Amycolatopsis acidicola

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Amycolatopsis

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8848 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5N0V1S0|A0A5N0V1S0_9PSEU Helix-turn-helix transcriptional regulator OS=Amycolatopsis acidicola OX=2596893 GN=FPZ12_023710 PE=4 SV=1
MM1 pKa = 8.06SEE3 pKa = 3.93EE4 pKa = 3.9PRR6 pKa = 11.84YY7 pKa = 10.36LGLSPYY13 pKa = 10.48LYY15 pKa = 9.74YY16 pKa = 10.64TDD18 pKa = 3.64ATEE21 pKa = 4.2ALDD24 pKa = 3.17WLVRR28 pKa = 11.84VFGFTEE34 pKa = 4.08RR35 pKa = 11.84VRR37 pKa = 11.84YY38 pKa = 9.67VDD40 pKa = 3.11GSGVVFQATLSAGDD54 pKa = 3.81ADD56 pKa = 3.62IVLASVGEE64 pKa = 4.63DD65 pKa = 3.13YY66 pKa = 10.78WEE68 pKa = 4.29SKK70 pKa = 10.76GVDD73 pKa = 3.16RR74 pKa = 11.84PVGQLNVIFVPDD86 pKa = 3.56ADD88 pKa = 3.66AQYY91 pKa = 11.4EE92 pKa = 4.34LVCEE96 pKa = 4.24ALGEE100 pKa = 4.62GCDD103 pKa = 3.62VAPPQDD109 pKa = 3.48QPYY112 pKa = 8.0GARR115 pKa = 11.84VFTVADD121 pKa = 3.03IGGNSWTFWQQISDD135 pKa = 3.98TADD138 pKa = 3.5LPSGWQEE145 pKa = 3.68IRR147 pKa = 11.84PEE149 pKa = 3.79

Molecular weight: 16.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5N0VFU2|A0A5N0VFU2_9PSEU Dihydroorotate dehydrogenase OS=Amycolatopsis acidicola OX=2596893 GN=FPZ12_008655 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.72HH17 pKa = 5.63RR18 pKa = 11.84KK19 pKa = 7.68LLRR22 pKa = 11.84RR23 pKa = 11.84TRR25 pKa = 11.84MQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 9.69QGKK33 pKa = 8.53

Molecular weight: 4.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8848

0

8848

2858604

33

29662

323.1

34.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.076 ± 0.032

0.764 ± 0.01

5.719 ± 0.021

6.031 ± 0.031

3.05 ± 0.014

9.385 ± 0.025

2.162 ± 0.012

3.645 ± 0.019

2.236 ± 0.018

10.539 ± 0.029

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.739 ± 0.012

1.973 ± 0.012

5.725 ± 0.02

2.825 ± 0.017

7.731 ± 0.031

5.241 ± 0.02

5.844 ± 0.02

8.791 ± 0.025

1.474 ± 0.01

2.049 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski