Pyrobaculum spherical virus (isolate United States/Yellowstone) (PSV)

Taxonomy: Viruses; Globuloviridae; Alphaglobulovirus; Alphaglobulovirus PSV; Pyrobaculum spherical virus

Average proteome isoelectric point is 7.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q6ZYJ3|Q6ZYJ3_PSVY Uncharacterized protein OS=Pyrobaculum spherical virus (isolate United States/Yellowstone) OX=654907 PE=4 SV=1
MM1 pKa = 7.64SAFDD5 pKa = 3.95EE6 pKa = 4.68FNEE9 pKa = 4.47GFGLDD14 pKa = 3.49VSDD17 pKa = 4.38TPEE20 pKa = 3.66EE21 pKa = 3.93LAFEE25 pKa = 4.34TEE27 pKa = 4.26SAIEE31 pKa = 4.33EE32 pKa = 4.46IEE34 pKa = 4.47SEE36 pKa = 4.5TSPGDD41 pKa = 3.46QPKK44 pKa = 10.48GSEE47 pKa = 4.08PEE49 pKa = 4.29EE50 pKa = 3.62IRR52 pKa = 11.84VWAEE56 pKa = 3.41EE57 pKa = 4.06KK58 pKa = 10.43ARR60 pKa = 11.84KK61 pKa = 9.06AVEE64 pKa = 3.91EE65 pKa = 4.21GRR67 pKa = 11.84EE68 pKa = 3.99VTNWADD74 pKa = 3.35WIMGWRR80 pKa = 11.84TPNASEE86 pKa = 3.98KK87 pKa = 10.83KK88 pKa = 8.8MEE90 pKa = 3.85FMYY93 pKa = 9.74WYY95 pKa = 8.7TRR97 pKa = 11.84TYY99 pKa = 10.85LEE101 pKa = 3.94EE102 pKa = 5.17AKK104 pKa = 10.56DD105 pKa = 3.82IRR107 pKa = 11.84PDD109 pKa = 3.21IADD112 pKa = 3.56ALARR116 pKa = 11.84GMAGLAFGRR125 pKa = 11.84TDD127 pKa = 2.93WVASMLDD134 pKa = 3.7PQIMRR139 pKa = 11.84HH140 pKa = 6.16IYY142 pKa = 9.49TDD144 pKa = 3.55PEE146 pKa = 3.88VARR149 pKa = 11.84IYY151 pKa = 11.08SEE153 pKa = 3.87TRR155 pKa = 11.84DD156 pKa = 3.4MLRR159 pKa = 11.84RR160 pKa = 11.84VSDD163 pKa = 3.8YY164 pKa = 11.03YY165 pKa = 10.57ISLTTMEE172 pKa = 4.87LGKK175 pKa = 10.63VADD178 pKa = 5.08IIAEE182 pKa = 4.05AKK184 pKa = 10.59AKK186 pKa = 10.62GEE188 pKa = 3.98NPEE191 pKa = 3.92VVARR195 pKa = 11.84EE196 pKa = 3.66IAEE199 pKa = 4.12AVPRR203 pKa = 11.84LSPKK207 pKa = 10.13SLYY210 pKa = 10.53FNLYY214 pKa = 9.81YY215 pKa = 10.04IGRR218 pKa = 11.84SIGDD222 pKa = 3.44NYY224 pKa = 10.02VLEE227 pKa = 4.4VARR230 pKa = 11.84VLSKK234 pKa = 9.98MRR236 pKa = 11.84RR237 pKa = 11.84RR238 pKa = 3.76

Molecular weight: 27.32 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q6ZYF6|Q6ZYF6_PSVY Uncharacterized protein OS=Pyrobaculum spherical virus (isolate United States/Yellowstone) OX=654907 PE=1 SV=1
MM1 pKa = 7.21PRR3 pKa = 11.84KK4 pKa = 9.5MSDD7 pKa = 2.88TMHH10 pKa = 5.85TRR12 pKa = 11.84MIKK15 pKa = 7.32TLRR18 pKa = 11.84YY19 pKa = 8.27MITEE23 pKa = 4.76APCTTIARR31 pKa = 11.84VANKK35 pKa = 10.31LDD37 pKa = 3.97VSWSMARR44 pKa = 11.84NALYY48 pKa = 10.32QLAKK52 pKa = 10.69NKK54 pKa = 9.16MVLKK58 pKa = 10.6VEE60 pKa = 4.5ISRR63 pKa = 11.84ATIWCVDD70 pKa = 3.26EE71 pKa = 4.84NAATNAIFEE80 pKa = 4.65IKK82 pKa = 8.82RR83 pKa = 11.84TLWRR87 pKa = 11.84LICNSRR93 pKa = 11.84RR94 pKa = 11.84RR95 pKa = 11.84YY96 pKa = 7.93ITPSDD101 pKa = 3.8MLRR104 pKa = 11.84LIAADD109 pKa = 3.91TEE111 pKa = 4.17AHH113 pKa = 6.46NIFAKK118 pKa = 10.68YY119 pKa = 10.23VDD121 pKa = 3.76VNSTHH126 pKa = 5.72GHH128 pKa = 4.75TLHH131 pKa = 7.09FIASALEE138 pKa = 4.1DD139 pKa = 3.96LLGKK143 pKa = 9.87PMDD146 pKa = 3.4KK147 pKa = 10.22RR148 pKa = 11.84QRR150 pKa = 11.84RR151 pKa = 11.84LLFHH155 pKa = 5.46VTPRR159 pKa = 11.84FCKK162 pKa = 10.11KK163 pKa = 10.52APTLADD169 pKa = 3.31INLSHH174 pKa = 6.09YY175 pKa = 8.63TSAYY179 pKa = 9.68RR180 pKa = 11.84LVTFKK185 pKa = 11.22VPTAMHH191 pKa = 6.82NDD193 pKa = 3.2MLEE196 pKa = 3.98AARR199 pKa = 11.84TLGLTTSEE207 pKa = 4.53LVTMAIDD214 pKa = 3.58RR215 pKa = 11.84LLSQYY220 pKa = 10.91RR221 pKa = 11.84HH222 pKa = 5.52VLDD225 pKa = 4.36GKK227 pKa = 11.06GGADD231 pKa = 3.85LKK233 pKa = 11.63ANN235 pKa = 3.78

Molecular weight: 26.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

48

0

48

8428

61

581

175.6

19.61

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.792 ± 0.469

1.009 ± 0.161

4.153 ± 0.302

5.019 ± 0.433

4.117 ± 0.301

7.202 ± 0.42

1.661 ± 0.159

6.739 ± 0.261

5.233 ± 0.338

10.168 ± 0.535

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.919 ± 0.169

3.429 ± 0.18

4.224 ± 0.293

2.219 ± 0.21

5.327 ± 0.361

5.256 ± 0.328

5.672 ± 0.418

9.955 ± 0.367

1.614 ± 0.168

5.244 ± 0.346
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski