Tissierella creatinini

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Tissierellia; Tissierellales; Tissierellaceae; Tissierella

Average proteome isoelectric point is 6.22

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2472 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A4T9WIG4|A0A4T9WIG4_9FIRM DUF5067 domain-containing protein OS=Tissierella creatinini OX=43143 GN=E9840_01910 PE=4 SV=1
MM1 pKa = 7.18SACTAANDD9 pKa = 3.66SDD11 pKa = 5.43LEE13 pKa = 4.22EE14 pKa = 4.14EE15 pKa = 4.27LQAKK19 pKa = 9.91KK20 pKa = 10.58EE21 pKa = 4.06IIATLEE27 pKa = 4.03SEE29 pKa = 4.16NEE31 pKa = 3.91KK32 pKa = 10.76LQEE35 pKa = 4.85EE36 pKa = 4.4INKK39 pKa = 9.7LNNRR43 pKa = 11.84IEE45 pKa = 4.16EE46 pKa = 4.53LEE48 pKa = 4.36GEE50 pKa = 4.64TPSQTQATSYY60 pKa = 9.3LTAALEE66 pKa = 4.1AVEE69 pKa = 5.35LLSNDD74 pKa = 4.04DD75 pKa = 3.95MNGLASMAHH84 pKa = 5.71PVKK87 pKa = 10.67GIRR90 pKa = 11.84FTPYY94 pKa = 10.53SYY96 pKa = 10.84IDD98 pKa = 3.46TSTDD102 pKa = 3.24QVFTANQISNLMTDD116 pKa = 3.32TTVYY120 pKa = 9.26TWGNYY125 pKa = 9.41DD126 pKa = 4.09GSGEE130 pKa = 4.34PINKK134 pKa = 8.37TFADD138 pKa = 4.29YY139 pKa = 11.31YY140 pKa = 11.39DD141 pKa = 3.66EE142 pKa = 4.71FVYY145 pKa = 10.99DD146 pKa = 3.62EE147 pKa = 5.91DD148 pKa = 4.14YY149 pKa = 11.59ANPHH153 pKa = 6.59IIGNNTLIGKK163 pKa = 9.29GNSIDD168 pKa = 3.93NLTDD172 pKa = 2.98VYY174 pKa = 11.24TNGEE178 pKa = 3.87FVEE181 pKa = 4.85FHH183 pKa = 5.67FTGFDD188 pKa = 3.23AQYY191 pKa = 11.32EE192 pKa = 4.43GMDD195 pKa = 3.33WSSLKK200 pKa = 10.83LVFEE204 pKa = 4.58EE205 pKa = 6.21DD206 pKa = 2.97SGIWYY211 pKa = 9.45LVAIIHH217 pKa = 5.55SQWTII222 pKa = 2.94

Molecular weight:
24.99 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A4T9WA50|A0A4T9WA50_9FIRM Uncharacterized protein OS=Tissierella creatinini OX=43143 GN=E9840_12540 PE=4 SV=1
RR1 pKa = 7.14NGKK4 pKa = 6.76TLEE7 pKa = 4.17SRR9 pKa = 11.84SGEE12 pKa = 3.8AWSEE16 pKa = 3.82GFIRR20 pKa = 11.84DD21 pKa = 3.85LEE23 pKa = 5.37AITKK27 pKa = 7.1MTFKK31 pKa = 10.5RR32 pKa = 11.84RR33 pKa = 11.84NGGRR37 pKa = 11.84NKK39 pKa = 10.07HH40 pKa = 5.44GRR42 pKa = 11.84GHH44 pKa = 5.86VKK46 pKa = 10.46FIRR49 pKa = 11.84CSNCGKK55 pKa = 10.17CCPKK59 pKa = 10.69DD60 pKa = 3.53KK61 pKa = 10.6AIKK64 pKa = 10.12RR65 pKa = 11.84FLVRR69 pKa = 11.84NIVEE73 pKa = 4.03QAAVRR78 pKa = 11.84DD79 pKa = 4.08VQEE82 pKa = 3.95ACAFEE87 pKa = 5.95SYY89 pKa = 9.58TLPKK93 pKa = 10.3LYY95 pKa = 11.06VKK97 pKa = 9.54MQYY100 pKa = 10.13CVSCAIHH107 pKa = 6.07SKK109 pKa = 8.2VVRR112 pKa = 11.84VRR114 pKa = 11.84SRR116 pKa = 11.84TDD118 pKa = 2.68RR119 pKa = 11.84RR120 pKa = 11.84NRR122 pKa = 11.84EE123 pKa = 3.46PPQRR127 pKa = 11.84FRR129 pKa = 11.84RR130 pKa = 11.84RR131 pKa = 11.84DD132 pKa = 3.51DD133 pKa = 3.48AQKK136 pKa = 10.34PGQAPRR142 pKa = 11.84LAGAAGPPRR151 pKa = 11.84PP152 pKa = 4.07

Molecular weight:
17.39 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2472

0

2472

744290

22

1444

301.1

33.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.904 ± 0.052

0.925 ± 0.018

5.995 ± 0.044

7.55 ± 0.058

4.183 ± 0.037

6.96 ± 0.046

1.534 ± 0.019

9.583 ± 0.057

7.986 ± 0.05

9.511 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.731 ± 0.022

5.438 ± 0.034

3.219 ± 0.023

2.457 ± 0.022

4.003 ± 0.028

6.122 ± 0.037

4.842 ± 0.031

6.384 ± 0.048

0.672 ± 0.015

4.002 ± 0.033

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski