Eubacteriaceae bacterium

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriaceae; unclassified Eubacteriaceae

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1822 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A425W6V4|A0A425W6V4_9FIRM Rrf2 family transcriptional regulator OS=Eubacteriaceae bacterium OX=2049045 GN=DUD26_03455 PE=4 SV=1
MM1 pKa = 7.67RR2 pKa = 11.84VQAARR7 pKa = 11.84EE8 pKa = 4.0TAPSEE13 pKa = 4.28GGAVTLDD20 pKa = 3.25MTVAEE25 pKa = 5.13IIEE28 pKa = 4.22VHH30 pKa = 6.54PSAADD35 pKa = 3.48YY36 pKa = 11.09LVADD40 pKa = 4.45WNMACISCPASQSEE54 pKa = 4.48TLRR57 pKa = 11.84EE58 pKa = 3.7AAQVHH63 pKa = 5.74EE64 pKa = 5.18LDD66 pKa = 4.86PEE68 pKa = 4.54DD69 pKa = 3.49VCEE72 pKa = 4.23ALNDD76 pKa = 4.03FLEE79 pKa = 4.72DD80 pKa = 2.91TAMIQAEE87 pKa = 4.25EE88 pKa = 4.44LEE90 pKa = 4.33

Molecular weight: 9.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A425W383|A0A425W383_9FIRM Beta sliding clamp OS=Eubacteriaceae bacterium OX=2049045 GN=dnaN PE=3 SV=1
MM1 pKa = 7.31KK2 pKa = 9.53QTFQPKK8 pKa = 8.56NRR10 pKa = 11.84QRR12 pKa = 11.84KK13 pKa = 8.39KK14 pKa = 8.25EE15 pKa = 3.62HH16 pKa = 6.09GFRR19 pKa = 11.84KK20 pKa = 10.0RR21 pKa = 11.84MSTKK25 pKa = 10.25NGRR28 pKa = 11.84LVLKK32 pKa = 10.39RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.8GRR39 pKa = 11.84SKK41 pKa = 11.12LSAA44 pKa = 3.74

Molecular weight: 5.38 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1822

0

1822

595009

31

3444

326.6

36.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.972 ± 0.076

1.342 ± 0.02

6.474 ± 0.049

6.223 ± 0.059

3.867 ± 0.038

7.289 ± 0.053

2.154 ± 0.026

7.066 ± 0.051

6.422 ± 0.056

8.598 ± 0.061

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.918 ± 0.029

3.974 ± 0.039

3.837 ± 0.034

3.576 ± 0.03

4.849 ± 0.052

5.434 ± 0.046

5.536 ± 0.063

7.092 ± 0.046

0.861 ± 0.017

3.515 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski