Nocardiopsis alba (strain ATCC BAA-2165 / BE74)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Streptosporangiales; Nocardiopsaceae; Nocardiopsis; Nocardiopsis alba

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5500 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|J7LIH7|J7LIH7_NOCAA Branched-chain amino acid transport system / permease component family protein OS=Nocardiopsis alba (strain ATCC BAA-2165 / BE74) OX=1205910 GN=B005_3878 PE=4 SV=1
MM1 pKa = 7.49IKK3 pKa = 10.35GVTMFEE9 pKa = 3.98RR10 pKa = 11.84RR11 pKa = 11.84KK12 pKa = 8.19TLALAAAATSTVLVVGGCGSGDD34 pKa = 3.33GGGGDD39 pKa = 3.86GGPKK43 pKa = 9.48EE44 pKa = 4.37VTWVINSLPGAWQATSLAGGSVYY67 pKa = 10.15VVQMLSGVLPYY78 pKa = 9.97TGQWQPDD85 pKa = 3.36GTYY88 pKa = 9.86EE89 pKa = 4.23YY90 pKa = 11.25DD91 pKa = 3.48MNVLAEE97 pKa = 4.25EE98 pKa = 4.78PEE100 pKa = 5.01LINDD104 pKa = 4.82DD105 pKa = 3.88PDD107 pKa = 3.56EE108 pKa = 5.39GPFEE112 pKa = 4.09WSITLAEE119 pKa = 4.28DD120 pKa = 4.06AVWSDD125 pKa = 3.73GEE127 pKa = 4.3PMTGEE132 pKa = 4.31DD133 pKa = 5.37LRR135 pKa = 11.84VSWMLGTSPDD145 pKa = 3.11EE146 pKa = 4.98GYY148 pKa = 10.91CGGCDD153 pKa = 3.3PRR155 pKa = 11.84AAASFDD161 pKa = 3.37KK162 pKa = 11.23VEE164 pKa = 4.25SVEE167 pKa = 4.54ADD169 pKa = 2.92GKK171 pKa = 8.97TATFQLKK178 pKa = 10.2EE179 pKa = 4.0GLADD183 pKa = 4.08PEE185 pKa = 4.31WMSLFDD191 pKa = 3.83AHH193 pKa = 6.22SQSGGFLPAHH203 pKa = 6.49LAEE206 pKa = 4.4EE207 pKa = 4.52NGWDD211 pKa = 4.83VDD213 pKa = 4.61DD214 pKa = 5.61ADD216 pKa = 4.86QLGEE220 pKa = 4.18YY221 pKa = 10.01YY222 pKa = 10.64DD223 pKa = 3.57WLHH226 pKa = 6.76AEE228 pKa = 4.08RR229 pKa = 11.84PEE231 pKa = 4.25WSGGPYY237 pKa = 9.74MITAGDD243 pKa = 3.95LEE245 pKa = 4.58NEE247 pKa = 4.61VVKK250 pKa = 10.64EE251 pKa = 3.86PNPNYY256 pKa = 10.33FGEE259 pKa = 4.45EE260 pKa = 3.88PLLDD264 pKa = 5.26KK265 pKa = 10.39ITMPYY270 pKa = 8.42NTDD273 pKa = 2.65EE274 pKa = 4.34GTFVNAFINGEE285 pKa = 3.85IDD287 pKa = 3.17GGNPADD293 pKa = 3.87YY294 pKa = 10.93SEE296 pKa = 5.46DD297 pKa = 3.94VILQLQDD304 pKa = 3.13VANAEE309 pKa = 4.06VTIAEE314 pKa = 4.49GATWEE319 pKa = 4.59HH320 pKa = 6.46VDD322 pKa = 5.03LNLKK326 pKa = 10.38NEE328 pKa = 4.16TLQDD332 pKa = 3.44VEE334 pKa = 4.07LRR336 pKa = 11.84RR337 pKa = 11.84AIFTAIDD344 pKa = 3.45RR345 pKa = 11.84DD346 pKa = 4.37DD347 pKa = 3.41IANRR351 pKa = 11.84NFGAGYY357 pKa = 9.49PDD359 pKa = 4.23YY360 pKa = 10.47EE361 pKa = 4.63LKK363 pKa = 10.82NNHH366 pKa = 5.4VFGSDD371 pKa = 2.76SPYY374 pKa = 10.92YY375 pKa = 9.3VDD377 pKa = 3.96HH378 pKa = 7.46FEE380 pKa = 5.86GEE382 pKa = 4.42TQGTGDD388 pKa = 3.32IDD390 pKa = 3.67EE391 pKa = 4.99AKK393 pKa = 10.66SILEE397 pKa = 3.91EE398 pKa = 4.6AGYY401 pKa = 10.42EE402 pKa = 3.97LDD404 pKa = 5.27GGTLTLDD411 pKa = 3.78GEE413 pKa = 4.81EE414 pKa = 4.03IGPFRR419 pKa = 11.84LRR421 pKa = 11.84STDD424 pKa = 2.99STVRR428 pKa = 11.84NTSVQLIQAQLSEE441 pKa = 4.28IGITTNIEE449 pKa = 3.83MTDD452 pKa = 3.57DD453 pKa = 3.87LGGMLAAAEE462 pKa = 4.09YY463 pKa = 10.74DD464 pKa = 3.09IVQYY468 pKa = 10.53GWSGSPYY475 pKa = 8.93FTGNPDD481 pKa = 3.61QFWHH485 pKa = 6.38SEE487 pKa = 3.93SGSNFGGYY495 pKa = 10.48SNDD498 pKa = 3.74EE499 pKa = 4.05VDD501 pKa = 5.55DD502 pKa = 4.97LADD505 pKa = 3.53QVANAPDD512 pKa = 4.51LDD514 pKa = 4.17TAAEE518 pKa = 4.07HH519 pKa = 6.81ANAAMEE525 pKa = 4.09ILVPEE530 pKa = 4.94AYY532 pKa = 9.98VLPIMAEE539 pKa = 3.82PNYY542 pKa = 10.2FFANSDD548 pKa = 3.55RR549 pKa = 11.84LVNVHH554 pKa = 7.4DD555 pKa = 4.7NLQSSYY561 pKa = 10.71RR562 pKa = 11.84ATYY565 pKa = 10.4NIGEE569 pKa = 4.22WDD571 pKa = 3.58VAGG574 pKa = 4.12

Molecular weight: 62.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|J7L8U2|J7L8U2_NOCAA YcfA-like family protein OS=Nocardiopsis alba (strain ATCC BAA-2165 / BE74) OX=1205910 GN=B005_2467 PE=3 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.45VHH17 pKa = 5.31GFRR20 pKa = 11.84LRR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIIASRR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 9.5GRR40 pKa = 11.84AALTVSHH47 pKa = 7.07

Molecular weight: 5.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5500

0

5500

1675371

37

3832

304.6

32.89

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.691 ± 0.042

0.707 ± 0.009

6.261 ± 0.034

7.046 ± 0.037

2.834 ± 0.02

9.527 ± 0.037

2.336 ± 0.017

3.485 ± 0.026

1.617 ± 0.029

10.383 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.971 ± 0.014

1.744 ± 0.019

6.12 ± 0.035

2.149 ± 0.018

8.587 ± 0.038

5.521 ± 0.023

5.915 ± 0.027

8.679 ± 0.034

1.472 ± 0.015

1.956 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski