Krasilnikoviella flava

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Promicromonosporaceae; Krasilnikoviella

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4296 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1T5JMS5|A0A1T5JMS5_9MICO Amidohydrolase OS=Krasilnikoviella flava OX=526729 GN=SAMN04324258_1562 PE=4 SV=1
MM1 pKa = 7.78RR2 pKa = 11.84RR3 pKa = 11.84GLRR6 pKa = 11.84RR7 pKa = 11.84AVAASAAGLAGLSLAACAPGGDD29 pKa = 4.32GAPADD34 pKa = 3.79NTSCTNEE41 pKa = 3.88IKK43 pKa = 11.11VPDD46 pKa = 3.93ATRR49 pKa = 11.84VTLWAWYY56 pKa = 8.02PAVEE60 pKa = 4.59QIVDD64 pKa = 3.73TFNAQHH70 pKa = 7.48DD71 pKa = 4.24DD72 pKa = 4.33VQICWTNAGQGADD85 pKa = 3.5EE86 pKa = 4.32YY87 pKa = 11.23GKK89 pKa = 10.87FSTALEE95 pKa = 4.54AGTGAPDD102 pKa = 4.0VIQLEE107 pKa = 4.58SEE109 pKa = 4.0ILPTYY114 pKa = 9.67TILDD118 pKa = 4.17GLTDD122 pKa = 4.57LAPYY126 pKa = 9.98GADD129 pKa = 3.07EE130 pKa = 6.24LEE132 pKa = 4.31DD133 pKa = 3.73QFPAGAWSDD142 pKa = 3.81VSSGDD147 pKa = 3.48AVHH150 pKa = 7.54AIPVDD155 pKa = 3.94GGPVGMLYY163 pKa = 10.37RR164 pKa = 11.84ADD166 pKa = 3.33IFEE169 pKa = 4.74KK170 pKa = 10.95YY171 pKa = 9.94GVEE174 pKa = 5.28PPTTWDD180 pKa = 2.98EE181 pKa = 4.27FARR184 pKa = 11.84AAQDD188 pKa = 4.06LRR190 pKa = 11.84DD191 pKa = 3.79AGYY194 pKa = 10.85DD195 pKa = 3.72GYY197 pKa = 9.45ITNYY201 pKa = 7.9ATNGGAFNYY210 pKa = 10.85ALFAQAGWAPFDD222 pKa = 4.0YY223 pKa = 10.97DD224 pKa = 3.42PAEE227 pKa = 4.5RR228 pKa = 11.84DD229 pKa = 3.32QIGIDD234 pKa = 3.15VDD236 pKa = 3.89TPEE239 pKa = 3.92ARR241 pKa = 11.84QVLSYY246 pKa = 10.59WEE248 pKa = 4.24DD249 pKa = 4.09LIDD252 pKa = 4.55RR253 pKa = 11.84GLVSSDD259 pKa = 3.62DD260 pKa = 4.25AFTSDD265 pKa = 4.0YY266 pKa = 9.53NTSLVDD272 pKa = 3.05GTYY275 pKa = 10.93AVYY278 pKa = 10.06IAAAWGPGYY287 pKa = 10.93LEE289 pKa = 4.69GLSDD293 pKa = 4.54ADD295 pKa = 5.52SGAAWKK301 pKa = 9.5AAPLPQWDD309 pKa = 3.78PAEE312 pKa = 4.16PVQVNQGGSALAVTSQAKK330 pKa = 10.38DD331 pKa = 3.26PEE333 pKa = 4.33AAATVAMEE341 pKa = 4.5LFDD344 pKa = 6.62DD345 pKa = 4.24EE346 pKa = 4.76EE347 pKa = 4.19TWKK350 pKa = 10.68IGVEE354 pKa = 4.08DD355 pKa = 4.36AGLFPLWNLALDD367 pKa = 4.45ADD369 pKa = 4.16WFTDD373 pKa = 2.78RR374 pKa = 11.84RR375 pKa = 11.84YY376 pKa = 10.32PFFGGQQVNRR386 pKa = 11.84DD387 pKa = 3.64VFLDD391 pKa = 3.87AAAQYY396 pKa = 10.53PGFTFSPFQVYY407 pKa = 10.13AYY409 pKa = 10.25DD410 pKa = 3.8RR411 pKa = 11.84QTMALYY417 pKa = 11.17DD418 pKa = 4.0MVEE421 pKa = 4.7GPATAADD428 pKa = 3.77ALATVQDD435 pKa = 3.65SLVRR439 pKa = 11.84YY440 pKa = 9.84AEE442 pKa = 3.97QQGFTVRR449 pKa = 4.02

Molecular weight: 48.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1T5K9A5|A0A1T5K9A5_9MICO ArsR family transcriptional regulator OS=Krasilnikoviella flava OX=526729 GN=SAMN04324258_1958 PE=4 SV=1
MM1 pKa = 7.6KK2 pKa = 10.24VRR4 pKa = 11.84ASLRR8 pKa = 11.84SLKK11 pKa = 10.12RR12 pKa = 11.84LPGAKK17 pKa = 8.89VVRR20 pKa = 11.84RR21 pKa = 11.84RR22 pKa = 11.84GRR24 pKa = 11.84TFVINKK30 pKa = 7.51QNPRR34 pKa = 11.84FKK36 pKa = 10.8ARR38 pKa = 11.84QGG40 pKa = 3.28

Molecular weight: 4.68 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4296

0

4296

1480017

37

2937

344.5

36.53

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.43 ± 0.063

0.506 ± 0.007

6.761 ± 0.035

5.325 ± 0.034

2.65 ± 0.023

9.63 ± 0.034

2.094 ± 0.02

2.705 ± 0.028

1.449 ± 0.023

10.208 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.604 ± 0.015

1.461 ± 0.018

6.017 ± 0.029

2.531 ± 0.02

7.834 ± 0.045

4.837 ± 0.025

6.322 ± 0.035

10.156 ± 0.041

1.604 ± 0.017

1.877 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski