Pseudomonas phage PS-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0H5ARQ8|A0A0H5ARQ8_9CAUD Uncharacterized protein OS=Pseudomonas phage PS-1 OX=1573458 PE=4 SV=1
MM1 pKa = 7.55TEE3 pKa = 4.12QITIAPCPFCEE14 pKa = 5.44GPPCVIAKK22 pKa = 10.32DD23 pKa = 3.79YY24 pKa = 7.43MTGKK28 pKa = 9.72EE29 pKa = 4.14VEE31 pKa = 4.14FDD33 pKa = 3.97RR34 pKa = 11.84PLDD37 pKa = 4.3DD38 pKa = 4.33YY39 pKa = 11.96SEE41 pKa = 4.43EE42 pKa = 4.44SYY44 pKa = 10.85SAHH47 pKa = 5.39VWCHH51 pKa = 4.5EE52 pKa = 4.39CGAQGPEE59 pKa = 3.85VDD61 pKa = 4.12SCTLAIFEE69 pKa = 4.72GLHH72 pKa = 6.76DD73 pKa = 4.31LTVMDD78 pKa = 3.99VARR81 pKa = 11.84IAVEE85 pKa = 3.93RR86 pKa = 11.84WNEE89 pKa = 3.84RR90 pKa = 11.84GNSARR95 pKa = 11.84ACYY98 pKa = 10.32DD99 pKa = 2.95GGAKK103 pKa = 10.33DD104 pKa = 4.19GLNLFPRR111 pKa = 11.84SEE113 pKa = 4.0AA114 pKa = 3.47

Molecular weight: 12.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0H5AUD0|A0A0H5AUD0_9CAUD NinB DNA recombinase OS=Pseudomonas phage PS-1 OX=1573458 PE=4 SV=1
MM1 pKa = 7.47NEE3 pKa = 3.6NAATRR8 pKa = 11.84AAAAAGGQSALARR21 pKa = 11.84LLGCSPQAVQRR32 pKa = 11.84MCATGRR38 pKa = 11.84VPAEE42 pKa = 3.41RR43 pKa = 11.84VLQIEE48 pKa = 4.26AASGVSRR55 pKa = 11.84HH56 pKa = 5.67EE57 pKa = 4.4LRR59 pKa = 11.84PDD61 pKa = 4.15LYY63 pKa = 10.11PKK65 pKa = 9.94SRR67 pKa = 11.84KK68 pKa = 8.99RR69 pKa = 11.84AAAA72 pKa = 3.63

Molecular weight: 7.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

14696

45

1017

186.0

20.48

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.357 ± 0.591

1.341 ± 0.185

5.621 ± 0.211

6.682 ± 0.295

2.953 ± 0.206

7.676 ± 0.273

2.035 ± 0.191

4.797 ± 0.197

4.777 ± 0.304

8.186 ± 0.285

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.599 ± 0.18

3.321 ± 0.19

4.239 ± 0.278

4.266 ± 0.178

7.111 ± 0.308

5.974 ± 0.304

5.369 ± 0.364

6.199 ± 0.234

1.824 ± 0.127

2.674 ± 0.167
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski