Amino acid dipepetide frequency for Streptomyces sp. WM6378

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.537AlaAla: 21.537 ± 0.147	1.105AlaCys: 1.105 ± 0.025	8.166AlaAsp: 8.166 ± 0.074	8.759AlaGlu: 8.759 ± 0.098	3.669AlaPhe: 3.669 ± 0.046	12.793AlaGly: 12.793 ± 0.081	2.954AlaHis: 2.954 ± 0.041	3.764AlaIle: 3.764 ± 0.046	3.291AlaLys: 3.291 ± 0.049	14.787AlaLeu: 14.787 ± 0.115	2.664AlaMet: 2.664 ± 0.039	2.073AlaAsn: 2.073 ± 0.032	7.161AlaPro: 7.161 ± 0.076	4.053AlaGln: 4.053 ± 0.048	9.92AlaArg: 9.92 ± 0.09	6.148AlaSer: 6.148 ± 0.058	7.038AlaThr: 7.038 ± 0.062	12.106AlaVal: 12.106 ± 0.088	1.87AlaTrp: 1.87 ± 0.029	2.843AlaTyr: 2.843 ± 0.037	0.0AlaXaa: 0.0 ± 0.0
Cys	1.121CysAla: 1.121 ± 0.025	0.091CysCys: 0.091 ± 0.006	0.457CysAsp: 0.457 ± 0.016	0.415CysGlu: 0.415 ± 0.015	0.235CysPhe: 0.235 ± 0.01	0.984CysGly: 0.984 ± 0.022	0.197CysHis: 0.197 ± 0.011	0.197CysIle: 0.197 ± 0.01	0.136CysLys: 0.136 ± 0.008	0.789CysLeu: 0.789 ± 0.021	0.141CysMet: 0.141 ± 0.007	0.162CysAsn: 0.162 ± 0.009	0.502CysPro: 0.502 ± 0.015	0.191CysGln: 0.191 ± 0.009	0.619CysArg: 0.619 ± 0.017	0.483CysSer: 0.483 ± 0.016	0.531CysThr: 0.531 ± 0.017	0.674CysVal: 0.674 ± 0.017	0.134CysTrp: 0.134 ± 0.007	0.157CysTyr: 0.157 ± 0.008	0.0CysXaa: 0.0 ± 0.0
Asp	7.607AspAla: 7.607 ± 0.074	0.431AspCys: 0.431 ± 0.014	3.327AspAsp: 3.327 ± 0.045	3.728AspGlu: 3.728 ± 0.046	1.719AspPhe: 1.719 ± 0.027	6.025AspGly: 6.025 ± 0.067	1.408AspHis: 1.408 ± 0.026	1.889AspIle: 1.889 ± 0.028	1.318AspLys: 1.318 ± 0.03	6.04AspLeu: 6.04 ± 0.063	0.79AspMet: 0.79 ± 0.018	1.009AspAsn: 1.009 ± 0.022	4.295AspPro: 4.295 ± 0.046	1.665AspGln: 1.665 ± 0.025	4.565AspArg: 4.565 ± 0.049	2.575AspSer: 2.575 ± 0.039	3.042AspThr: 3.042 ± 0.038	4.481AspVal: 4.481 ± 0.05	1.032AspTrp: 1.032 ± 0.024	1.121AspTyr: 1.121 ± 0.024	0.0AspXaa: 0.0 ± 0.0
Glu	7.28GluAla: 7.28 ± 0.081	0.39GluCys: 0.39 ± 0.013	2.599GluAsp: 2.599 ± 0.04	3.268GluGlu: 3.268 ± 0.046	1.506GluPhe: 1.506 ± 0.027	4.151GluGly: 4.151 ± 0.047	1.481GluHis: 1.481 ± 0.028	2.221GluIle: 2.221 ± 0.035	1.337GluLys: 1.337 ± 0.029	7.082GluLeu: 7.082 ± 0.066	0.878GluMet: 0.878 ± 0.019	0.981GluAsn: 0.981 ± 0.023	3.177GluPro: 3.177 ± 0.044	2.267GluGln: 2.267 ± 0.037	5.239GluArg: 5.239 ± 0.059	2.622GluSer: 2.622 ± 0.032	2.688GluThr: 2.688 ± 0.039	4.286GluVal: 4.286 ± 0.054	0.758GluTrp: 0.758 ± 0.018	1.11GluTyr: 1.11 ± 0.025	0.0GluXaa: 0.0 ± 0.0
Phe	3.88PheAla: 3.88 ± 0.042	0.298PheCys: 0.298 ± 0.012	1.958PheAsp: 1.958 ± 0.032	1.43PheGlu: 1.43 ± 0.028	0.943PhePhe: 0.943 ± 0.023	3.078PheGly: 3.078 ± 0.038	0.648PheHis: 0.648 ± 0.017	0.817PheIle: 0.817 ± 0.025	0.6PheLys: 0.6 ± 0.018	2.637PheLeu: 2.637 ± 0.037	0.429PheMet: 0.429 ± 0.014	0.618PheAsn: 0.618 ± 0.018	1.438PhePro: 1.438 ± 0.024	0.742PheGln: 0.742 ± 0.017	1.776PheArg: 1.776 ± 0.029	1.515PheSer: 1.515 ± 0.029	2.088PheThr: 2.088 ± 0.032	2.245PheVal: 2.245 ± 0.033	0.424PheTrp: 0.424 ± 0.014	0.601PheTyr: 0.601 ± 0.017	0.0PheXaa: 0.0 ± 0.0
Gly	11.001GlyAla: 11.001 ± 0.091	0.895GlyCys: 0.895 ± 0.023	4.929GlyAsp: 4.929 ± 0.046	4.99GlyGlu: 4.99 ± 0.052	2.936GlyPhe: 2.936 ± 0.037	8.604GlyGly: 8.604 ± 0.094	2.411GlyHis: 2.411 ± 0.041	3.704GlyIle: 3.704 ± 0.049	2.605GlyLys: 2.605 ± 0.041	9.484GlyLeu: 9.484 ± 0.076	1.992GlyMet: 1.992 ± 0.029	1.812GlyAsn: 1.812 ± 0.034	5.071GlyPro: 5.071 ± 0.057	2.881GlyGln: 2.881 ± 0.039	7.485GlyArg: 7.485 ± 0.064	5.406GlySer: 5.406 ± 0.053	5.998GlyThr: 5.998 ± 0.059	7.296GlyVal: 7.296 ± 0.058	1.788GlyTrp: 1.788 ± 0.035	2.293GlyTyr: 2.293 ± 0.034	0.0GlyXaa: 0.0 ± 0.0
His	2.671HisAla: 2.671 ± 0.033	0.229HisCys: 0.229 ± 0.01	1.338HisAsp: 1.338 ± 0.027	1.245HisGlu: 1.245 ± 0.026	0.688HisPhe: 0.688 ± 0.021	2.379HisGly: 2.379 ± 0.034	0.698HisHis: 0.698 ± 0.017	0.75HisIle: 0.75 ± 0.016	0.398HisLys: 0.398 ± 0.015	2.475HisLeu: 2.475 ± 0.038	0.371HisMet: 0.371 ± 0.012	0.394HisAsn: 0.394 ± 0.015	1.828HisPro: 1.828 ± 0.03	0.726HisGln: 0.726 ± 0.019	2.04HisArg: 2.04 ± 0.033	1.087HisSer: 1.087 ± 0.025	1.4HisThr: 1.4 ± 0.027	1.686HisVal: 1.686 ± 0.03	0.382HisTrp: 0.382 ± 0.013	0.519HisTyr: 0.519 ± 0.015	0.0HisXaa: 0.0 ± 0.0
Ile	5.127IleAla: 5.127 ± 0.049	0.324IleCys: 0.324 ± 0.012	2.303IleAsp: 2.303 ± 0.038	2.077IleGlu: 2.077 ± 0.038	0.739IlePhe: 0.739 ± 0.02	3.752IleGly: 3.752 ± 0.042	0.673IleHis: 0.673 ± 0.017	0.905IleIle: 0.905 ± 0.023	0.816IleLys: 0.816 ± 0.021	2.579IleLeu: 2.579 ± 0.038	0.468IleMet: 0.468 ± 0.013	0.766IleAsn: 0.766 ± 0.02	1.882IlePro: 1.882 ± 0.029	0.813IleGln: 0.813 ± 0.022	2.258IleArg: 2.258 ± 0.026	1.801IleSer: 1.801 ± 0.029	2.32IleThr: 2.32 ± 0.029	2.766IleVal: 2.766 ± 0.04	0.424IleTrp: 0.424 ± 0.014	0.574IleTyr: 0.574 ± 0.015	0.0IleXaa: 0.0 ± 0.0
Lys	3.208LysAla: 3.208 ± 0.052	0.133LysCys: 0.133 ± 0.008	1.461LysAsp: 1.461 ± 0.032	1.242LysGlu: 1.242 ± 0.03	0.519LysPhe: 0.519 ± 0.016	1.957LysGly: 1.957 ± 0.034	0.49LysHis: 0.49 ± 0.016	0.934LysIle: 0.934 ± 0.022	0.915LysLys: 0.915 ± 0.029	2.229LysLeu: 2.229 ± 0.032	0.446LysMet: 0.446 ± 0.015	0.589LysAsn: 0.589 ± 0.017	1.481LysPro: 1.481 ± 0.03	0.79LysGln: 0.79 ± 0.02	1.485LysArg: 1.485 ± 0.028	1.338LysSer: 1.338 ± 0.026	1.377LysThr: 1.377 ± 0.028	2.029LysVal: 2.029 ± 0.033	0.312LysTrp: 0.312 ± 0.012	0.484LysTyr: 0.484 ± 0.015	0.0LysXaa: 0.0 ± 0.0
Leu	15.67LeuAla: 15.67 ± 0.12	0.883LeuCys: 0.883 ± 0.021	6.755LeuAsp: 6.755 ± 0.057	4.507LeuGlu: 4.507 ± 0.054	2.721LeuPhe: 2.721 ± 0.038	9.501LeuGly: 9.501 ± 0.078	2.355LeuHis: 2.355 ± 0.033	3.509LeuIle: 3.509 ± 0.045	2.227LeuLys: 2.227 ± 0.031	11.241LeuLeu: 11.241 ± 0.095	1.757LeuMet: 1.757 ± 0.03	1.9LeuAsn: 1.9 ± 0.033	6.505LeuPro: 6.505 ± 0.057	2.312LeuGln: 2.312 ± 0.034	8.307LeuArg: 8.307 ± 0.071	5.482LeuSer: 5.482 ± 0.057	6.796LeuThr: 6.796 ± 0.069	8.753LeuVal: 8.753 ± 0.068	1.325LeuTrp: 1.325 ± 0.028	1.915LeuTyr: 1.915 ± 0.029	0.0LeuXaa: 0.0 ± 0.0
Met	2.372MetAla: 2.372 ± 0.034	0.157MetCys: 0.157 ± 0.009	0.925MetAsp: 0.925 ± 0.02	0.788MetGlu: 0.788 ± 0.018	0.464MetPhe: 0.464 ± 0.017	1.397MetGly: 1.397 ± 0.024	0.384MetHis: 0.384 ± 0.012	0.68MetIle: 0.68 ± 0.02	0.446MetLys: 0.446 ± 0.014	1.801MetLeu: 1.801 ± 0.03	0.322MetMet: 0.322 ± 0.013	0.441MetAsn: 0.441 ± 0.014	1.196MetPro: 1.196 ± 0.022	0.463MetGln: 0.463 ± 0.014	1.469MetArg: 1.469 ± 0.023	1.296MetSer: 1.296 ± 0.024	1.452MetThr: 1.452 ± 0.029	1.359MetVal: 1.359 ± 0.026	0.24MetTrp: 0.24 ± 0.009	0.353MetTyr: 0.353 ± 0.012	0.0MetXaa: 0.0 ± 0.0
Asn	2.329AsnAla: 2.329 ± 0.033	0.188AsnCys: 0.188 ± 0.008	0.982AsnAsp: 0.982 ± 0.024	0.819AsnGlu: 0.819 ± 0.018	0.52AsnPhe: 0.52 ± 0.016	2.026AsnGly: 2.026 ± 0.034	0.426AsnHis: 0.426 ± 0.013	0.69AsnIle: 0.69 ± 0.019	0.48AsnLys: 0.48 ± 0.016	1.716AsnLeu: 1.716 ± 0.03	0.344AsnMet: 0.344 ± 0.012	0.467AsnAsn: 0.467 ± 0.015	1.407AsnPro: 1.407 ± 0.025	0.575AsnGln: 0.575 ± 0.016	1.287AsnArg: 1.287 ± 0.025	1.052AsnSer: 1.052 ± 0.024	1.244AsnThr: 1.244 ± 0.029	1.427AsnVal: 1.427 ± 0.029	0.331AsnTrp: 0.331 ± 0.014	0.424AsnTyr: 0.424 ± 0.014	0.0AsnXaa: 0.0 ± 0.0
Pro	8.602ProAla: 8.602 ± 0.081	0.371ProCys: 0.371 ± 0.014	4.279ProAsp: 4.279 ± 0.048	4.087ProGlu: 4.087 ± 0.046	1.531ProPhe: 1.531 ± 0.025	6.451ProGly: 6.451 ± 0.059	1.378ProHis: 1.378 ± 0.025	1.341ProIle: 1.341 ± 0.027	1.342ProLys: 1.342 ± 0.026	5.385ProLeu: 5.385 ± 0.051	1.034ProMet: 1.034 ± 0.022	1.005ProAsn: 1.005 ± 0.022	3.419ProPro: 3.419 ± 0.059	1.948ProGln: 1.948 ± 0.036	3.702ProArg: 3.702 ± 0.048	3.281ProSer: 3.281 ± 0.043	3.108ProThr: 3.108 ± 0.042	5.53ProVal: 5.53 ± 0.058	0.897ProTrp: 0.897 ± 0.019	1.439ProTyr: 1.439 ± 0.026	0.0ProXaa: 0.0 ± 0.0
Gln	3.901GlnAla: 3.901 ± 0.047	0.2GlnCys: 0.2 ± 0.008	1.517GlnAsp: 1.517 ± 0.024	1.459GlnGlu: 1.459 ± 0.027	0.751GlnPhe: 0.751 ± 0.018	2.448GlnGly: 2.448 ± 0.039	0.746GlnHis: 0.746 ± 0.017	1.162GlnIle: 1.162 ± 0.026	0.707GlnLys: 0.707 ± 0.018	3.464GlnLeu: 3.464 ± 0.045	0.526GlnMet: 0.526 ± 0.017	0.561GlnAsn: 0.561 ± 0.019	1.767GlnPro: 1.767 ± 0.037	1.364GlnGln: 1.364 ± 0.032	2.45GlnArg: 2.45 ± 0.032	1.391GlnSer: 1.391 ± 0.024	1.309GlnThr: 1.309 ± 0.023	2.426GlnVal: 2.426 ± 0.038	0.492GlnTrp: 0.492 ± 0.014	0.683GlnTyr: 0.683 ± 0.02	0.0GlnXaa: 0.0 ± 0.0
Arg	9.522ArgAla: 9.522 ± 0.084	0.574ArgCys: 0.574 ± 0.016	4.012ArgAsp: 4.012 ± 0.046	4.52ArgGlu: 4.52 ± 0.05	2.292ArgPhe: 2.292 ± 0.031	5.681ArgGly: 5.681 ± 0.057	2.021ArgHis: 2.021 ± 0.036	3.357ArgIle: 3.357 ± 0.041	1.689ArgLys: 1.689 ± 0.029	8.394ArgLeu: 8.394 ± 0.076	1.705ArgMet: 1.705 ± 0.028	1.348ArgAsn: 1.348 ± 0.023	4.746ArgPro: 4.746 ± 0.053	2.301ArgGln: 2.301 ± 0.033	7.154ArgArg: 7.154 ± 0.066	3.91ArgSer: 3.91 ± 0.043	5.319ArgThr: 5.319 ± 0.042	5.411ArgVal: 5.411 ± 0.049	1.354ArgTrp: 1.354 ± 0.027	1.706ArgTyr: 1.706 ± 0.026	0.0ArgXaa: 0.0 ± 0.0
Ser	7.028SerAla: 7.028 ± 0.073	0.447SerCys: 0.447 ± 0.013	2.683SerAsp: 2.683 ± 0.038	2.336SerGlu: 2.336 ± 0.033	1.594SerPhe: 1.594 ± 0.026	6.0SerGly: 6.0 ± 0.067	1.078SerHis: 1.078 ± 0.025	1.595SerIle: 1.595 ± 0.028	1.12SerLys: 1.12 ± 0.026	5.065SerLeu: 5.065 ± 0.047	1.142SerMet: 1.142 ± 0.02	0.955SerAsn: 0.955 ± 0.02	3.256SerPro: 3.256 ± 0.045	1.341SerGln: 1.341 ± 0.023	3.514SerArg: 3.514 ± 0.041	3.02SerSer: 3.02 ± 0.045	3.143SerThr: 3.143 ± 0.041	4.402SerVal: 4.402 ± 0.045	0.974SerTrp: 0.974 ± 0.023	1.299SerTyr: 1.299 ± 0.025	0.0SerXaa: 0.0 ± 0.0
Thr	8.659ThrAla: 8.659 ± 0.077	0.41ThrCys: 0.41 ± 0.014	3.387ThrAsp: 3.387 ± 0.043	3.1ThrGlu: 3.1 ± 0.038	1.665ThrPhe: 1.665 ± 0.026	6.542ThrGly: 6.542 ± 0.059	1.217ThrHis: 1.217 ± 0.025	1.799ThrIle: 1.799 ± 0.033	1.261ThrLys: 1.261 ± 0.028	5.73ThrLeu: 5.73 ± 0.049	0.984ThrMet: 0.984 ± 0.023	1.061ThrAsn: 1.061 ± 0.023	4.015ThrPro: 4.015 ± 0.049	1.427ThrGln: 1.427 ± 0.025	3.727ThrArg: 3.727 ± 0.042	3.247ThrSer: 3.247 ± 0.04	3.717ThrThr: 3.717 ± 0.054	5.926ThrVal: 5.926 ± 0.056	0.908ThrTrp: 0.908 ± 0.021	1.417ThrTyr: 1.417 ± 0.025	0.0ThrXaa: 0.0 ± 0.0
Val	10.589ValAla: 10.589 ± 0.07	0.741ValCys: 0.741 ± 0.018	4.726ValAsp: 4.726 ± 0.051	4.552ValGlu: 4.552 ± 0.055	2.452ValPhe: 2.452 ± 0.033	6.358ValGly: 6.358 ± 0.062	1.955ValHis: 1.955 ± 0.029	2.954ValIle: 2.954 ± 0.029	1.879ValLys: 1.879 ± 0.037	9.523ValLeu: 9.523 ± 0.077	1.444ValMet: 1.444 ± 0.028	1.731ValAsn: 1.731 ± 0.029	5.09ValPro: 5.09 ± 0.048	2.155ValGln: 2.155 ± 0.032	6.936ValArg: 6.936 ± 0.059	4.373ValSer: 4.373 ± 0.047	5.402ValThr: 5.402 ± 0.046	7.77ValVal: 7.77 ± 0.069	1.061ValTrp: 1.061 ± 0.022	1.567ValTyr: 1.567 ± 0.03	0.0ValXaa: 0.0 ± 0.0
Trp	1.702TrpAla: 1.702 ± 0.03	0.164TrpCys: 0.164 ± 0.009	0.823TrpAsp: 0.823 ± 0.023	0.702TrpGlu: 0.702 ± 0.016	0.521TrpPhe: 0.521 ± 0.016	1.081TrpGly: 1.081 ± 0.022	0.39TrpHis: 0.39 ± 0.015	0.586TrpIle: 0.586 ± 0.016	0.405TrpLys: 0.405 ± 0.014	1.864TrpLeu: 1.864 ± 0.033	0.3TrpMet: 0.3 ± 0.013	0.423TrpAsn: 0.423 ± 0.014	0.807TrpPro: 0.807 ± 0.019	0.661TrpGln: 0.661 ± 0.016	1.296TrpArg: 1.296 ± 0.027	0.96TrpSer: 0.96 ± 0.021	1.04TrpThr: 1.04 ± 0.022	0.982TrpVal: 0.982 ± 0.021	0.357TrpTrp: 0.357 ± 0.014	0.372TrpTyr: 0.372 ± 0.012	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.894TyrAla: 2.894 ± 0.035	0.185TyrCys: 0.185 ± 0.009	1.44TyrAsp: 1.44 ± 0.026	1.296TyrGlu: 1.296 ± 0.023	0.701TyrPhe: 0.701 ± 0.018	2.373TyrGly: 2.373 ± 0.031	0.394TyrHis: 0.394 ± 0.013	0.515TyrIle: 0.515 ± 0.016	0.447TyrLys: 0.447 ± 0.016	2.136TyrLeu: 2.136 ± 0.032	0.265TyrMet: 0.265 ± 0.011	0.437TyrAsn: 0.437 ± 0.016	1.088TyrPro: 1.088 ± 0.021	0.648TyrGln: 0.648 ± 0.016	1.787TyrArg: 1.787 ± 0.03	1.004TyrSer: 1.004 ± 0.022	1.187TyrThr: 1.187 ± 0.025	1.702TyrVal: 1.702 ± 0.024	0.377TyrTrp: 0.377 ± 0.013	0.461TyrTyr: 0.461 ± 0.015	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 7484 proteins (2339543 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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