Amino acid dipepetide frequency for Salmonella phage epsilon15

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
13.205AlaAla: 13.205 ± 1.637
1.047AlaCys: 1.047 ± 0.252
6.924AlaAsp: 6.924 ± 0.664
5.556AlaGlu: 5.556 ± 0.876
4.106AlaPhe: 4.106 ± 0.561
8.213AlaGly: 8.213 ± 0.79
1.288AlaHis: 1.288 ± 0.301
5.153AlaIle: 5.153 ± 0.8
4.187AlaLys: 4.187 ± 0.474
8.293AlaLeu: 8.293 ± 0.891
3.462AlaMet: 3.462 ± 0.465
4.428AlaAsn: 4.428 ± 0.72
4.187AlaPro: 4.187 ± 0.898
5.958AlaGln: 5.958 ± 1.109
6.2AlaArg: 6.2 ± 0.956
5.878AlaSer: 5.878 ± 0.753
4.348AlaThr: 4.348 ± 0.613
6.441AlaVal: 6.441 ± 0.715
1.53AlaTrp: 1.53 ± 0.329
3.865AlaTyr: 3.865 ± 0.947
0.0AlaXaa: 0.0 ± 0.0
Cys
0.966CysAla: 0.966 ± 0.252
0.0CysCys: 0.0 ± 0.0
0.483CysAsp: 0.483 ± 0.202
0.805CysGlu: 0.805 ± 0.229
0.403CysPhe: 0.403 ± 0.212
0.644CysGly: 0.644 ± 0.248
0.161CysHis: 0.161 ± 0.107
0.805CysIle: 0.805 ± 0.283
0.322CysLys: 0.322 ± 0.188
0.403CysLeu: 0.403 ± 0.2
0.242CysMet: 0.242 ± 0.137
0.483CysAsn: 0.483 ± 0.232
0.564CysPro: 0.564 ± 0.212
0.242CysGln: 0.242 ± 0.139
0.725CysArg: 0.725 ± 0.235
0.886CysSer: 0.886 ± 0.314
0.483CysThr: 0.483 ± 0.197
0.805CysVal: 0.805 ± 0.281
0.161CysTrp: 0.161 ± 0.12
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
6.763AspAla: 6.763 ± 0.907
0.564AspCys: 0.564 ± 0.231
4.831AspAsp: 4.831 ± 0.605
3.14AspGlu: 3.14 ± 0.548
2.013AspPhe: 2.013 ± 0.327
5.395AspGly: 5.395 ± 0.655
0.966AspHis: 0.966 ± 0.256
3.623AspIle: 3.623 ± 0.442
3.301AspLys: 3.301 ± 0.651
4.831AspLeu: 4.831 ± 0.458
1.288AspMet: 1.288 ± 0.329
2.899AspAsn: 2.899 ± 0.61
2.013AspPro: 2.013 ± 0.446
2.738AspGln: 2.738 ± 0.453
3.221AspArg: 3.221 ± 0.7
3.301AspSer: 3.301 ± 0.463
2.818AspThr: 2.818 ± 0.383
4.831AspVal: 4.831 ± 0.722
1.208AspTrp: 1.208 ± 0.407
1.691AspTyr: 1.691 ± 0.382
0.0AspXaa: 0.0 ± 0.0
Glu
5.233GluAla: 5.233 ± 0.632
0.242GluCys: 0.242 ± 0.125
3.382GluAsp: 3.382 ± 0.575
3.865GluGlu: 3.865 ± 0.628
2.576GluPhe: 2.576 ± 0.329
2.899GluGly: 2.899 ± 0.428
0.886GluHis: 0.886 ± 0.282
2.979GluIle: 2.979 ± 0.641
4.106GluLys: 4.106 ± 0.699
5.233GluLeu: 5.233 ± 0.836
1.61GluMet: 1.61 ± 0.354
1.691GluAsn: 1.691 ± 0.387
2.174GluPro: 2.174 ± 0.41
3.945GluGln: 3.945 ± 0.543
3.865GluArg: 3.865 ± 0.914
3.06GluSer: 3.06 ± 0.766
3.301GluThr: 3.301 ± 0.488
3.704GluVal: 3.704 ± 0.668
0.725GluTrp: 0.725 ± 0.213
2.254GluTyr: 2.254 ± 0.329
0.0GluXaa: 0.0 ± 0.0
Phe
3.462PheAla: 3.462 ± 0.65
0.564PheCys: 0.564 ± 0.205
2.657PheAsp: 2.657 ± 0.414
2.013PheGlu: 2.013 ± 0.334
1.127PhePhe: 1.127 ± 0.3
3.14PheGly: 3.14 ± 0.462
0.242PheHis: 0.242 ± 0.18
2.899PheIle: 2.899 ± 0.493
2.415PheLys: 2.415 ± 0.418
2.174PheLeu: 2.174 ± 0.664
0.886PheMet: 0.886 ± 0.216
1.691PheAsn: 1.691 ± 0.241
1.288PhePro: 1.288 ± 0.355
0.966PheGln: 0.966 ± 0.313
1.932PheArg: 1.932 ± 0.354
2.899PheSer: 2.899 ± 0.475
2.496PheThr: 2.496 ± 0.336
1.932PheVal: 1.932 ± 0.427
0.483PheTrp: 0.483 ± 0.151
0.886PheTyr: 0.886 ± 0.282
0.0PheXaa: 0.0 ± 0.0
Gly
4.911GlyAla: 4.911 ± 0.709
0.644GlyCys: 0.644 ± 0.216
4.589GlyAsp: 4.589 ± 0.517
4.509GlyGlu: 4.509 ± 0.555
3.704GlyPhe: 3.704 ± 0.55
5.797GlyGly: 5.797 ± 0.808
1.852GlyHis: 1.852 ± 0.353
5.072GlyIle: 5.072 ± 0.639
4.67GlyLys: 4.67 ± 0.521
5.958GlyLeu: 5.958 ± 0.682
2.174GlyMet: 2.174 ± 0.374
4.187GlyAsn: 4.187 ± 1.009
1.53GlyPro: 1.53 ± 0.392
3.462GlyGln: 3.462 ± 0.629
3.865GlyArg: 3.865 ± 0.599
4.911GlySer: 4.911 ± 0.531
4.026GlyThr: 4.026 ± 0.553
4.509GlyVal: 4.509 ± 0.662
1.53GlyTrp: 1.53 ± 0.346
2.093GlyTyr: 2.093 ± 0.335
0.0GlyXaa: 0.0 ± 0.0
His
1.127HisAla: 1.127 ± 0.347
0.242HisCys: 0.242 ± 0.144
0.725HisAsp: 0.725 ± 0.208
1.449HisGlu: 1.449 ± 0.359
0.483HisPhe: 0.483 ± 0.191
1.449HisGly: 1.449 ± 0.343
0.242HisHis: 0.242 ± 0.116
1.127HisIle: 1.127 ± 0.33
0.644HisLys: 0.644 ± 0.257
1.369HisLeu: 1.369 ± 0.397
0.644HisMet: 0.644 ± 0.197
1.047HisAsn: 1.047 ± 0.323
0.644HisPro: 0.644 ± 0.183
0.242HisGln: 0.242 ± 0.124
0.966HisArg: 0.966 ± 0.268
1.53HisSer: 1.53 ± 0.371
0.886HisThr: 0.886 ± 0.272
0.966HisVal: 0.966 ± 0.296
0.483HisTrp: 0.483 ± 0.235
0.644HisTyr: 0.644 ± 0.237
0.0HisXaa: 0.0 ± 0.0
Ile
5.153IleAla: 5.153 ± 0.751
0.725IleCys: 0.725 ± 0.258
3.301IleAsp: 3.301 ± 0.401
2.657IleGlu: 2.657 ± 0.377
2.013IlePhe: 2.013 ± 0.574
4.911IleGly: 4.911 ± 0.449
0.886IleHis: 0.886 ± 0.272
2.979IleIle: 2.979 ± 0.682
2.818IleLys: 2.818 ± 0.533
3.14IleLeu: 3.14 ± 0.716
1.127IleMet: 1.127 ± 0.304
3.301IleAsn: 3.301 ± 0.45
3.14IlePro: 3.14 ± 0.633
2.254IleGln: 2.254 ± 0.468
3.14IleArg: 3.14 ± 0.406
4.348IleSer: 4.348 ± 0.712
4.67IleThr: 4.67 ± 0.753
3.06IleVal: 3.06 ± 0.519
1.208IleTrp: 1.208 ± 0.274
2.013IleTyr: 2.013 ± 0.466
0.0IleXaa: 0.0 ± 0.0
Lys
5.153LysAla: 5.153 ± 0.934
0.725LysCys: 0.725 ± 0.226
1.852LysAsp: 1.852 ± 0.366
2.818LysGlu: 2.818 ± 0.556
1.288LysPhe: 1.288 ± 0.298
2.818LysGly: 2.818 ± 0.382
1.047LysHis: 1.047 ± 0.26
2.415LysIle: 2.415 ± 0.415
2.576LysLys: 2.576 ± 0.501
4.67LysLeu: 4.67 ± 0.723
1.53LysMet: 1.53 ± 0.396
2.415LysAsn: 2.415 ± 0.522
3.221LysPro: 3.221 ± 0.694
2.093LysGln: 2.093 ± 0.406
3.221LysArg: 3.221 ± 0.642
3.06LysSer: 3.06 ± 0.569
3.221LysThr: 3.221 ± 0.541
3.14LysVal: 3.14 ± 0.449
0.886LysTrp: 0.886 ± 0.294
1.691LysTyr: 1.691 ± 0.334
0.0LysXaa: 0.0 ± 0.0
Leu
8.293LeuAla: 8.293 ± 0.725
0.725LeuCys: 0.725 ± 0.25
4.348LeuAsp: 4.348 ± 0.608
4.187LeuGlu: 4.187 ± 0.778
3.301LeuPhe: 3.301 ± 0.593
5.072LeuGly: 5.072 ± 0.651
1.53LeuHis: 1.53 ± 0.42
5.314LeuIle: 5.314 ± 0.839
3.704LeuLys: 3.704 ± 0.61
5.878LeuLeu: 5.878 ± 0.716
1.53LeuMet: 1.53 ± 0.383
3.462LeuAsn: 3.462 ± 0.585
3.06LeuPro: 3.06 ± 0.374
3.14LeuGln: 3.14 ± 0.396
4.589LeuArg: 4.589 ± 0.62
6.361LeuSer: 6.361 ± 0.779
5.395LeuThr: 5.395 ± 0.594
4.187LeuVal: 4.187 ± 0.578
0.483LeuTrp: 0.483 ± 0.184
2.174LeuTyr: 2.174 ± 0.443
0.0LeuXaa: 0.0 ± 0.0
Met
3.623MetAla: 3.623 ± 0.742
0.242MetCys: 0.242 ± 0.142
1.288MetAsp: 1.288 ± 0.356
0.886MetGlu: 0.886 ± 0.272
1.208MetPhe: 1.208 ± 0.288
1.449MetGly: 1.449 ± 0.456
0.242MetHis: 0.242 ± 0.171
1.127MetIle: 1.127 ± 0.356
2.093MetLys: 2.093 ± 0.543
2.496MetLeu: 2.496 ± 0.471
1.208MetMet: 1.208 ± 0.336
1.127MetAsn: 1.127 ± 0.309
1.369MetPro: 1.369 ± 0.456
0.966MetGln: 0.966 ± 0.239
1.047MetArg: 1.047 ± 0.263
2.576MetSer: 2.576 ± 0.451
2.093MetThr: 2.093 ± 0.466
1.932MetVal: 1.932 ± 0.36
0.242MetTrp: 0.242 ± 0.126
0.483MetTyr: 0.483 ± 0.205
0.0MetXaa: 0.0 ± 0.0
Asn
4.992AsnAla: 4.992 ± 0.702
0.081AsnCys: 0.081 ± 0.092
2.657AsnAsp: 2.657 ± 0.409
2.818AsnGlu: 2.818 ± 0.404
1.127AsnPhe: 1.127 ± 0.321
4.428AsnGly: 4.428 ± 0.921
0.564AsnHis: 0.564 ± 0.188
2.496AsnIle: 2.496 ± 0.507
2.093AsnLys: 2.093 ± 0.416
3.14AsnLeu: 3.14 ± 0.506
0.966AsnMet: 0.966 ± 0.284
2.738AsnAsn: 2.738 ± 0.998
3.06AsnPro: 3.06 ± 0.564
1.932AsnGln: 1.932 ± 0.395
2.657AsnArg: 2.657 ± 0.456
2.899AsnSer: 2.899 ± 0.566
2.818AsnThr: 2.818 ± 0.498
2.415AsnVal: 2.415 ± 0.397
0.483AsnTrp: 0.483 ± 0.232
1.852AsnTyr: 1.852 ± 0.571
0.0AsnXaa: 0.0 ± 0.0
Pro
4.267ProAla: 4.267 ± 1.01
0.322ProCys: 0.322 ± 0.16
3.543ProAsp: 3.543 ± 0.744
3.301ProGlu: 3.301 ± 0.568
1.449ProPhe: 1.449 ± 0.293
2.818ProGly: 2.818 ± 0.356
0.725ProHis: 0.725 ± 0.309
1.61ProIle: 1.61 ± 0.304
1.53ProLys: 1.53 ± 0.426
2.415ProLeu: 2.415 ± 0.43
0.564ProMet: 0.564 ± 0.212
1.047ProAsn: 1.047 ± 0.299
1.932ProPro: 1.932 ± 0.479
2.576ProGln: 2.576 ± 0.398
1.53ProArg: 1.53 ± 0.325
2.415ProSer: 2.415 ± 0.629
2.899ProThr: 2.899 ± 0.546
3.865ProVal: 3.865 ± 0.537
0.886ProTrp: 0.886 ± 0.318
1.369ProTyr: 1.369 ± 0.353
0.0ProXaa: 0.0 ± 0.0
Gln
6.2GlnAla: 6.2 ± 1.357
0.725GlnCys: 0.725 ± 0.276
2.335GlnAsp: 2.335 ± 0.353
2.818GlnGlu: 2.818 ± 0.5
1.932GlnPhe: 1.932 ± 0.33
2.174GlnGly: 2.174 ± 0.448
0.805GlnHis: 0.805 ± 0.289
1.61GlnIle: 1.61 ± 0.45
1.771GlnLys: 1.771 ± 0.455
4.187GlnLeu: 4.187 ± 0.73
1.208GlnMet: 1.208 ± 0.306
1.61GlnAsn: 1.61 ± 0.349
2.496GlnPro: 2.496 ± 0.505
3.382GlnGln: 3.382 ± 0.8
3.301GlnArg: 3.301 ± 0.375
2.415GlnSer: 2.415 ± 0.563
2.335GlnThr: 2.335 ± 0.573
3.221GlnVal: 3.221 ± 0.544
0.644GlnTrp: 0.644 ± 0.19
2.496GlnTyr: 2.496 ± 0.459
0.0GlnXaa: 0.0 ± 0.0
Arg
6.039ArgAla: 6.039 ± 0.87
0.564ArgCys: 0.564 ± 0.222
3.865ArgAsp: 3.865 ± 0.516
3.301ArgGlu: 3.301 ± 0.589
1.369ArgPhe: 1.369 ± 0.245
3.462ArgGly: 3.462 ± 0.465
0.805ArgHis: 0.805 ± 0.214
3.945ArgIle: 3.945 ± 0.462
3.382ArgLys: 3.382 ± 0.494
4.187ArgLeu: 4.187 ± 0.721
2.496ArgMet: 2.496 ± 0.486
2.738ArgAsn: 2.738 ± 0.346
1.047ArgPro: 1.047 ± 0.317
4.348ArgGln: 4.348 ± 0.793
3.462ArgArg: 3.462 ± 0.651
2.335ArgSer: 2.335 ± 0.411
2.335ArgThr: 2.335 ± 0.373
4.026ArgVal: 4.026 ± 0.611
0.886ArgTrp: 0.886 ± 0.276
2.093ArgTyr: 2.093 ± 0.432
0.0ArgXaa: 0.0 ± 0.0
Ser
7.89SerAla: 7.89 ± 0.802
0.644SerCys: 0.644 ± 0.224
3.784SerAsp: 3.784 ± 0.66
4.509SerGlu: 4.509 ± 0.593
2.576SerPhe: 2.576 ± 0.497
5.153SerGly: 5.153 ± 0.767
1.127SerHis: 1.127 ± 0.227
3.704SerIle: 3.704 ± 0.548
3.06SerLys: 3.06 ± 0.33
4.911SerLeu: 4.911 ± 0.691
2.254SerMet: 2.254 ± 0.444
3.06SerAsn: 3.06 ± 0.492
2.335SerPro: 2.335 ± 0.356
2.174SerGln: 2.174 ± 0.497
3.865SerArg: 3.865 ± 0.495
5.233SerSer: 5.233 ± 0.862
4.106SerThr: 4.106 ± 0.508
4.026SerVal: 4.026 ± 0.639
0.725SerTrp: 0.725 ± 0.201
1.449SerTyr: 1.449 ± 0.273
0.0SerXaa: 0.0 ± 0.0
Thr
6.683ThrAla: 6.683 ± 0.653
0.483ThrCys: 0.483 ± 0.2
4.267ThrAsp: 4.267 ± 0.786
2.738ThrGlu: 2.738 ± 0.638
1.61ThrPhe: 1.61 ± 0.341
6.522ThrGly: 6.522 ± 0.67
1.047ThrHis: 1.047 ± 0.272
2.979ThrIle: 2.979 ± 0.495
2.415ThrLys: 2.415 ± 0.501
4.75ThrLeu: 4.75 ± 0.554
1.288ThrMet: 1.288 ± 0.336
2.657ThrAsn: 2.657 ± 0.299
2.899ThrPro: 2.899 ± 0.422
2.335ThrGln: 2.335 ± 0.46
2.174ThrArg: 2.174 ± 0.383
4.67ThrSer: 4.67 ± 0.847
3.704ThrThr: 3.704 ± 0.623
3.543ThrVal: 3.543 ± 0.681
1.047ThrTrp: 1.047 ± 0.315
1.369ThrTyr: 1.369 ± 0.364
0.0ThrXaa: 0.0 ± 0.0
Val
6.2ValAla: 6.2 ± 0.686
0.403ValCys: 0.403 ± 0.163
4.026ValAsp: 4.026 ± 0.634
3.945ValGlu: 3.945 ± 0.648
2.254ValPhe: 2.254 ± 0.429
4.267ValGly: 4.267 ± 0.616
1.61ValHis: 1.61 ± 0.331
4.428ValIle: 4.428 ± 0.673
2.496ValLys: 2.496 ± 0.612
4.267ValLeu: 4.267 ± 0.548
2.013ValMet: 2.013 ± 0.424
3.382ValAsn: 3.382 ± 0.544
2.093ValPro: 2.093 ± 0.44
2.576ValGln: 2.576 ± 0.457
4.187ValArg: 4.187 ± 0.425
4.428ValSer: 4.428 ± 0.691
4.267ValThr: 4.267 ± 0.499
4.75ValVal: 4.75 ± 0.784
1.127ValTrp: 1.127 ± 0.325
1.852ValTyr: 1.852 ± 0.313
0.0ValXaa: 0.0 ± 0.0
Trp
0.886TrpAla: 0.886 ± 0.32
0.242TrpCys: 0.242 ± 0.12
0.644TrpAsp: 0.644 ± 0.203
0.725TrpGlu: 0.725 ± 0.259
0.805TrpPhe: 0.805 ± 0.212
1.208TrpGly: 1.208 ± 0.374
0.242TrpHis: 0.242 ± 0.135
0.725TrpIle: 0.725 ± 0.299
0.725TrpLys: 0.725 ± 0.299
2.174TrpLeu: 2.174 ± 0.515
0.322TrpMet: 0.322 ± 0.146
0.644TrpAsn: 0.644 ± 0.25
0.403TrpPro: 0.403 ± 0.24
0.805TrpGln: 0.805 ± 0.264
0.725TrpArg: 0.725 ± 0.291
1.288TrpSer: 1.288 ± 0.335
0.644TrpThr: 0.644 ± 0.219
1.208TrpVal: 1.208 ± 0.3
0.161TrpTrp: 0.161 ± 0.1
0.644TrpTyr: 0.644 ± 0.232
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.221TyrAla: 3.221 ± 0.444
0.403TyrCys: 0.403 ± 0.168
1.932TyrAsp: 1.932 ± 0.407
1.771TyrGlu: 1.771 ± 0.461
0.805TyrPhe: 0.805 ± 0.309
2.174TyrGly: 2.174 ± 0.281
0.644TyrHis: 0.644 ± 0.219
1.852TyrIle: 1.852 ± 0.475
1.53TyrLys: 1.53 ± 0.338
2.254TyrLeu: 2.254 ± 0.535
0.805TyrMet: 0.805 ± 0.251
1.61TyrAsn: 1.61 ± 0.41
1.61TyrPro: 1.61 ± 0.454
1.53TyrGln: 1.53 ± 0.316
2.013TyrArg: 2.013 ± 0.343
2.093TyrSer: 2.093 ± 0.383
2.335TyrThr: 2.335 ± 0.444
2.013TyrVal: 2.013 ± 0.323
0.322TyrTrp: 0.322 ± 0.158
0.725TyrTyr: 0.725 ± 0.245
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 51 proteins (12421 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski