Tibrogargan virus (strain CS132) (TIBV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Tibrovirus; Tibrogargan tibrovirus; Tibrogargan virus

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 9 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|D8V072|MATRX_TIBVC Matrix protein OS=Tibrogargan virus (strain CS132) OX=1559361 GN=M PE=3 SV=1
MM1 pKa = 7.18KK2 pKa = 9.89RR3 pKa = 11.84HH4 pKa = 6.68RR5 pKa = 11.84ISIPYY10 pKa = 8.38VTDD13 pKa = 3.12QVLRR17 pKa = 11.84NTSDD21 pKa = 3.44VVDD24 pKa = 4.58PNDD27 pKa = 4.1TVDD30 pKa = 4.27QLISDD35 pKa = 4.14DD36 pKa = 4.05VVNPKK41 pKa = 9.92QDD43 pKa = 3.31LKK45 pKa = 11.26EE46 pKa = 4.06FLDD49 pKa = 3.88SRR51 pKa = 11.84EE52 pKa = 3.88LNYY55 pKa = 9.44RR56 pKa = 11.84TRR58 pKa = 11.84ASLASSYY65 pKa = 11.65DD66 pKa = 3.48DD67 pKa = 5.93DD68 pKa = 4.99DD69 pKa = 4.0WADD72 pKa = 3.86SIVDD76 pKa = 3.86LSQRR80 pKa = 11.84PHH82 pKa = 6.85NKK84 pKa = 8.65MEE86 pKa = 4.16EE87 pKa = 4.22SSLHH91 pKa = 6.42DD92 pKa = 4.4DD93 pKa = 4.14KK94 pKa = 11.55AIKK97 pKa = 9.95QATQLNTDD105 pKa = 3.88YY106 pKa = 11.35NQLRR110 pKa = 11.84SPNANSIGGQSVIKK124 pKa = 10.42DD125 pKa = 3.39VSSEE129 pKa = 3.97RR130 pKa = 11.84KK131 pKa = 9.47PPVNQIPEE139 pKa = 4.12DD140 pKa = 3.67QQIYY144 pKa = 8.94PSQMYY149 pKa = 9.45PLLEE153 pKa = 4.25VPEE156 pKa = 5.12SYY158 pKa = 10.64HH159 pKa = 7.85SLIPKK164 pKa = 9.31LQFFFKK170 pKa = 10.87YY171 pKa = 9.72YY172 pKa = 11.07GLYY175 pKa = 9.84EE176 pKa = 4.67DD177 pKa = 4.21SDD179 pKa = 4.56YY180 pKa = 11.88VVDD183 pKa = 5.45KK184 pKa = 11.1DD185 pKa = 3.49NQGYY189 pKa = 8.78YY190 pKa = 10.35FYY192 pKa = 8.72PTKK195 pKa = 10.51KK196 pKa = 7.37WTTRR200 pKa = 11.84DD201 pKa = 3.34QKK203 pKa = 11.58EE204 pKa = 3.99LMDD207 pKa = 5.23NISKK211 pKa = 10.85GVDD214 pKa = 3.11HH215 pKa = 8.08DD216 pKa = 5.77DD217 pKa = 3.64NLLDD221 pKa = 4.81LEE223 pKa = 4.96EE224 pKa = 4.39KK225 pKa = 10.33TSDD228 pKa = 3.64NLFEE232 pKa = 4.93EE233 pKa = 4.6NLNVHH238 pKa = 5.69QLVDD242 pKa = 4.37FVTKK246 pKa = 10.38GFYY249 pKa = 9.7VEE251 pKa = 4.08KK252 pKa = 10.68RR253 pKa = 11.84NIKK256 pKa = 9.31GKK258 pKa = 10.94YY259 pKa = 9.03YY260 pKa = 10.74FDD262 pKa = 3.95INNPSLNINKK272 pKa = 9.22IANVDD277 pKa = 3.85CQDD280 pKa = 4.37KK281 pKa = 10.8ILSAKK286 pKa = 9.92EE287 pKa = 3.87KK288 pKa = 10.13IDD290 pKa = 4.06MIFKK294 pKa = 10.68ASGIYY299 pKa = 9.93RR300 pKa = 11.84AMKK303 pKa = 10.51LKK305 pKa = 10.82AKK307 pKa = 8.7WW308 pKa = 3.09

Molecular weight: 35.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|D8V078|L_TIBVC RNA-directed RNA polymerase L OS=Tibrogargan virus (strain CS132) OX=1559361 GN=L PE=3 SV=1
MM1 pKa = 7.39SKK3 pKa = 10.39VGPNPFLQFYY13 pKa = 10.65LNAKK17 pKa = 9.69NDD19 pKa = 4.19FINWVSIIWGKK30 pKa = 10.14IRR32 pKa = 11.84MIALVITLIVAFIFLIKK49 pKa = 10.37LIKK52 pKa = 9.81SCIYY56 pKa = 10.43LVSLCKK62 pKa = 10.63GCLTKK67 pKa = 10.32TLTFKK72 pKa = 11.24NKK74 pKa = 9.39IIKK77 pKa = 8.47WNIWKK82 pKa = 9.84KK83 pKa = 9.6IKK85 pKa = 10.28RR86 pKa = 11.84RR87 pKa = 11.84TPRR90 pKa = 11.84EE91 pKa = 3.99SRR93 pKa = 11.84LKK95 pKa = 10.64DD96 pKa = 3.34CPIYY100 pKa = 10.84LNNPNFQLNN109 pKa = 3.44

Molecular weight: 12.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

9

0

9

4253

55

2119

472.6

54.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.151 ± 0.263

2.234 ± 0.299

6.49 ± 0.763

5.055 ± 0.223

4.397 ± 0.291

4.797 ± 0.412

2.68 ± 0.503

8.229 ± 0.382

7.312 ± 0.536

10.087 ± 0.831

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.398 ± 0.289

6.889 ± 0.246

3.833 ± 0.2

3.48 ± 0.316

4.209 ± 0.192

7.83 ± 0.518

5.667 ± 0.467

4.914 ± 0.277

1.669 ± 0.118

4.679 ± 0.18
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski