Vagococcus acidifermentans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Vagococcus

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2675 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A430APZ0|A0A430APZ0_9ENTE N-acetyltransferase domain-containing protein OS=Vagococcus acidifermentans OX=564710 GN=CBF27_10760 PE=4 SV=1
MM1 pKa = 7.65PLVKK5 pKa = 9.61IVYY8 pKa = 10.3ASLTGNTEE16 pKa = 3.93EE17 pKa = 4.62IADD20 pKa = 3.64IVAEE24 pKa = 4.0AFEE27 pKa = 5.63DD28 pKa = 3.69NGCEE32 pKa = 4.02VTIDD36 pKa = 3.18EE37 pKa = 4.7CTQVDD42 pKa = 3.3AADD45 pKa = 3.83FLEE48 pKa = 5.43ADD50 pKa = 3.93LCVVAAYY57 pKa = 8.95TYY59 pKa = 11.18DD60 pKa = 4.52DD61 pKa = 3.39GHH63 pKa = 8.53LPDD66 pKa = 5.58EE67 pKa = 4.44IVDD70 pKa = 4.55FYY72 pKa = 11.89EE73 pKa = 4.19EE74 pKa = 4.21LQEE77 pKa = 5.27LDD79 pKa = 3.84LSGKK83 pKa = 9.07IAGVVGSGDD92 pKa = 3.02SFYY95 pKa = 11.0DD96 pKa = 3.67YY97 pKa = 10.83FCKK100 pKa = 10.63SVDD103 pKa = 3.65DD104 pKa = 4.4FEE106 pKa = 4.73QAFAAAGARR115 pKa = 11.84KK116 pKa = 8.87GAASVKK122 pKa = 10.16VEE124 pKa = 4.16LAAEE128 pKa = 4.21EE129 pKa = 4.07EE130 pKa = 4.95DD131 pKa = 3.66IVQLEE136 pKa = 4.44TFASDD141 pKa = 3.26MLRR144 pKa = 11.84TFQAQQ149 pKa = 2.8

Molecular weight: 16.18 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A430AMP7|A0A430AMP7_9ENTE Uncharacterized protein OS=Vagococcus acidifermentans OX=564710 GN=CBF27_13125 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 9.7QPKK8 pKa = 8.83KK9 pKa = 9.05RR10 pKa = 11.84KK11 pKa = 7.43RR12 pKa = 11.84QKK14 pKa = 8.87VHH16 pKa = 5.8GFRR19 pKa = 11.84KK20 pKa = 10.04RR21 pKa = 11.84MSTKK25 pKa = 9.46NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.8GRR39 pKa = 11.84KK40 pKa = 8.76VLAAA44 pKa = 4.31

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2675

0

2675

828011

34

1466

309.5

34.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.702 ± 0.058

0.765 ± 0.015

5.7 ± 0.045

6.832 ± 0.049

4.48 ± 0.039

6.746 ± 0.044

1.971 ± 0.02

7.299 ± 0.04

6.323 ± 0.043

9.999 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.735 ± 0.023

4.174 ± 0.034

3.527 ± 0.021

4.022 ± 0.035

4.165 ± 0.033

5.999 ± 0.044

5.843 ± 0.035

7.218 ± 0.039

0.878 ± 0.017

3.623 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski