Amino acid dipepetide frequency for Pseudoalteromonas phage PHS3

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
9.658AlaAla: 9.658 ± 2.031
0.508AlaCys: 0.508 ± 0.227
4.575AlaAsp: 4.575 ± 0.862
6.202AlaGlu: 6.202 ± 1.265
2.237AlaPhe: 2.237 ± 0.478
5.083AlaGly: 5.083 ± 0.993
0.915AlaHis: 0.915 ± 0.318
6.913AlaIle: 6.913 ± 1.05
6.405AlaLys: 6.405 ± 0.965
7.93AlaLeu: 7.93 ± 0.893
4.168AlaMet: 4.168 ± 0.835
4.27AlaAsn: 4.27 ± 0.947
2.847AlaPro: 2.847 ± 0.607
2.338AlaGln: 2.338 ± 0.69
3.863AlaArg: 3.863 ± 0.698
5.388AlaSer: 5.388 ± 0.613
4.677AlaThr: 4.677 ± 0.848
5.795AlaVal: 5.795 ± 0.59
0.305AlaTrp: 0.305 ± 0.175
2.135AlaTyr: 2.135 ± 0.637
0.0AlaXaa: 0.0 ± 0.0
Cys
0.712CysAla: 0.712 ± 0.278
0.203CysCys: 0.203 ± 0.145
0.61CysAsp: 0.61 ± 0.253
1.017CysGlu: 1.017 ± 0.328
0.407CysPhe: 0.407 ± 0.206
1.017CysGly: 1.017 ± 0.354
0.305CysHis: 0.305 ± 0.227
0.61CysIle: 0.61 ± 0.247
0.305CysLys: 0.305 ± 0.145
1.118CysLeu: 1.118 ± 0.329
0.407CysMet: 0.407 ± 0.189
0.407CysAsn: 0.407 ± 0.2
0.407CysPro: 0.407 ± 0.205
0.407CysGln: 0.407 ± 0.226
0.712CysArg: 0.712 ± 0.312
0.203CysSer: 0.203 ± 0.14
0.61CysThr: 0.61 ± 0.243
0.407CysVal: 0.407 ± 0.213
0.508CysTrp: 0.508 ± 0.235
0.407CysTyr: 0.407 ± 0.192
0.0CysXaa: 0.0 ± 0.0
Asp
5.083AspAla: 5.083 ± 0.697
0.915AspCys: 0.915 ± 0.302
4.372AspAsp: 4.372 ± 0.741
4.575AspGlu: 4.575 ± 0.738
3.152AspPhe: 3.152 ± 0.578
5.287AspGly: 5.287 ± 0.756
0.915AspHis: 0.915 ± 0.309
3.863AspIle: 3.863 ± 0.78
3.762AspLys: 3.762 ± 0.683
5.897AspLeu: 5.897 ± 0.873
1.423AspMet: 1.423 ± 0.317
3.152AspAsn: 3.152 ± 0.454
2.033AspPro: 2.033 ± 0.523
1.932AspGln: 1.932 ± 0.372
2.033AspArg: 2.033 ± 0.369
4.168AspSer: 4.168 ± 0.632
2.745AspThr: 2.745 ± 0.419
2.542AspVal: 2.542 ± 0.547
1.525AspTrp: 1.525 ± 0.423
3.05AspTyr: 3.05 ± 0.587
0.0AspXaa: 0.0 ± 0.0
Glu
5.795GluAla: 5.795 ± 1.101
0.712GluCys: 0.712 ± 0.269
3.355GluAsp: 3.355 ± 0.599
3.863GluGlu: 3.863 ± 0.92
2.44GluPhe: 2.44 ± 0.454
3.355GluGly: 3.355 ± 0.64
1.83GluHis: 1.83 ± 0.471
5.49GluIle: 5.49 ± 0.703
5.388GluLys: 5.388 ± 1.016
10.675GluLeu: 10.675 ± 1.171
1.932GluMet: 1.932 ± 0.421
3.253GluAsn: 3.253 ± 0.553
1.017GluPro: 1.017 ± 0.31
3.355GluGln: 3.355 ± 0.567
3.863GluArg: 3.863 ± 1.213
4.372GluSer: 4.372 ± 0.665
2.948GluThr: 2.948 ± 0.515
4.168GluVal: 4.168 ± 0.663
1.22GluTrp: 1.22 ± 0.277
2.847GluTyr: 2.847 ± 0.642
0.0GluXaa: 0.0 ± 0.0
Phe
2.948PheAla: 2.948 ± 0.571
0.813PheCys: 0.813 ± 0.259
2.948PheAsp: 2.948 ± 0.599
2.338PheGlu: 2.338 ± 0.482
1.322PhePhe: 1.322 ± 0.38
2.948PheGly: 2.948 ± 0.517
0.305PheHis: 0.305 ± 0.184
2.44PheIle: 2.44 ± 0.365
2.745PheLys: 2.745 ± 0.555
2.135PheLeu: 2.135 ± 0.356
1.118PheMet: 1.118 ± 0.348
2.237PheAsn: 2.237 ± 0.618
0.61PhePro: 0.61 ± 0.196
0.915PheGln: 0.915 ± 0.253
1.017PheArg: 1.017 ± 0.316
3.05PheSer: 3.05 ± 0.514
3.05PheThr: 3.05 ± 0.594
1.728PheVal: 1.728 ± 0.377
0.305PheTrp: 0.305 ± 0.185
1.322PheTyr: 1.322 ± 0.296
0.0PheXaa: 0.0 ± 0.0
Gly
6.507GlyAla: 6.507 ± 1.224
0.305GlyCys: 0.305 ± 0.157
4.677GlyAsp: 4.677 ± 0.81
5.083GlyGlu: 5.083 ± 0.678
2.847GlyPhe: 2.847 ± 0.446
5.897GlyGly: 5.897 ± 2.127
0.305GlyHis: 0.305 ± 0.18
3.457GlyIle: 3.457 ± 0.704
4.982GlyLys: 4.982 ± 0.803
5.49GlyLeu: 5.49 ± 0.777
1.423GlyMet: 1.423 ± 0.313
2.542GlyAsn: 2.542 ± 0.463
0.305GlyPro: 0.305 ± 0.156
2.745GlyGln: 2.745 ± 0.587
1.627GlyArg: 1.627 ± 0.458
4.27GlySer: 4.27 ± 0.898
3.457GlyThr: 3.457 ± 0.779
5.693GlyVal: 5.693 ± 0.69
0.61GlyTrp: 0.61 ± 0.248
2.44GlyTyr: 2.44 ± 0.686
0.0GlyXaa: 0.0 ± 0.0
His
1.017HisAla: 1.017 ± 0.323
0.0HisCys: 0.0 ± 0.0
0.407HisAsp: 0.407 ± 0.259
0.915HisGlu: 0.915 ± 0.331
0.407HisPhe: 0.407 ± 0.201
0.813HisGly: 0.813 ± 0.294
0.407HisHis: 0.407 ± 0.266
1.22HisIle: 1.22 ± 0.376
1.525HisLys: 1.525 ± 0.473
1.525HisLeu: 1.525 ± 0.425
0.203HisMet: 0.203 ± 0.139
0.813HisAsn: 0.813 ± 0.256
0.508HisPro: 0.508 ± 0.2
0.305HisGln: 0.305 ± 0.168
1.322HisArg: 1.322 ± 0.345
0.508HisSer: 0.508 ± 0.214
0.61HisThr: 0.61 ± 0.214
0.508HisVal: 0.508 ± 0.216
0.203HisTrp: 0.203 ± 0.122
0.712HisTyr: 0.712 ± 0.252
0.0HisXaa: 0.0 ± 0.0
Ile
4.677IleAla: 4.677 ± 0.581
0.61IleCys: 0.61 ± 0.322
5.388IleAsp: 5.388 ± 0.765
6.1IleGlu: 6.1 ± 0.769
1.017IlePhe: 1.017 ± 0.355
3.152IleGly: 3.152 ± 0.534
0.61IleHis: 0.61 ± 0.247
3.05IleIle: 3.05 ± 0.591
4.778IleLys: 4.778 ± 0.834
3.965IleLeu: 3.965 ± 0.559
1.118IleMet: 1.118 ± 0.287
4.372IleAsn: 4.372 ± 0.789
2.135IlePro: 2.135 ± 0.391
1.932IleGln: 1.932 ± 0.408
4.168IleArg: 4.168 ± 0.64
4.372IleSer: 4.372 ± 0.59
5.795IleThr: 5.795 ± 0.788
2.847IleVal: 2.847 ± 0.678
0.61IleTrp: 0.61 ± 0.225
2.643IleTyr: 2.643 ± 0.516
0.0IleXaa: 0.0 ± 0.0
Lys
6.913LysAla: 6.913 ± 1.05
0.915LysCys: 0.915 ± 0.276
3.05LysAsp: 3.05 ± 0.446
5.185LysGlu: 5.185 ± 0.715
1.932LysPhe: 1.932 ± 0.495
4.067LysGly: 4.067 ± 0.735
1.22LysHis: 1.22 ± 0.384
3.558LysIle: 3.558 ± 0.755
6.202LysLys: 6.202 ± 0.811
6.71LysLeu: 6.71 ± 0.864
3.253LysMet: 3.253 ± 0.634
3.66LysAsn: 3.66 ± 0.59
3.05LysPro: 3.05 ± 0.663
3.863LysGln: 3.863 ± 0.752
3.355LysArg: 3.355 ± 0.757
5.287LysSer: 5.287 ± 0.791
4.575LysThr: 4.575 ± 0.652
3.965LysVal: 3.965 ± 0.547
1.22LysTrp: 1.22 ± 0.329
2.948LysTyr: 2.948 ± 0.629
0.0LysXaa: 0.0 ± 0.0
Leu
5.693LeuAla: 5.693 ± 0.846
0.915LeuCys: 0.915 ± 0.299
6.1LeuAsp: 6.1 ± 0.68
6.1LeuGlu: 6.1 ± 0.63
2.338LeuPhe: 2.338 ± 0.356
4.473LeuGly: 4.473 ± 0.76
0.712LeuHis: 0.712 ± 0.283
6.608LeuIle: 6.608 ± 0.715
7.523LeuLys: 7.523 ± 0.803
5.49LeuLeu: 5.49 ± 0.798
3.253LeuMet: 3.253 ± 0.511
4.88LeuAsn: 4.88 ± 0.563
3.965LeuPro: 3.965 ± 0.574
3.253LeuGln: 3.253 ± 0.63
3.457LeuArg: 3.457 ± 0.653
6.913LeuSer: 6.913 ± 0.991
4.473LeuThr: 4.473 ± 0.789
4.168LeuVal: 4.168 ± 0.626
0.61LeuTrp: 0.61 ± 0.254
3.05LeuTyr: 3.05 ± 0.451
0.0LeuXaa: 0.0 ± 0.0
Met
2.135MetAla: 2.135 ± 0.469
0.203MetCys: 0.203 ± 0.124
0.712MetAsp: 0.712 ± 0.26
1.728MetGlu: 1.728 ± 0.427
0.61MetPhe: 0.61 ± 0.227
1.525MetGly: 1.525 ± 0.39
0.61MetHis: 0.61 ± 0.278
2.643MetIle: 2.643 ± 0.546
2.135MetLys: 2.135 ± 0.512
2.338MetLeu: 2.338 ± 0.52
0.508MetMet: 0.508 ± 0.25
2.135MetAsn: 2.135 ± 0.384
0.915MetPro: 0.915 ± 0.269
1.932MetGln: 1.932 ± 0.489
1.322MetArg: 1.322 ± 0.387
1.932MetSer: 1.932 ± 0.516
2.44MetThr: 2.44 ± 0.564
1.22MetVal: 1.22 ± 0.375
0.203MetTrp: 0.203 ± 0.131
0.508MetTyr: 0.508 ± 0.211
0.0MetXaa: 0.0 ± 0.0
Asn
5.795AsnAla: 5.795 ± 0.679
0.508AsnCys: 0.508 ± 0.222
3.558AsnAsp: 3.558 ± 0.711
4.067AsnGlu: 4.067 ± 0.518
1.525AsnPhe: 1.525 ± 0.344
4.372AsnGly: 4.372 ± 0.668
0.813AsnHis: 0.813 ± 0.334
3.152AsnIle: 3.152 ± 0.651
3.863AsnLys: 3.863 ± 0.649
5.083AsnLeu: 5.083 ± 0.788
1.118AsnMet: 1.118 ± 0.323
3.05AsnAsn: 3.05 ± 0.368
2.237AsnPro: 2.237 ± 0.43
2.542AsnGln: 2.542 ± 0.767
1.932AsnArg: 1.932 ± 0.437
3.05AsnSer: 3.05 ± 0.508
2.948AsnThr: 2.948 ± 0.514
2.033AsnVal: 2.033 ± 0.36
1.22AsnTrp: 1.22 ± 0.403
1.83AsnTyr: 1.83 ± 0.4
0.0AsnXaa: 0.0 ± 0.0
Pro
1.728ProAla: 1.728 ± 0.366
0.102ProCys: 0.102 ± 0.099
2.44ProAsp: 2.44 ± 0.601
1.83ProGlu: 1.83 ± 0.45
1.83ProPhe: 1.83 ± 0.551
0.712ProGly: 0.712 ± 0.263
0.203ProHis: 0.203 ± 0.121
1.728ProIle: 1.728 ± 0.436
2.745ProLys: 2.745 ± 0.526
2.643ProLeu: 2.643 ± 0.492
0.712ProMet: 0.712 ± 0.274
2.237ProAsn: 2.237 ± 0.536
0.915ProPro: 0.915 ± 0.311
2.237ProGln: 2.237 ± 0.813
1.525ProArg: 1.525 ± 0.452
2.338ProSer: 2.338 ± 0.442
1.728ProThr: 1.728 ± 0.386
3.355ProVal: 3.355 ± 0.606
0.407ProTrp: 0.407 ± 0.216
1.118ProTyr: 1.118 ± 0.324
0.0ProXaa: 0.0 ± 0.0
Gln
4.677GlnAla: 4.677 ± 0.735
0.203GlnCys: 0.203 ± 0.16
2.643GlnAsp: 2.643 ± 0.616
1.83GlnGlu: 1.83 ± 0.382
2.135GlnPhe: 2.135 ± 0.507
2.338GlnGly: 2.338 ± 0.674
1.118GlnHis: 1.118 ± 0.396
2.948GlnIle: 2.948 ± 0.623
2.135GlnLys: 2.135 ± 0.395
3.253GlnLeu: 3.253 ± 0.596
1.118GlnMet: 1.118 ± 0.328
2.643GlnAsn: 2.643 ± 0.656
1.525GlnPro: 1.525 ± 0.394
1.728GlnGln: 1.728 ± 0.671
1.627GlnArg: 1.627 ± 0.396
3.355GlnSer: 3.355 ± 0.665
1.83GlnThr: 1.83 ± 0.432
2.745GlnVal: 2.745 ± 0.413
0.203GlnTrp: 0.203 ± 0.144
1.22GlnTyr: 1.22 ± 0.319
0.0GlnXaa: 0.0 ± 0.0
Arg
2.338ArgAla: 2.338 ± 0.521
0.712ArgCys: 0.712 ± 0.318
2.847ArgAsp: 2.847 ± 0.531
4.778ArgGlu: 4.778 ± 1.144
1.932ArgPhe: 1.932 ± 0.443
2.033ArgGly: 2.033 ± 0.467
0.712ArgHis: 0.712 ± 0.309
2.847ArgIle: 2.847 ± 0.523
3.253ArgLys: 3.253 ± 0.78
3.66ArgLeu: 3.66 ± 0.74
0.712ArgMet: 0.712 ± 0.3
1.627ArgAsn: 1.627 ± 0.401
0.915ArgPro: 0.915 ± 0.42
2.44ArgGln: 2.44 ± 0.461
2.033ArgArg: 2.033 ± 0.445
3.05ArgSer: 3.05 ± 0.458
1.423ArgThr: 1.423 ± 0.437
3.965ArgVal: 3.965 ± 0.651
0.712ArgTrp: 0.712 ± 0.263
1.83ArgTyr: 1.83 ± 0.375
0.0ArgXaa: 0.0 ± 0.0
Ser
4.982SerAla: 4.982 ± 0.761
0.915SerCys: 0.915 ± 0.279
4.677SerAsp: 4.677 ± 0.662
5.287SerGlu: 5.287 ± 0.66
2.643SerPhe: 2.643 ± 0.501
5.592SerGly: 5.592 ± 0.846
0.712SerHis: 0.712 ± 0.293
4.575SerIle: 4.575 ± 0.705
4.677SerLys: 4.677 ± 0.685
4.982SerLeu: 4.982 ± 0.887
1.728SerMet: 1.728 ± 0.411
3.457SerAsn: 3.457 ± 0.596
2.847SerPro: 2.847 ± 0.542
4.067SerGln: 4.067 ± 0.592
3.05SerArg: 3.05 ± 0.459
5.083SerSer: 5.083 ± 1.252
2.338SerThr: 2.338 ± 0.479
3.457SerVal: 3.457 ± 0.55
0.508SerTrp: 0.508 ± 0.231
1.627SerTyr: 1.627 ± 0.331
0.0SerXaa: 0.0 ± 0.0
Thr
5.185ThrAla: 5.185 ± 0.828
0.915ThrCys: 0.915 ± 0.316
3.152ThrAsp: 3.152 ± 0.583
4.372ThrGlu: 4.372 ± 0.546
2.745ThrPhe: 2.745 ± 0.552
4.473ThrGly: 4.473 ± 0.787
0.712ThrHis: 0.712 ± 0.266
2.542ThrIle: 2.542 ± 0.473
4.27ThrLys: 4.27 ± 0.652
3.05ThrLeu: 3.05 ± 0.631
0.915ThrMet: 0.915 ± 0.289
3.66ThrAsn: 3.66 ± 0.673
1.932ThrPro: 1.932 ± 0.402
2.237ThrGln: 2.237 ± 0.462
1.627ThrArg: 1.627 ± 0.428
3.05ThrSer: 3.05 ± 0.614
2.847ThrThr: 2.847 ± 0.491
5.185ThrVal: 5.185 ± 0.927
0.508ThrTrp: 0.508 ± 0.219
2.643ThrTyr: 2.643 ± 0.491
0.0ThrXaa: 0.0 ± 0.0
Val
6.1ValAla: 6.1 ± 0.809
0.915ValCys: 0.915 ± 0.324
4.778ValAsp: 4.778 ± 0.675
3.152ValGlu: 3.152 ± 0.499
2.948ValPhe: 2.948 ± 0.603
4.067ValGly: 4.067 ± 0.614
0.712ValHis: 0.712 ± 0.275
2.847ValIle: 2.847 ± 0.446
5.287ValLys: 5.287 ± 0.749
4.372ValLeu: 4.372 ± 0.752
1.627ValMet: 1.627 ± 0.449
3.558ValAsn: 3.558 ± 0.574
2.237ValPro: 2.237 ± 0.648
1.017ValGln: 1.017 ± 0.343
2.847ValArg: 2.847 ± 0.485
3.965ValSer: 3.965 ± 0.748
3.863ValThr: 3.863 ± 0.672
4.067ValVal: 4.067 ± 0.64
0.305ValTrp: 0.305 ± 0.188
2.033ValTyr: 2.033 ± 0.417
0.0ValXaa: 0.0 ± 0.0
Trp
0.915TrpAla: 0.915 ± 0.355
0.102TrpCys: 0.102 ± 0.095
0.813TrpAsp: 0.813 ± 0.333
0.61TrpGlu: 0.61 ± 0.247
0.61TrpPhe: 0.61 ± 0.301
0.712TrpGly: 0.712 ± 0.285
0.305TrpHis: 0.305 ± 0.146
0.813TrpIle: 0.813 ± 0.303
0.508TrpLys: 0.508 ± 0.214
1.118TrpLeu: 1.118 ± 0.309
0.305TrpMet: 0.305 ± 0.172
0.61TrpAsn: 0.61 ± 0.249
0.712TrpPro: 0.712 ± 0.265
0.61TrpGln: 0.61 ± 0.303
0.813TrpArg: 0.813 ± 0.313
0.712TrpSer: 0.712 ± 0.225
0.508TrpThr: 0.508 ± 0.249
0.712TrpVal: 0.712 ± 0.287
0.203TrpTrp: 0.203 ± 0.159
0.305TrpTyr: 0.305 ± 0.188
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.457TyrAla: 3.457 ± 0.603
0.305TyrCys: 0.305 ± 0.21
1.322TyrAsp: 1.322 ± 0.439
2.847TyrGlu: 2.847 ± 0.437
1.22TyrPhe: 1.22 ± 0.361
2.745TyrGly: 2.745 ± 0.445
0.508TyrHis: 0.508 ± 0.203
2.033TyrIle: 2.033 ± 0.494
2.44TyrLys: 2.44 ± 0.576
2.542TyrLeu: 2.542 ± 0.379
0.61TyrMet: 0.61 ± 0.215
2.135TyrAsn: 2.135 ± 0.381
1.423TyrPro: 1.423 ± 0.447
1.423TyrGln: 1.423 ± 0.358
1.627TyrArg: 1.627 ± 0.347
2.135TyrSer: 2.135 ± 0.432
3.152TyrThr: 3.152 ± 0.568
2.135TyrVal: 2.135 ± 0.473
0.61TyrTrp: 0.61 ± 0.227
0.915TyrTyr: 0.915 ± 0.287
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 58 proteins (9837 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski