Amino acid dipepetide frequency for Mycobacterium phage Watermelon

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.762AlaAla: 12.762 ± 1.145
0.692AlaCys: 0.692 ± 0.188
6.538AlaAsp: 6.538 ± 0.571
6.098AlaGlu: 6.098 ± 0.674
3.269AlaPhe: 3.269 ± 0.51
7.292AlaGly: 7.292 ± 0.844
1.383AlaHis: 1.383 ± 0.334
4.086AlaIle: 4.086 ± 0.487
4.275AlaLys: 4.275 ± 0.681
9.241AlaLeu: 9.241 ± 0.919
2.326AlaMet: 2.326 ± 0.451
2.515AlaAsn: 2.515 ± 0.427
5.155AlaPro: 5.155 ± 0.698
2.829AlaGln: 2.829 ± 0.43
5.658AlaArg: 5.658 ± 0.573
5.218AlaSer: 5.218 ± 0.499
5.595AlaThr: 5.595 ± 0.565
8.613AlaVal: 8.613 ± 0.709
1.823AlaTrp: 1.823 ± 0.4
2.892AlaTyr: 2.892 ± 0.419
0.0AlaXaa: 0.0 ± 0.0
Cys
0.629CysAla: 0.629 ± 0.233
0.0CysCys: 0.0 ± 0.0
0.377CysAsp: 0.377 ± 0.153
0.754CysGlu: 0.754 ± 0.218
0.251CysPhe: 0.251 ± 0.131
0.629CysGly: 0.629 ± 0.242
0.126CysHis: 0.126 ± 0.079
0.251CysIle: 0.251 ± 0.145
0.251CysLys: 0.251 ± 0.131
0.377CysLeu: 0.377 ± 0.173
0.126CysMet: 0.126 ± 0.081
0.189CysAsn: 0.189 ± 0.105
0.189CysPro: 0.189 ± 0.099
0.126CysGln: 0.126 ± 0.09
0.314CysArg: 0.314 ± 0.128
0.314CysSer: 0.314 ± 0.15
0.314CysThr: 0.314 ± 0.148
0.629CysVal: 0.629 ± 0.232
0.251CysTrp: 0.251 ± 0.118
0.126CysTyr: 0.126 ± 0.1
0.0CysXaa: 0.0 ± 0.0
Asp
6.098AspAla: 6.098 ± 0.615
0.629AspCys: 0.629 ± 0.195
4.463AspAsp: 4.463 ± 0.492
3.458AspGlu: 3.458 ± 0.489
2.515AspPhe: 2.515 ± 0.403
5.909AspGly: 5.909 ± 0.679
1.069AspHis: 1.069 ± 0.281
2.892AspIle: 2.892 ± 0.459
2.955AspLys: 2.955 ± 0.528
6.978AspLeu: 6.978 ± 0.727
1.509AspMet: 1.509 ± 0.248
1.76AspAsn: 1.76 ± 0.337
4.652AspPro: 4.652 ± 0.518
1.509AspGln: 1.509 ± 0.306
3.709AspArg: 3.709 ± 0.392
3.458AspSer: 3.458 ± 0.494
4.086AspThr: 4.086 ± 0.534
4.526AspVal: 4.526 ± 0.565
1.635AspTrp: 1.635 ± 0.318
2.137AspTyr: 2.137 ± 0.369
0.0AspXaa: 0.0 ± 0.0
Glu
6.789GluAla: 6.789 ± 0.706
0.377GluCys: 0.377 ± 0.167
4.715GluAsp: 4.715 ± 0.615
4.652GluGlu: 4.652 ± 0.589
2.012GluPhe: 2.012 ± 0.39
4.463GluGly: 4.463 ± 0.534
1.32GluHis: 1.32 ± 0.275
3.269GluIle: 3.269 ± 0.467
2.955GluLys: 2.955 ± 0.485
6.727GluLeu: 6.727 ± 0.568
1.572GluMet: 1.572 ± 0.321
1.823GluAsn: 1.823 ± 0.351
2.766GluPro: 2.766 ± 0.43
2.64GluGln: 2.64 ± 0.414
3.583GluArg: 3.583 ± 0.553
3.646GluSer: 3.646 ± 0.479
4.023GluThr: 4.023 ± 0.64
5.029GluVal: 5.029 ± 0.578
1.509GluTrp: 1.509 ± 0.336
2.452GluTyr: 2.452 ± 0.577
0.0GluXaa: 0.0 ± 0.0
Phe
2.64PheAla: 2.64 ± 0.406
0.251PheCys: 0.251 ± 0.144
2.829PheAsp: 2.829 ± 0.345
1.886PheGlu: 1.886 ± 0.355
0.629PhePhe: 0.629 ± 0.204
3.646PheGly: 3.646 ± 0.554
0.692PheHis: 0.692 ± 0.225
1.194PheIle: 1.194 ± 0.295
1.383PheLys: 1.383 ± 0.281
2.64PheLeu: 2.64 ± 0.442
0.566PheMet: 0.566 ± 0.211
1.32PheAsn: 1.32 ± 0.294
1.509PhePro: 1.509 ± 0.304
0.943PheGln: 0.943 ± 0.229
1.886PheArg: 1.886 ± 0.345
2.137PheSer: 2.137 ± 0.393
2.2PheThr: 2.2 ± 0.337
1.697PheVal: 1.697 ± 0.341
0.692PheTrp: 0.692 ± 0.191
1.006PheTyr: 1.006 ± 0.314
0.0PheXaa: 0.0 ± 0.0
Gly
7.355GlyAla: 7.355 ± 0.968
0.566GlyCys: 0.566 ± 0.199
5.721GlyAsp: 5.721 ± 0.521
4.589GlyGlu: 4.589 ± 0.542
2.64GlyPhe: 2.64 ± 0.504
9.241GlyGly: 9.241 ± 2.256
1.697GlyHis: 1.697 ± 0.342
4.652GlyIle: 4.652 ± 0.782
3.395GlyLys: 3.395 ± 0.461
7.732GlyLeu: 7.732 ± 0.826
1.886GlyMet: 1.886 ± 0.395
3.018GlyAsn: 3.018 ± 0.41
3.898GlyPro: 3.898 ± 0.583
2.389GlyGln: 2.389 ± 0.372
4.526GlyArg: 4.526 ± 0.505
6.601GlySer: 6.601 ± 0.878
5.029GlyThr: 5.029 ± 0.711
5.721GlyVal: 5.721 ± 0.641
2.577GlyTrp: 2.577 ± 0.389
2.703GlyTyr: 2.703 ± 0.404
0.0GlyXaa: 0.0 ± 0.0
His
1.697HisAla: 1.697 ± 0.348
0.126HisCys: 0.126 ± 0.128
1.069HisAsp: 1.069 ± 0.25
1.446HisGlu: 1.446 ± 0.311
0.754HisPhe: 0.754 ± 0.178
1.383HisGly: 1.383 ± 0.323
0.692HisHis: 0.692 ± 0.23
0.943HisIle: 0.943 ± 0.238
1.069HisLys: 1.069 ± 0.281
1.697HisLeu: 1.697 ± 0.351
0.189HisMet: 0.189 ± 0.108
0.126HisAsn: 0.126 ± 0.084
1.383HisPro: 1.383 ± 0.301
0.88HisGln: 0.88 ± 0.219
1.383HisArg: 1.383 ± 0.332
0.754HisSer: 0.754 ± 0.179
1.006HisThr: 1.006 ± 0.271
1.823HisVal: 1.823 ± 0.358
0.566HisTrp: 0.566 ± 0.17
0.692HisTyr: 0.692 ± 0.243
0.0HisXaa: 0.0 ± 0.0
Ile
6.098IleAla: 6.098 ± 0.706
0.189IleCys: 0.189 ± 0.106
3.52IleAsp: 3.52 ± 0.413
3.583IleGlu: 3.583 ± 0.559
0.754IlePhe: 0.754 ± 0.199
4.338IleGly: 4.338 ± 0.548
0.88IleHis: 0.88 ± 0.237
2.2IleIle: 2.2 ± 0.429
1.697IleLys: 1.697 ± 0.332
3.52IleLeu: 3.52 ± 0.47
0.817IleMet: 0.817 ± 0.21
1.697IleAsn: 1.697 ± 0.385
3.143IlePro: 3.143 ± 0.395
1.697IleGln: 1.697 ± 0.361
3.269IleArg: 3.269 ± 0.438
3.332IleSer: 3.332 ± 0.471
3.772IleThr: 3.772 ± 0.432
2.515IleVal: 2.515 ± 0.537
0.692IleTrp: 0.692 ± 0.218
1.823IleTyr: 1.823 ± 0.317
0.0IleXaa: 0.0 ± 0.0
Lys
3.835LysAla: 3.835 ± 0.594
0.251LysCys: 0.251 ± 0.126
2.766LysAsp: 2.766 ± 0.442
2.263LysGlu: 2.263 ± 0.374
1.509LysPhe: 1.509 ± 0.256
2.703LysGly: 2.703 ± 0.361
1.257LysHis: 1.257 ± 0.314
2.389LysIle: 2.389 ± 0.429
2.389LysLys: 2.389 ± 0.513
3.08LysLeu: 3.08 ± 0.426
1.257LysMet: 1.257 ± 0.238
1.886LysAsn: 1.886 ± 0.291
2.577LysPro: 2.577 ± 0.427
1.76LysGln: 1.76 ± 0.388
3.143LysArg: 3.143 ± 0.55
2.703LysSer: 2.703 ± 0.378
2.452LysThr: 2.452 ± 0.401
3.709LysVal: 3.709 ± 0.515
0.692LysTrp: 0.692 ± 0.228
1.006LysTyr: 1.006 ± 0.272
0.0LysXaa: 0.0 ± 0.0
Leu
8.864LeuAla: 8.864 ± 0.813
0.314LeuCys: 0.314 ± 0.136
6.412LeuAsp: 6.412 ± 0.554
5.846LeuGlu: 5.846 ± 0.677
2.389LeuPhe: 2.389 ± 0.391
7.607LeuGly: 7.607 ± 0.742
1.76LeuHis: 1.76 ± 0.369
4.401LeuIle: 4.401 ± 0.506
4.086LeuLys: 4.086 ± 0.523
6.161LeuLeu: 6.161 ± 0.625
1.509LeuMet: 1.509 ± 0.272
2.955LeuAsn: 2.955 ± 0.376
5.281LeuPro: 5.281 ± 0.613
3.018LeuGln: 3.018 ± 0.534
6.475LeuArg: 6.475 ± 0.667
5.909LeuSer: 5.909 ± 0.497
5.846LeuThr: 5.846 ± 0.481
4.652LeuVal: 4.652 ± 0.706
1.194LeuTrp: 1.194 ± 0.292
2.075LeuTyr: 2.075 ± 0.415
0.0LeuXaa: 0.0 ± 0.0
Met
2.389MetAla: 2.389 ± 0.289
0.063MetCys: 0.063 ± 0.064
1.132MetAsp: 1.132 ± 0.274
1.572MetGlu: 1.572 ± 0.3
0.692MetPhe: 0.692 ± 0.188
1.32MetGly: 1.32 ± 0.314
0.377MetHis: 0.377 ± 0.133
0.817MetIle: 0.817 ± 0.234
1.194MetLys: 1.194 ± 0.27
1.194MetLeu: 1.194 ± 0.272
0.126MetMet: 0.126 ± 0.087
1.069MetAsn: 1.069 ± 0.242
1.006MetPro: 1.006 ± 0.242
0.44MetGln: 0.44 ± 0.171
0.88MetArg: 0.88 ± 0.257
2.137MetSer: 2.137 ± 0.376
2.577MetThr: 2.577 ± 0.334
1.069MetVal: 1.069 ± 0.337
0.251MetTrp: 0.251 ± 0.133
0.44MetTyr: 0.44 ± 0.194
0.0MetXaa: 0.0 ± 0.0
Asn
3.458AsnAla: 3.458 ± 0.479
0.0AsnCys: 0.0 ± 0.0
2.577AsnAsp: 2.577 ± 0.479
1.823AsnGlu: 1.823 ± 0.383
1.069AsnPhe: 1.069 ± 0.28
3.709AsnGly: 3.709 ± 0.581
0.754AsnHis: 0.754 ± 0.201
1.823AsnIle: 1.823 ± 0.346
0.629AsnLys: 0.629 ± 0.228
2.577AsnLeu: 2.577 ± 0.326
0.503AsnMet: 0.503 ± 0.168
0.817AsnAsn: 0.817 ± 0.179
2.703AsnPro: 2.703 ± 0.378
1.069AsnGln: 1.069 ± 0.227
1.635AsnArg: 1.635 ± 0.337
1.886AsnSer: 1.886 ± 0.389
1.635AsnThr: 1.635 ± 0.284
2.389AsnVal: 2.389 ± 0.441
0.88AsnTrp: 0.88 ± 0.241
1.006AsnTyr: 1.006 ± 0.254
0.0AsnXaa: 0.0 ± 0.0
Pro
5.344ProAla: 5.344 ± 0.57
0.314ProCys: 0.314 ± 0.148
4.338ProAsp: 4.338 ± 0.502
4.401ProGlu: 4.401 ± 0.566
2.326ProPhe: 2.326 ± 0.465
4.589ProGly: 4.589 ± 0.571
0.754ProHis: 0.754 ± 0.199
2.577ProIle: 2.577 ± 0.436
1.823ProLys: 1.823 ± 0.337
4.086ProLeu: 4.086 ± 0.557
1.132ProMet: 1.132 ± 0.269
1.697ProAsn: 1.697 ± 0.336
2.766ProPro: 2.766 ± 0.477
1.383ProGln: 1.383 ± 0.35
2.515ProArg: 2.515 ± 0.418
3.709ProSer: 3.709 ± 0.459
3.269ProThr: 3.269 ± 0.475
4.023ProVal: 4.023 ± 0.436
0.88ProTrp: 0.88 ± 0.295
1.635ProTyr: 1.635 ± 0.361
0.0ProXaa: 0.0 ± 0.0
Gln
3.018GlnAla: 3.018 ± 0.5
0.126GlnCys: 0.126 ± 0.093
1.509GlnAsp: 1.509 ± 0.381
1.635GlnGlu: 1.635 ± 0.335
1.132GlnPhe: 1.132 ± 0.251
2.263GlnGly: 2.263 ± 0.365
0.503GlnHis: 0.503 ± 0.175
2.892GlnIle: 2.892 ± 0.522
1.446GlnLys: 1.446 ± 0.352
3.52GlnLeu: 3.52 ± 0.564
0.88GlnMet: 0.88 ± 0.233
0.566GlnAsn: 0.566 ± 0.181
1.823GlnPro: 1.823 ± 0.318
1.635GlnGln: 1.635 ± 0.355
1.446GlnArg: 1.446 ± 0.386
2.075GlnSer: 2.075 ± 0.396
1.697GlnThr: 1.697 ± 0.305
2.263GlnVal: 2.263 ± 0.334
0.566GlnTrp: 0.566 ± 0.159
0.566GlnTyr: 0.566 ± 0.177
0.0GlnXaa: 0.0 ± 0.0
Arg
5.155ArgAla: 5.155 ± 0.637
1.006ArgCys: 1.006 ± 0.347
2.892ArgAsp: 2.892 ± 0.437
4.841ArgGlu: 4.841 ± 0.726
1.635ArgPhe: 1.635 ± 0.34
4.966ArgGly: 4.966 ± 0.687
1.069ArgHis: 1.069 ± 0.27
2.703ArgIle: 2.703 ± 0.487
3.52ArgLys: 3.52 ± 0.612
6.287ArgLeu: 6.287 ± 0.712
1.886ArgMet: 1.886 ± 0.348
2.64ArgAsn: 2.64 ± 0.517
2.075ArgPro: 2.075 ± 0.356
1.886ArgGln: 1.886 ± 0.375
5.029ArgArg: 5.029 ± 0.743
3.646ArgSer: 3.646 ± 0.504
2.892ArgThr: 2.892 ± 0.45
4.966ArgVal: 4.966 ± 0.492
1.069ArgTrp: 1.069 ± 0.254
1.509ArgTyr: 1.509 ± 0.248
0.0ArgXaa: 0.0 ± 0.0
Ser
6.475SerAla: 6.475 ± 0.874
0.44SerCys: 0.44 ± 0.193
3.206SerAsp: 3.206 ± 0.387
4.463SerGlu: 4.463 ± 0.509
2.137SerPhe: 2.137 ± 0.488
6.789SerGly: 6.789 ± 0.965
1.509SerHis: 1.509 ± 0.308
2.892SerIle: 2.892 ± 0.417
2.829SerLys: 2.829 ± 0.406
4.904SerLeu: 4.904 ± 0.62
1.509SerMet: 1.509 ± 0.319
2.452SerAsn: 2.452 ± 0.478
3.143SerPro: 3.143 ± 0.506
2.137SerGln: 2.137 ± 0.337
3.08SerArg: 3.08 ± 0.38
3.898SerSer: 3.898 ± 0.686
3.143SerThr: 3.143 ± 0.548
4.589SerVal: 4.589 ± 0.571
1.257SerTrp: 1.257 ± 0.33
1.509SerTyr: 1.509 ± 0.384
0.0SerXaa: 0.0 ± 0.0
Thr
5.406ThrAla: 5.406 ± 0.716
0.189ThrCys: 0.189 ± 0.113
3.961ThrAsp: 3.961 ± 0.494
4.401ThrGlu: 4.401 ± 0.526
2.137ThrPhe: 2.137 ± 0.371
6.161ThrGly: 6.161 ± 0.658
1.132ThrHis: 1.132 ± 0.317
2.955ThrIle: 2.955 ± 0.543
2.829ThrLys: 2.829 ± 0.369
5.658ThrLeu: 5.658 ± 0.642
0.88ThrMet: 0.88 ± 0.243
1.697ThrAsn: 1.697 ± 0.3
3.898ThrPro: 3.898 ± 0.573
1.509ThrGln: 1.509 ± 0.308
3.772ThrArg: 3.772 ± 0.583
3.583ThrSer: 3.583 ± 0.52
4.086ThrThr: 4.086 ± 0.617
5.029ThrVal: 5.029 ± 0.616
1.132ThrTrp: 1.132 ± 0.278
1.697ThrTyr: 1.697 ± 0.375
0.0ThrXaa: 0.0 ± 0.0
Val
6.475ValAla: 6.475 ± 0.755
0.314ValCys: 0.314 ± 0.165
5.281ValAsp: 5.281 ± 0.599
5.281ValGlu: 5.281 ± 0.599
2.515ValPhe: 2.515 ± 0.344
4.589ValGly: 4.589 ± 0.634
1.509ValHis: 1.509 ± 0.262
3.646ValIle: 3.646 ± 0.494
3.018ValLys: 3.018 ± 0.439
5.909ValLeu: 5.909 ± 0.591
1.069ValMet: 1.069 ± 0.28
2.955ValAsn: 2.955 ± 0.399
3.961ValPro: 3.961 ± 0.547
1.949ValGln: 1.949 ± 0.418
5.532ValArg: 5.532 ± 0.876
4.904ValSer: 4.904 ± 0.52
4.715ValThr: 4.715 ± 0.622
4.841ValVal: 4.841 ± 0.7
1.446ValTrp: 1.446 ± 0.321
2.515ValTyr: 2.515 ± 0.454
0.0ValXaa: 0.0 ± 0.0
Trp
1.509TrpAla: 1.509 ± 0.287
0.189TrpCys: 0.189 ± 0.109
1.383TrpAsp: 1.383 ± 0.293
0.943TrpGlu: 0.943 ± 0.221
0.88TrpPhe: 0.88 ± 0.239
1.697TrpGly: 1.697 ± 0.319
0.629TrpHis: 0.629 ± 0.207
1.32TrpIle: 1.32 ± 0.302
0.377TrpLys: 0.377 ± 0.194
1.76TrpLeu: 1.76 ± 0.347
0.44TrpMet: 0.44 ± 0.186
0.629TrpAsn: 0.629 ± 0.216
0.566TrpPro: 0.566 ± 0.198
0.817TrpGln: 0.817 ± 0.215
1.257TrpArg: 1.257 ± 0.29
1.132TrpSer: 1.132 ± 0.278
1.823TrpThr: 1.823 ± 0.35
2.075TrpVal: 2.075 ± 0.336
0.566TrpTrp: 0.566 ± 0.218
0.189TrpTyr: 0.189 ± 0.094
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.326TyrAla: 2.326 ± 0.373
0.189TyrCys: 0.189 ± 0.104
1.257TyrAsp: 1.257 ± 0.317
2.389TyrGlu: 2.389 ± 0.4
0.503TyrPhe: 0.503 ± 0.171
2.64TyrGly: 2.64 ± 0.44
0.566TyrHis: 0.566 ± 0.205
1.635TyrIle: 1.635 ± 0.312
1.509TyrLys: 1.509 ± 0.331
2.703TyrLeu: 2.703 ± 0.494
0.503TyrMet: 0.503 ± 0.15
1.132TyrAsn: 1.132 ± 0.275
1.069TyrPro: 1.069 ± 0.271
0.943TyrGln: 0.943 ± 0.241
2.577TyrArg: 2.577 ± 0.379
1.32TyrSer: 1.32 ± 0.277
1.949TyrThr: 1.949 ± 0.383
2.263TyrVal: 2.263 ± 0.446
0.503TyrTrp: 0.503 ± 0.23
0.44TyrTyr: 0.44 ± 0.162
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 89 proteins (15908 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski