Amino acid dipepetide frequency for LeviOr01 phage

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.811AlaAla: 8.811 ± 2.243
1.762AlaCys: 1.762 ± 1.218
3.524AlaAsp: 3.524 ± 2.981
6.167AlaGlu: 6.167 ± 0.469
1.762AlaPhe: 1.762 ± 1.218
8.811AlaGly: 8.811 ± 1.529
0.881AlaHis: 0.881 ± 0.609
6.167AlaIle: 6.167 ± 2.994
3.524AlaLys: 3.524 ± 1.318
7.93AlaLeu: 7.93 ± 2.08
2.643AlaMet: 2.643 ± 0.936
0.881AlaAsn: 0.881 ± 0.687
4.405AlaPro: 4.405 ± 2.271
3.524AlaGln: 3.524 ± 1.318
7.93AlaArg: 7.93 ± 1.733
6.167AlaSer: 6.167 ± 2.045
5.286AlaThr: 5.286 ± 3.104
4.405AlaVal: 4.405 ± 1.909
0.881AlaTrp: 0.881 ± 0.687
3.524AlaTyr: 3.524 ± 1.318
0.0AlaXaa: 0.0 ± 0.0
Cys
0.0CysAla: 0.0 ± 0.0
0.0CysCys: 0.0 ± 0.0
0.881CysAsp: 0.881 ± 0.609
0.881CysGlu: 0.881 ± 0.687
0.0CysPhe: 0.0 ± 0.0
1.762CysGly: 1.762 ± 1.218
0.0CysHis: 0.0 ± 0.0
2.643CysIle: 2.643 ± 0.757
0.0CysLys: 0.0 ± 0.0
0.881CysLeu: 0.881 ± 0.609
0.0CysMet: 0.0 ± 0.0
0.0CysAsn: 0.0 ± 0.0
0.0CysPro: 0.0 ± 0.0
0.0CysGln: 0.0 ± 0.0
1.762CysArg: 1.762 ± 1.218
0.881CysSer: 0.881 ± 0.609
1.762CysThr: 1.762 ± 1.218
0.881CysVal: 0.881 ± 0.609
0.881CysTrp: 0.881 ± 0.687
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
4.405AspAla: 4.405 ± 2.271
2.643AspCys: 2.643 ± 1.827
3.524AspAsp: 3.524 ± 2.436
3.524AspGlu: 3.524 ± 1.318
1.762AspPhe: 1.762 ± 0.39
2.643AspGly: 2.643 ± 1.779
0.0AspHis: 0.0 ± 0.0
6.167AspIle: 6.167 ± 0.986
0.0AspLys: 0.0 ± 0.0
2.643AspLeu: 2.643 ± 1.119
0.881AspMet: 0.881 ± 0.687
0.0AspAsn: 0.0 ± 0.0
2.643AspPro: 2.643 ± 1.779
2.643AspGln: 2.643 ± 0.757
6.167AspArg: 6.167 ± 1.374
3.524AspSer: 3.524 ± 1.012
3.524AspThr: 3.524 ± 1.012
7.048AspVal: 7.048 ± 1.558
1.762AspTrp: 1.762 ± 1.218
1.762AspTyr: 1.762 ± 0.39
0.0AspXaa: 0.0 ± 0.0
Glu
2.643GluAla: 2.643 ± 1.827
0.881GluCys: 0.881 ± 0.609
2.643GluAsp: 2.643 ± 1.827
2.643GluGlu: 2.643 ± 0.757
5.286GluPhe: 5.286 ± 1.169
0.881GluGly: 0.881 ± 0.687
0.881GluHis: 0.881 ± 0.687
1.762GluIle: 1.762 ± 1.374
4.405GluLys: 4.405 ± 2.888
6.167GluLeu: 6.167 ± 1.611
0.0GluMet: 0.0 ± 0.0
1.762GluAsn: 1.762 ± 0.39
6.167GluPro: 6.167 ± 2.062
0.881GluGln: 0.881 ± 0.687
6.167GluArg: 6.167 ± 1.611
4.405GluSer: 4.405 ± 1.241
2.643GluThr: 2.643 ± 2.061
6.167GluVal: 6.167 ± 1.592
2.643GluTrp: 2.643 ± 0.936
2.643GluTyr: 2.643 ± 1.827
0.0GluXaa: 0.0 ± 0.0
Phe
2.643PheAla: 2.643 ± 1.827
0.0PheCys: 0.0 ± 0.0
2.643PheAsp: 2.643 ± 0.936
4.405PheGlu: 4.405 ± 1.259
0.0PhePhe: 0.0 ± 0.0
0.881PheGly: 0.881 ± 0.687
0.0PheHis: 0.0 ± 0.0
2.643PheIle: 2.643 ± 0.936
1.762PheLys: 1.762 ± 0.39
1.762PheLeu: 1.762 ± 0.39
1.762PheMet: 1.762 ± 1.218
0.881PheAsn: 0.881 ± 0.687
2.643PhePro: 2.643 ± 1.827
0.0PheGln: 0.0 ± 0.0
5.286PheArg: 5.286 ± 1.515
3.524PheSer: 3.524 ± 1.318
1.762PheThr: 1.762 ± 1.395
2.643PheVal: 2.643 ± 0.757
0.0PheTrp: 0.0 ± 0.0
0.881PheTyr: 0.881 ± 0.609
0.0PheXaa: 0.0 ± 0.0
Gly
2.643GlyAla: 2.643 ± 1.119
0.881GlyCys: 0.881 ± 0.609
6.167GlyAsp: 6.167 ± 0.986
0.881GlyGlu: 0.881 ± 0.609
2.643GlyPhe: 2.643 ± 0.936
3.524GlyGly: 3.524 ± 1.012
0.0GlyHis: 0.0 ± 0.0
3.524GlyIle: 3.524 ± 0.779
2.643GlyLys: 2.643 ± 0.936
7.048GlyLeu: 7.048 ± 1.51
1.762GlyMet: 1.762 ± 1.374
3.524GlyAsn: 3.524 ± 1.596
4.405GlyPro: 4.405 ± 0.764
0.0GlyGln: 0.0 ± 0.0
5.286GlyArg: 5.286 ± 0.638
4.405GlySer: 4.405 ± 1.259
2.643GlyThr: 2.643 ± 4.474
2.643GlyVal: 2.643 ± 2.061
2.643GlyTrp: 2.643 ± 2.061
3.524GlyTyr: 3.524 ± 1.318
0.0GlyXaa: 0.0 ± 0.0
His
0.881HisAla: 0.881 ± 0.609
0.0HisCys: 0.0 ± 0.0
0.881HisAsp: 0.881 ± 1.491
0.881HisGlu: 0.881 ± 0.687
0.0HisPhe: 0.0 ± 0.0
2.643HisGly: 2.643 ± 0.757
0.881HisHis: 0.881 ± 0.609
0.0HisIle: 0.0 ± 0.0
0.0HisLys: 0.0 ± 0.0
1.762HisLeu: 1.762 ± 0.39
0.0HisMet: 0.0 ± 0.0
0.0HisAsn: 0.0 ± 0.0
0.0HisPro: 0.0 ± 0.0
0.0HisGln: 0.0 ± 0.0
0.0HisArg: 0.0 ± 0.0
0.0HisSer: 0.0 ± 0.0
1.762HisThr: 1.762 ± 1.218
2.643HisVal: 2.643 ± 2.061
1.762HisTrp: 1.762 ± 0.39
0.0HisTyr: 0.0 ± 0.0
0.0HisXaa: 0.0 ± 0.0
Ile
6.167IleAla: 6.167 ± 2.524
0.0IleCys: 0.0 ± 0.0
2.643IleAsp: 2.643 ± 0.936
0.0IleGlu: 0.0 ± 0.0
3.524IlePhe: 3.524 ± 1.318
1.762IleGly: 1.762 ± 1.374
0.0IleHis: 0.0 ± 0.0
0.881IleIle: 0.881 ± 0.609
2.643IleLys: 2.643 ± 0.936
0.881IleLeu: 0.881 ± 0.609
0.0IleMet: 0.0 ± 1.079
2.643IleAsn: 2.643 ± 1.552
4.405IlePro: 4.405 ± 1.039
1.762IleGln: 1.762 ± 1.374
5.286IleArg: 5.286 ± 0.638
4.405IleSer: 4.405 ± 3.21
2.643IleThr: 2.643 ± 1.779
4.405IleVal: 4.405 ± 1.241
0.881IleTrp: 0.881 ± 0.609
0.881IleTyr: 0.881 ± 0.687
0.0IleXaa: 0.0 ± 0.0
Lys
4.405LysAla: 4.405 ± 1.643
1.762LysCys: 1.762 ± 1.218
0.881LysAsp: 0.881 ± 0.609
3.524LysGlu: 3.524 ± 1.318
0.0LysPhe: 0.0 ± 0.0
2.643LysGly: 2.643 ± 2.061
0.881LysHis: 0.881 ± 0.609
0.881LysIle: 0.881 ± 0.609
0.881LysLys: 0.881 ± 0.609
3.524LysLeu: 3.524 ± 1.012
0.881LysMet: 0.881 ± 0.687
0.0LysAsn: 0.0 ± 0.0
4.405LysPro: 4.405 ± 2.271
1.762LysGln: 1.762 ± 1.374
3.524LysArg: 3.524 ± 1.012
0.881LysSer: 0.881 ± 0.609
3.524LysThr: 3.524 ± 0.779
4.405LysVal: 4.405 ± 1.643
1.762LysTrp: 1.762 ± 0.39
0.881LysTyr: 0.881 ± 0.687
0.0LysXaa: 0.0 ± 0.0
Leu
10.573LeuAla: 10.573 ± 3.863
0.0LeuCys: 0.0 ± 0.0
5.286LeuAsp: 5.286 ± 0.638
7.048LeuGlu: 7.048 ± 1.558
3.524LeuPhe: 3.524 ± 1.318
4.405LeuGly: 4.405 ± 1.039
1.762LeuHis: 1.762 ± 0.39
4.405LeuIle: 4.405 ± 1.259
4.405LeuLys: 4.405 ± 0.764
6.167LeuLeu: 6.167 ± 1.374
0.881LeuMet: 0.881 ± 0.609
3.524LeuAsn: 3.524 ± 0.779
8.811LeuPro: 8.811 ± 1.081
5.286LeuGln: 5.286 ± 2.238
7.93LeuArg: 7.93 ± 0.842
6.167LeuSer: 6.167 ± 2.045
4.405LeuThr: 4.405 ± 2.434
6.167LeuVal: 6.167 ± 2.245
0.0LeuTrp: 0.0 ± 0.0
1.762LeuTyr: 1.762 ± 0.39
0.0LeuXaa: 0.0 ± 0.0
Met
2.643MetAla: 2.643 ± 0.936
0.0MetCys: 0.0 ± 0.0
2.643MetAsp: 2.643 ± 0.936
2.643MetGlu: 2.643 ± 1.827
0.0MetPhe: 0.0 ± 0.0
0.881MetGly: 0.881 ± 0.609
0.881MetHis: 0.881 ± 0.609
0.0MetIle: 0.0 ± 0.0
0.0MetLys: 0.0 ± 0.0
1.762MetLeu: 1.762 ± 0.39
0.0MetMet: 0.0 ± 0.0
0.0MetAsn: 0.0 ± 0.0
0.881MetPro: 0.881 ± 0.687
0.0MetGln: 0.0 ± 0.0
2.643MetArg: 2.643 ± 0.936
1.762MetSer: 1.762 ± 1.395
0.0MetThr: 0.0 ± 0.0
2.643MetVal: 2.643 ± 1.779
0.0MetTrp: 0.0 ± 0.0
0.881MetTyr: 0.881 ± 0.609
0.0MetXaa: 0.0 ± 0.0
Asn
3.524AsnAla: 3.524 ± 1.012
0.881AsnCys: 0.881 ± 0.609
0.881AsnAsp: 0.881 ± 0.609
1.762AsnGlu: 1.762 ± 1.374
1.762AsnPhe: 1.762 ± 0.39
4.405AsnGly: 4.405 ± 2.434
1.762AsnHis: 1.762 ± 0.39
1.762AsnIle: 1.762 ± 1.374
1.762AsnLys: 1.762 ± 1.374
4.405AsnLeu: 4.405 ± 1.259
2.643AsnMet: 2.643 ± 1.552
0.0AsnAsn: 0.0 ± 0.0
0.881AsnPro: 0.881 ± 0.687
1.762AsnGln: 1.762 ± 1.218
2.643AsnArg: 2.643 ± 0.936
0.881AsnSer: 0.881 ± 0.609
0.881AsnThr: 0.881 ± 0.687
0.0AsnVal: 0.0 ± 0.0
0.0AsnTrp: 0.0 ± 0.0
0.0AsnTyr: 0.0 ± 0.0
0.0AsnXaa: 0.0 ± 0.0
Pro
7.93ProAla: 7.93 ± 2.08
1.762ProCys: 1.762 ± 1.374
1.762ProAsp: 1.762 ± 0.39
5.286ProGlu: 5.286 ± 1.515
3.524ProPhe: 3.524 ± 1.318
1.762ProGly: 1.762 ± 0.39
0.0ProHis: 0.0 ± 0.0
0.881ProIle: 0.881 ± 0.687
7.048ProLys: 7.048 ± 0.988
8.811ProLeu: 8.811 ± 0.289
2.643ProMet: 2.643 ± 0.983
2.643ProAsn: 2.643 ± 1.552
5.286ProPro: 5.286 ± 1.873
0.0ProGln: 0.0 ± 0.0
3.524ProArg: 3.524 ± 1.318
1.762ProSer: 1.762 ± 1.218
2.643ProThr: 2.643 ± 2.061
4.405ProVal: 4.405 ± 1.039
0.881ProTrp: 0.881 ± 0.687
0.881ProTyr: 0.881 ± 0.687
0.0ProXaa: 0.0 ± 0.0
Gln
0.0GlnAla: 0.0 ± 0.0
0.0GlnCys: 0.0 ± 0.0
0.881GlnAsp: 0.881 ± 0.609
3.524GlnGlu: 3.524 ± 1.596
0.881GlnPhe: 0.881 ± 0.687
0.881GlnGly: 0.881 ± 0.609
0.0GlnHis: 0.0 ± 0.0
0.0GlnIle: 0.0 ± 0.0
1.762GlnLys: 1.762 ± 0.39
2.643GlnLeu: 2.643 ± 1.119
0.0GlnMet: 0.0 ± 0.0
0.881GlnAsn: 0.881 ± 0.687
0.0GlnPro: 0.0 ± 0.0
0.881GlnGln: 0.881 ± 0.687
3.524GlnArg: 3.524 ± 1.596
2.643GlnSer: 2.643 ± 1.552
0.881GlnThr: 0.881 ± 0.609
3.524GlnVal: 3.524 ± 2.608
0.0GlnTrp: 0.0 ± 0.0
0.881GlnTyr: 0.881 ± 1.491
0.0GlnXaa: 0.0 ± 0.0
Arg
6.167ArgAla: 6.167 ± 0.469
0.881ArgCys: 0.881 ± 0.609
9.692ArgAsp: 9.692 ± 2.352
7.048ArgGlu: 7.048 ± 2.636
2.643ArgPhe: 2.643 ± 0.757
3.524ArgGly: 3.524 ± 1.596
2.643ArgHis: 2.643 ± 0.757
0.881ArgIle: 0.881 ± 1.491
5.286ArgLys: 5.286 ± 1.873
5.286ArgLeu: 5.286 ± 1.672
2.643ArgMet: 2.643 ± 0.936
5.286ArgAsn: 5.286 ± 1.043
4.405ArgPro: 4.405 ± 2.358
0.881ArgGln: 0.881 ± 0.687
3.524ArgArg: 3.524 ± 1.596
6.167ArgSer: 6.167 ± 1.611
10.573ArgThr: 10.573 ± 2.755
6.167ArgVal: 6.167 ± 1.374
0.881ArgTrp: 0.881 ± 0.609
7.048ArgTyr: 7.048 ± 1.776
0.0ArgXaa: 0.0 ± 0.0
Ser
5.286SerAla: 5.286 ± 1.169
0.881SerCys: 0.881 ± 0.609
3.524SerAsp: 3.524 ± 1.012
3.524SerGlu: 3.524 ± 1.012
3.524SerPhe: 3.524 ± 0.779
6.167SerGly: 6.167 ± 2.062
1.762SerHis: 1.762 ± 1.491
4.405SerIle: 4.405 ± 0.764
1.762SerLys: 1.762 ± 1.374
9.692SerLeu: 9.692 ± 1.695
0.0SerMet: 0.0 ± 0.0
2.643SerAsn: 2.643 ± 1.119
2.643SerPro: 2.643 ± 0.757
0.881SerGln: 0.881 ± 1.491
9.692SerArg: 9.692 ± 3.526
3.524SerSer: 3.524 ± 4.294
2.643SerThr: 2.643 ± 0.757
4.405SerVal: 4.405 ± 4.099
0.0SerTrp: 0.0 ± 0.0
2.643SerTyr: 2.643 ± 0.757
0.0SerXaa: 0.0 ± 0.0
Thr
7.048ThrAla: 7.048 ± 5.257
0.881ThrCys: 0.881 ± 0.687
0.881ThrAsp: 0.881 ± 0.609
2.643ThrGlu: 2.643 ± 1.119
3.524ThrPhe: 3.524 ± 1.012
4.405ThrGly: 4.405 ± 0.764
0.0ThrHis: 0.0 ± 0.0
3.524ThrIle: 3.524 ± 1.226
0.881ThrLys: 0.881 ± 0.687
7.93ThrLeu: 7.93 ± 2.181
2.643ThrMet: 2.643 ± 1.827
1.762ThrAsn: 1.762 ± 1.374
3.524ThrPro: 3.524 ± 2.436
1.762ThrGln: 1.762 ± 0.39
5.286ThrArg: 5.286 ± 1.873
6.167ThrSer: 6.167 ± 2.045
1.762ThrThr: 1.762 ± 1.218
2.643ThrVal: 2.643 ± 1.779
0.0ThrTrp: 0.0 ± 0.0
0.881ThrTyr: 0.881 ± 0.687
0.0ThrXaa: 0.0 ± 0.0
Val
10.573ValAla: 10.573 ± 1.207
0.0ValCys: 0.0 ± 0.0
5.286ValAsp: 5.286 ± 1.169
3.524ValGlu: 3.524 ± 2.247
0.0ValPhe: 0.0 ± 0.0
5.286ValGly: 5.286 ± 2.702
0.881ValHis: 0.881 ± 0.687
3.524ValIle: 3.524 ± 1.012
1.762ValLys: 1.762 ± 1.218
8.811ValLeu: 8.811 ± 2.243
0.0ValMet: 0.0 ± 0.0
2.643ValAsn: 2.643 ± 0.757
3.524ValPro: 3.524 ± 0.779
1.762ValGln: 1.762 ± 2.983
7.048ValArg: 7.048 ± 0.306
7.048ValSer: 7.048 ± 2.22
7.048ValThr: 7.048 ± 2.184
5.286ValVal: 5.286 ± 2.238
1.762ValTrp: 1.762 ± 1.395
0.881ValTyr: 0.881 ± 0.609
0.0ValXaa: 0.0 ± 0.0
Trp
1.762TrpAla: 1.762 ± 0.39
0.0TrpCys: 0.0 ± 0.0
1.762TrpAsp: 1.762 ± 0.39
1.762TrpGlu: 1.762 ± 0.39
0.881TrpPhe: 0.881 ± 0.609
0.0TrpGly: 0.0 ± 0.0
0.881TrpHis: 0.881 ± 0.687
0.881TrpIle: 0.881 ± 0.609
0.881TrpLys: 0.881 ± 0.687
2.643TrpLeu: 2.643 ± 0.936
0.0TrpMet: 0.0 ± 0.0
0.0TrpAsn: 0.0 ± 0.0
0.881TrpPro: 0.881 ± 0.609
0.0TrpGln: 0.0 ± 0.0
0.881TrpArg: 0.881 ± 0.609
2.643TrpSer: 2.643 ± 1.119
0.881TrpThr: 0.881 ± 0.609
2.643TrpVal: 2.643 ± 0.936
0.0TrpTrp: 0.0 ± 0.0
0.881TrpTyr: 0.881 ± 0.687
0.0TrpXaa: 0.0 ± 0.0
Tyr
0.881TyrAla: 0.881 ± 0.609
0.0TyrCys: 0.0 ± 0.0
0.881TyrAsp: 0.881 ± 0.687
0.0TyrGlu: 0.0 ± 0.0
0.881TyrPhe: 0.881 ± 0.609
3.524TyrGly: 3.524 ± 0.779
0.0TyrHis: 0.0 ± 0.0
1.762TyrIle: 1.762 ± 0.39
0.0TyrLys: 0.0 ± 0.0
1.762TyrLeu: 1.762 ± 1.218
0.0TyrMet: 0.0 ± 0.0
3.524TyrAsn: 3.524 ± 1.318
3.524TyrPro: 3.524 ± 0.779
0.0TyrGln: 0.0 ± 0.0
3.524TyrArg: 3.524 ± 1.596
2.643TyrSer: 2.643 ± 0.757
0.881TyrThr: 0.881 ± 0.609
3.524TyrVal: 3.524 ± 1.012
3.524TyrTrp: 3.524 ± 1.318
0.881TyrTyr: 0.881 ± 0.609
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3 proteins (1136 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski