Amino acid dipepetide frequency for Sphingomonas sp. Leaf17

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	21.504AlaAla: 21.504 ± 0.231	1.011AlaCys: 1.011 ± 0.035	8.762AlaAsp: 8.762 ± 0.116	6.553AlaGlu: 6.553 ± 0.107	4.483AlaPhe: 4.483 ± 0.067	12.812AlaGly: 12.812 ± 0.116	2.363AlaHis: 2.363 ± 0.055	7.49AlaIle: 7.49 ± 0.078	3.836AlaLys: 3.836 ± 0.077	14.129AlaLeu: 14.129 ± 0.156	4.431AlaMet: 4.431 ± 0.075	3.149AlaAsn: 3.149 ± 0.066	6.647AlaPro: 6.647 ± 0.098	4.514AlaGln: 4.514 ± 0.078	10.969AlaArg: 10.969 ± 0.146	6.362AlaSer: 6.362 ± 0.08	8.429AlaThr: 8.429 ± 0.109	9.79AlaVal: 9.79 ± 0.124	1.703AlaTrp: 1.703 ± 0.046	2.684AlaTyr: 2.684 ± 0.052	0.0AlaXaa: 0.0 ± 0.0
Cys	0.88CysAla: 0.88 ± 0.034	0.065CysCys: 0.065 ± 0.009	0.513CysAsp: 0.513 ± 0.024	0.299CysGlu: 0.299 ± 0.019	0.26CysPhe: 0.26 ± 0.018	0.813CysGly: 0.813 ± 0.036	0.153CysHis: 0.153 ± 0.014	0.328CysIle: 0.328 ± 0.018	0.136CysLys: 0.136 ± 0.012	0.585CysLeu: 0.585 ± 0.028	0.136CysMet: 0.136 ± 0.012	0.171CysAsn: 0.171 ± 0.014	0.411CysPro: 0.411 ± 0.02	0.16CysGln: 0.16 ± 0.013	0.507CysArg: 0.507 ± 0.025	0.352CysSer: 0.352 ± 0.02	0.362CysThr: 0.362 ± 0.017	0.534CysVal: 0.534 ± 0.024	0.095CysTrp: 0.095 ± 0.01	0.143CysTyr: 0.143 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	9.05AspAla: 9.05 ± 0.106	0.442AspCys: 0.442 ± 0.023	3.745AspAsp: 3.745 ± 0.071	2.912AspGlu: 2.912 ± 0.053	2.07AspPhe: 2.07 ± 0.043	6.174AspGly: 6.174 ± 0.084	1.403AspHis: 1.403 ± 0.035	2.944AspIle: 2.944 ± 0.056	1.461AspLys: 1.461 ± 0.045	6.07AspLeu: 6.07 ± 0.087	1.48AspMet: 1.48 ± 0.046	1.247AspAsn: 1.247 ± 0.04	4.0AspPro: 4.0 ± 0.076	1.823AspGln: 1.823 ± 0.045	5.627AspArg: 5.627 ± 0.09	2.375AspSer: 2.375 ± 0.05	3.453AspThr: 3.453 ± 0.072	4.467AspVal: 4.467 ± 0.063	1.314AspTrp: 1.314 ± 0.036	1.57AspTyr: 1.57 ± 0.041	0.0AspXaa: 0.0 ± 0.0
Glu	6.495GluAla: 6.495 ± 0.116	0.25GluCys: 0.25 ± 0.016	2.441GluAsp: 2.441 ± 0.061	1.988GluGlu: 1.988 ± 0.06	1.1GluPhe: 1.1 ± 0.034	3.895GluGly: 3.895 ± 0.062	0.913GluHis: 0.913 ± 0.032	2.37GluIle: 2.37 ± 0.056	1.501GluLys: 1.501 ± 0.05	3.812GluLeu: 3.812 ± 0.066	1.206GluMet: 1.206 ± 0.04	1.068GluAsn: 1.068 ± 0.036	2.233GluPro: 2.233 ± 0.056	1.697GluGln: 1.697 ± 0.047	4.274GluArg: 4.274 ± 0.077	1.93GluSer: 1.93 ± 0.052	3.089GluThr: 3.089 ± 0.059	2.971GluVal: 2.971 ± 0.056	0.602GluTrp: 0.602 ± 0.024	0.881GluTyr: 0.881 ± 0.033	0.0GluXaa: 0.0 ± 0.0
Phe	5.137PheAla: 5.137 ± 0.073	0.251PheCys: 0.251 ± 0.017	2.694PheAsp: 2.694 ± 0.05	1.627PheGlu: 1.627 ± 0.046	1.092PhePhe: 1.092 ± 0.041	3.592PheGly: 3.592 ± 0.072	0.657PheHis: 0.657 ± 0.028	1.251PheIle: 1.251 ± 0.046	0.714PheLys: 0.714 ± 0.031	2.976PheLeu: 2.976 ± 0.06	0.657PheMet: 0.657 ± 0.03	0.894PheAsn: 0.894 ± 0.035	1.379PhePro: 1.379 ± 0.037	0.803PheGln: 0.803 ± 0.033	2.204PheArg: 2.204 ± 0.052	1.756PheSer: 1.756 ± 0.049	2.108PheThr: 2.108 ± 0.043	2.677PheVal: 2.677 ± 0.053	0.469PheTrp: 0.469 ± 0.025	0.84PheTyr: 0.84 ± 0.03	0.0PheXaa: 0.0 ± 0.0
Gly	10.487GlyAla: 10.487 ± 0.105	0.812GlyCys: 0.812 ± 0.031	5.512GlyAsp: 5.512 ± 0.069	4.134GlyGlu: 4.134 ± 0.066	3.645GlyPhe: 3.645 ± 0.06	8.755GlyGly: 8.755 ± 0.118	1.905GlyHis: 1.905 ± 0.048	4.757GlyIle: 4.757 ± 0.073	2.892GlyLys: 2.892 ± 0.054	8.905GlyLeu: 8.905 ± 0.102	2.418GlyMet: 2.418 ± 0.049	2.197GlyAsn: 2.197 ± 0.064	3.703GlyPro: 3.703 ± 0.072	2.952GlyGln: 2.952 ± 0.055	7.158GlyArg: 7.158 ± 0.089	4.605GlySer: 4.605 ± 0.076	5.46GlyThr: 5.46 ± 0.09	6.933GlyVal: 6.933 ± 0.09	1.706GlyTrp: 1.706 ± 0.042	2.433GlyTyr: 2.433 ± 0.054	0.002GlyXaa: 0.002 ± 0.002
His	2.547HisAla: 2.547 ± 0.064	0.162HisCys: 0.162 ± 0.014	1.361HisAsp: 1.361 ± 0.042	0.852HisGlu: 0.852 ± 0.031	0.682HisPhe: 0.682 ± 0.023	1.94HisGly: 1.94 ± 0.041	0.539HisHis: 0.539 ± 0.026	0.829HisIle: 0.829 ± 0.028	0.344HisLys: 0.344 ± 0.019	1.779HisLeu: 1.779 ± 0.042	0.42HisMet: 0.42 ± 0.019	0.389HisAsn: 0.389 ± 0.02	1.258HisPro: 1.258 ± 0.038	0.471HisGln: 0.471 ± 0.022	1.485HisArg: 1.485 ± 0.04	0.716HisSer: 0.716 ± 0.028	0.735HisThr: 0.735 ± 0.027	1.457HisVal: 1.457 ± 0.038	0.335HisTrp: 0.335 ± 0.018	0.469HisTyr: 0.469 ± 0.022	0.0HisXaa: 0.0 ± 0.0
Ile	8.761IleAla: 8.761 ± 0.109	0.375IleCys: 0.375 ± 0.023	4.181IleAsp: 4.181 ± 0.076	3.038IleGlu: 3.038 ± 0.06	1.364IlePhe: 1.364 ± 0.036	5.4IleGly: 5.4 ± 0.09	0.842IleHis: 0.842 ± 0.031	1.739IleIle: 1.739 ± 0.051	0.989IleLys: 0.989 ± 0.037	4.05IleLeu: 4.05 ± 0.065	0.782IleMet: 0.782 ± 0.037	1.163IleAsn: 1.163 ± 0.038	2.186IlePro: 2.186 ± 0.05	1.044IleGln: 1.044 ± 0.033	3.258IleArg: 3.258 ± 0.06	1.987IleSer: 1.987 ± 0.053	2.57IleThr: 2.57 ± 0.054	4.68IleVal: 4.68 ± 0.066	0.534IleTrp: 0.534 ± 0.026	0.877IleTyr: 0.877 ± 0.032	0.0IleXaa: 0.0 ± 0.0
Lys	3.818LysAla: 3.818 ± 0.074	0.11LysCys: 0.11 ± 0.012	1.362LysAsp: 1.362 ± 0.04	0.842LysGlu: 0.842 ± 0.034	0.681LysPhe: 0.681 ± 0.03	2.319LysGly: 2.319 ± 0.056	0.396LysHis: 0.396 ± 0.021	1.196LysIle: 1.196 ± 0.038	0.908LysLys: 0.908 ± 0.04	2.691LysLeu: 2.691 ± 0.069	0.694LysMet: 0.694 ± 0.031	0.591LysAsn: 0.591 ± 0.028	1.881LysPro: 1.881 ± 0.054	0.735LysGln: 0.735 ± 0.03	2.071LysArg: 2.071 ± 0.046	1.331LysSer: 1.331 ± 0.042	1.756LysThr: 1.756 ± 0.048	1.961LysVal: 1.961 ± 0.05	0.318LysTrp: 0.318 ± 0.019	0.56LysTyr: 0.56 ± 0.025	0.0LysXaa: 0.0 ± 0.0
Leu	14.357LeuAla: 14.357 ± 0.132	0.648LeuCys: 0.648 ± 0.025	6.255LeuAsp: 6.255 ± 0.096	3.862LeuGlu: 3.862 ± 0.072	3.49LeuPhe: 3.49 ± 0.071	8.043LeuGly: 8.043 ± 0.103	1.748LeuHis: 1.748 ± 0.047	4.545LeuIle: 4.545 ± 0.084	2.719LeuLys: 2.719 ± 0.065	8.711LeuLeu: 8.711 ± 0.132	2.185LeuMet: 2.185 ± 0.051	2.25LeuAsn: 2.25 ± 0.054	5.562LeuPro: 5.562 ± 0.079	2.154LeuGln: 2.154 ± 0.053	6.638LeuArg: 6.638 ± 0.088	5.613LeuSer: 5.613 ± 0.093	5.978LeuThr: 5.978 ± 0.084	7.635LeuVal: 7.635 ± 0.106	1.241LeuTrp: 1.241 ± 0.045	1.977LeuTyr: 1.977 ± 0.049	0.002LeuXaa: 0.002 ± 0.002
Met	3.636MetAla: 3.636 ± 0.064	0.135MetCys: 0.135 ± 0.013	1.127MetAsp: 1.127 ± 0.036	0.844MetGlu: 0.844 ± 0.03	0.645MetPhe: 0.645 ± 0.031	1.901MetGly: 1.901 ± 0.047	0.357MetHis: 0.357 ± 0.02	1.454MetIle: 1.454 ± 0.044	0.902MetLys: 0.902 ± 0.034	2.714MetLeu: 2.714 ± 0.051	0.676MetMet: 0.676 ± 0.03	0.63MetAsn: 0.63 ± 0.028	1.612MetPro: 1.612 ± 0.046	0.696MetGln: 0.696 ± 0.027	1.838MetArg: 1.838 ± 0.037	1.263MetSer: 1.263 ± 0.035	2.181MetThr: 2.181 ± 0.046	1.741MetVal: 1.741 ± 0.049	0.217MetTrp: 0.217 ± 0.016	0.287MetTyr: 0.287 ± 0.019	0.0MetXaa: 0.0 ± 0.0
Asn	3.26AsnAla: 3.26 ± 0.061	0.199AsnCys: 0.199 ± 0.015	1.458AsnAsp: 1.458 ± 0.041	0.895AsnGlu: 0.895 ± 0.029	0.835AsnPhe: 0.835 ± 0.035	2.378AsnGly: 2.378 ± 0.062	0.448AsnHis: 0.448 ± 0.023	1.149AsnIle: 1.149 ± 0.038	0.507AsnLys: 0.507 ± 0.024	2.189AsnLeu: 2.189 ± 0.052	0.472AsnMet: 0.472 ± 0.021	0.595AsnAsn: 0.595 ± 0.03	1.679AsnPro: 1.679 ± 0.052	0.619AsnGln: 0.619 ± 0.027	1.809AsnArg: 1.809 ± 0.046	0.998AsnSer: 0.998 ± 0.038	1.214AsnThr: 1.214 ± 0.047	1.74AsnVal: 1.74 ± 0.049	0.366AsnTrp: 0.366 ± 0.021	0.646AsnTyr: 0.646 ± 0.028	0.0AsnXaa: 0.0 ± 0.0
Pro	7.985ProAla: 7.985 ± 0.144	0.304ProCys: 0.304 ± 0.019	4.081ProAsp: 4.081 ± 0.067	2.742ProGlu: 2.742 ± 0.061	1.869ProPhe: 1.869 ± 0.042	4.93ProGly: 4.93 ± 0.073	0.99ProHis: 0.99 ± 0.031	2.485ProIle: 2.485 ± 0.053	1.431ProLys: 1.431 ± 0.039	4.668ProLeu: 4.668 ± 0.068	1.36ProMet: 1.36 ± 0.045	1.203ProAsn: 1.203 ± 0.036	2.88ProPro: 2.88 ± 0.079	1.532ProGln: 1.532 ± 0.043	3.166ProArg: 3.166 ± 0.059	2.459ProSer: 2.459 ± 0.056	3.134ProThr: 3.134 ± 0.062	5.086ProVal: 5.086 ± 0.084	0.685ProTrp: 0.685 ± 0.026	1.106ProTyr: 1.106 ± 0.029	0.0ProXaa: 0.0 ± 0.0
Gln	4.159GlnAla: 4.159 ± 0.077	0.182GlnCys: 0.182 ± 0.013	1.379GlnAsp: 1.379 ± 0.037	0.955GlnGlu: 0.955 ± 0.038	0.986GlnPhe: 0.986 ± 0.032	2.335GlnGly: 2.335 ± 0.051	0.478GlnHis: 0.478 ± 0.022	1.532GlnIle: 1.532 ± 0.043	0.738GlnLys: 0.738 ± 0.031	2.634GlnLeu: 2.634 ± 0.049	0.79GlnMet: 0.79 ± 0.026	0.715GlnAsn: 0.715 ± 0.029	1.663GlnPro: 1.663 ± 0.041	1.027GlnGln: 1.027 ± 0.038	2.388GlnArg: 2.388 ± 0.05	1.603GlnSer: 1.603 ± 0.043	1.773GlnThr: 1.773 ± 0.043	2.331GlnVal: 2.331 ± 0.053	0.385GlnTrp: 0.385 ± 0.021	0.603GlnTyr: 0.603 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	9.755ArgAla: 9.755 ± 0.132	0.468ArgCys: 0.468 ± 0.022	4.931ArgAsp: 4.931 ± 0.082	3.366ArgGlu: 3.366 ± 0.075	3.193ArgPhe: 3.193 ± 0.06	5.527ArgGly: 5.527 ± 0.075	1.638ArgHis: 1.638 ± 0.048	4.459ArgIle: 4.459 ± 0.08	1.764ArgLys: 1.764 ± 0.045	8.023ArgLeu: 8.023 ± 0.099	2.146ArgMet: 2.146 ± 0.044	1.724ArgAsn: 1.724 ± 0.044	4.009ArgPro: 4.009 ± 0.085	2.315ArgGln: 2.315 ± 0.052	5.987ArgArg: 5.987 ± 0.102	3.495ArgSer: 3.495 ± 0.059	4.148ArgThr: 4.148 ± 0.075	5.543ArgVal: 5.543 ± 0.088	1.273ArgTrp: 1.273 ± 0.038	1.976ArgTyr: 1.976 ± 0.045	0.0ArgXaa: 0.0 ± 0.0
Ser	6.2SerAla: 6.2 ± 0.085	0.325SerCys: 0.325 ± 0.021	2.988SerAsp: 2.988 ± 0.063	2.005SerGlu: 2.005 ± 0.05	1.872SerPhe: 1.872 ± 0.047	5.126SerGly: 5.126 ± 0.083	0.81SerHis: 0.81 ± 0.028	2.475SerIle: 2.475 ± 0.053	1.188SerLys: 1.188 ± 0.037	4.527SerLeu: 4.527 ± 0.092	1.067SerMet: 1.067 ± 0.035	1.242SerAsn: 1.242 ± 0.04	2.843SerPro: 2.843 ± 0.053	1.27SerGln: 1.27 ± 0.041	3.275SerArg: 3.275 ± 0.063	2.359SerSer: 2.359 ± 0.056	2.648SerThr: 2.648 ± 0.06	3.798SerVal: 3.798 ± 0.066	0.628SerTrp: 0.628 ± 0.026	1.194SerTyr: 1.194 ± 0.041	0.0SerXaa: 0.0 ± 0.0
Thr	7.748ThrAla: 7.748 ± 0.098	0.358ThrCys: 0.358 ± 0.022	3.437ThrAsp: 3.437 ± 0.063	2.259ThrGlu: 2.259 ± 0.047	1.841ThrPhe: 1.841 ± 0.047	6.173ThrGly: 6.173 ± 0.1	0.992ThrHis: 0.992 ± 0.026	3.585ThrIle: 3.585 ± 0.066	1.331ThrLys: 1.331 ± 0.04	6.646ThrLeu: 6.646 ± 0.098	1.441ThrMet: 1.441 ± 0.045	1.366ThrAsn: 1.366 ± 0.046	4.137ThrPro: 4.137 ± 0.07	1.556ThrGln: 1.556 ± 0.041	4.176ThrArg: 4.176 ± 0.063	2.736ThrSer: 2.736 ± 0.061	3.708ThrThr: 3.708 ± 0.078	4.826ThrVal: 4.826 ± 0.085	0.623ThrTrp: 0.623 ± 0.025	1.211ThrTyr: 1.211 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	11.451ValAla: 11.451 ± 0.13	0.496ValCys: 0.496 ± 0.026	4.962ValAsp: 4.962 ± 0.081	4.141ValGlu: 4.141 ± 0.073	2.321ValPhe: 2.321 ± 0.052	6.182ValGly: 6.182 ± 0.096	1.352ValHis: 1.352 ± 0.038	3.693ValIle: 3.693 ± 0.077	1.947ValLys: 1.947 ± 0.048	6.79ValLeu: 6.79 ± 0.086	1.768ValMet: 1.768 ± 0.045	1.865ValAsn: 1.865 ± 0.05	4.247ValPro: 4.247 ± 0.072	2.11ValGln: 2.11 ± 0.049	5.542ValArg: 5.542 ± 0.092	4.027ValSer: 4.027 ± 0.079	5.397ValThr: 5.397 ± 0.084	6.415ValVal: 6.415 ± 0.091	0.907ValTrp: 0.907 ± 0.034	1.381ValTyr: 1.381 ± 0.038	0.0ValXaa: 0.0 ± 0.0
Trp	1.46TrpAla: 1.46 ± 0.04	0.126TrpCys: 0.126 ± 0.011	0.743TrpAsp: 0.743 ± 0.031	0.444TrpGlu: 0.444 ± 0.02	0.556TrpPhe: 0.556 ± 0.025	0.962TrpGly: 0.962 ± 0.033	0.333TrpHis: 0.333 ± 0.016	0.716TrpIle: 0.716 ± 0.028	0.42TrpLys: 0.42 ± 0.022	1.655TrpLeu: 1.655 ± 0.046	0.347TrpMet: 0.347 ± 0.02	0.401TrpAsn: 0.401 ± 0.02	0.748TrpPro: 0.748 ± 0.031	0.528TrpGln: 0.528 ± 0.024	1.389TrpArg: 1.389 ± 0.037	0.837TrpSer: 0.837 ± 0.032	0.935TrpThr: 0.935 ± 0.038	0.823TrpVal: 0.823 ± 0.029	0.276TrpTrp: 0.276 ± 0.017	0.319TrpTyr: 0.319 ± 0.019	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.927TyrAla: 2.927 ± 0.06	0.182TyrCys: 0.182 ± 0.014	1.696TyrAsp: 1.696 ± 0.052	0.959TyrGlu: 0.959 ± 0.033	0.794TyrPhe: 0.794 ± 0.031	2.116TyrGly: 2.116 ± 0.053	0.452TyrHis: 0.452 ± 0.024	0.82TyrIle: 0.82 ± 0.032	0.494TyrLys: 0.494 ± 0.025	2.054TyrLeu: 2.054 ± 0.047	0.383TyrMet: 0.383 ± 0.02	0.595TyrAsn: 0.595 ± 0.03	1.018TyrPro: 1.018 ± 0.034	0.652TyrGln: 0.652 ± 0.024	1.981TyrArg: 1.981 ± 0.048	1.035TyrSer: 1.035 ± 0.035	1.072TyrThr: 1.072 ± 0.032	1.584TyrVal: 1.584 ± 0.043	0.341TyrTrp: 0.341 ± 0.017	0.591TyrTyr: 0.591 ± 0.025	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.002XaaIle: 0.002 ± 0.002	0.0XaaLys: 0.0 ± 0.0	0.002XaaLeu: 0.002 ± 0.002	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2908 proteins (942970 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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