734LysGly: 2.734 ± 0.403
0.456LysHis: 0.456 ± 0.175
1.975LysIle: 1.975 ± 0.464
2.127LysLys: 2.127 ± 0.484
3.342LysLeu: 3.342 ± 0.625
1.215LysMet: 1.215 ± 0.253
1.367LysAsn: 1.367 ± 0.316
3.418LysPro: 3.418 ± 0.587
1.063LysGln: 1.063 ± 0.358
3.19LysArg: 3.19 ± 0.511
2.506LysSer: 2.506 ± 0.507
2.81LysThr: 2.81 ± 0.427
2.734LysVal: 2.734 ± 0.372
0.38LysTrp: 0.38 ± 0.183
0.759LysTyr: 0.759 ± 0.229
0.0LysXaa: 0.0 ± 0.0
Leu
10.785LeuAla: 10.785 ± 1.003
0.456LeuCys: 0.456 ± 0.201
4.937LeuAsp: 4.937 ± 0.707
5.62LeuGlu: 5.62 ± 0.713
2.278LeuPhe: 2.278 ± 0.393
7.443LeuGly: 7.443 ± 0.687
0.911LeuHis: 0.911 ± 0.252
3.418LeuIle: 3.418 ± 0.535
3.949LeuLys: 3.949 ± 0.562
7.367LeuLeu: 7.367 ± 0.594
2.506LeuMet: 2.506 ± 0.365
3.342LeuAsn: 3.342 ± 0.547
4.557LeuPro: 4.557 ± 0.606
3.19LeuGln: 3.19 ± 0.515
5.772LeuArg: 5.772 ± 0.852
4.709LeuSer: 4.709 ± 0.604
6.0LeuThr: 6.0 ± 0.744
6.38LeuVal: 6.38 ± 0.675
0.987LeuTrp: 0.987 ± 0.238
1.747LeuTyr: 1.747 ± 0.357
0.0LeuXaa: 0.0 ± 0.0
Met
3.645MetAla: 3.645 ± 0.623
0.0MetCys: 0.0 ± 0.0
1.747MetAsp: 1.747 ± 0.363
0.835MetGlu: 0.835 ± 0.236
0.38MetPhe: 0.38 ± 0.17
1.367MetGly: 1.367 ± 0.257
0.076MetHis: 0.076 ± 0.064
0.835MetIle: 0.835 ± 0.275
1.367MetLys: 1.367 ± 0.385
1.443MetLeu: 1.443 ± 0.298
0.152MetMet: 0.152 ± 0.113
1.291MetAsn: 1.291 ± 0.276
1.291MetPro: 1.291 ± 0.339
0.228MetGln: 0.228 ± 0.124
1.291MetArg: 1.291 ± 0.313
0.911MetSer: 0.911 ± 0.243
2.354MetThr: 2.354 ± 0.376
1.671MetVal: 1.671 ± 0.319
0.608MetTrp: 0.608 ± 0.196
0.456MetTyr: 0.456 ± 0.172
0.0MetXaa: 0.0 ± 0.0
Asn
3.721AsnAla: 3.721 ± 0.525
0.076AsnCys: 0.076 ± 0.071
2.051AsnAsp: 2.051 ± 0.372
1.747AsnGlu: 1.747 ± 0.383
0.759AsnPhe: 0.759 ± 0.233
2.886AsnGly: 2.886 ± 0.437
0.608AsnHis: 0.608 ± 0.253
1.519AsnIle: 1.519 ± 0.344
1.291AsnLys: 1.291 ± 0.337
2.658AsnLeu: 2.658 ± 0.448
0.608AsnMet: 0.608 ± 0.194
1.823AsnAsn: 1.823 ± 0.416
2.582AsnPro: 2.582 ± 0.433
0.987AsnGln: 0.987 ± 0.234
1.975AsnArg: 1.975 ± 0.488
2.658AsnSer: 2.658 ± 0.547
2.127AsnThr: 2.127 ± 0.51
2.278AsnVal: 2.278 ± 0.401
0.608AsnTrp: 0.608 ± 0.252
0.987AsnTyr: 0.987 ± 0.25
0.0AsnXaa: 0.0 ± 0.0
Pro
8.278ProAla: 8.278 ± 0.803
0.456ProCys: 0.456 ± 0.179
3.342ProAsp: 3.342 ± 0.604
4.177ProGlu: 4.177 ± 0.747
1.671ProPhe: 1.671 ± 0.32
5.013ProGly: 5.013 ± 0.679
0.911ProHis: 0.911 ± 0.286
2.734ProIle: 2.734 ± 0.436
2.278ProLys: 2.278 ± 0.457
3.57ProLeu: 3.57 ± 0.54
0.532ProMet: 0.532 ± 0.197
1.595ProAsn: 1.595 ± 0.344
2.354ProPro: 2.354 ± 0.447
1.443ProGln: 1.443 ± 0.313
2.582ProArg: 2.582 ± 0.484
3.418ProSer: 3.418 ± 0.475
3.494ProThr: 3.494 ± 0.568
3.266ProVal: 3.266 ± 0.546
1.367ProTrp: 1.367 ± 0.383
0.608ProTyr: 0.608 ± 0.221
0.0ProXaa: 0.0 ± 0.0
Gln
4.709GlnAla: 4.709 ± 0.513
0.076GlnCys: 0.076 ± 0.083
1.595GlnAsp: 1.595 ± 0.357
2.202GlnGlu: 2.202 ± 0.379
1.139GlnPhe: 1.139 ± 0.32
2.506GlnGly: 2.506 ± 0.428
0.608GlnHis: 0.608 ± 0.201
1.671GlnIle: 1.671 ± 0.316
1.215GlnLys: 1.215 ± 0.235
4.861GlnLeu: 4.861 ± 0.502
0.532GlnMet: 0.532 ± 0.179
1.215GlnAsn: 1.215 ± 0.31
1.595GlnPro: 1.595 ± 0.386
0.532GlnGln: 0.532 ± 0.173
1.671GlnArg: 1.671 ± 0.307
1.367GlnSer: 1.367 ± 0.288
2.051GlnThr: 2.051 ± 0.372
1.975GlnVal: 1.975 ± 0.353
0.835GlnTrp: 0.835 ± 0.301
0.759GlnTyr: 0.759 ± 0.268
0.0GlnXaa: 0.0 ± 0.0
Arg
6.456ArgAla: 6.456 ± 0.726
0.304ArgCys: 0.304 ± 0.17
4.481ArgAsp: 4.481 ± 0.521
4.025ArgGlu: 4.025 ± 0.595
2.202ArgPhe: 2.202 ± 0.391
4.861ArgGly: 4.861 ± 0.649
1.519ArgHis: 1.519 ± 0.336
3.038ArgIle: 3.038 ± 0.455
2.354ArgLys: 2.354 ± 0.425
5.24ArgLeu: 5.24 ± 0.484
1.063ArgMet: 1.063 ± 0.289
1.823ArgAsn: 1.823 ± 0.393
3.342ArgPro: 3.342 ± 0.514
2.43ArgGln: 2.43 ± 0.355
5.62ArgArg: 5.62 ± 0.759
3.266ArgSer: 3.266 ± 0.535
3.949ArgThr: 3.949 ± 0.566
4.709ArgVal: 4.709 ± 0.686
1.595ArgTrp: 1.595 ± 0.337
1.367ArgTyr: 1.367 ± 0.325
0.0ArgXaa: 0.0 ± 0.0
Ser
5.164SerAla: 5.164 ± 0.737
0.076SerCys: 0.076 ± 0.069
3.494SerAsp: 3.494 ± 0.556
2.81SerGlu: 2.81 ± 0.346
2.43SerPhe: 2.43 ± 0.476
5.316SerGly: 5.316 ± 0.618
1.215SerHis: 1.215 ± 0.336
3.114SerIle: 3.114 ± 0.442
2.734SerLys: 2.734 ± 0.474
5.013SerLeu: 5.013 ± 0.579
1.443SerMet: 1.443 ± 0.43
1.671SerAsn: 1.671 ± 0.331
2.43SerPro: 2.43 ± 0.395
1.291SerGln: 1.291 ± 0.358
3.645SerArg: 3.645 ± 0.552
2.658SerSer: 2.658 ± 0.393
3.342SerThr: 3.342 ± 0.514
3.494SerVal: 3.494 ± 0.533
1.139SerTrp: 1.139 ± 0.264
0.835SerTyr: 0.835 ± 0.22
0.0SerXaa: 0.0 ± 0.0
Thr
8.506ThrAla: 8.506 ± 1.257
0.608ThrCys: 0.608 ± 0.243
4.177ThrAsp: 4.177 ± 0.62
4.025ThrGlu: 4.025 ± 0.494
3.038ThrPhe: 3.038 ± 0.414
5.544ThrGly: 5.544 ± 0.799
1.139ThrHis: 1.139 ± 0.324
3.57ThrIle: 3.57 ± 0.546
2.886ThrLys: 2.886 ± 0.551
5.468ThrLeu: 5.468 ± 0.782
0.911ThrMet: 0.911 ± 0.272
1.671ThrAsn: 1.671 ± 0.386
4.177ThrPro: 4.177 ± 0.616
1.215ThrGln: 1.215 ± 0.325
3.797ThrArg: 3.797 ± 0.554
4.101ThrSer: 4.101 ± 0.516
5.013ThrThr: 5.013 ± 0.939
4.709ThrVal: 4.709 ± 0.755
1.747ThrTrp: 1.747 ± 0.303
1.671ThrTyr: 1.671 ± 0.415
0.0ThrXaa: 0.0 ± 0.0
Val
8.81ValAla: 8.81 ± 0.832
0.304ValCys: 0.304 ± 0.15
5.088ValAsp: 5.088 ± 0.686
4.025ValGlu: 4.025 ± 0.512
2.506ValPhe: 2.506 ± 0.562
5.088ValGly: 5.088 ± 0.659
1.367ValHis: 1.367 ± 0.325
2.734ValIle: 2.734 ± 0.444
2.43ValLys: 2.43 ± 0.428
5.848ValLeu: 5.848 ± 0.729
1.519ValMet: 1.519 ± 0.291
2.354ValAsn: 2.354 ± 0.472
3.57ValPro: 3.57 ± 0.54
2.43ValGln: 2.43 ± 0.411
4.861ValArg: 4.861 ± 0.701
3.645ValSer: 3.645 ± 0.523
5.696ValThr: 5.696 ± 0.755
6.152ValVal: 6.152 ± 0.673
1.823ValTrp: 1.823 ± 0.664
2.43ValTyr: 2.43 ± 0.399
0.0ValXaa: 0.0 ± 0.0
Trp
2.051TrpAla: 2.051 ± 0.353
0.076TrpCys: 0.076 ± 0.077
0.759TrpAsp: 0.759 ± 0.213
1.443TrpGlu: 1.443 ± 0.338
0.759TrpPhe: 0.759 ± 0.208
1.367TrpGly: 1.367 ± 0.272
0.304TrpHis: 0.304 ± 0.165
1.063TrpIle: 1.063 ± 0.301
0.911TrpLys: 0.911 ± 0.273
1.671TrpLeu: 1.671 ± 0.3
0.911TrpMet: 0.911 ± 0.226
1.215TrpAsn: 1.215 ± 0.335
0.684TrpPro: 0.684 ± 0.253
1.139TrpGln: 1.139 ± 0.406
0.684TrpArg: 0.684 ± 0.269
1.747TrpSer: 1.747 ± 0.404
1.291TrpThr: 1.291 ± 0.332
2.278TrpVal: 2.278 ± 0.451
0.228TrpTrp: 0.228 ± 0.132
0.608TrpTyr: 0.608 ± 0.206
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.051TyrAla: 2.051 ± 0.383
0.228TyrCys: 0.228 ± 0.12
2.202TyrAsp: 2.202 ± 0.39
1.823TyrGlu: 1.823 ± 0.46
0.456TyrPhe: 0.456 ± 0.183
1.823TyrGly: 1.823 ± 0.338
0.684TyrHis: 0.684 ± 0.171
0.835TyrIle: 0.835 ± 0.19
0.456TyrLys: 0.456 ± 0.186
1.671TyrLeu: 1.671 ± 0.335
0.608TyrMet: 0.608 ± 0.195
0.987TyrAsn: 0.987 ± 0.266
1.215TyrPro: 1.215 ± 0.276
0.987TyrGln: 0.987 ± 0.368
2.278TyrArg: 2.278 ± 0.489
1.899TyrSer: 1.899 ± 0.402
2.127TyrThr: 2.127 ± 0.347
1.443TyrVal: 1.443 ± 0.404
0.684TyrTrp: 0.684 ± 0.225
0.152TyrTyr: 0.152 ± 0.16
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 68 proteins (13168 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski