Lactobacillus phage 3-SAC12

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 61 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Y5FFV5|A0A4Y5FFV5_9CAUD Tail protein OS=Lactobacillus phage 3-SAC12 OX=2510940 GN=A3SAC12_0019 PE=4 SV=1
MM1 pKa = 7.14YY2 pKa = 7.89RR3 pKa = 11.84TTFDD7 pKa = 3.29FDD9 pKa = 3.73KK10 pKa = 11.11SEE12 pKa = 4.12KK13 pKa = 10.72DD14 pKa = 3.3SVFNLEE20 pKa = 4.25FSGSEE25 pKa = 3.9YY26 pKa = 10.68EE27 pKa = 5.85FEE29 pKa = 3.73ISYY32 pKa = 11.34NKK34 pKa = 10.09VNDD37 pKa = 3.34SFYY40 pKa = 10.32ITIRR44 pKa = 11.84DD45 pKa = 3.66VNGEE49 pKa = 4.4DD50 pKa = 3.49IVTCEE55 pKa = 4.06KK56 pKa = 10.2IVYY59 pKa = 9.31GEE61 pKa = 5.0KK62 pKa = 10.09IFADD66 pKa = 3.7INFGDD71 pKa = 4.11LPEE74 pKa = 6.54DD75 pKa = 3.66DD76 pKa = 5.76FVPLDD81 pKa = 3.83EE82 pKa = 5.07SGKK85 pKa = 8.25CHH87 pKa = 6.37SVTFEE92 pKa = 3.83TMNDD96 pKa = 3.34GVYY99 pKa = 8.9ITVDD103 pKa = 3.37DD104 pKa = 4.9LFTGEE109 pKa = 4.65LDD111 pKa = 3.91EE112 pKa = 6.13NNTQSGEE119 pKa = 4.09LNPDD123 pKa = 3.56GNQSEE128 pKa = 4.97MIADD132 pKa = 4.64DD133 pKa = 4.46NDD135 pKa = 4.28SDD137 pKa = 6.29DD138 pKa = 4.7SDD140 pKa = 3.52EE141 pKa = 5.49DD142 pKa = 4.04YY143 pKa = 11.78

Molecular weight: 16.31 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Y5FFV4|A0A4Y5FFV4_9CAUD Uncharacterized protein OS=Lactobacillus phage 3-SAC12 OX=2510940 GN=A3SAC12_0018 PE=4 SV=1
MM1 pKa = 7.78VSEE4 pKa = 4.67SEE6 pKa = 4.16LLKK9 pKa = 10.71KK10 pKa = 10.47LRR12 pKa = 11.84LEE14 pKa = 3.87RR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84LKK19 pKa = 10.75SLNKK23 pKa = 8.92YY24 pKa = 7.37QAEE27 pKa = 3.96AGALKK32 pKa = 10.12KK33 pKa = 10.86VPEE36 pKa = 4.29HH37 pKa = 7.17SEE39 pKa = 3.79EE40 pKa = 4.03EE41 pKa = 3.62LRR43 pKa = 11.84MIVRR47 pKa = 11.84ANEE50 pKa = 4.09FGTMTIPARR59 pKa = 11.84SFLRR63 pKa = 11.84GAAARR68 pKa = 11.84NKK70 pKa = 8.75RR71 pKa = 11.84HH72 pKa = 5.2AWRR75 pKa = 11.84YY76 pKa = 7.03AASVSVGKK84 pKa = 10.32IINGEE89 pKa = 4.04LTAMGAYY96 pKa = 9.6KK97 pKa = 10.46SISKK101 pKa = 10.24VMAEE105 pKa = 4.33GVRR108 pKa = 11.84HH109 pKa = 5.82QISIVGPANAPATIKK124 pKa = 10.7KK125 pKa = 9.9KK126 pKa = 10.55GRR128 pKa = 11.84NEE130 pKa = 3.93PLVDD134 pKa = 3.09TGGLRR139 pKa = 11.84RR140 pKa = 11.84SIDD143 pKa = 3.16GRR145 pKa = 11.84VTTRR149 pKa = 11.84GG150 pKa = 3.21

Molecular weight: 16.57 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

61

0

61

12764

48

1673

209.2

23.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.302 ± 0.499

0.642 ± 0.122

6.338 ± 0.407

6.158 ± 0.586

3.596 ± 0.203

6.808 ± 0.761

1.645 ± 0.132

6.847 ± 0.341

8.877 ± 0.491

7.129 ± 0.253

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.766 ± 0.164

6.84 ± 0.303

2.954 ± 0.216

3.345 ± 0.203

3.823 ± 0.232

7.067 ± 0.352

6.127 ± 0.354

6.526 ± 0.265

1.097 ± 0.126

4.113 ± 0.331
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski