Blumeria graminis f. sp. hordei (Barley powdery mildew) (Oidium monilioides f. sp. hordei)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Erysiphales; Erysiphaceae; Blumeria

Average proteome isoelectric point is 6.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5276 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A383UHZ5|A0A383UHZ5_BLUGH Uncharacterized protein OS=Blumeria graminis f. sp. hordei OX=62688 GN=BLGHR1_10633 PE=4 SV=1
MM1 pKa = 7.85WEE3 pKa = 4.52LQAQCPPTHH12 pKa = 7.09PSYY15 pKa = 11.05HH16 pKa = 6.89PNQDD20 pKa = 2.82FDD22 pKa = 4.28NPGPSQISYY31 pKa = 10.6DD32 pKa = 3.67YY33 pKa = 11.2GITLVYY39 pKa = 10.4HH40 pKa = 6.7EE41 pKa = 5.76LPDD44 pKa = 4.29EE45 pKa = 4.5PTDD48 pKa = 4.94DD49 pKa = 5.14PFDD52 pKa = 4.03APNPSGSFRR61 pKa = 11.84EE62 pKa = 3.97ALGRR66 pKa = 11.84SVEE69 pKa = 4.04AAEE72 pKa = 4.29IQAKK76 pKa = 8.59EE77 pKa = 4.05AEE79 pKa = 4.26

Molecular weight: 8.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A383V0J2|A0A383V0J2_BLUGH Uncharacterized protein OS=Blumeria graminis f. sp. hordei OX=62688 GN=BLGHR1_16329 PE=4 SV=1
MM1 pKa = 7.39NISHH5 pKa = 8.12RR6 pKa = 11.84IRR8 pKa = 11.84SALTMTSLHH17 pKa = 6.48SPSSSTSSEE26 pKa = 3.76ADD28 pKa = 3.21DD29 pKa = 5.02SNLHH33 pKa = 6.38SPPTTPNPGRR43 pKa = 11.84GRR45 pKa = 11.84TLTSASSCSTSSIISLPALRR65 pKa = 11.84LKK67 pKa = 9.84KK68 pKa = 9.26TFTNLRR74 pKa = 11.84GAKK77 pKa = 8.1QRR79 pKa = 11.84RR80 pKa = 11.84KK81 pKa = 10.01DD82 pKa = 3.38EE83 pKa = 4.29RR84 pKa = 11.84YY85 pKa = 9.65RR86 pKa = 11.84EE87 pKa = 4.3ALAEE91 pKa = 3.82WAEE94 pKa = 3.78ADD96 pKa = 3.56EE97 pKa = 5.97RR98 pKa = 11.84EE99 pKa = 4.26WQYY102 pKa = 8.98PTWGGPAKK110 pKa = 10.38KK111 pKa = 9.68SRR113 pKa = 11.84KK114 pKa = 8.75FSRR117 pKa = 11.84EE118 pKa = 3.34QRR120 pKa = 11.84DD121 pKa = 3.77LLRR124 pKa = 11.84SWDD127 pKa = 3.46WHH129 pKa = 5.15QSTPRR134 pKa = 11.84TSLEE138 pKa = 3.43LRR140 pKa = 11.84TRR142 pKa = 11.84RR143 pKa = 11.84SSIFDD148 pKa = 4.26DD149 pKa = 4.73NISPCTSRR157 pKa = 11.84QNSLTGRR164 pKa = 11.84SSSLQGVYY172 pKa = 7.28TTAGRR177 pKa = 11.84HH178 pKa = 4.46QSVSTASSRR187 pKa = 11.84DD188 pKa = 3.32RR189 pKa = 11.84RR190 pKa = 11.84PCTVCGNEE198 pKa = 3.7

Molecular weight: 22.24 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5276

0

5276

2485222

21

4885

471.0

52.76

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.842 ± 0.028

1.375 ± 0.012

5.419 ± 0.025

6.489 ± 0.038

3.788 ± 0.022

5.694 ± 0.029

2.361 ± 0.013

6.143 ± 0.02

5.993 ± 0.031

9.074 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.133 ± 0.012

4.776 ± 0.02

5.318 ± 0.03

4.123 ± 0.021

5.821 ± 0.024

9.245 ± 0.043

5.92 ± 0.019

5.428 ± 0.023

1.222 ± 0.012

2.837 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski