Amino acid dipepetide frequency for Vibrio phage VP2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.666AlaAla: 8.666 ± 1.523
0.774AlaCys: 0.774 ± 0.446
5.571AlaAsp: 5.571 ± 0.974
4.797AlaGlu: 4.797 ± 1.078
2.012AlaPhe: 2.012 ± 0.189
8.202AlaGly: 8.202 ± 1.27
0.929AlaHis: 0.929 ± 0.449
5.881AlaIle: 5.881 ± 1.054
4.333AlaLys: 4.333 ± 1.0
7.892AlaLeu: 7.892 ± 1.017
3.095AlaMet: 3.095 ± 0.516
3.095AlaAsn: 3.095 ± 0.455
3.25AlaPro: 3.25 ± 0.552
2.94AlaGln: 2.94 ± 0.607
3.405AlaArg: 3.405 ± 0.685
6.809AlaSer: 6.809 ± 0.99
5.726AlaThr: 5.726 ± 0.871
5.881AlaVal: 5.881 ± 0.924
1.238AlaTrp: 1.238 ± 0.404
2.94AlaTyr: 2.94 ± 0.83
0.0AlaXaa: 0.0 ± 0.0
Cys
0.619CysAla: 0.619 ± 0.276
0.464CysCys: 0.464 ± 0.228
0.31CysAsp: 0.31 ± 0.206
0.774CysGlu: 0.774 ± 0.253
0.619CysPhe: 0.619 ± 0.188
1.083CysGly: 1.083 ± 0.336
0.155CysHis: 0.155 ± 0.161
1.238CysIle: 1.238 ± 0.372
0.464CysLys: 0.464 ± 0.27
0.929CysLeu: 0.929 ± 0.308
0.0CysMet: 0.0 ± 0.0
0.155CysAsn: 0.155 ± 0.129
0.31CysPro: 0.31 ± 0.248
0.31CysGln: 0.31 ± 0.174
0.464CysArg: 0.464 ± 0.295
0.155CysSer: 0.155 ± 0.15
0.929CysThr: 0.929 ± 0.376
0.31CysVal: 0.31 ± 0.195
0.155CysTrp: 0.155 ± 0.15
0.0CysTyr: 0.0 ± 0.0
0.0CysXaa: 0.0 ± 0.0
Asp
4.797AspAla: 4.797 ± 0.835
1.083AspCys: 1.083 ± 0.436
3.559AspAsp: 3.559 ± 0.925
4.643AspGlu: 4.643 ± 1.222
3.095AspPhe: 3.095 ± 0.632
6.345AspGly: 6.345 ± 1.54
0.464AspHis: 0.464 ± 0.306
4.488AspIle: 4.488 ± 0.674
4.333AspLys: 4.333 ± 1.072
4.643AspLeu: 4.643 ± 0.84
1.857AspMet: 1.857 ± 0.686
2.476AspAsn: 2.476 ± 0.606
2.94AspPro: 2.94 ± 0.707
0.929AspGln: 0.929 ± 0.431
2.94AspArg: 2.94 ± 0.881
5.726AspSer: 5.726 ± 0.691
3.559AspThr: 3.559 ± 0.952
4.024AspVal: 4.024 ± 0.663
2.012AspTrp: 2.012 ± 0.469
1.548AspTyr: 1.548 ± 0.57
0.0AspXaa: 0.0 ± 0.0
Glu
4.024GluAla: 4.024 ± 0.759
0.774GluCys: 0.774 ± 0.322
3.869GluAsp: 3.869 ± 0.759
4.643GluGlu: 4.643 ± 0.962
2.012GluPhe: 2.012 ± 0.495
3.405GluGly: 3.405 ± 0.831
1.548GluHis: 1.548 ± 0.629
2.476GluIle: 2.476 ± 0.821
2.321GluLys: 2.321 ± 0.456
4.178GluLeu: 4.178 ± 0.95
1.548GluMet: 1.548 ± 0.486
2.476GluAsn: 2.476 ± 0.959
1.702GluPro: 1.702 ± 0.452
3.559GluGln: 3.559 ± 0.829
3.405GluArg: 3.405 ± 1.066
2.786GluSer: 2.786 ± 0.707
2.631GluThr: 2.631 ± 0.529
4.333GluVal: 4.333 ± 0.79
1.702GluTrp: 1.702 ± 0.529
2.167GluTyr: 2.167 ± 0.551
0.0GluXaa: 0.0 ± 0.0
Phe
2.786PheAla: 2.786 ± 0.841
0.155PheCys: 0.155 ± 0.15
2.94PheAsp: 2.94 ± 0.633
1.702PheGlu: 1.702 ± 0.542
0.774PhePhe: 0.774 ± 0.449
4.178PheGly: 4.178 ± 1.078
0.155PheHis: 0.155 ± 0.155
1.702PheIle: 1.702 ± 0.43
1.548PheLys: 1.548 ± 0.813
2.631PheLeu: 2.631 ± 0.486
1.083PheMet: 1.083 ± 0.567
2.94PheAsn: 2.94 ± 0.484
1.393PhePro: 1.393 ± 0.39
1.548PheGln: 1.548 ± 0.509
1.857PheArg: 1.857 ± 0.566
1.702PheSer: 1.702 ± 0.657
2.94PheThr: 2.94 ± 0.645
2.94PheVal: 2.94 ± 0.846
0.31PheTrp: 0.31 ± 0.243
0.774PheTyr: 0.774 ± 0.288
0.0PheXaa: 0.0 ± 0.0
Gly
7.273GlyAla: 7.273 ± 1.394
0.774GlyCys: 0.774 ± 0.416
5.881GlyAsp: 5.881 ± 1.052
2.94GlyGlu: 2.94 ± 0.348
2.94GlyPhe: 2.94 ± 0.764
13.154GlyGly: 13.154 ± 5.429
1.393GlyHis: 1.393 ± 0.548
4.643GlyIle: 4.643 ± 0.62
5.881GlyLys: 5.881 ± 0.805
5.262GlyLeu: 5.262 ± 1.011
2.631GlyMet: 2.631 ± 0.922
7.738GlyAsn: 7.738 ± 2.191
2.631GlyPro: 2.631 ± 0.514
3.559GlyGln: 3.559 ± 0.457
3.559GlyArg: 3.559 ± 0.686
6.654GlySer: 6.654 ± 1.545
6.5GlyThr: 6.5 ± 1.215
6.19GlyVal: 6.19 ± 1.408
1.393GlyTrp: 1.393 ± 0.301
3.559GlyTyr: 3.559 ± 0.514
0.0GlyXaa: 0.0 ± 0.0
His
0.619HisAla: 0.619 ± 0.347
0.31HisCys: 0.31 ± 0.189
1.393HisAsp: 1.393 ± 0.393
1.083HisGlu: 1.083 ± 0.416
0.31HisPhe: 0.31 ± 0.249
1.083HisGly: 1.083 ± 0.324
0.155HisHis: 0.155 ± 0.15
1.548HisIle: 1.548 ± 0.533
0.155HisLys: 0.155 ± 0.132
1.083HisLeu: 1.083 ± 0.41
0.929HisMet: 0.929 ± 0.523
0.774HisAsn: 0.774 ± 0.24
0.464HisPro: 0.464 ± 0.223
0.31HisGln: 0.31 ± 0.206
1.083HisArg: 1.083 ± 0.491
1.238HisSer: 1.238 ± 0.471
0.464HisThr: 0.464 ± 0.285
1.393HisVal: 1.393 ± 0.426
0.0HisTrp: 0.0 ± 0.0
0.619HisTyr: 0.619 ± 0.449
0.0HisXaa: 0.0 ± 0.0
Ile
7.119IleAla: 7.119 ± 1.015
0.31IleCys: 0.31 ± 0.171
5.262IleAsp: 5.262 ± 0.767
2.786IleGlu: 2.786 ± 0.619
1.083IlePhe: 1.083 ± 0.573
4.488IleGly: 4.488 ± 0.844
0.155IleHis: 0.155 ± 0.143
2.167IleIle: 2.167 ± 0.451
2.476IleLys: 2.476 ± 0.507
2.94IleLeu: 2.94 ± 0.646
1.857IleMet: 1.857 ± 0.569
2.476IleAsn: 2.476 ± 0.667
3.25IlePro: 3.25 ± 0.425
2.94IleGln: 2.94 ± 0.717
3.869IleArg: 3.869 ± 0.634
4.643IleSer: 4.643 ± 0.958
2.786IleThr: 2.786 ± 0.887
3.405IleVal: 3.405 ± 0.482
1.238IleTrp: 1.238 ± 0.482
0.929IleTyr: 0.929 ± 0.352
0.0IleXaa: 0.0 ± 0.0
Lys
4.952LysAla: 4.952 ± 0.831
0.155LysCys: 0.155 ± 0.155
2.167LysAsp: 2.167 ± 0.637
2.631LysGlu: 2.631 ± 0.658
1.548LysPhe: 1.548 ± 0.542
5.416LysGly: 5.416 ± 1.368
1.238LysHis: 1.238 ± 0.371
2.167LysIle: 2.167 ± 0.416
4.178LysLys: 4.178 ± 1.021
4.024LysLeu: 4.024 ± 0.742
2.476LysMet: 2.476 ± 0.752
2.476LysAsn: 2.476 ± 0.607
2.012LysPro: 2.012 ± 0.661
1.857LysGln: 1.857 ± 0.494
2.631LysArg: 2.631 ± 0.675
3.714LysSer: 3.714 ± 0.752
2.012LysThr: 2.012 ± 0.665
3.095LysVal: 3.095 ± 0.657
0.929LysTrp: 0.929 ± 0.461
2.786LysTyr: 2.786 ± 0.874
0.0LysXaa: 0.0 ± 0.0
Leu
5.107LeuAla: 5.107 ± 0.721
0.619LeuCys: 0.619 ± 0.391
3.714LeuAsp: 3.714 ± 0.785
4.797LeuGlu: 4.797 ± 1.051
1.702LeuPhe: 1.702 ± 0.627
6.345LeuGly: 6.345 ± 0.941
1.702LeuHis: 1.702 ± 0.594
3.405LeuIle: 3.405 ± 0.703
3.25LeuLys: 3.25 ± 0.743
2.631LeuLeu: 2.631 ± 0.491
2.786LeuMet: 2.786 ± 0.846
3.095LeuAsn: 3.095 ± 0.79
4.024LeuPro: 4.024 ± 0.877
2.476LeuGln: 2.476 ± 0.496
4.178LeuArg: 4.178 ± 0.615
4.488LeuSer: 4.488 ± 0.756
4.488LeuThr: 4.488 ± 0.678
4.797LeuVal: 4.797 ± 0.937
1.393LeuTrp: 1.393 ± 0.608
1.238LeuTyr: 1.238 ± 0.304
0.0LeuXaa: 0.0 ± 0.0
Met
2.631MetAla: 2.631 ± 1.414
0.464MetCys: 0.464 ± 0.245
2.321MetAsp: 2.321 ± 0.416
1.857MetGlu: 1.857 ± 0.52
1.083MetPhe: 1.083 ± 0.346
1.393MetGly: 1.393 ± 0.508
0.464MetHis: 0.464 ± 0.27
1.548MetIle: 1.548 ± 0.347
1.393MetLys: 1.393 ± 0.351
1.393MetLeu: 1.393 ± 0.6
0.774MetMet: 0.774 ± 0.47
1.393MetAsn: 1.393 ± 0.453
2.012MetPro: 2.012 ± 0.585
0.929MetGln: 0.929 ± 0.382
1.857MetArg: 1.857 ± 0.572
2.167MetSer: 2.167 ± 0.693
1.393MetThr: 1.393 ± 0.444
2.476MetVal: 2.476 ± 0.723
0.0MetTrp: 0.0 ± 0.0
1.238MetTyr: 1.238 ± 0.469
0.0MetXaa: 0.0 ± 0.0
Asn
4.952AsnAla: 4.952 ± 1.263
0.464AsnCys: 0.464 ± 0.234
2.631AsnAsp: 2.631 ± 0.837
2.167AsnGlu: 2.167 ± 0.475
1.857AsnPhe: 1.857 ± 0.3
7.428AsnGly: 7.428 ± 1.97
0.31AsnHis: 0.31 ± 0.215
2.786AsnIle: 2.786 ± 0.579
3.25AsnLys: 3.25 ± 0.512
2.012AsnLeu: 2.012 ± 0.545
0.929AsnMet: 0.929 ± 0.445
2.476AsnAsn: 2.476 ± 0.726
2.321AsnPro: 2.321 ± 0.726
2.012AsnGln: 2.012 ± 0.533
3.559AsnArg: 3.559 ± 0.791
2.94AsnSer: 2.94 ± 0.814
2.94AsnThr: 2.94 ± 0.759
2.012AsnVal: 2.012 ± 0.557
2.012AsnTrp: 2.012 ± 0.835
1.548AsnTyr: 1.548 ± 0.556
0.0AsnXaa: 0.0 ± 0.0
Pro
4.333ProAla: 4.333 ± 0.907
0.31ProCys: 0.31 ± 0.204
3.869ProAsp: 3.869 ± 0.639
1.857ProGlu: 1.857 ± 0.487
1.857ProPhe: 1.857 ± 0.409
0.31ProGly: 0.31 ± 0.19
0.774ProHis: 0.774 ± 0.372
2.631ProIle: 2.631 ± 0.47
2.012ProLys: 2.012 ± 0.494
2.167ProLeu: 2.167 ± 0.699
1.083ProMet: 1.083 ± 0.403
3.25ProAsn: 3.25 ± 0.605
1.857ProPro: 1.857 ± 0.818
1.083ProGln: 1.083 ± 0.221
2.631ProArg: 2.631 ± 0.631
3.405ProSer: 3.405 ± 0.582
3.714ProThr: 3.714 ± 0.784
2.786ProVal: 2.786 ± 0.841
0.619ProTrp: 0.619 ± 0.342
1.393ProTyr: 1.393 ± 0.805
0.0ProXaa: 0.0 ± 0.0
Gln
3.405GlnAla: 3.405 ± 0.745
0.155GlnCys: 0.155 ± 0.129
2.167GlnAsp: 2.167 ± 0.555
2.476GlnGlu: 2.476 ± 0.698
1.238GlnPhe: 1.238 ± 0.379
2.786GlnGly: 2.786 ± 0.571
0.464GlnHis: 0.464 ± 0.281
2.631GlnIle: 2.631 ± 0.549
1.393GlnLys: 1.393 ± 0.438
2.94GlnLeu: 2.94 ± 0.672
0.929GlnMet: 0.929 ± 0.558
1.548GlnAsn: 1.548 ± 0.541
1.548GlnPro: 1.548 ± 0.454
1.548GlnGln: 1.548 ± 0.463
2.631GlnArg: 2.631 ± 0.915
2.012GlnSer: 2.012 ± 0.472
3.095GlnThr: 3.095 ± 0.485
1.857GlnVal: 1.857 ± 0.545
1.548GlnTrp: 1.548 ± 0.356
0.929GlnTyr: 0.929 ± 0.343
0.0GlnXaa: 0.0 ± 0.0
Arg
5.881ArgAla: 5.881 ± 0.676
0.464ArgCys: 0.464 ± 0.255
2.631ArgAsp: 2.631 ± 0.365
3.25ArgGlu: 3.25 ± 0.631
2.94ArgPhe: 2.94 ± 1.013
4.643ArgGly: 4.643 ± 1.297
0.31ArgHis: 0.31 ± 0.222
2.321ArgIle: 2.321 ± 0.544
3.559ArgLys: 3.559 ± 0.797
4.643ArgLeu: 4.643 ± 1.062
2.476ArgMet: 2.476 ± 0.498
3.714ArgAsn: 3.714 ± 0.725
2.167ArgPro: 2.167 ± 0.648
1.857ArgGln: 1.857 ± 0.496
2.94ArgArg: 2.94 ± 0.494
3.25ArgSer: 3.25 ± 0.852
2.631ArgThr: 2.631 ± 0.805
2.476ArgVal: 2.476 ± 0.525
1.857ArgTrp: 1.857 ± 0.532
2.321ArgTyr: 2.321 ± 0.574
0.0ArgXaa: 0.0 ± 0.0
Ser
5.262SerAla: 5.262 ± 1.075
0.155SerCys: 0.155 ± 0.143
5.416SerAsp: 5.416 ± 0.988
3.559SerGlu: 3.559 ± 0.659
3.25SerPhe: 3.25 ± 0.496
7.119SerGly: 7.119 ± 1.799
1.083SerHis: 1.083 ± 0.335
4.178SerIle: 4.178 ± 0.934
3.559SerLys: 3.559 ± 1.059
3.869SerLeu: 3.869 ± 0.597
1.702SerMet: 1.702 ± 0.558
4.024SerAsn: 4.024 ± 1.096
3.559SerPro: 3.559 ± 0.695
2.167SerGln: 2.167 ± 0.3
3.559SerArg: 3.559 ± 0.694
4.643SerSer: 4.643 ± 1.28
2.631SerThr: 2.631 ± 0.66
4.333SerVal: 4.333 ± 0.741
1.083SerTrp: 1.083 ± 0.342
2.786SerTyr: 2.786 ± 0.681
0.0SerXaa: 0.0 ± 0.0
Thr
5.571ThrAla: 5.571 ± 1.062
0.31ThrCys: 0.31 ± 0.194
3.714ThrAsp: 3.714 ± 0.875
3.095ThrGlu: 3.095 ± 0.526
3.405ThrPhe: 3.405 ± 0.678
6.5ThrGly: 6.5 ± 0.997
0.774ThrHis: 0.774 ± 0.346
3.559ThrIle: 3.559 ± 0.682
2.94ThrLys: 2.94 ± 0.689
4.797ThrLeu: 4.797 ± 0.768
0.619ThrMet: 0.619 ± 0.357
2.167ThrAsn: 2.167 ± 0.592
2.94ThrPro: 2.94 ± 0.576
2.167ThrGln: 2.167 ± 0.587
3.095ThrArg: 3.095 ± 0.471
3.559ThrSer: 3.559 ± 0.844
2.631ThrThr: 2.631 ± 0.706
3.405ThrVal: 3.405 ± 0.972
1.238ThrTrp: 1.238 ± 0.886
1.393ThrTyr: 1.393 ± 0.386
0.0ThrXaa: 0.0 ± 0.0
Val
4.488ValAla: 4.488 ± 0.726
0.774ValCys: 0.774 ± 0.344
4.024ValAsp: 4.024 ± 0.916
3.405ValGlu: 3.405 ± 0.463
2.167ValPhe: 2.167 ± 0.632
6.964ValGly: 6.964 ± 1.328
2.321ValHis: 2.321 ± 0.58
3.25ValIle: 3.25 ± 1.073
2.012ValLys: 2.012 ± 0.446
4.643ValLeu: 4.643 ± 0.882
1.702ValMet: 1.702 ± 0.476
1.857ValAsn: 1.857 ± 0.863
2.167ValPro: 2.167 ± 0.571
2.476ValGln: 2.476 ± 0.846
4.797ValArg: 4.797 ± 1.101
4.178ValSer: 4.178 ± 1.436
3.869ValThr: 3.869 ± 1.146
5.881ValVal: 5.881 ± 1.059
1.548ValTrp: 1.548 ± 0.397
3.405ValTyr: 3.405 ± 0.811
0.0ValXaa: 0.0 ± 0.0
Trp
1.702TrpAla: 1.702 ± 0.295
0.464TrpCys: 0.464 ± 0.242
1.393TrpAsp: 1.393 ± 0.559
1.238TrpGlu: 1.238 ± 0.521
1.083TrpPhe: 1.083 ± 0.681
0.774TrpGly: 0.774 ± 0.301
0.155TrpHis: 0.155 ± 0.145
1.238TrpIle: 1.238 ± 0.385
0.774TrpLys: 0.774 ± 0.272
1.702TrpLeu: 1.702 ± 0.728
0.155TrpMet: 0.155 ± 0.177
0.31TrpAsn: 0.31 ± 0.19
0.31TrpPro: 0.31 ± 0.285
0.929TrpGln: 0.929 ± 0.375
2.012TrpArg: 2.012 ± 0.456
2.167TrpSer: 2.167 ± 0.675
1.548TrpThr: 1.548 ± 0.33
1.702TrpVal: 1.702 ± 0.559
0.464TrpTrp: 0.464 ± 0.198
0.929TrpTyr: 0.929 ± 0.269
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.25TyrAla: 3.25 ± 0.663
0.464TyrCys: 0.464 ± 0.254
2.631TyrAsp: 2.631 ± 0.443
1.857TyrGlu: 1.857 ± 0.632
1.083TyrPhe: 1.083 ± 0.392
2.94TyrGly: 2.94 ± 0.566
0.619TyrHis: 0.619 ± 0.251
2.321TyrIle: 2.321 ± 0.667
2.631TyrLys: 2.631 ± 0.744
1.857TyrLeu: 1.857 ± 0.574
0.0TyrMet: 0.0 ± 0.0
2.167TyrAsn: 2.167 ± 0.535
1.083TyrPro: 1.083 ± 0.346
1.702TyrGln: 1.702 ± 0.421
1.857TyrArg: 1.857 ± 0.399
1.702TyrSer: 1.702 ± 0.566
1.393TyrThr: 1.393 ± 0.353
2.631TyrVal: 2.631 ± 0.657
0.31TyrTrp: 0.31 ± 0.223
1.393TyrTyr: 1.393 ± 0.552
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 14 proteins (6463 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski