Paraburkholderia dinghuensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Paraburkholderia

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5170 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N6MN98|A0A3N6MN98_9BURK DUF1329 domain-containing protein OS=Paraburkholderia dinghuensis OX=2305225 GN=D1Y85_16685 PE=4 SV=1
LL1 pKa = 7.63DD2 pKa = 3.71NSAGGTLQTSSTDD15 pKa = 3.37LALASATIDD24 pKa = 3.47NDD26 pKa = 3.45GGTITHH32 pKa = 7.49AGTGTLTLVAGSGTGTLSNAGGTIASNGQVAAQAGAFDD70 pKa = 3.99NAAGAVIGQTGLTVTTRR87 pKa = 11.84NTLDD91 pKa = 3.34NTNGRR96 pKa = 11.84LLSNTDD102 pKa = 3.3LSLASSTLNNDD113 pKa = 3.37GGQIGAGANGAIQSGSLTNGGGSITASSLAVTTSGKK149 pKa = 10.22LDD151 pKa = 3.77NGSGDD156 pKa = 3.21IEE158 pKa = 4.42ANQLALNATDD168 pKa = 3.83LVNRR172 pKa = 11.84AGTITQYY179 pKa = 11.0GASPMSVNASGTFDD193 pKa = 3.41NSNGGTLQTNSTDD206 pKa = 3.1VTLAAGTIDD215 pKa = 3.84NDD217 pKa = 3.8GGTITHH223 pKa = 7.33AGAGTLTLDD232 pKa = 4.02ADD234 pKa = 4.06SGAGSLSNAGGTIASNGQVVARR256 pKa = 11.84SGAFGNEE263 pKa = 3.61AGTVIGQTGLSATVGNTLDD282 pKa = 3.66NTNGRR287 pKa = 11.84LLSNADD293 pKa = 3.47LSLASGTLDD302 pKa = 3.67NNGGQIGASANGTVQSGSLANSSGSIAASNLSVTAGTMLDD342 pKa = 3.46NSGGDD347 pKa = 3.17IEE349 pKa = 5.38ANQLALVATNLLNHH363 pKa = 7.07GGAITQYY370 pKa = 8.86GTSPMGFTVSGTFDD384 pKa = 3.27NSNGGTLQANSTDD397 pKa = 3.49LTLAAGTIDD406 pKa = 3.84NDD408 pKa = 3.8GGTITHH414 pKa = 7.49AGTGTLTLDD423 pKa = 3.7AGSGAGSLSNAGGTIASNGQAEE445 pKa = 4.33AQAGAFDD452 pKa = 4.03NAAGTVIGQTGLTATIGNTLDD473 pKa = 3.51NTNGRR478 pKa = 11.84LLSNADD484 pKa = 3.43LSLATGTLTNNGGQIGAAANGTIHH508 pKa = 6.81SGSLTNSGGSMVASNLSVTTATTLDD533 pKa = 3.58NSVGDD538 pKa = 3.72IEE540 pKa = 5.23ANQLALGATDD550 pKa = 4.77LLNHH554 pKa = 7.17GGTITQYY561 pKa = 10.57GASSMGFAVSGTFDD575 pKa = 3.34NSNGGMLQTNSTNLTLAPARR595 pKa = 11.84LDD597 pKa = 3.53NDD599 pKa = 3.43GGAILDD605 pKa = 4.4AGTGTLTIAPGNGAGSISNAGGQIITAGQIAAQAGSLDD643 pKa = 3.65NAGGVLAAQRR653 pKa = 11.84NIAANIAGNVNNTQGAIRR671 pKa = 11.84ALEE674 pKa = 4.11SLSLASGGALTNTNGQIQSGTGAGVGVSDD703 pKa = 5.17ASTLAIQAASIDD715 pKa = 3.86NTGGLVSNLGTGDD728 pKa = 3.36EE729 pKa = 4.53TVQGGSQTVNSGGVITGNGNVAVSTSSLSNTQGGQVSGANVAIQAGTVDD778 pKa = 3.62NSGGAIGTFAGPGGDD793 pKa = 3.43VGITASGTVTNTGGQIGATHH813 pKa = 7.1DD814 pKa = 3.92LSVNAAALTGGGTYY828 pKa = 10.99GAMNDD833 pKa = 3.45VAVTVQGNFTPTPDD847 pKa = 3.47LQFSAGHH854 pKa = 6.75DD855 pKa = 3.58LAFTLPGAFTNSATLDD871 pKa = 3.4AANNLDD877 pKa = 3.64VNANDD882 pKa = 4.23IEE884 pKa = 4.42NSGAMMAGGTLTTHH898 pKa = 6.94SATLEE903 pKa = 4.1NTGVMVGGSVSLNATQTLSNVGPTALIGATDD934 pKa = 3.78SNGLLEE940 pKa = 5.42LLAPDD945 pKa = 4.14IEE947 pKa = 4.48NRR949 pKa = 11.84DD950 pKa = 4.0DD951 pKa = 3.5TTMTDD956 pKa = 3.4TQATTAIYY964 pKa = 10.49GLGAMVLAGGKK975 pKa = 9.12DD976 pKa = 3.32ANGNYY981 pKa = 9.24TNANLIRR988 pKa = 11.84NQSGLIQSAGDD999 pKa = 3.32MTLAANQVTNTRR1011 pKa = 11.84TTMTTTGLNQPVDD1024 pKa = 4.13PDD1026 pKa = 4.25LLTQLGISLSGCTAVQVSDD1045 pKa = 4.7CDD1047 pKa = 3.64PGHH1050 pKa = 7.3PYY1052 pKa = 8.42VQRR1055 pKa = 11.84YY1056 pKa = 7.88QPDD1059 pKa = 3.46NPVFLTMIGGAFTMPPRR1076 pKa = 11.84SGQWNSNYY1084 pKa = 10.11QNTTYY1089 pKa = 10.54TGVALANLIASISPQAQIIAGGNLDD1114 pKa = 3.49ASKK1117 pKa = 11.16VGLFQNYY1124 pKa = 5.82WSAVAAVGNIALPVTLDD1141 pKa = 3.17QDD1143 pKa = 3.32SWQGQTAPGVQVTYY1157 pKa = 10.49SGYY1160 pKa = 9.4YY1161 pKa = 9.75HH1162 pKa = 6.6YY1163 pKa = 11.55ANYY1166 pKa = 9.87DD1167 pKa = 3.07QSIRR1171 pKa = 11.84DD1172 pKa = 3.39WTLPFGDD1179 pKa = 4.25APFVGSNPGGYY1190 pKa = 7.19TQAAPADD1197 pKa = 3.77VRR1199 pKa = 11.84TYY1201 pKa = 11.33ALPSYY1206 pKa = 10.18EE1207 pKa = 4.22STFVAGGTISGTGVSINNTAGNAGVPSLGLVPGQSVGGVNAGGVSGSADD1256 pKa = 2.95GSAAGGTSAIQGSAGGSNTGATSVVQGSAGSPDD1289 pKa = 3.17SAAASVSGSTGLTNPVIASATAVNVLSNLTIPQGGLFRR1327 pKa = 11.84PATAPNAQYY1336 pKa = 10.93LIEE1339 pKa = 4.42TNPAFTSQKK1348 pKa = 10.72SFISSDD1354 pKa = 3.75YY1355 pKa = 10.82YY1356 pKa = 11.06LQQLGLNPQTTEE1368 pKa = 3.48KK1369 pKa = 10.88RR1370 pKa = 11.84LGDD1373 pKa = 3.66GLYY1376 pKa = 9.89EE1377 pKa = 4.06QQLVV1381 pKa = 3.4

Molecular weight: 134.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N6N6N1|A0A3N6N6N1_9BURK Rod shape-determining protein MreD OS=Paraburkholderia dinghuensis OX=2305225 GN=mreD PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVTRR11 pKa = 11.84RR12 pKa = 11.84KK13 pKa = 8.0RR14 pKa = 11.84THH16 pKa = 5.76GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 8.74TAGGRR28 pKa = 11.84KK29 pKa = 9.04VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.58GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5170

0

5170

1685335

26

3298

326.0

35.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.585 ± 0.048

0.957 ± 0.01

5.457 ± 0.027

5.171 ± 0.032

3.682 ± 0.021

8.205 ± 0.035

2.329 ± 0.015

4.711 ± 0.021

3.057 ± 0.032

10.033 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.356 ± 0.016

2.901 ± 0.024

5.09 ± 0.029

3.638 ± 0.024

6.9 ± 0.04

5.71 ± 0.051

5.55 ± 0.033

7.833 ± 0.027

1.394 ± 0.016

2.441 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski