Tupaia virus (isolate Tupaia/Thailand/-/1986) (TUPV)

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Rhabdoviridae; Alpharhabdovirinae; Tupavirus; Tupaia tupavirus; Tupaia virus

Average proteome isoelectric point is 7.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|Q4VKV8|NCAP_TUPVT Nucleoprotein OS=Tupaia virus (isolate Tupaia/Thailand/-/1986) OX=1560034 GN=N PE=3 SV=1
MM1 pKa = 7.91TDD3 pKa = 3.16PKK5 pKa = 10.55KK6 pKa = 10.35ISRR9 pKa = 11.84VALKK13 pKa = 10.56GYY15 pKa = 10.64DD16 pKa = 3.37LTKK19 pKa = 10.46VSRR22 pKa = 11.84ALEE25 pKa = 4.13EE26 pKa = 5.54CEE28 pKa = 4.06DD29 pKa = 3.77QPGVRR34 pKa = 11.84VGGVQIVSGGEE45 pKa = 3.81AEE47 pKa = 4.69APAHH51 pKa = 6.93LEE53 pKa = 4.05DD54 pKa = 4.25NRR56 pKa = 11.84QGAIGDD62 pKa = 3.76NFDD65 pKa = 3.29VFSRR69 pKa = 11.84DD70 pKa = 3.26SKK72 pKa = 10.72NVQIVTEE79 pKa = 4.68HH80 pKa = 6.91EE81 pKa = 4.26DD82 pKa = 3.54SSDD85 pKa = 3.57EE86 pKa = 4.2EE87 pKa = 4.5YY88 pKa = 11.26EE89 pKa = 4.07EE90 pKa = 4.2ARR92 pKa = 11.84SYY94 pKa = 11.87GDD96 pKa = 4.87DD97 pKa = 4.41SGHH100 pKa = 5.43QPNRR104 pKa = 11.84AAVPFADD111 pKa = 5.43DD112 pKa = 3.96EE113 pKa = 4.67YY114 pKa = 11.62GDD116 pKa = 3.72FKK118 pKa = 10.78RR119 pKa = 11.84ARR121 pKa = 11.84EE122 pKa = 3.83RR123 pKa = 11.84LPQLFHH129 pKa = 6.81EE130 pKa = 5.31GWGFGSQQANEE141 pKa = 4.15EE142 pKa = 4.29SSEE145 pKa = 3.95GLQQQSGEE153 pKa = 4.31CGSGGGSSNAGGKK166 pKa = 10.01DD167 pKa = 3.35EE168 pKa = 6.87DD169 pKa = 4.63DD170 pKa = 4.02LQEE173 pKa = 4.18KK174 pKa = 8.85TGHH177 pKa = 5.66EE178 pKa = 4.22KK179 pKa = 10.33EE180 pKa = 4.39KK181 pKa = 11.16SVIVPPKK188 pKa = 9.66EE189 pKa = 4.02SGSVSGYY196 pKa = 10.28HH197 pKa = 6.53GSCDD201 pKa = 2.8IPFNMEE207 pKa = 3.92EE208 pKa = 4.35FLASPATTQRR218 pKa = 11.84RR219 pKa = 11.84QLLEE223 pKa = 4.25LCQLIADD230 pKa = 4.81RR231 pKa = 11.84SNEE234 pKa = 3.78EE235 pKa = 5.01LIFFPWGFNLVKK247 pKa = 10.44RR248 pKa = 11.84KK249 pKa = 6.48VHH251 pKa = 5.86RR252 pKa = 11.84VEE254 pKa = 3.96PQQPVAVSHH263 pKa = 6.62RR264 pKa = 11.84FTWEE268 pKa = 3.71DD269 pKa = 3.28FQLKK273 pKa = 9.91LKK275 pKa = 10.96AGFTLIHH282 pKa = 7.01KK283 pKa = 7.47KK284 pKa = 8.61TKK286 pKa = 10.68APVVLNSSSYY296 pKa = 10.63NLGSVPEE303 pKa = 4.38GGISLSPDD311 pKa = 3.22DD312 pKa = 4.6TEE314 pKa = 4.75LSVLIKK320 pKa = 10.16CLRR323 pKa = 11.84YY324 pKa = 9.51LGLYY328 pKa = 9.71KK329 pKa = 10.48FLATQIEE336 pKa = 4.55FF337 pKa = 3.68

Molecular weight: 37.29 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q4VKV7|PHOSP_TUPVT Phosphoprotein OS=Tupaia virus (isolate Tupaia/Thailand/-/1986) OX=1560034 GN=P PE=3 SV=1
MM1 pKa = 7.24TSQRR5 pKa = 11.84FQEE8 pKa = 3.98LLKK11 pKa = 10.83NVKK14 pKa = 9.15TNLGSVWVEE23 pKa = 3.55FRR25 pKa = 11.84SSVEE29 pKa = 3.63EE30 pKa = 4.1RR31 pKa = 11.84LKK33 pKa = 10.97HH34 pKa = 4.12PHH36 pKa = 4.87IWKK39 pKa = 10.11IIDD42 pKa = 3.49RR43 pKa = 11.84EE44 pKa = 4.11QLATILMFFLGIPKK58 pKa = 9.3MFRR61 pKa = 11.84LSQNMKK67 pKa = 8.95IRR69 pKa = 11.84QMRR72 pKa = 11.84NMRR75 pKa = 11.84KK76 pKa = 8.7PGVMEE81 pKa = 3.8MTPDD85 pKa = 3.56TNQTEE90 pKa = 4.61QPCPSQMTNTEE101 pKa = 4.02TSKK104 pKa = 10.84EE105 pKa = 4.13PEE107 pKa = 4.25SVSLNSSTKK116 pKa = 10.98DD117 pKa = 3.21GDD119 pKa = 3.98LDD121 pKa = 4.24PNKK124 pKa = 10.17QMKK127 pKa = 9.59NLRR130 pKa = 11.84RR131 pKa = 11.84DD132 pKa = 3.23SSSSQEE138 pKa = 4.22SADD141 pKa = 3.69LEE143 pKa = 4.28EE144 pKa = 4.42DD145 pKa = 3.01HH146 pKa = 7.2RR147 pKa = 11.84MLGGRR152 pKa = 11.84MRR154 pKa = 11.84MIFKK158 pKa = 10.22RR159 pKa = 11.84RR160 pKa = 11.84QGMRR164 pKa = 11.84KK165 pKa = 9.13KK166 pKa = 10.2SQSLSHH172 pKa = 6.22LRR174 pKa = 11.84SQAVYY179 pKa = 10.8QDD181 pKa = 3.5TTEE184 pKa = 4.05AAISPSTWKK193 pKa = 10.87SFWPPQLRR201 pKa = 11.84LRR203 pKa = 11.84DD204 pKa = 4.01DD205 pKa = 4.25NSWSSVNLSQTDD217 pKa = 3.53PTKK220 pKa = 11.15NN221 pKa = 3.09

Molecular weight: 25.78 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

3919

93

2107

559.9

63.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.44 ± 0.494

1.684 ± 0.265

5.307 ± 0.782

6.098 ± 0.709

5.078 ± 0.663

6.047 ± 0.592

2.194 ± 0.197

6.073 ± 0.617

6.022 ± 0.398

10.794 ± 1.157

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.067 ± 0.395

4.389 ± 0.21

4.746 ± 0.125

3.521 ± 0.57

5.792 ± 0.742

8.829 ± 0.373

5.639 ± 0.753

6.073 ± 0.545

1.99 ± 0.219

3.215 ± 0.316
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski