Alphaproteobacteria bacterium PA4

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; unclassified Alphaproteobacteria

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2726 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A257ELW5|A0A257ELW5_9PROT Glyoxalase OS=Alphaproteobacteria bacterium PA4 OX=2015572 GN=CFE37_08345 PE=4 SV=1
MM1 pKa = 7.99AIRR4 pKa = 11.84NASLALGLIVAAATAATPVLADD26 pKa = 3.45TLSNHH31 pKa = 5.0TSRR34 pKa = 11.84YY35 pKa = 7.88RR36 pKa = 11.84VGVGFGPGEE45 pKa = 4.21SLSGGDD51 pKa = 3.31LNYY54 pKa = 10.91RR55 pKa = 11.84KK56 pKa = 8.31FTQGAASAEE65 pKa = 4.11IQISINPVPAINAAASNSDD84 pKa = 3.01AGTDD88 pKa = 2.91IFAFGFLGYY97 pKa = 10.58NYY99 pKa = 10.79AVDD102 pKa = 4.43FTAASGLVINDD113 pKa = 3.53LNIVDD118 pKa = 3.96VTGLAFLDD126 pKa = 3.95STLGGSVYY134 pKa = 11.14ANAGDD139 pKa = 4.49GLVAWACVDD148 pKa = 3.51GAGDD152 pKa = 3.69CGTFNFAVTDD162 pKa = 3.72NANSYY167 pKa = 10.72IEE169 pKa = 4.08TDD171 pKa = 3.49QLDD174 pKa = 3.88GTSII178 pKa = 3.49

Molecular weight: 18.01 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A257EM15|A0A257EM15_9PROT Aspartate--tRNA(Asp/Asn) ligase OS=Alphaproteobacteria bacterium PA4 OX=2015572 GN=aspS PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.43LVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.38GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84NVIRR32 pKa = 11.84NRR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84KK40 pKa = 9.26KK41 pKa = 10.65LSAA44 pKa = 3.91

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2726

0

2726

893394

35

4671

327.7

34.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.977 ± 0.077

0.713 ± 0.014

5.856 ± 0.04

4.373 ± 0.051

3.454 ± 0.029

9.263 ± 0.082

1.909 ± 0.029

4.866 ± 0.034

2.634 ± 0.039

10.285 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.288 ± 0.027

2.549 ± 0.052

5.58 ± 0.048

2.958 ± 0.025

7.162 ± 0.057

4.816 ± 0.046

5.531 ± 0.072

7.254 ± 0.038

1.451 ± 0.024

2.082 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski