Bacillus daliensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Alkalicoccus

Average proteome isoelectric point is 5.96

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3459 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H0GAP2|A0A1H0GAP2_9BACI Multiple sugar transport system permease protein OS=Bacillus daliensis OX=745820 GN=SAMN04488053_10637 PE=3 SV=1
MM1 pKa = 7.54KK2 pKa = 10.1KK3 pKa = 10.31KK4 pKa = 10.52KK5 pKa = 9.77FLSSILGVALVGVIAGCGGSDD26 pKa = 4.55NNDD29 pKa = 2.88NTLNDD34 pKa = 3.64TAEE37 pKa = 4.18GSEE40 pKa = 4.22VNNAGAEE47 pKa = 4.12AEE49 pKa = 4.27EE50 pKa = 4.12EE51 pKa = 4.1AVTIRR56 pKa = 11.84LHH58 pKa = 4.77HH59 pKa = 7.1WYY61 pKa = 10.48NIEE64 pKa = 3.6QDD66 pKa = 2.97NWNEE70 pKa = 3.47VVAAFEE76 pKa = 4.42EE77 pKa = 4.51EE78 pKa = 4.29HH79 pKa = 5.77PHH81 pKa = 7.78INIEE85 pKa = 4.21MNSPEE90 pKa = 4.23NNDD93 pKa = 3.27ANEE96 pKa = 4.37TVQQMDD102 pKa = 4.14LAAASGDD109 pKa = 3.62QLDD112 pKa = 3.86VMMINNAQDD121 pKa = 3.13YY122 pKa = 7.81SQRR125 pKa = 11.84VGQGMFEE132 pKa = 4.21PLNGFIEE139 pKa = 4.42EE140 pKa = 4.61DD141 pKa = 3.71GYY143 pKa = 11.46EE144 pKa = 4.13YY145 pKa = 10.8EE146 pKa = 5.75DD147 pKa = 4.16EE148 pKa = 4.61YY149 pKa = 11.8SVNTAVDD156 pKa = 3.37GDD158 pKa = 4.58YY159 pKa = 11.2YY160 pKa = 11.55ALPGKK165 pKa = 10.47FNGFFVMLNEE175 pKa = 4.98DD176 pKa = 3.98ALDD179 pKa = 3.9EE180 pKa = 5.04AGLEE184 pKa = 4.43VPEE187 pKa = 4.01EE188 pKa = 4.38WTWDD192 pKa = 3.22EE193 pKa = 4.09YY194 pKa = 11.01MDD196 pKa = 3.9YY197 pKa = 11.31AEE199 pKa = 4.55QLSEE203 pKa = 4.69GEE205 pKa = 4.37GADD208 pKa = 3.28RR209 pKa = 11.84RR210 pKa = 11.84YY211 pKa = 9.7GTFFHH216 pKa = 6.47TWLDD220 pKa = 3.71YY221 pKa = 10.77MKK223 pKa = 10.56LAVFNQPEE231 pKa = 4.17DD232 pKa = 3.89SNIIKK237 pKa = 10.44DD238 pKa = 4.16DD239 pKa = 3.84GVTSNIDD246 pKa = 3.25SEE248 pKa = 4.86HH249 pKa = 5.78IRR251 pKa = 11.84KK252 pKa = 9.51SLEE255 pKa = 3.22IRR257 pKa = 11.84EE258 pKa = 4.46RR259 pKa = 11.84GHH261 pKa = 6.8EE262 pKa = 4.38DD263 pKa = 3.3NSATPYY269 pKa = 11.22ADD271 pKa = 4.14TISLDD276 pKa = 3.51LNYY279 pKa = 9.93RR280 pKa = 11.84DD281 pKa = 3.59QFFGEE286 pKa = 4.32SAAMIMTGTWMINDD300 pKa = 3.56TSGTDD305 pKa = 3.33EE306 pKa = 4.27YY307 pKa = 10.85PPFNLAYY314 pKa = 10.23APYY317 pKa = 8.93PKK319 pKa = 10.19AAEE322 pKa = 4.73GDD324 pKa = 4.08DD325 pKa = 3.78STSPSGADD333 pKa = 3.21FLAIYY338 pKa = 10.15SGSDD342 pKa = 3.45YY343 pKa = 11.14KK344 pKa = 11.25EE345 pKa = 3.52EE346 pKa = 4.47AYY348 pKa = 10.25EE349 pKa = 4.18FIRR352 pKa = 11.84WYY354 pKa = 6.54TTEE357 pKa = 4.82GITMQGKK364 pKa = 9.03YY365 pKa = 10.5LPDD368 pKa = 3.88PQAVDD373 pKa = 2.92IEE375 pKa = 4.47EE376 pKa = 4.33VVNNLLEE383 pKa = 4.34EE384 pKa = 5.03ADD386 pKa = 5.16DD387 pKa = 4.55LDD389 pKa = 4.28QEE391 pKa = 4.67GVNVDD396 pKa = 3.87SLIHH400 pKa = 5.23TLEE403 pKa = 4.18TQEE406 pKa = 4.16PAALNIPPAYY416 pKa = 9.43IGEE419 pKa = 4.3AEE421 pKa = 4.02SAYY424 pKa = 9.79EE425 pKa = 3.93NEE427 pKa = 4.38VEE429 pKa = 5.06SFLLGDD435 pKa = 3.66QDD437 pKa = 5.58LDD439 pKa = 3.86TTIEE443 pKa = 4.07NAHH446 pKa = 6.3EE447 pKa = 4.07AVQNVIDD454 pKa = 4.06QNAEE458 pKa = 3.59

Molecular weight: 51.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H0FN76|A0A1H0FN76_9BACI DNA replication and repair protein RecF OS=Bacillus daliensis OX=745820 GN=recF PE=3 SV=1
MM1 pKa = 7.69GKK3 pKa = 7.98PTFNPNNRR11 pKa = 11.84KK12 pKa = 9.34RR13 pKa = 11.84KK14 pKa = 8.34KK15 pKa = 9.1NHH17 pKa = 4.73GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.37NGRR29 pKa = 11.84KK30 pKa = 9.17VLANRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 8.69GRR40 pKa = 11.84KK41 pKa = 8.69VLSAA45 pKa = 4.05

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3459

0

3459

1040143

25

1926

300.7

33.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.932 ± 0.037

0.603 ± 0.011

5.137 ± 0.036

8.805 ± 0.065

4.432 ± 0.035

6.955 ± 0.042

2.161 ± 0.021

7.084 ± 0.04

6.067 ± 0.047

9.542 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.899 ± 0.018

4.083 ± 0.037

3.701 ± 0.023

3.796 ± 0.027

4.195 ± 0.026

5.945 ± 0.034

5.38 ± 0.026

6.851 ± 0.037

1.067 ± 0.015

3.365 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski