Amino acid dipepetide frequency for Escherichia phage vB_EcoS-2004IV

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.639AlaAla: 8.639 ± 1.438
0.694AlaCys: 0.694 ± 0.222
4.243AlaAsp: 4.243 ± 0.621
5.708AlaGlu: 5.708 ± 0.681
3.625AlaPhe: 3.625 ± 0.561
5.862AlaGly: 5.862 ± 0.782
1.003AlaHis: 1.003 ± 0.307
6.634AlaIle: 6.634 ± 0.668
6.171AlaLys: 6.171 ± 1.122
7.791AlaLeu: 7.791 ± 1.094
2.006AlaMet: 2.006 ± 0.459
4.397AlaAsn: 4.397 ± 0.646
2.237AlaPro: 2.237 ± 0.377
3.471AlaGln: 3.471 ± 0.631
5.014AlaArg: 5.014 ± 0.677
5.94AlaSer: 5.94 ± 0.802
4.397AlaThr: 4.397 ± 0.63
4.937AlaVal: 4.937 ± 0.615
0.926AlaTrp: 0.926 ± 0.286
2.083AlaTyr: 2.083 ± 0.4
0.0AlaXaa: 0.0 ± 0.0
Cys
0.54CysAla: 0.54 ± 0.258
0.154CysCys: 0.154 ± 0.124
0.849CysAsp: 0.849 ± 0.3
0.926CysGlu: 0.926 ± 0.298
0.309CysPhe: 0.309 ± 0.158
1.388CysGly: 1.388 ± 0.354
0.231CysHis: 0.231 ± 0.13
0.309CysIle: 0.309 ± 0.192
1.234CysLys: 1.234 ± 0.307
1.003CysLeu: 1.003 ± 0.292
0.309CysMet: 0.309 ± 0.173
0.386CysAsn: 0.386 ± 0.232
0.386CysPro: 0.386 ± 0.179
0.077CysGln: 0.077 ± 0.076
0.771CysArg: 0.771 ± 0.277
1.08CysSer: 1.08 ± 0.364
1.003CysThr: 1.003 ± 0.346
0.849CysVal: 0.849 ± 0.274
0.309CysTrp: 0.309 ± 0.188
0.154CysTyr: 0.154 ± 0.101
0.0CysXaa: 0.0 ± 0.0
Asp
4.86AspAla: 4.86 ± 0.74
0.54AspCys: 0.54 ± 0.227
3.008AspAsp: 3.008 ± 0.576
4.782AspGlu: 4.782 ± 0.659
2.006AspPhe: 2.006 ± 0.356
6.788AspGly: 6.788 ± 0.759
0.849AspHis: 0.849 ± 0.385
4.165AspIle: 4.165 ± 0.476
4.551AspLys: 4.551 ± 0.56
4.32AspLeu: 4.32 ± 0.532
1.466AspMet: 1.466 ± 0.392
2.777AspAsn: 2.777 ± 0.511
1.697AspPro: 1.697 ± 0.346
1.311AspGln: 1.311 ± 0.312
2.083AspArg: 2.083 ± 0.463
2.777AspSer: 2.777 ± 0.479
3.548AspThr: 3.548 ± 0.573
3.78AspVal: 3.78 ± 0.491
1.234AspTrp: 1.234 ± 0.312
3.24AspTyr: 3.24 ± 0.471
0.0AspXaa: 0.0 ± 0.0
Glu
5.785GluAla: 5.785 ± 0.55
0.849GluCys: 0.849 ± 0.356
2.931GluAsp: 2.931 ± 0.511
4.088GluGlu: 4.088 ± 0.674
3.163GluPhe: 3.163 ± 0.493
3.934GluGly: 3.934 ± 0.545
0.386GluHis: 0.386 ± 0.191
5.708GluIle: 5.708 ± 0.585
3.471GluLys: 3.471 ± 0.749
6.171GluLeu: 6.171 ± 0.796
2.931GluMet: 2.931 ± 0.755
3.24GluAsn: 3.24 ± 0.584
1.928GluPro: 1.928 ± 0.479
2.931GluGln: 2.931 ± 0.625
2.931GluArg: 2.931 ± 0.546
4.86GluSer: 4.86 ± 0.575
3.394GluThr: 3.394 ± 0.511
4.937GluVal: 4.937 ± 0.582
0.463GluTrp: 0.463 ± 0.178
2.468GluTyr: 2.468 ± 0.396
0.0GluXaa: 0.0 ± 0.0
Phe
2.237PheAla: 2.237 ± 0.445
0.694PheCys: 0.694 ± 0.249
3.317PheAsp: 3.317 ± 0.483
2.546PheGlu: 2.546 ± 0.508
0.849PhePhe: 0.849 ± 0.28
3.471PheGly: 3.471 ± 0.529
0.771PheHis: 0.771 ± 0.277
2.391PheIle: 2.391 ± 0.428
2.623PheLys: 2.623 ± 0.602
1.928PheLeu: 1.928 ± 0.541
0.926PheMet: 0.926 ± 0.244
2.314PheAsn: 2.314 ± 0.341
1.08PhePro: 1.08 ± 0.284
2.006PheGln: 2.006 ± 0.412
1.774PheArg: 1.774 ± 0.413
2.546PheSer: 2.546 ± 0.495
2.468PheThr: 2.468 ± 0.439
2.468PheVal: 2.468 ± 0.453
0.386PheTrp: 0.386 ± 0.17
0.617PheTyr: 0.617 ± 0.186
0.0PheXaa: 0.0 ± 0.0
Gly
4.705GlyAla: 4.705 ± 0.739
1.466GlyCys: 1.466 ± 0.347
4.011GlyAsp: 4.011 ± 0.473
4.705GlyGlu: 4.705 ± 0.809
2.468GlyPhe: 2.468 ± 0.473
5.631GlyGly: 5.631 ± 1.025
0.849GlyHis: 0.849 ± 0.315
6.094GlyIle: 6.094 ± 0.637
5.708GlyLys: 5.708 ± 0.828
7.097GlyLeu: 7.097 ± 0.694
2.16GlyMet: 2.16 ± 0.418
3.163GlyAsn: 3.163 ± 0.421
0.771GlyPro: 0.771 ± 0.23
2.314GlyGln: 2.314 ± 0.48
2.931GlyArg: 2.931 ± 0.385
5.554GlySer: 5.554 ± 0.755
4.397GlyThr: 4.397 ± 0.804
6.325GlyVal: 6.325 ± 0.663
1.003GlyTrp: 1.003 ± 0.32
4.011GlyTyr: 4.011 ± 0.641
0.0GlyXaa: 0.0 ± 0.0
His
0.849HisAla: 0.849 ± 0.298
0.309HisCys: 0.309 ± 0.161
0.694HisAsp: 0.694 ± 0.218
0.771HisGlu: 0.771 ± 0.235
0.694HisPhe: 0.694 ± 0.26
0.771HisGly: 0.771 ± 0.324
0.386HisHis: 0.386 ± 0.2
1.311HisIle: 1.311 ± 0.373
1.003HisLys: 1.003 ± 0.282
1.08HisLeu: 1.08 ± 0.339
0.309HisMet: 0.309 ± 0.194
0.386HisAsn: 0.386 ± 0.186
0.231HisPro: 0.231 ± 0.181
0.386HisGln: 0.386 ± 0.167
0.771HisArg: 0.771 ± 0.261
0.54HisSer: 0.54 ± 0.23
0.771HisThr: 0.771 ± 0.249
0.694HisVal: 0.694 ± 0.264
0.077HisTrp: 0.077 ± 0.084
0.463HisTyr: 0.463 ± 0.185
0.0HisXaa: 0.0 ± 0.0
Ile
6.248IleAla: 6.248 ± 1.028
0.463IleCys: 0.463 ± 0.196
5.4IleAsp: 5.4 ± 0.62
3.78IleGlu: 3.78 ± 0.39
2.006IlePhe: 2.006 ± 0.315
4.474IleGly: 4.474 ± 0.608
1.08IleHis: 1.08 ± 0.299
3.703IleIle: 3.703 ± 0.611
5.091IleLys: 5.091 ± 0.682
3.471IleLeu: 3.471 ± 0.48
1.851IleMet: 1.851 ± 0.505
4.705IleAsn: 4.705 ± 0.712
3.008IlePro: 3.008 ± 0.523
2.546IleGln: 2.546 ± 0.614
3.934IleArg: 3.934 ± 0.468
5.94IleSer: 5.94 ± 0.824
5.245IleThr: 5.245 ± 0.632
3.471IleVal: 3.471 ± 0.494
0.849IleTrp: 0.849 ± 0.22
3.163IleTyr: 3.163 ± 0.606
0.0IleXaa: 0.0 ± 0.0
Lys
6.711LysAla: 6.711 ± 0.827
0.54LysCys: 0.54 ± 0.2
4.32LysAsp: 4.32 ± 0.61
5.4LysGlu: 5.4 ± 0.949
2.546LysPhe: 2.546 ± 0.435
2.854LysGly: 2.854 ± 0.487
0.694LysHis: 0.694 ± 0.23
3.78LysIle: 3.78 ± 0.496
3.394LysLys: 3.394 ± 0.578
4.782LysLeu: 4.782 ± 0.67
2.391LysMet: 2.391 ± 0.57
2.854LysAsn: 2.854 ± 0.598
2.16LysPro: 2.16 ± 0.418
2.854LysGln: 2.854 ± 0.424
2.777LysArg: 2.777 ± 0.621
4.551LysSer: 4.551 ± 0.759
3.163LysThr: 3.163 ± 0.426
4.86LysVal: 4.86 ± 0.596
0.771LysTrp: 0.771 ± 0.263
3.703LysTyr: 3.703 ± 0.504
0.0LysXaa: 0.0 ± 0.0
Leu
6.788LeuAla: 6.788 ± 0.792
0.849LeuCys: 0.849 ± 0.251
4.782LeuAsp: 4.782 ± 0.523
4.243LeuGlu: 4.243 ± 0.549
1.62LeuPhe: 1.62 ± 0.278
5.014LeuGly: 5.014 ± 0.765
1.08LeuHis: 1.08 ± 0.36
4.937LeuIle: 4.937 ± 0.625
3.934LeuLys: 3.934 ± 0.523
4.243LeuLeu: 4.243 ± 0.444
1.466LeuMet: 1.466 ± 0.329
3.471LeuAsn: 3.471 ± 0.501
2.7LeuPro: 2.7 ± 0.418
2.777LeuGln: 2.777 ± 0.907
3.857LeuArg: 3.857 ± 0.592
6.094LeuSer: 6.094 ± 0.667
4.782LeuThr: 4.782 ± 0.683
5.862LeuVal: 5.862 ± 0.467
0.309LeuTrp: 0.309 ± 0.171
2.16LeuTyr: 2.16 ± 0.449
0.0LeuXaa: 0.0 ± 0.0
Met
3.471MetAla: 3.471 ± 0.524
0.231MetCys: 0.231 ± 0.141
1.311MetAsp: 1.311 ± 0.397
0.926MetGlu: 0.926 ± 0.257
1.157MetPhe: 1.157 ± 0.364
0.771MetGly: 0.771 ± 0.234
0.231MetHis: 0.231 ± 0.165
2.006MetIle: 2.006 ± 0.349
1.543MetLys: 1.543 ± 0.354
2.006MetLeu: 2.006 ± 0.531
0.849MetMet: 0.849 ± 0.389
1.62MetAsn: 1.62 ± 0.493
0.617MetPro: 0.617 ± 0.198
1.08MetGln: 1.08 ± 0.273
1.466MetArg: 1.466 ± 0.354
1.774MetSer: 1.774 ± 0.394
2.083MetThr: 2.083 ± 0.391
1.388MetVal: 1.388 ± 0.322
0.309MetTrp: 0.309 ± 0.171
0.463MetTyr: 0.463 ± 0.209
0.0MetXaa: 0.0 ± 0.0
Asn
4.243AsnAla: 4.243 ± 0.585
0.54AsnCys: 0.54 ± 0.23
2.777AsnAsp: 2.777 ± 0.375
3.471AsnGlu: 3.471 ± 0.704
2.314AsnPhe: 2.314 ± 0.404
6.325AsnGly: 6.325 ± 1.089
0.617AsnHis: 0.617 ± 0.251
2.777AsnIle: 2.777 ± 0.371
2.931AsnLys: 2.931 ± 0.421
3.934AsnLeu: 3.934 ± 0.694
1.157AsnMet: 1.157 ± 0.272
2.854AsnAsn: 2.854 ± 0.414
1.851AsnPro: 1.851 ± 0.322
2.546AsnGln: 2.546 ± 0.64
2.237AsnArg: 2.237 ± 0.343
3.934AsnSer: 3.934 ± 0.486
2.006AsnThr: 2.006 ± 0.434
3.471AsnVal: 3.471 ± 0.456
0.54AsnTrp: 0.54 ± 0.179
1.388AsnTyr: 1.388 ± 0.377
0.0AsnXaa: 0.0 ± 0.0
Pro
2.854ProAla: 2.854 ± 0.375
0.309ProCys: 0.309 ± 0.213
2.16ProAsp: 2.16 ± 0.457
3.008ProGlu: 3.008 ± 0.467
1.466ProPhe: 1.466 ± 0.308
1.851ProGly: 1.851 ± 0.351
0.463ProHis: 0.463 ± 0.152
2.006ProIle: 2.006 ± 0.377
1.234ProLys: 1.234 ± 0.411
2.006ProLeu: 2.006 ± 0.358
0.54ProMet: 0.54 ± 0.207
1.234ProAsn: 1.234 ± 0.269
0.463ProPro: 0.463 ± 0.251
1.543ProGln: 1.543 ± 0.458
1.466ProArg: 1.466 ± 0.335
1.234ProSer: 1.234 ± 0.277
1.62ProThr: 1.62 ± 0.339
3.24ProVal: 3.24 ± 0.597
0.463ProTrp: 0.463 ± 0.233
1.466ProTyr: 1.466 ± 0.395
0.0ProXaa: 0.0 ± 0.0
Gln
4.474GlnAla: 4.474 ± 0.928
0.694GlnCys: 0.694 ± 0.273
1.774GlnAsp: 1.774 ± 0.403
2.854GlnGlu: 2.854 ± 0.532
1.62GlnPhe: 1.62 ± 0.301
2.623GlnGly: 2.623 ± 0.495
0.309GlnHis: 0.309 ± 0.147
3.471GlnIle: 3.471 ± 0.599
2.083GlnLys: 2.083 ± 0.449
2.777GlnLeu: 2.777 ± 0.646
0.926GlnMet: 0.926 ± 0.235
1.543GlnAsn: 1.543 ± 0.48
1.157GlnPro: 1.157 ± 0.402
2.391GlnGln: 2.391 ± 1.006
1.928GlnArg: 1.928 ± 0.519
2.777GlnSer: 2.777 ± 0.475
1.62GlnThr: 1.62 ± 0.344
2.468GlnVal: 2.468 ± 0.421
0.54GlnTrp: 0.54 ± 0.216
1.234GlnTyr: 1.234 ± 0.317
0.0GlnXaa: 0.0 ± 0.0
Arg
4.397ArgAla: 4.397 ± 0.636
0.771ArgCys: 0.771 ± 0.346
2.16ArgAsp: 2.16 ± 0.374
3.78ArgGlu: 3.78 ± 0.56
2.546ArgPhe: 2.546 ± 0.337
2.931ArgGly: 2.931 ± 0.463
0.463ArgHis: 0.463 ± 0.196
4.011ArgIle: 4.011 ± 0.512
4.86ArgLys: 4.86 ± 0.541
3.471ArgLeu: 3.471 ± 0.478
1.311ArgMet: 1.311 ± 0.349
2.006ArgAsn: 2.006 ± 0.434
1.466ArgPro: 1.466 ± 0.413
1.697ArgGln: 1.697 ± 0.411
2.854ArgArg: 2.854 ± 0.564
2.7ArgSer: 2.7 ± 0.378
1.851ArgThr: 1.851 ± 0.437
3.857ArgVal: 3.857 ± 0.554
0.463ArgTrp: 0.463 ± 0.186
2.16ArgTyr: 2.16 ± 0.443
0.0ArgXaa: 0.0 ± 0.0
Ser
5.4SerAla: 5.4 ± 0.811
0.694SerCys: 0.694 ± 0.318
5.168SerAsp: 5.168 ± 0.577
5.322SerGlu: 5.322 ± 0.62
2.468SerPhe: 2.468 ± 0.468
7.174SerGly: 7.174 ± 0.988
1.157SerHis: 1.157 ± 0.276
4.165SerIle: 4.165 ± 0.522
4.011SerLys: 4.011 ± 0.625
4.705SerLeu: 4.705 ± 0.678
1.543SerMet: 1.543 ± 0.352
4.165SerAsn: 4.165 ± 0.801
2.391SerPro: 2.391 ± 0.423
2.931SerGln: 2.931 ± 0.492
3.317SerArg: 3.317 ± 0.518
4.705SerSer: 4.705 ± 0.741
3.625SerThr: 3.625 ± 0.487
5.168SerVal: 5.168 ± 0.614
0.694SerTrp: 0.694 ± 0.267
2.314SerTyr: 2.314 ± 0.512
0.0SerXaa: 0.0 ± 0.0
Thr
5.168ThrAla: 5.168 ± 0.855
0.54ThrCys: 0.54 ± 0.211
2.854ThrAsp: 2.854 ± 0.326
2.623ThrGlu: 2.623 ± 0.384
2.391ThrPhe: 2.391 ± 0.413
6.402ThrGly: 6.402 ± 0.752
0.463ThrHis: 0.463 ± 0.184
4.397ThrIle: 4.397 ± 0.577
2.854ThrLys: 2.854 ± 0.626
3.24ThrLeu: 3.24 ± 0.395
0.849ThrMet: 0.849 ± 0.215
3.625ThrAsn: 3.625 ± 0.536
2.314ThrPro: 2.314 ± 0.365
2.391ThrGln: 2.391 ± 0.5
2.777ThrArg: 2.777 ± 0.437
3.548ThrSer: 3.548 ± 0.535
3.163ThrThr: 3.163 ± 0.573
3.857ThrVal: 3.857 ± 0.516
0.386ThrTrp: 0.386 ± 0.15
2.623ThrTyr: 2.623 ± 0.468
0.0ThrXaa: 0.0 ± 0.0
Val
5.4ValAla: 5.4 ± 0.776
1.157ValCys: 1.157 ± 0.34
4.86ValAsp: 4.86 ± 0.591
4.397ValGlu: 4.397 ± 0.773
2.546ValPhe: 2.546 ± 0.359
3.625ValGly: 3.625 ± 0.623
0.849ValHis: 0.849 ± 0.245
4.86ValIle: 4.86 ± 0.724
5.477ValLys: 5.477 ± 0.646
3.703ValLeu: 3.703 ± 0.494
1.311ValMet: 1.311 ± 0.414
4.165ValAsn: 4.165 ± 0.563
2.083ValPro: 2.083 ± 0.445
2.237ValGln: 2.237 ± 0.664
4.011ValArg: 4.011 ± 0.483
6.171ValSer: 6.171 ± 0.685
4.474ValThr: 4.474 ± 0.617
5.708ValVal: 5.708 ± 0.806
0.849ValTrp: 0.849 ± 0.237
2.468ValTyr: 2.468 ± 0.488
0.0ValXaa: 0.0 ± 0.0
Trp
0.54TrpAla: 0.54 ± 0.165
0.309TrpCys: 0.309 ± 0.16
0.694TrpAsp: 0.694 ± 0.187
0.771TrpGlu: 0.771 ± 0.282
0.617TrpPhe: 0.617 ± 0.287
0.771TrpGly: 0.771 ± 0.229
0.231TrpHis: 0.231 ± 0.114
1.003TrpIle: 1.003 ± 0.353
1.003TrpLys: 1.003 ± 0.271
0.617TrpLeu: 0.617 ± 0.182
0.231TrpMet: 0.231 ± 0.136
0.54TrpAsn: 0.54 ± 0.175
0.463TrpPro: 0.463 ± 0.234
0.463TrpGln: 0.463 ± 0.152
0.694TrpArg: 0.694 ± 0.228
0.771TrpSer: 0.771 ± 0.342
0.463TrpThr: 0.463 ± 0.174
0.54TrpVal: 0.54 ± 0.195
0.077TrpTrp: 0.077 ± 0.089
0.309TrpTyr: 0.309 ± 0.17
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.468TyrAla: 2.468 ± 0.434
0.463TyrCys: 0.463 ± 0.198
2.546TyrAsp: 2.546 ± 0.543
2.468TyrGlu: 2.468 ± 0.507
1.003TyrPhe: 1.003 ± 0.28
2.7TyrGly: 2.7 ± 0.371
0.386TyrHis: 0.386 ± 0.212
2.7TyrIle: 2.7 ± 0.438
1.928TyrLys: 1.928 ± 0.471
2.623TyrLeu: 2.623 ± 0.351
0.694TyrMet: 0.694 ± 0.231
2.468TyrAsn: 2.468 ± 0.389
1.62TyrPro: 1.62 ± 0.317
1.311TyrGln: 1.311 ± 0.363
2.16TyrArg: 2.16 ± 0.419
3.625TyrSer: 3.625 ± 0.698
2.391TyrThr: 2.391 ± 0.326
2.468TyrVal: 2.468 ± 0.499
0.463TyrTrp: 0.463 ± 0.198
1.466TyrTyr: 1.466 ± 0.38
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 66 proteins (12965 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski