Candidatus Marinimicrobia bacterium MT.SAG.3

Taxonomy: cellular organisms; Bacteria; FCB group; Candidatus Marinimicrobia

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1570 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y8IED9|A0A4Y8IED9_9BACT Uncharacterized protein OS=Candidatus Marinimicrobia bacterium MT.SAG.3 OX=2552982 GN=E3V55_02365 PE=4 SV=1
MM1 pKa = 7.8LKK3 pKa = 9.07RR4 pKa = 11.84TLLLTLTICAVASLAIPQPAYY25 pKa = 10.58AQISEE30 pKa = 4.32FKK32 pKa = 9.49ITAADD37 pKa = 3.95GAAGDD42 pKa = 4.05LFGNSVSISGDD53 pKa = 3.46YY54 pKa = 10.87AVVGARR60 pKa = 11.84RR61 pKa = 11.84DD62 pKa = 3.64DD63 pKa = 4.25DD64 pKa = 4.33NGISSGSAYY73 pKa = 10.18VYY75 pKa = 10.84KK76 pKa = 10.02RR77 pKa = 11.84TGTSWAQEE85 pKa = 4.02AKK87 pKa = 10.77LLASDD92 pKa = 5.04GAADD96 pKa = 5.72DD97 pKa = 4.19LFGWSVSISGDD108 pKa = 3.57YY109 pKa = 10.87AVVGARR115 pKa = 11.84RR116 pKa = 11.84DD117 pKa = 3.64DD118 pKa = 4.25DD119 pKa = 4.33NGISSGSAYY128 pKa = 10.18VYY130 pKa = 10.84KK131 pKa = 10.02RR132 pKa = 11.84TGTSWAQEE140 pKa = 4.02AKK142 pKa = 10.77LLASDD147 pKa = 5.04GAADD151 pKa = 5.72DD152 pKa = 4.19LFGWSVSISGDD163 pKa = 3.63YY164 pKa = 10.9AVVGAFGDD172 pKa = 4.14DD173 pKa = 4.65DD174 pKa = 4.58NGTDD178 pKa = 3.27AGSAYY183 pKa = 10.15VFKK186 pKa = 10.55RR187 pKa = 11.84TGTSWAQEE195 pKa = 4.02AKK197 pKa = 10.73LLASDD202 pKa = 4.69SGVGDD207 pKa = 3.26QFGNSVSISGDD218 pKa = 3.59YY219 pKa = 10.88AVVGAWLDD227 pKa = 3.86DD228 pKa = 4.11DD229 pKa = 4.95SGSASGSAYY238 pKa = 10.72LFTRR242 pKa = 11.84SGTSWAQKK250 pKa = 10.7AKK252 pKa = 10.79LLASDD257 pKa = 4.58GATEE261 pKa = 4.25GFFGKK266 pKa = 10.02SVSISGDD273 pKa = 3.44YY274 pKa = 10.93AIVGAPAEE282 pKa = 4.5NPGSAYY288 pKa = 10.43LYY290 pKa = 10.59YY291 pKa = 11.03GFTFPQEE298 pKa = 3.76VAMLSFVVEE307 pKa = 3.92PVYY310 pKa = 10.7AVPDD314 pKa = 3.76SDD316 pKa = 4.02SLKK319 pKa = 10.85VNALMASSSGLTLMAAFEE337 pKa = 4.66SVDD340 pKa = 3.58SVLIDD345 pKa = 3.57SVYY348 pKa = 10.99LFDD351 pKa = 6.89DD352 pKa = 4.38DD353 pKa = 4.49AHH355 pKa = 7.01NDD357 pKa = 4.25GIAGDD362 pKa = 4.16SLFGNLWLVPAEE374 pKa = 3.94EE375 pKa = 3.49RR376 pKa = 11.84HH377 pKa = 5.55YY378 pKa = 10.96LVNIHH383 pKa = 5.78VSKK386 pKa = 11.06DD387 pKa = 3.03DD388 pKa = 3.32TLIYY392 pKa = 10.18IRR394 pKa = 11.84EE395 pKa = 3.85NAARR399 pKa = 11.84FTTIGPVVLDD409 pKa = 4.09SYY411 pKa = 10.71TLFDD415 pKa = 4.75TIPNPGEE422 pKa = 3.85RR423 pKa = 11.84LFLEE427 pKa = 3.91LTLRR431 pKa = 11.84NNGSTANATAITADD445 pKa = 3.05ISTSDD450 pKa = 3.38TCVTDD455 pKa = 4.41IGALGQNTYY464 pKa = 11.43GDD466 pKa = 3.92IGPGATATSAGAYY479 pKa = 9.1IVDD482 pKa = 3.93VNEE485 pKa = 4.13NCAGDD490 pKa = 3.82QDD492 pKa = 3.94ILFDD496 pKa = 3.97ISIASEE502 pKa = 4.69GFDD505 pKa = 3.22FWGDD509 pKa = 3.08SFTIHH514 pKa = 6.48VFPTDD519 pKa = 3.11TTIPQTRR526 pKa = 11.84WHH528 pKa = 6.68EE529 pKa = 3.96KK530 pKa = 10.64SIDD533 pKa = 3.82SVDD536 pKa = 3.5GNFSQGVATDD546 pKa = 3.73GVDD549 pKa = 2.88WYY551 pKa = 10.86FSARR555 pKa = 11.84QTLFKK560 pKa = 10.6TNYY563 pKa = 9.85DD564 pKa = 3.29YY565 pKa = 11.88DD566 pKa = 3.91FLVVNEE572 pKa = 4.06NPIPQFLQDD581 pKa = 3.25QSYY584 pKa = 11.34DD585 pKa = 3.71HH586 pKa = 7.23IGDD589 pKa = 3.23IAYY592 pKa = 10.41FEE594 pKa = 4.6GKK596 pKa = 10.02LYY598 pKa = 11.02APIEE602 pKa = 4.38DD603 pKa = 3.51ASYY606 pKa = 10.75VKK608 pKa = 10.25PIICLYY614 pKa = 10.72DD615 pKa = 3.59AVSLDD620 pKa = 3.55FTGEE624 pKa = 4.16FAQVPQSHH632 pKa = 6.52IPWVAVDD639 pKa = 3.44PRR641 pKa = 11.84TGYY644 pKa = 10.36FYY646 pKa = 10.9SSEE649 pKa = 4.07FNGVNNLFVYY659 pKa = 10.44DD660 pKa = 4.17PNQNFALIDD669 pKa = 3.87EE670 pKa = 4.68VQLDD674 pKa = 3.98TTLSNVQGGAFLGDD688 pKa = 3.8FLYY691 pKa = 10.96LAA693 pKa = 5.06

Molecular weight: 74.06 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y8I424|A0A4Y8I424_9BACT Uncharacterized protein OS=Candidatus Marinimicrobia bacterium MT.SAG.3 OX=2552982 GN=E3V55_05775 PE=4 SV=1
MM1 pKa = 7.6AKK3 pKa = 10.02KK4 pKa = 10.25SWIAKK9 pKa = 7.53QKK11 pKa = 8.76KK12 pKa = 4.13TQKK15 pKa = 10.16FAVRR19 pKa = 11.84EE20 pKa = 3.92YY21 pKa = 11.02NRR23 pKa = 11.84CQRR26 pKa = 11.84CGRR29 pKa = 11.84ARR31 pKa = 11.84SYY33 pKa = 10.81YY34 pKa = 10.18RR35 pKa = 11.84RR36 pKa = 11.84FGLCRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.87LALKK50 pKa = 10.94GEE52 pKa = 4.37IPGITKK58 pKa = 10.6ASWW61 pKa = 2.73

Molecular weight: 7.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1570

0

1570

510553

25

2012

325.2

36.33

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.711 ± 0.056

0.61 ± 0.017

5.897 ± 0.052

7.316 ± 0.074

4.574 ± 0.05

7.368 ± 0.061

1.669 ± 0.022

8.134 ± 0.057

6.605 ± 0.072

9.759 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.497 ± 0.035

4.598 ± 0.044

3.639 ± 0.034

2.48 ± 0.026

4.587 ± 0.046

7.418 ± 0.054

5.173 ± 0.047

6.434 ± 0.045

1.065 ± 0.023

3.467 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski