CrAssphage ZA

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; unclassified Podoviridae; crAss-like viruses

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 82 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A654JCH0|A0A654JCH0_9CAUD Uncharacterized protein OS=CrAssphage ZA OX=2507125 PE=4 SV=1
MM1 pKa = 7.77ALVLVNNSTINIISIIMTINTSHH24 pKa = 6.78ANIAGGITNDD34 pKa = 3.48ILNILDD40 pKa = 3.91ITDD43 pKa = 3.65SLYY46 pKa = 10.97TNDD49 pKa = 4.12TLDD52 pKa = 4.21TIDD55 pKa = 3.38IYY57 pKa = 8.62YY58 pKa = 9.82TRR60 pKa = 11.84GKK62 pKa = 9.77LIPIAIMILVILII75 pKa = 4.01

Molecular weight: 8.2 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A654JCJ8|A0A654JCJ8_9CAUD Putative integration host factor IHF subunit histone-like protein OS=CrAssphage ZA OX=2507125 PE=4 SV=1
MM1 pKa = 7.45MIQYY5 pKa = 10.18RR6 pKa = 11.84DD7 pKa = 3.44RR8 pKa = 11.84QRR10 pKa = 11.84QKK12 pKa = 11.26AFIGAIIGAAASIAGGIIKK31 pKa = 10.41GNKK34 pKa = 6.93QKK36 pKa = 10.76KK37 pKa = 8.6AQEE40 pKa = 4.06KK41 pKa = 9.7AQAEE45 pKa = 4.06AQAAQDD51 pKa = 4.57HH52 pKa = 6.93KK53 pKa = 11.39DD54 pKa = 3.36ALQNAQALTSAYY66 pKa = 10.47ANQDD70 pKa = 3.06YY71 pKa = 10.8VGQYY75 pKa = 9.84NDD77 pKa = 4.89KK78 pKa = 9.86LTLKK82 pKa = 10.6CGGRR86 pKa = 11.84VRR88 pKa = 11.84RR89 pKa = 11.84KK90 pKa = 10.2AGFGTEE96 pKa = 4.34FAQALPGLGNLASSITGVQGLGEE119 pKa = 5.0LGTAIGQGISANQQINEE136 pKa = 4.26NKK138 pKa = 10.27RR139 pKa = 11.84IAQEE143 pKa = 3.87AEE145 pKa = 3.67QRR147 pKa = 11.84KK148 pKa = 8.36QLQAGQQQLNITSDD162 pKa = 2.95KK163 pKa = 8.14MTNPMTMYY171 pKa = 10.1QRR173 pKa = 11.84SSFINKK179 pKa = 7.53YY180 pKa = 9.45KK181 pKa = 10.71CGGRR185 pKa = 11.84RR186 pKa = 11.84KK187 pKa = 9.61AWIGAAIGAAGSLIGGMFGSKK208 pKa = 9.45GQQPIQVKK216 pKa = 9.63QADD219 pKa = 3.89QASYY223 pKa = 10.54SAPKK227 pKa = 9.52TGLEE231 pKa = 3.85RR232 pKa = 11.84PEE234 pKa = 4.53WITNGNVQPSVMPQSVYY251 pKa = 10.4RR252 pKa = 11.84DD253 pKa = 3.32RR254 pKa = 11.84LNVYY258 pKa = 9.54RR259 pKa = 11.84CGGLRR264 pKa = 11.84RR265 pKa = 3.81

Molecular weight: 28.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

82

0

82

30546

66

4091

372.5

42.36

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.844 ± 0.301

1.054 ± 0.153

6.731 ± 0.217

6.142 ± 0.245

3.86 ± 0.178

6.06 ± 0.282

1.388 ± 0.111

8.803 ± 0.262

7.792 ± 0.272

7.019 ± 0.223

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.013 ± 0.139

8.836 ± 0.33

2.753 ± 0.115

3.126 ± 0.198

4.112 ± 0.154

5.994 ± 0.379

6.119 ± 0.407

5.975 ± 0.197

1.113 ± 0.097

5.267 ± 0.211
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski