Amino acid dipepetide frequency for Streptococcus phage phiJH1301-2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
3.399AlaAla: 3.399 ± 0.826
0.558AlaCys: 0.558 ± 0.163
3.348AlaAsp: 3.348 ± 0.559
4.616AlaGlu: 4.616 ± 0.558
3.145AlaPhe: 3.145 ± 0.428
3.906AlaGly: 3.906 ± 0.574
0.507AlaHis: 0.507 ± 0.201
5.53AlaIle: 5.53 ± 0.641
5.428AlaLys: 5.428 ± 0.576
4.718AlaLeu: 4.718 ± 0.583
1.725AlaMet: 1.725 ± 0.277
3.145AlaAsn: 3.145 ± 0.416
1.319AlaPro: 1.319 ± 0.313
2.384AlaGln: 2.384 ± 0.548
2.384AlaArg: 2.384 ± 0.324
4.363AlaSer: 4.363 ± 0.557
4.16AlaThr: 4.16 ± 0.451
3.551AlaVal: 3.551 ± 0.468
0.71AlaTrp: 0.71 ± 0.169
3.044AlaTyr: 3.044 ± 0.357
0.0AlaXaa: 0.0 ± 0.0
Cys
0.558CysAla: 0.558 ± 0.17
0.304CysCys: 0.304 ± 0.112
0.406CysAsp: 0.406 ± 0.134
0.609CysGlu: 0.609 ± 0.168
0.203CysPhe: 0.203 ± 0.098
0.659CysGly: 0.659 ± 0.155
0.304CysHis: 0.304 ± 0.132
0.609CysIle: 0.609 ± 0.153
0.659CysLys: 0.659 ± 0.277
0.812CysLeu: 0.812 ± 0.234
0.051CysMet: 0.051 ± 0.052
0.558CysAsn: 0.558 ± 0.162
0.406CysPro: 0.406 ± 0.161
0.761CysGln: 0.761 ± 0.185
0.558CysArg: 0.558 ± 0.175
0.558CysSer: 0.558 ± 0.189
0.254CysThr: 0.254 ± 0.121
0.507CysVal: 0.507 ± 0.175
0.0CysTrp: 0.0 ± 0.0
0.71CysTyr: 0.71 ± 0.212
0.0CysXaa: 0.0 ± 0.0
Asp
2.841AspAla: 2.841 ± 0.4
0.659AspCys: 0.659 ± 0.19
3.145AspAsp: 3.145 ± 0.432
5.276AspGlu: 5.276 ± 0.621
3.145AspPhe: 3.145 ± 0.341
4.819AspGly: 4.819 ± 0.527
0.71AspHis: 0.71 ± 0.168
4.515AspIle: 4.515 ± 0.484
3.805AspLys: 3.805 ± 0.445
5.175AspLeu: 5.175 ± 0.684
1.826AspMet: 1.826 ± 0.344
2.993AspAsn: 2.993 ± 0.438
1.623AspPro: 1.623 ± 0.307
1.37AspGln: 1.37 ± 0.223
2.435AspArg: 2.435 ± 0.509
3.196AspSer: 3.196 ± 0.474
2.892AspThr: 2.892 ± 0.333
2.689AspVal: 2.689 ± 0.375
0.964AspTrp: 0.964 ± 0.196
3.399AspTyr: 3.399 ± 0.476
0.0AspXaa: 0.0 ± 0.0
Glu
4.616GluAla: 4.616 ± 0.474
0.761GluCys: 0.761 ± 0.157
4.312GluAsp: 4.312 ± 0.524
6.646GluGlu: 6.646 ± 0.807
2.537GluPhe: 2.537 ± 0.44
4.515GluGly: 4.515 ± 0.397
1.471GluHis: 1.471 ± 0.311
4.819GluIle: 4.819 ± 0.517
7.052GluLys: 7.052 ± 0.849
8.726GluLeu: 8.726 ± 0.732
2.08GluMet: 2.08 ± 0.395
4.566GluAsn: 4.566 ± 0.615
1.471GluPro: 1.471 ± 0.287
3.805GluGln: 3.805 ± 0.362
2.739GluArg: 2.739 ± 0.342
4.109GluSer: 4.109 ± 0.472
4.667GluThr: 4.667 ± 0.464
4.718GluVal: 4.718 ± 0.38
0.812GluTrp: 0.812 ± 0.211
2.486GluTyr: 2.486 ± 0.535
0.0GluXaa: 0.0 ± 0.0
Phe
2.334PheAla: 2.334 ± 0.509
0.457PheCys: 0.457 ± 0.177
2.689PheAsp: 2.689 ± 0.363
3.45PheGlu: 3.45 ± 0.528
1.42PhePhe: 1.42 ± 0.318
2.739PheGly: 2.739 ± 0.348
0.761PheHis: 0.761 ± 0.187
2.689PheIle: 2.689 ± 0.432
3.196PheLys: 3.196 ± 0.487
3.348PheLeu: 3.348 ± 0.534
0.761PheMet: 0.761 ± 0.18
2.08PheAsn: 2.08 ± 0.321
0.913PhePro: 0.913 ± 0.261
1.471PheGln: 1.471 ± 0.259
1.877PheArg: 1.877 ± 0.284
3.044PheSer: 3.044 ± 0.428
2.334PheThr: 2.334 ± 0.397
2.537PheVal: 2.537 ± 0.394
0.558PheTrp: 0.558 ± 0.185
2.587PheTyr: 2.587 ± 0.285
0.0PheXaa: 0.0 ± 0.0
Gly
2.892GlyAla: 2.892 ± 0.54
0.558GlyCys: 0.558 ± 0.141
3.145GlyAsp: 3.145 ± 0.522
3.551GlyGlu: 3.551 ± 0.429
3.602GlyPhe: 3.602 ± 0.44
3.5GlyGly: 3.5 ± 0.544
1.471GlyHis: 1.471 ± 0.292
5.682GlyIle: 5.682 ± 0.694
4.972GlyLys: 4.972 ± 0.475
5.428GlyLeu: 5.428 ± 0.574
1.978GlyMet: 1.978 ± 0.375
3.551GlyAsn: 3.551 ± 0.423
0.558GlyPro: 0.558 ± 0.165
2.638GlyGln: 2.638 ± 0.451
3.044GlyArg: 3.044 ± 0.362
3.348GlySer: 3.348 ± 0.37
4.058GlyThr: 4.058 ± 0.418
3.805GlyVal: 3.805 ± 0.457
0.761GlyTrp: 0.761 ± 0.186
3.551GlyTyr: 3.551 ± 0.445
0.0GlyXaa: 0.0 ± 0.0
His
0.761HisAla: 0.761 ± 0.177
0.254HisCys: 0.254 ± 0.109
0.964HisAsp: 0.964 ± 0.219
1.015HisGlu: 1.015 ± 0.254
0.964HisPhe: 0.964 ± 0.264
1.471HisGly: 1.471 ± 0.261
0.862HisHis: 0.862 ± 0.222
1.218HisIle: 1.218 ± 0.222
1.319HisLys: 1.319 ± 0.266
1.674HisLeu: 1.674 ± 0.287
0.304HisMet: 0.304 ± 0.13
0.862HisAsn: 0.862 ± 0.201
1.065HisPro: 1.065 ± 0.244
0.761HisGln: 0.761 ± 0.267
1.116HisArg: 1.116 ± 0.244
0.862HisSer: 0.862 ± 0.233
1.065HisThr: 1.065 ± 0.221
0.964HisVal: 0.964 ± 0.24
0.203HisTrp: 0.203 ± 0.092
1.116HisTyr: 1.116 ± 0.255
0.0HisXaa: 0.0 ± 0.0
Ile
4.616IleAla: 4.616 ± 0.511
0.558IleCys: 0.558 ± 0.167
5.631IleAsp: 5.631 ± 0.495
4.616IleGlu: 4.616 ± 0.66
2.486IlePhe: 2.486 ± 0.403
4.769IleGly: 4.769 ± 0.572
1.116IleHis: 1.116 ± 0.229
3.957IleIle: 3.957 ± 0.48
5.276IleLys: 5.276 ± 0.518
5.377IleLeu: 5.377 ± 0.463
1.471IleMet: 1.471 ± 0.286
3.754IleAsn: 3.754 ± 0.468
2.283IlePro: 2.283 ± 0.281
2.283IleGln: 2.283 ± 0.284
2.942IleArg: 2.942 ± 0.393
5.175IleSer: 5.175 ± 0.744
4.414IleThr: 4.414 ± 0.595
5.175IleVal: 5.175 ± 0.619
1.065IleTrp: 1.065 ± 0.264
2.435IleTyr: 2.435 ± 0.401
0.0IleXaa: 0.0 ± 0.0
Lys
5.631LysAla: 5.631 ± 0.515
0.457LysCys: 0.457 ± 0.149
3.5LysAsp: 3.5 ± 0.487
6.696LysGlu: 6.696 ± 0.673
2.486LysPhe: 2.486 ± 0.377
4.718LysGly: 4.718 ± 0.613
1.268LysHis: 1.268 ± 0.283
5.124LysIle: 5.124 ± 0.403
6.189LysLys: 6.189 ± 0.87
6.443LysLeu: 6.443 ± 0.583
2.334LysMet: 2.334 ± 0.376
3.653LysAsn: 3.653 ± 0.459
2.08LysPro: 2.08 ± 0.29
3.754LysGln: 3.754 ± 0.486
3.805LysArg: 3.805 ± 0.553
4.109LysSer: 4.109 ± 0.526
4.414LysThr: 4.414 ± 0.477
5.53LysVal: 5.53 ± 0.563
1.268LysTrp: 1.268 ± 0.254
3.095LysTyr: 3.095 ± 0.463
0.0LysXaa: 0.0 ± 0.0
Leu
6.392LeuAla: 6.392 ± 0.776
0.558LeuCys: 0.558 ± 0.159
5.124LeuAsp: 5.124 ± 0.443
7.559LeuGlu: 7.559 ± 0.653
3.297LeuPhe: 3.297 ± 0.441
4.87LeuGly: 4.87 ± 0.507
1.623LeuHis: 1.623 ± 0.297
5.175LeuIle: 5.175 ± 0.459
7.052LeuLys: 7.052 ± 0.49
8.776LeuLeu: 8.776 ± 0.984
2.587LeuMet: 2.587 ± 0.349
4.16LeuAsn: 4.16 ± 0.427
3.196LeuPro: 3.196 ± 0.392
3.196LeuGln: 3.196 ± 0.436
4.008LeuArg: 4.008 ± 0.415
7.407LeuSer: 7.407 ± 0.617
6.088LeuThr: 6.088 ± 0.562
6.189LeuVal: 6.189 ± 0.652
0.761LeuTrp: 0.761 ± 0.174
4.16LeuTyr: 4.16 ± 0.624
0.0LeuXaa: 0.0 ± 0.0
Met
1.623MetAla: 1.623 ± 0.296
0.203MetCys: 0.203 ± 0.11
1.826MetAsp: 1.826 ± 0.35
1.978MetGlu: 1.978 ± 0.343
1.065MetPhe: 1.065 ± 0.265
1.167MetGly: 1.167 ± 0.311
0.152MetHis: 0.152 ± 0.097
1.573MetIle: 1.573 ± 0.267
1.776MetLys: 1.776 ± 0.279
1.826MetLeu: 1.826 ± 0.329
1.065MetMet: 1.065 ± 0.309
0.964MetAsn: 0.964 ± 0.225
0.558MetPro: 0.558 ± 0.17
0.812MetGln: 0.812 ± 0.206
1.116MetArg: 1.116 ± 0.205
1.725MetSer: 1.725 ± 0.333
1.978MetThr: 1.978 ± 0.335
1.37MetVal: 1.37 ± 0.254
0.152MetTrp: 0.152 ± 0.087
0.71MetTyr: 0.71 ± 0.185
0.0MetXaa: 0.0 ± 0.0
Asn
3.805AsnAla: 3.805 ± 0.605
0.355AsnCys: 0.355 ± 0.142
2.587AsnAsp: 2.587 ± 0.323
3.196AsnGlu: 3.196 ± 0.433
2.486AsnPhe: 2.486 ± 0.393
4.414AsnGly: 4.414 ± 0.455
1.37AsnHis: 1.37 ± 0.223
3.348AsnIle: 3.348 ± 0.403
3.399AsnLys: 3.399 ± 0.472
5.022AsnLeu: 5.022 ± 0.639
0.761AsnMet: 0.761 ± 0.206
2.942AsnAsn: 2.942 ± 0.543
2.283AsnPro: 2.283 ± 0.356
2.587AsnGln: 2.587 ± 0.407
2.435AsnArg: 2.435 ± 0.388
3.044AsnSer: 3.044 ± 0.404
1.877AsnThr: 1.877 ± 0.37
2.537AsnVal: 2.537 ± 0.421
0.761AsnTrp: 0.761 ± 0.228
1.826AsnTyr: 1.826 ± 0.29
0.0AsnXaa: 0.0 ± 0.0
Pro
1.319ProAla: 1.319 ± 0.271
0.304ProCys: 0.304 ± 0.12
1.776ProAsp: 1.776 ± 0.326
2.689ProGlu: 2.689 ± 0.413
1.065ProPhe: 1.065 ± 0.222
0.862ProGly: 0.862 ± 0.217
0.659ProHis: 0.659 ± 0.151
1.826ProIle: 1.826 ± 0.331
2.638ProLys: 2.638 ± 0.413
2.689ProLeu: 2.689 ± 0.366
0.406ProMet: 0.406 ± 0.129
1.623ProAsn: 1.623 ± 0.226
1.015ProPro: 1.015 ± 0.248
0.913ProGln: 0.913 ± 0.233
1.522ProArg: 1.522 ± 0.279
2.283ProSer: 2.283 ± 0.324
1.978ProThr: 1.978 ± 0.323
1.877ProVal: 1.877 ± 0.293
0.355ProTrp: 0.355 ± 0.124
1.319ProTyr: 1.319 ± 0.267
0.0ProXaa: 0.0 ± 0.0
Gln
3.805GlnAla: 3.805 ± 0.525
0.457GlnCys: 0.457 ± 0.158
2.029GlnAsp: 2.029 ± 0.292
3.5GlnGlu: 3.5 ± 0.511
1.725GlnPhe: 1.725 ± 0.264
1.928GlnGly: 1.928 ± 0.29
0.457GlnHis: 0.457 ± 0.115
2.79GlnIle: 2.79 ± 0.37
2.587GlnLys: 2.587 ± 0.363
4.008GlnLeu: 4.008 ± 0.528
1.116GlnMet: 1.116 ± 0.256
1.978GlnAsn: 1.978 ± 0.31
1.218GlnPro: 1.218 ± 0.266
1.623GlnGln: 1.623 ± 0.324
1.37GlnArg: 1.37 ± 0.342
2.131GlnSer: 2.131 ± 0.404
3.044GlnThr: 3.044 ± 0.601
3.348GlnVal: 3.348 ± 0.464
0.71GlnTrp: 0.71 ± 0.221
0.812GlnTyr: 0.812 ± 0.228
0.0GlnXaa: 0.0 ± 0.0
Arg
2.232ArgAla: 2.232 ± 0.296
0.507ArgCys: 0.507 ± 0.161
2.537ArgAsp: 2.537 ± 0.427
3.145ArgGlu: 3.145 ± 0.384
1.725ArgPhe: 1.725 ± 0.364
2.689ArgGly: 2.689 ± 0.435
0.71ArgHis: 0.71 ± 0.205
2.942ArgIle: 2.942 ± 0.379
4.008ArgLys: 4.008 ± 0.564
4.769ArgLeu: 4.769 ± 0.512
0.609ArgMet: 0.609 ± 0.159
2.232ArgAsn: 2.232 ± 0.34
1.471ArgPro: 1.471 ± 0.287
2.232ArgGln: 2.232 ± 0.332
1.928ArgArg: 1.928 ± 0.398
2.384ArgSer: 2.384 ± 0.287
2.79ArgThr: 2.79 ± 0.622
2.993ArgVal: 2.993 ± 0.398
0.913ArgTrp: 0.913 ± 0.221
1.471ArgTyr: 1.471 ± 0.291
0.0ArgXaa: 0.0 ± 0.0
Ser
4.058SerAla: 4.058 ± 0.57
0.609SerCys: 0.609 ± 0.172
3.754SerAsp: 3.754 ± 0.466
3.805SerGlu: 3.805 ± 0.423
3.145SerPhe: 3.145 ± 0.433
4.312SerGly: 4.312 ± 0.487
1.522SerHis: 1.522 ± 0.248
5.022SerIle: 5.022 ± 0.581
4.718SerLys: 4.718 ± 0.49
5.124SerLeu: 5.124 ± 0.507
1.065SerMet: 1.065 ± 0.176
2.739SerAsn: 2.739 ± 0.4
1.978SerPro: 1.978 ± 0.299
2.587SerGln: 2.587 ± 0.521
2.739SerArg: 2.739 ± 0.359
5.327SerSer: 5.327 ± 0.744
3.957SerThr: 3.957 ± 0.413
4.16SerVal: 4.16 ± 0.439
1.42SerTrp: 1.42 ± 0.23
2.384SerTyr: 2.384 ± 0.317
0.0SerXaa: 0.0 ± 0.0
Thr
4.16ThrAla: 4.16 ± 0.402
0.152ThrCys: 0.152 ± 0.083
3.348ThrAsp: 3.348 ± 0.368
5.327ThrGlu: 5.327 ± 0.582
2.435ThrPhe: 2.435 ± 0.432
3.653ThrGly: 3.653 ± 0.534
1.319ThrHis: 1.319 ± 0.25
4.667ThrIle: 4.667 ± 0.604
4.261ThrLys: 4.261 ± 0.488
5.53ThrLeu: 5.53 ± 0.482
1.319ThrMet: 1.319 ± 0.25
3.145ThrAsn: 3.145 ± 0.43
1.978ThrPro: 1.978 ± 0.389
2.131ThrGln: 2.131 ± 0.607
2.181ThrArg: 2.181 ± 0.402
4.008ThrSer: 4.008 ± 0.673
4.464ThrThr: 4.464 ± 0.711
4.819ThrVal: 4.819 ± 0.566
0.761ThrTrp: 0.761 ± 0.18
2.435ThrTyr: 2.435 ± 0.392
0.0ThrXaa: 0.0 ± 0.0
Val
4.211ValAla: 4.211 ± 0.512
0.71ValCys: 0.71 ± 0.205
3.703ValAsp: 3.703 ± 0.51
4.87ValGlu: 4.87 ± 0.629
2.384ValPhe: 2.384 ± 0.398
3.399ValGly: 3.399 ± 0.523
1.015ValHis: 1.015 ± 0.234
4.008ValIle: 4.008 ± 0.408
4.566ValLys: 4.566 ± 0.417
6.95ValLeu: 6.95 ± 0.51
1.218ValMet: 1.218 ± 0.246
2.638ValAsn: 2.638 ± 0.348
2.131ValPro: 2.131 ± 0.301
2.334ValGln: 2.334 ± 0.353
3.247ValArg: 3.247 ± 0.557
3.906ValSer: 3.906 ± 0.654
4.515ValThr: 4.515 ± 0.455
3.196ValVal: 3.196 ± 0.453
0.964ValTrp: 0.964 ± 0.226
2.739ValTyr: 2.739 ± 0.427
0.0ValXaa: 0.0 ± 0.0
Trp
0.659TrpAla: 0.659 ± 0.196
0.254TrpCys: 0.254 ± 0.118
0.304TrpAsp: 0.304 ± 0.114
1.471TrpGlu: 1.471 ± 0.265
0.609TrpPhe: 0.609 ± 0.213
0.609TrpGly: 0.609 ± 0.143
0.355TrpHis: 0.355 ± 0.153
1.065TrpIle: 1.065 ± 0.289
1.015TrpLys: 1.015 ± 0.253
1.42TrpLeu: 1.42 ± 0.204
0.304TrpMet: 0.304 ± 0.109
1.167TrpAsn: 1.167 ± 0.252
0.101TrpPro: 0.101 ± 0.068
0.761TrpGln: 0.761 ± 0.212
0.507TrpArg: 0.507 ± 0.188
0.862TrpSer: 0.862 ± 0.284
1.218TrpThr: 1.218 ± 0.306
0.659TrpVal: 0.659 ± 0.187
0.254TrpTrp: 0.254 ± 0.112
0.304TrpTyr: 0.304 ± 0.114
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.029TyrAla: 2.029 ± 0.275
0.812TyrCys: 0.812 ± 0.198
3.348TyrAsp: 3.348 ± 0.463
2.942TyrGlu: 2.942 ± 0.446
1.319TyrPhe: 1.319 ± 0.313
3.095TyrGly: 3.095 ± 0.478
1.167TyrHis: 1.167 ± 0.245
2.841TyrIle: 2.841 ± 0.476
2.638TyrLys: 2.638 ± 0.367
4.363TyrLeu: 4.363 ± 0.486
0.609TyrMet: 0.609 ± 0.208
2.283TyrAsn: 2.283 ± 0.364
1.471TyrPro: 1.471 ± 0.236
2.232TyrGln: 2.232 ± 0.291
2.334TyrArg: 2.334 ± 0.466
2.587TyrSer: 2.587 ± 0.36
1.928TyrThr: 1.928 ± 0.315
2.08TyrVal: 2.08 ± 0.333
0.507TyrTrp: 0.507 ± 0.148
1.471TyrTyr: 1.471 ± 0.318
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 76 proteins (19713 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski