Tomato mottle Taino virus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Geminiviridae; Begomovirus

Average proteome isoelectric point is 8.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|O36625|O36625_9GEMI Nuclear shuttle protein OS=Tomato mottle Taino virus OX=223358 GN=BR1 PE=3 SV=1
MM1 pKa = 7.31PRR3 pKa = 11.84KK4 pKa = 10.12GSFSVKK10 pKa = 9.71AKK12 pKa = 10.71NYY14 pKa = 9.36FLTYY18 pKa = 8.42PQCSLSKK25 pKa = 10.96EE26 pKa = 4.08EE27 pKa = 5.25ALSQIQNLNTPVDD40 pKa = 3.48KK41 pKa = 11.06KK42 pKa = 10.74FIKK45 pKa = 9.77ICRR48 pKa = 11.84EE49 pKa = 3.52LHH51 pKa = 6.26EE52 pKa = 4.93NGEE55 pKa = 4.29PHH57 pKa = 6.86LHH59 pKa = 5.98VLVQFEE65 pKa = 4.42GKK67 pKa = 10.12YY68 pKa = 10.03NCTNNRR74 pKa = 11.84FFDD77 pKa = 3.92VVSPTRR83 pKa = 11.84SAHH86 pKa = 4.86FHH88 pKa = 6.55PNIQGAKK95 pKa = 9.34SSSDD99 pKa = 2.95VKK101 pKa = 10.49TYY103 pKa = 8.38ITKK106 pKa = 10.84DD107 pKa = 2.99GDD109 pKa = 3.64TLEE112 pKa = 4.12WGEE115 pKa = 3.84FQIDD119 pKa = 3.21GRR121 pKa = 11.84SARR124 pKa = 11.84GGQQSANDD132 pKa = 3.98SYY134 pKa = 12.05AKK136 pKa = 10.5ALNAGNVQSAMAVLKK151 pKa = 10.56EE152 pKa = 4.02EE153 pKa = 4.08QPKK156 pKa = 11.09DD157 pKa = 3.79FVLHH161 pKa = 5.07NHH163 pKa = 6.85NIRR166 pKa = 11.84SNLEE170 pKa = 3.88RR171 pKa = 11.84IFAKK175 pKa = 10.49APEE178 pKa = 4.27PWVPPFPLSSFTNVPDD194 pKa = 5.1EE195 pKa = 4.13MQEE198 pKa = 3.77WADD201 pKa = 3.59VYY203 pKa = 10.95FGAGSAARR211 pKa = 11.84PNRR214 pKa = 11.84PMSIVIEE221 pKa = 4.37GDD223 pKa = 3.13SRR225 pKa = 11.84TGKK228 pKa = 8.55TMWARR233 pKa = 11.84ALGPHH238 pKa = 6.63NYY240 pKa = 10.14LSGHH244 pKa = 6.81LDD246 pKa = 3.29FNTRR250 pKa = 11.84VYY252 pKa = 11.35SNDD255 pKa = 2.71VQYY258 pKa = 11.73NVIDD262 pKa = 5.36DD263 pKa = 4.14IAPHH267 pKa = 5.05YY268 pKa = 10.73LKK270 pKa = 10.71LKK272 pKa = 9.14HH273 pKa = 5.83WKK275 pKa = 9.4EE276 pKa = 3.64LLGAQRR282 pKa = 11.84DD283 pKa = 3.78WQSNCKK289 pKa = 9.41YY290 pKa = 10.21GKK292 pKa = 9.18PVQIKK297 pKa = 9.93GGIPAIVLCNPGEE310 pKa = 4.16GASYY314 pKa = 11.33KK315 pKa = 10.88DD316 pKa = 3.79FLSKK320 pKa = 11.17DD321 pKa = 3.61EE322 pKa = 4.15NTSLRR327 pKa = 11.84DD328 pKa = 3.42WTIKK332 pKa = 10.41NAMFITLTTPLYY344 pKa = 10.33QDD346 pKa = 3.09STQARR351 pKa = 11.84QEE353 pKa = 4.07EE354 pKa = 5.14GNQEE358 pKa = 4.17TACC361 pKa = 4.22

Molecular weight: 40.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|O36622|O36622_9GEMI Replication enhancer OS=Tomato mottle Taino virus OX=223358 PE=3 SV=1
MM1 pKa = 7.92PKK3 pKa = 9.36RR4 pKa = 11.84DD5 pKa = 3.71RR6 pKa = 11.84TWRR9 pKa = 11.84SIAGTSKK16 pKa = 10.26VSRR19 pKa = 11.84NANYY23 pKa = 10.37SPRR26 pKa = 11.84TGSGPIGNKK35 pKa = 9.26ASEE38 pKa = 4.22WVNRR42 pKa = 11.84PMYY45 pKa = 9.81RR46 pKa = 11.84KK47 pKa = 8.87PKK49 pKa = 8.54IYY51 pKa = 10.76RR52 pKa = 11.84MMRR55 pKa = 11.84TPDD58 pKa = 3.26VPRR61 pKa = 11.84GCEE64 pKa = 4.34GPCKK68 pKa = 9.32VQSYY72 pKa = 6.94EE73 pKa = 3.74QRR75 pKa = 11.84HH76 pKa = 6.38DD77 pKa = 3.16ISHH80 pKa = 6.11VGKK83 pKa = 10.43VMCVSDD89 pKa = 3.48VTRR92 pKa = 11.84GNGITHH98 pKa = 6.78RR99 pKa = 11.84VGKK102 pKa = 9.44RR103 pKa = 11.84FCVKK107 pKa = 9.92SVYY110 pKa = 10.16ILGKK114 pKa = 9.37IWMDD118 pKa = 3.39EE119 pKa = 4.0NIKK122 pKa = 10.61LKK124 pKa = 10.73NHH126 pKa = 5.95TNSVMFWLVRR136 pKa = 11.84DD137 pKa = 3.7RR138 pKa = 11.84RR139 pKa = 11.84PYY141 pKa = 8.05GTPMDD146 pKa = 4.6FGQVFNMYY154 pKa = 10.66DD155 pKa = 3.86NEE157 pKa = 4.34PSTATVKK164 pKa = 10.81NDD166 pKa = 2.84LRR168 pKa = 11.84DD169 pKa = 3.6RR170 pKa = 11.84YY171 pKa = 9.91QVMHH175 pKa = 7.06RR176 pKa = 11.84FHH178 pKa = 6.63TKK180 pKa = 7.99VTGGQYY186 pKa = 11.04ASNEE190 pKa = 3.84QALVRR195 pKa = 11.84RR196 pKa = 11.84FWKK199 pKa = 10.4VNNHH203 pKa = 4.48VVYY206 pKa = 10.66NHH208 pKa = 5.84QEE210 pKa = 3.45AAKK213 pKa = 10.4YY214 pKa = 9.25EE215 pKa = 4.13NHH217 pKa = 6.46TEE219 pKa = 3.99NALLLYY225 pKa = 7.29MACTHH230 pKa = 7.07ASNPVYY236 pKa = 9.86ATLKK240 pKa = 9.47IRR242 pKa = 11.84IYY244 pKa = 10.6FYY246 pKa = 11.36DD247 pKa = 4.19SILNN251 pKa = 3.75

Molecular weight: 29.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

1424

129

361

237.3

27.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.618 ± 0.612

1.966 ± 0.293

5.126 ± 0.455

4.494 ± 0.558

4.354 ± 0.464

5.688 ± 0.589

3.792 ± 0.248

5.478 ± 0.427

6.18 ± 0.483

6.461 ± 0.524

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.879 ± 0.571

5.758 ± 0.781

4.775 ± 0.657

4.073 ± 0.665

7.163 ± 0.842

8.357 ± 0.918

5.548 ± 0.279

6.601 ± 0.59

1.545 ± 0.227

4.143 ± 0.616
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski