Frog virus 3 (isolate Goorha) (FV-3)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Megaviricetes; Pimascovirales; Iridoviridae; Alphairidovirinae; Ranavirus; Frog virus 3

Average proteome isoelectric point is 7.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 98 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q6GZN3|092R_FRG3G Uncharacterized protein 092R OS=Frog virus 3 (isolate Goorha) OX=654924 GN=FV3-092R PE=4 SV=1
MM1 pKa = 8.17DD2 pKa = 4.92KK3 pKa = 9.58PTVEE7 pKa = 4.01TSAAPVEE14 pKa = 4.38TLVLTEE20 pKa = 4.99PPAEE24 pKa = 4.21TQAEE28 pKa = 4.21DD29 pKa = 3.62SVSSVLAGLTAAIEE43 pKa = 4.33TVDD46 pKa = 3.64RR47 pKa = 11.84LRR49 pKa = 11.84TAFGAEE55 pKa = 3.81

Molecular weight: 5.69 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q67472|DNMT_FRG3G Putative DNA (cytosine-5)-methyltransferase OS=Frog virus 3 (isolate Goorha) OX=654924 GN=FV3-083R PE=3 SV=1
MM1 pKa = 7.73LLHH4 pKa = 7.32LILILKK10 pKa = 10.2SFLPKK15 pKa = 10.05RR16 pKa = 11.84NLAFRR21 pKa = 11.84YY22 pKa = 10.25DD23 pKa = 3.59IVALSLTRR31 pKa = 11.84KK32 pKa = 9.43ILLKK36 pKa = 10.3HH37 pKa = 5.52QPPEE41 pKa = 3.88NVFAALYY48 pKa = 9.96IDD50 pKa = 4.0SLHH53 pKa = 6.61GGRR56 pKa = 11.84HH57 pKa = 4.41QIMPGFCKK65 pKa = 10.62LSCPTLDD72 pKa = 3.31VGPGLGRR79 pKa = 11.84FAASHH84 pKa = 5.59FSEE87 pKa = 5.14RR88 pKa = 11.84VSQDD92 pKa = 2.44RR93 pKa = 11.84QAQGRR98 pKa = 11.84EE99 pKa = 3.88RR100 pKa = 11.84RR101 pKa = 11.84SVLLPQEE108 pKa = 4.3RR109 pKa = 11.84RR110 pKa = 11.84GSSGRR115 pKa = 11.84QPLYY119 pKa = 10.27SLLPHH124 pKa = 6.15RR125 pKa = 11.84PKK127 pKa = 10.66RR128 pKa = 11.84EE129 pKa = 3.88GVIGAALCYY138 pKa = 9.71TSASHH143 pKa = 6.32SLSPAALLPPVGRR156 pKa = 11.84GKK158 pKa = 10.32DD159 pKa = 3.44RR160 pKa = 11.84RR161 pKa = 11.84CGRR164 pKa = 11.84QVGAAGLLRR173 pKa = 11.84HH174 pKa = 5.84VGRR177 pKa = 11.84RR178 pKa = 11.84RR179 pKa = 11.84QFAQLTRR186 pKa = 11.84GPLGLPCALLEE197 pKa = 4.24PLPLDD202 pKa = 3.33PHH204 pKa = 7.85LLDD207 pKa = 3.87KK208 pKa = 11.36GRR210 pKa = 11.84DD211 pKa = 3.52VGVGGCHH218 pKa = 6.38DD219 pKa = 3.93HH220 pKa = 7.14QGQEE224 pKa = 4.61DD225 pKa = 4.34GLLLHH230 pKa = 6.99GGPEE234 pKa = 4.27PVLGPQGVIGPAYY247 pKa = 9.75GRR249 pKa = 11.84PHH251 pKa = 6.9ANVHH255 pKa = 5.58ALRR258 pKa = 11.84HH259 pKa = 5.24EE260 pKa = 4.42LQHH263 pKa = 6.74GPLGDD268 pKa = 3.84PPRR271 pKa = 11.84LRR273 pKa = 11.84SGG275 pKa = 3.21

Molecular weight: 29.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

98

0

98

28355

50

1293

289.3

31.98

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.686 ± 0.283

2.271 ± 0.219

6.013 ± 0.153

5.569 ± 0.205

3.213 ± 0.129

7.533 ± 0.223

2.201 ± 0.153

3.227 ± 0.157

5.47 ± 0.241

8.055 ± 0.277

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.008 ± 0.138

2.62 ± 0.147

6.44 ± 0.334

3.008 ± 0.272

7.145 ± 0.354

7.254 ± 0.224

5.438 ± 0.221

8.305 ± 0.24

1.594 ± 0.137

2.948 ± 0.14
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski