Meira miltonrushii

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina; Exobasidiomycetes; Exobasidiales; Brachybasidiaceae; Meira

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7452 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A316VCV2|A0A316VCV2_9BASI Uncharacterized protein OS=Meira miltonrushii OX=1280837 GN=FA14DRAFT_39994 PE=4 SV=1
MM1 pKa = 7.76PRR3 pKa = 11.84PNYY6 pKa = 9.2YY7 pKa = 10.67DD8 pKa = 3.62EE9 pKa = 5.68VEE11 pKa = 4.51LEE13 pKa = 4.05DD14 pKa = 3.37MSYY17 pKa = 11.34DD18 pKa = 3.58EE19 pKa = 5.25EE20 pKa = 5.8RR21 pKa = 11.84DD22 pKa = 3.61LFHH25 pKa = 6.84YY26 pKa = 7.92PCPCGDD32 pKa = 3.22RR33 pKa = 11.84FEE35 pKa = 4.25ITRR38 pKa = 11.84IQLKK42 pKa = 10.15DD43 pKa = 3.35AEE45 pKa = 4.53DD46 pKa = 3.6VARR49 pKa = 11.84CPSCSLIIRR58 pKa = 11.84VVFDD62 pKa = 4.08PLDD65 pKa = 3.73FEE67 pKa = 5.42EE68 pKa = 6.43DD69 pKa = 3.63DD70 pKa = 4.45DD71 pKa = 4.67VAPASQIATSATPVAQAAA89 pKa = 3.66

Molecular weight: 10.12 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A316V1W1|A0A316V1W1_9BASI Zn(2)-C6 fungal-type domain-containing protein OS=Meira miltonrushii OX=1280837 GN=FA14DRAFT_88449 PE=4 SV=1
MM1 pKa = 7.14SARR4 pKa = 11.84RR5 pKa = 11.84KK6 pKa = 8.02ISQYY10 pKa = 10.57ISCTTLNLFISNCSMKK26 pKa = 10.39NLRR29 pKa = 11.84PRR31 pKa = 11.84KK32 pKa = 9.29CIPNRR37 pKa = 11.84LHH39 pKa = 6.83PPFARR44 pKa = 11.84HH45 pKa = 5.97ALVACFCKK53 pKa = 10.84DD54 pKa = 3.27NVSVTRR60 pKa = 11.84LILPRR65 pKa = 11.84EE66 pKa = 4.24AMLDD70 pKa = 3.37LYY72 pKa = 11.4VRR74 pKa = 11.84LDD76 pKa = 3.65AKK78 pKa = 10.55CGKK81 pKa = 9.44LRR83 pKa = 11.84NSRR86 pKa = 3.61

Molecular weight: 9.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7452

0

7452

3681814

50

5274

494.1

54.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.638 ± 0.027

1.039 ± 0.01

5.639 ± 0.022

6.257 ± 0.026

3.545 ± 0.018

6.781 ± 0.031

2.355 ± 0.012

5.034 ± 0.02

5.498 ± 0.026

8.61 ± 0.032

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.231 ± 0.01

4.194 ± 0.017

5.321 ± 0.024

4.636 ± 0.02

6.035 ± 0.028

9.178 ± 0.04

5.742 ± 0.019

5.545 ± 0.02

1.218 ± 0.009

2.505 ± 0.015
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski