Amino acid dipepetide frequency for Bacillus phage Leo2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
2.658AlaAla: 2.658 ± 0.745
0.682AlaCys: 0.682 ± 0.297
3.34AlaAsp: 3.34 ± 0.488
3.612AlaGlu: 3.612 ± 0.486
3.203AlaPhe: 3.203 ± 0.376
4.294AlaGly: 4.294 ± 0.649
1.159AlaHis: 1.159 ± 0.291
5.453AlaIle: 5.453 ± 0.948
5.657AlaLys: 5.657 ± 0.595
5.521AlaLeu: 5.521 ± 0.704
1.84AlaMet: 1.84 ± 0.362
2.931AlaAsn: 2.931 ± 0.429
2.317AlaPro: 2.317 ± 0.408
1.977AlaGln: 1.977 ± 0.383
2.999AlaArg: 2.999 ± 0.622
3.34AlaSer: 3.34 ± 0.54
3.135AlaThr: 3.135 ± 0.545
3.749AlaVal: 3.749 ± 0.524
0.886AlaTrp: 0.886 ± 0.214
3.135AlaTyr: 3.135 ± 0.375
0.0AlaXaa: 0.0 ± 0.0
Cys
0.477CysAla: 0.477 ± 0.171
0.068CysCys: 0.068 ± 0.075
0.545CysAsp: 0.545 ± 0.19
0.409CysGlu: 0.409 ± 0.158
0.613CysPhe: 0.613 ± 0.203
0.818CysGly: 0.818 ± 0.216
0.409CysHis: 0.409 ± 0.159
0.545CysIle: 0.545 ± 0.164
1.091CysLys: 1.091 ± 0.284
0.613CysLeu: 0.613 ± 0.212
0.136CysMet: 0.136 ± 0.1
0.273CysAsn: 0.273 ± 0.182
0.273CysPro: 0.273 ± 0.149
0.477CysGln: 0.477 ± 0.169
0.341CysArg: 0.341 ± 0.151
0.613CysSer: 0.613 ± 0.222
0.682CysThr: 0.682 ± 0.206
0.204CysVal: 0.204 ± 0.12
0.068CysTrp: 0.068 ± 0.064
0.477CysTyr: 0.477 ± 0.196
0.0CysXaa: 0.0 ± 0.0
Asp
3.067AspAla: 3.067 ± 0.504
0.75AspCys: 0.75 ± 0.254
4.021AspAsp: 4.021 ± 0.481
4.226AspGlu: 4.226 ± 0.554
3.749AspPhe: 3.749 ± 0.487
4.43AspGly: 4.43 ± 0.538
1.704AspHis: 1.704 ± 0.333
4.43AspIle: 4.43 ± 0.625
4.907AspLys: 4.907 ± 0.652
5.248AspLeu: 5.248 ± 0.609
1.568AspMet: 1.568 ± 0.319
2.249AspAsn: 2.249 ± 0.379
3.067AspPro: 3.067 ± 0.414
2.113AspGln: 2.113 ± 0.317
3.34AspArg: 3.34 ± 0.399
2.931AspSer: 2.931 ± 0.412
3.135AspThr: 3.135 ± 0.411
3.953AspVal: 3.953 ± 0.338
0.75AspTrp: 0.75 ± 0.22
2.522AspTyr: 2.522 ± 0.328
0.0AspXaa: 0.0 ± 0.0
Glu
5.453GluAla: 5.453 ± 0.699
0.545GluCys: 0.545 ± 0.2
4.089GluAsp: 4.089 ± 0.64
8.383GluGlu: 8.383 ± 1.125
3.203GluPhe: 3.203 ± 0.519
5.248GluGly: 5.248 ± 0.534
1.636GluHis: 1.636 ± 0.284
4.635GluIle: 4.635 ± 0.474
5.384GluLys: 5.384 ± 0.631
7.702GluLeu: 7.702 ± 0.817
2.794GluMet: 2.794 ± 0.523
3.408GluAsn: 3.408 ± 0.41
2.045GluPro: 2.045 ± 0.386
3.885GluGln: 3.885 ± 0.545
3.34GluArg: 3.34 ± 0.509
3.817GluSer: 3.817 ± 0.517
4.226GluThr: 4.226 ± 0.485
5.384GluVal: 5.384 ± 0.671
0.818GluTrp: 0.818 ± 0.194
3.68GluTyr: 3.68 ± 0.55
0.0GluXaa: 0.0 ± 0.0
Phe
2.794PheAla: 2.794 ± 0.556
0.136PheCys: 0.136 ± 0.093
3.612PheAsp: 3.612 ± 0.472
3.612PheGlu: 3.612 ± 0.451
2.045PhePhe: 2.045 ± 0.41
3.067PheGly: 3.067 ± 0.331
0.886PheHis: 0.886 ± 0.261
2.794PheIle: 2.794 ± 0.457
2.863PheLys: 2.863 ± 0.459
3.34PheLeu: 3.34 ± 0.474
1.568PheMet: 1.568 ± 0.372
2.317PheAsn: 2.317 ± 0.339
1.499PhePro: 1.499 ± 0.348
1.499PheGln: 1.499 ± 0.31
1.772PheArg: 1.772 ± 0.302
2.794PheSer: 2.794 ± 0.424
3.749PheThr: 3.749 ± 0.489
2.794PheVal: 2.794 ± 0.378
0.341PheTrp: 0.341 ± 0.124
1.977PheTyr: 1.977 ± 0.401
0.0PheXaa: 0.0 ± 0.0
Gly
5.112GlyAla: 5.112 ± 0.662
0.75GlyCys: 0.75 ± 0.196
4.771GlyAsp: 4.771 ± 0.642
4.703GlyGlu: 4.703 ± 0.481
3.272GlyPhe: 3.272 ± 0.396
4.839GlyGly: 4.839 ± 0.642
0.954GlyHis: 0.954 ± 0.258
4.43GlyIle: 4.43 ± 0.574
5.384GlyLys: 5.384 ± 0.574
5.044GlyLeu: 5.044 ± 0.578
1.908GlyMet: 1.908 ± 0.315
3.476GlyAsn: 3.476 ± 0.968
0.136GlyPro: 0.136 ± 0.118
2.181GlyGln: 2.181 ± 0.434
3.408GlyArg: 3.408 ± 0.468
5.384GlySer: 5.384 ± 0.711
3.408GlyThr: 3.408 ± 0.537
5.316GlyVal: 5.316 ± 0.858
0.954GlyTrp: 0.954 ± 0.244
2.794GlyTyr: 2.794 ± 0.385
0.0GlyXaa: 0.0 ± 0.0
His
0.954HisAla: 0.954 ± 0.257
0.136HisCys: 0.136 ± 0.105
0.886HisAsp: 0.886 ± 0.267
1.431HisGlu: 1.431 ± 0.325
1.363HisPhe: 1.363 ± 0.317
2.113HisGly: 2.113 ± 0.376
0.682HisHis: 0.682 ± 0.176
1.499HisIle: 1.499 ± 0.277
1.363HisLys: 1.363 ± 0.277
1.431HisLeu: 1.431 ± 0.367
0.341HisMet: 0.341 ± 0.147
1.159HisAsn: 1.159 ± 0.242
0.954HisPro: 0.954 ± 0.21
0.886HisGln: 0.886 ± 0.246
1.091HisArg: 1.091 ± 0.289
1.295HisSer: 1.295 ± 0.285
1.022HisThr: 1.022 ± 0.227
0.886HisVal: 0.886 ± 0.255
0.341HisTrp: 0.341 ± 0.14
1.022HisTyr: 1.022 ± 0.304
0.0HisXaa: 0.0 ± 0.0
Ile
4.089IleAla: 4.089 ± 0.545
0.477IleCys: 0.477 ± 0.18
4.907IleAsp: 4.907 ± 0.675
4.975IleGlu: 4.975 ± 0.533
2.113IlePhe: 2.113 ± 0.289
4.362IleGly: 4.362 ± 0.604
1.636IleHis: 1.636 ± 0.279
3.885IleIle: 3.885 ± 0.462
6.543IleLys: 6.543 ± 0.721
4.771IleLeu: 4.771 ± 0.427
1.908IleMet: 1.908 ± 0.369
3.612IleAsn: 3.612 ± 0.511
2.658IlePro: 2.658 ± 0.377
2.658IleGln: 2.658 ± 0.404
2.999IleArg: 2.999 ± 0.41
3.544IleSer: 3.544 ± 0.476
4.294IleThr: 4.294 ± 0.482
4.567IleVal: 4.567 ± 0.574
0.409IleTrp: 0.409 ± 0.164
2.385IleTyr: 2.385 ± 0.49
0.0IleXaa: 0.0 ± 0.0
Lys
4.43LysAla: 4.43 ± 0.539
0.477LysCys: 0.477 ± 0.202
5.044LysAsp: 5.044 ± 0.653
8.52LysGlu: 8.52 ± 0.988
3.885LysPhe: 3.885 ± 0.55
4.771LysGly: 4.771 ± 0.843
2.249LysHis: 2.249 ± 0.387
3.953LysIle: 3.953 ± 0.568
7.293LysLys: 7.293 ± 0.803
5.384LysLeu: 5.384 ± 0.607
2.931LysMet: 2.931 ± 0.456
3.68LysAsn: 3.68 ± 0.425
3.067LysPro: 3.067 ± 0.463
3.408LysGln: 3.408 ± 0.44
4.158LysArg: 4.158 ± 0.624
4.294LysSer: 4.294 ± 0.586
3.749LysThr: 3.749 ± 0.609
5.384LysVal: 5.384 ± 0.643
0.613LysTrp: 0.613 ± 0.191
2.522LysTyr: 2.522 ± 0.433
0.0LysXaa: 0.0 ± 0.0
Leu
4.567LeuAla: 4.567 ± 0.584
0.613LeuCys: 0.613 ± 0.209
4.635LeuAsp: 4.635 ± 0.5
6.475LeuGlu: 6.475 ± 0.747
2.385LeuPhe: 2.385 ± 0.36
6.407LeuGly: 6.407 ± 0.659
1.499LeuHis: 1.499 ± 0.308
5.248LeuIle: 5.248 ± 0.591
6.952LeuLys: 6.952 ± 0.811
5.93LeuLeu: 5.93 ± 0.621
2.045LeuMet: 2.045 ± 0.437
4.021LeuAsn: 4.021 ± 0.416
2.999LeuPro: 2.999 ± 0.484
2.726LeuGln: 2.726 ± 0.469
3.067LeuArg: 3.067 ± 0.396
5.93LeuSer: 5.93 ± 0.685
4.839LeuThr: 4.839 ± 0.515
4.771LeuVal: 4.771 ± 0.519
0.682LeuTrp: 0.682 ± 0.188
2.794LeuTyr: 2.794 ± 0.514
0.0LeuXaa: 0.0 ± 0.0
Met
2.522MetAla: 2.522 ± 0.414
0.204MetCys: 0.204 ± 0.101
1.636MetAsp: 1.636 ± 0.272
1.636MetGlu: 1.636 ± 0.387
1.227MetPhe: 1.227 ± 0.299
1.499MetGly: 1.499 ± 0.318
0.545MetHis: 0.545 ± 0.19
1.295MetIle: 1.295 ± 0.288
2.726MetLys: 2.726 ± 0.397
1.568MetLeu: 1.568 ± 0.333
0.613MetMet: 0.613 ± 0.199
1.295MetAsn: 1.295 ± 0.215
0.613MetPro: 0.613 ± 0.212
0.613MetGln: 0.613 ± 0.202
1.022MetArg: 1.022 ± 0.252
3.34MetSer: 3.34 ± 0.515
2.045MetThr: 2.045 ± 0.327
1.159MetVal: 1.159 ± 0.249
0.273MetTrp: 0.273 ± 0.157
0.818MetTyr: 0.818 ± 0.283
0.0MetXaa: 0.0 ± 0.0
Asn
3.067AsnAla: 3.067 ± 0.403
0.682AsnCys: 0.682 ± 0.271
1.908AsnAsp: 1.908 ± 0.31
3.749AsnGlu: 3.749 ± 0.461
2.113AsnPhe: 2.113 ± 0.332
3.612AsnGly: 3.612 ± 0.614
1.227AsnHis: 1.227 ± 0.277
3.68AsnIle: 3.68 ± 0.693
3.544AsnLys: 3.544 ± 0.469
3.544AsnLeu: 3.544 ± 0.479
1.091AsnMet: 1.091 ± 0.342
2.658AsnAsn: 2.658 ± 0.633
1.908AsnPro: 1.908 ± 0.349
1.499AsnGln: 1.499 ± 0.357
1.772AsnArg: 1.772 ± 0.36
3.067AsnSer: 3.067 ± 0.546
3.203AsnThr: 3.203 ± 0.641
3.476AsnVal: 3.476 ± 0.617
0.886AsnTrp: 0.886 ± 0.219
1.499AsnTyr: 1.499 ± 0.32
0.0AsnXaa: 0.0 ± 0.0
Pro
2.794ProAla: 2.794 ± 0.41
0.273ProCys: 0.273 ± 0.131
2.863ProAsp: 2.863 ± 0.488
3.203ProGlu: 3.203 ± 0.473
2.113ProPhe: 2.113 ± 0.341
0.0ProGly: 0.0 ± 0.0
0.613ProHis: 0.613 ± 0.195
2.522ProIle: 2.522 ± 0.396
2.249ProLys: 2.249 ± 0.395
3.544ProLeu: 3.544 ± 0.469
0.75ProMet: 0.75 ± 0.218
1.636ProAsn: 1.636 ± 0.349
1.022ProPro: 1.022 ± 0.248
0.886ProGln: 0.886 ± 0.268
1.363ProArg: 1.363 ± 0.264
2.454ProSer: 2.454 ± 0.489
1.772ProThr: 1.772 ± 0.322
1.977ProVal: 1.977 ± 0.295
0.341ProTrp: 0.341 ± 0.15
0.682ProTyr: 0.682 ± 0.215
0.0ProXaa: 0.0 ± 0.0
Gln
2.385GlnAla: 2.385 ± 0.39
0.477GlnCys: 0.477 ± 0.195
2.317GlnAsp: 2.317 ± 0.391
3.34GlnGlu: 3.34 ± 0.511
1.704GlnPhe: 1.704 ± 0.339
1.977GlnGly: 1.977 ± 0.382
0.75GlnHis: 0.75 ± 0.26
2.249GlnIle: 2.249 ± 0.369
2.522GlnLys: 2.522 ± 0.422
3.885GlnLeu: 3.885 ± 0.528
1.091GlnMet: 1.091 ± 0.255
1.568GlnAsn: 1.568 ± 0.379
1.159GlnPro: 1.159 ± 0.304
1.022GlnGln: 1.022 ± 0.286
2.385GlnArg: 2.385 ± 0.422
1.977GlnSer: 1.977 ± 0.312
1.704GlnThr: 1.704 ± 0.398
2.385GlnVal: 2.385 ± 0.368
0.477GlnTrp: 0.477 ± 0.172
1.568GlnTyr: 1.568 ± 0.429
0.0GlnXaa: 0.0 ± 0.0
Arg
3.476ArgAla: 3.476 ± 0.522
0.273ArgCys: 0.273 ± 0.136
2.726ArgAsp: 2.726 ± 0.402
3.885ArgGlu: 3.885 ± 0.578
2.385ArgPhe: 2.385 ± 0.4
2.522ArgGly: 2.522 ± 0.396
0.818ArgHis: 0.818 ± 0.231
3.135ArgIle: 3.135 ± 0.398
3.476ArgLys: 3.476 ± 0.536
3.272ArgLeu: 3.272 ± 0.562
0.818ArgMet: 0.818 ± 0.218
1.908ArgAsn: 1.908 ± 0.347
1.091ArgPro: 1.091 ± 0.243
2.045ArgGln: 2.045 ± 0.301
3.34ArgArg: 3.34 ± 0.431
2.385ArgSer: 2.385 ± 0.426
2.794ArgThr: 2.794 ± 0.467
2.999ArgVal: 2.999 ± 0.447
0.477ArgTrp: 0.477 ± 0.18
1.84ArgTyr: 1.84 ± 0.415
0.0ArgXaa: 0.0 ± 0.0
Ser
3.408SerAla: 3.408 ± 0.644
0.613SerCys: 0.613 ± 0.253
3.749SerAsp: 3.749 ± 0.593
4.567SerGlu: 4.567 ± 0.538
3.067SerPhe: 3.067 ± 0.369
5.384SerGly: 5.384 ± 0.947
1.636SerHis: 1.636 ± 0.27
4.635SerIle: 4.635 ± 0.518
5.521SerLys: 5.521 ± 0.669
4.975SerLeu: 4.975 ± 0.631
1.363SerMet: 1.363 ± 0.266
3.408SerAsn: 3.408 ± 0.534
2.454SerPro: 2.454 ± 0.441
2.999SerGln: 2.999 ± 0.445
1.977SerArg: 1.977 ± 0.275
4.771SerSer: 4.771 ± 0.68
2.999SerThr: 2.999 ± 0.559
3.408SerVal: 3.408 ± 0.525
1.091SerTrp: 1.091 ± 0.32
2.181SerTyr: 2.181 ± 0.336
0.0SerXaa: 0.0 ± 0.0
Thr
3.953ThrAla: 3.953 ± 0.517
0.409ThrCys: 0.409 ± 0.14
2.863ThrAsp: 2.863 ± 0.484
4.703ThrGlu: 4.703 ± 0.451
2.59ThrPhe: 2.59 ± 0.304
4.907ThrGly: 4.907 ± 0.659
0.613ThrHis: 0.613 ± 0.179
3.749ThrIle: 3.749 ± 0.526
3.476ThrLys: 3.476 ± 0.49
4.43ThrLeu: 4.43 ± 0.594
0.886ThrMet: 0.886 ± 0.26
2.999ThrAsn: 2.999 ± 0.493
3.135ThrPro: 3.135 ± 0.591
1.568ThrGln: 1.568 ± 0.297
2.317ThrArg: 2.317 ± 0.435
3.953ThrSer: 3.953 ± 0.807
3.476ThrThr: 3.476 ± 0.482
4.567ThrVal: 4.567 ± 0.551
0.75ThrTrp: 0.75 ± 0.246
2.931ThrTyr: 2.931 ± 0.53
0.0ThrXaa: 0.0 ± 0.0
Val
4.43ValAla: 4.43 ± 0.647
1.091ValCys: 1.091 ± 0.249
4.43ValAsp: 4.43 ± 0.522
4.158ValGlu: 4.158 ± 0.552
2.726ValPhe: 2.726 ± 0.396
3.272ValGly: 3.272 ± 0.411
1.022ValHis: 1.022 ± 0.249
5.18ValIle: 5.18 ± 0.755
5.657ValLys: 5.657 ± 0.634
4.362ValLeu: 4.362 ± 0.639
1.363ValMet: 1.363 ± 0.242
2.999ValAsn: 2.999 ± 0.534
1.977ValPro: 1.977 ± 0.376
2.999ValGln: 2.999 ± 0.399
2.726ValArg: 2.726 ± 0.36
4.635ValSer: 4.635 ± 0.804
3.953ValThr: 3.953 ± 0.502
3.68ValVal: 3.68 ± 0.615
0.341ValTrp: 0.341 ± 0.175
2.863ValTyr: 2.863 ± 0.381
0.0ValXaa: 0.0 ± 0.0
Trp
0.341TrpAla: 0.341 ± 0.151
0.136TrpCys: 0.136 ± 0.086
0.75TrpAsp: 0.75 ± 0.184
0.682TrpGlu: 0.682 ± 0.245
0.341TrpPhe: 0.341 ± 0.137
0.75TrpGly: 0.75 ± 0.218
0.136TrpHis: 0.136 ± 0.098
0.75TrpIle: 0.75 ± 0.239
0.613TrpLys: 0.613 ± 0.198
0.954TrpLeu: 0.954 ± 0.224
0.545TrpMet: 0.545 ± 0.173
0.682TrpAsn: 0.682 ± 0.209
0.0TrpPro: 0.0 ± 0.0
0.341TrpGln: 0.341 ± 0.15
0.477TrpArg: 0.477 ± 0.202
1.091TrpSer: 1.091 ± 0.302
0.818TrpThr: 0.818 ± 0.242
0.75TrpVal: 0.75 ± 0.296
0.136TrpTrp: 0.136 ± 0.09
0.682TrpTyr: 0.682 ± 0.175
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.908TyrAla: 1.908 ± 0.354
0.341TyrCys: 0.341 ± 0.15
3.067TyrAsp: 3.067 ± 0.448
3.408TyrGlu: 3.408 ± 0.43
1.363TyrPhe: 1.363 ± 0.345
3.953TyrGly: 3.953 ± 0.529
0.682TyrHis: 0.682 ± 0.204
2.726TyrIle: 2.726 ± 0.448
2.658TyrLys: 2.658 ± 0.495
3.067TyrLeu: 3.067 ± 0.43
1.022TyrMet: 1.022 ± 0.284
1.908TyrAsn: 1.908 ± 0.447
0.682TyrPro: 0.682 ± 0.246
1.159TyrGln: 1.159 ± 0.247
1.772TyrArg: 1.772 ± 0.442
2.59TyrSer: 2.59 ± 0.367
3.272TyrThr: 3.272 ± 0.616
2.454TyrVal: 2.454 ± 0.466
0.341TyrTrp: 0.341 ± 0.141
1.227TyrTyr: 1.227 ± 0.287
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 74 proteins (14673 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski